Electroacupuncture at Dazhui(GV14) and Mingmen(GV4) protects against spinal cord injury:the role of the Wnt/β-catenin signaling pathway

Electroacupuncture at Dazhui（GV14） and Mingmen（GV4） on the Governor Vessel has been shown to exhibit curative effects on spinal cord injury; however, the underlying mechanism remains poorly understood. In this study, we established rat models of spinal cord injury using a modified Allen＇s weight-drop method. Ninety-nine male Sprague-Dawley rats were randomly divided into three equal groups： sham（only laminectomy）, SCI（induction of spinal cord injury at T10）, and EA（induction of spinal cord injury at T10 and electroacupuncture intervention at GV14 and GV4 for 20 minutes once a day）. Rats in the SCI and EA groups were further randomly divided into the following subgroups： 1-day（n = 11）, 7-day（n = 11）, and 14-day（n = 11）. At 1, 7, and 14 days after electroacupuncture treatment, the Basso, Beattie and Bresnahan locomotor rating scale showed obvious improvement in rat hind limb locomotor function, hematoxylin-eosin staining showed that the histological change of injured spinal cord tissue was obviously alleviated, and immunohistochemistry and western blot analysis showed that Wnt1, Wnt3 a, β-catenin immunoreactivity and protein expression in the injured spinal cord tissue were greatly increased compared with the sham and SCI groups. These findings suggest that electroacupuncture at GV14 and GV4 upregulates Wnt1, Wnt3 a, and β-catenin expression in the Wnt/β-catenin signaling pathway, exhibiting neuroprotective effects against spinal cord injury.

Catgut implantation at acupoints increases the expression of glutamate aspartate transporter and glial glutamate transporter-1 in the brain of rats with spasticity after stroke

Catgut implantation at acupoints has been shown to alleviate spasticity after stroke in rats.However,the underlying mechanisms are poorly understood.In this study,we used the rat middle cerebral artery occlusion model of stroke.Three days after surgery,absorbable surgical catgut sutures were implanted at Dazhui（GV14）,Jizhong（GV6）,Houhui,Guanyuan（CV4）and Zhongwan（CV12）.The Zea Longa score was used to assess neurological function.The Modified Ashworth Scale was used to evaluate muscle tension.The 2,3,5-triphenyl-tetrazolium chloride assay was used to measure infarct volume.Immunohistochemical staining was performed for glutamate aspartate transporter（GLAST）and glial glutamate transporter-1（GLT-1）expression.Western blot assay was used to analyze the expression of GLAST and GLT-1.Reverse transcription and polymerase chain reaction were carried out to assess the expression of GLAST and GLT-1m RNAs.After catgut implantation at the acupoints,neurological function was substantially improved,muscle tension was decreased,and infarct volume was reduced in rats with spasticity after stroke.Furthermore,the expression of GLAST and GLT-1 m RNAs was increased on the injured（left）side.Our findings demonstrate that catgut implantation at acupoints alleviates spasticity after stroke,likely by increasing the expression of GLAST and GLT-1.

Effect of stimulating the acupoints Feishu (BL 13) and Dazhui (GV14) on transdermal uptake of sinapine thiocyanate in asthma gel

OBJECTIVE: To investigate the effect of stimulating the acupoints Feishu(BL 13) and Dazhui(GV 14) on the transdermal uptake of sinapine thiocyanate contained in a gel used for the management of asthma.METHODS: Thirty Sprague-Dawley rats were randomly divided into three equal groups using a random number table: the Feishu(BL 13) acupoint group, the Dazhui(GV 14) acupoint group, and the nonacupoint group or control group. Using micro-dialysis technology, preprocessed skin probes were implanted into the rats at Feishu(BL 13), Dazhui(GV 14), and a nonacupoint site. Asthma gel was then placed on the skin at Feishu(BL 13), Dazhui(GV14) acupoints, and the nonacupoint for all groups.Dialysate was collected every 30 min for 12 h. The normalized concentration of sinapine thiocyanate in the skin was determined by high-performance liquid chromatography.RESULTS: The rat in vivo transdermal experiment demonstrated that the quantity-time equation showed a good linear correlation with zero-order kinetics(r > 0.99). The transdermal behavior was in accordance with the first-order rate open model in which the transdermal penetration rates and the accumulative amounts of sinapine thiocyanate in the skin at the acupoint sites were greater than those through the skin of the nonacupoint site. The systemic maximum concentration and the area under the curve of sinapine thiocyanate in the acupoint groups were significantly greater than in the nonacupoint group. A lag time was observed in both acupoint groups, but not in the nonacupoint group.CONCLUSION: Stimulating the acupoints promotes the percutaneous absorption of sinapine thiocyanate and also controls its release, reducing concentration fluctuations in the blood.

针挑治疗颈椎病的随机对照研究

目的:运用随机对照临床研究方法,对针挑治疗颈椎病进行疗效评价。方法:将158例患者随机分为针挑治疗组(针挑组)56例、常规针刺对照组(针刺组)55例与局麻对照组(局麻组)47例。取穴均为颈百劳、大椎、肩井等,分别采用针挑法、常规针刺法及局部麻醉法治疗。采用简化McGill疼痛询问量表等量化计分,结合临床症状、体征进行疗效分析。结果:针挑组痊愈率为57.1%,优于针刺组的23.6%与局麻组的14.9%(均P<0.01),针挑治疗基本无不良反应。结论:针挑疗法对于颈椎病是一种高效、安全的疗法。

大椎、关元对腰椎间盘突出症临床疗效的影响

目的:探讨大椎、关元对腰椎间盘突出症临床疗效的影响,以及任督脉在腰椎间盘突出症临床治疗上的指导意义。方法:将427例腰椎间盘突出症患者随机单盲分为常规(循经取穴)组、常规加大椎组和常规加关元组进行治疗观察,采用u检验评定比较各组间疗效。结果:加大椎组和加关元组的治愈率与常规组比较,差异有显著或非常显著性意义(P<0.05,P<0.01),而有效率组间比较差异无显著性意义(P>0.05)。结论:大椎、关元可以提高腰椎间盘突出症的治愈率,调节任督、温补阳气可能是治疗腰椎间盘突出症的重要手段和方法之一。

针刺大椎治疗感冒高热临床观察

目的:观察针刺退热的临床疗效。方法:将86例高热患者随机分为两组,针刺组45例,针刺大椎;药物组41例,肌肉注射安痛定注射液。观察治疗前后24小时内患者的相关症状的改善和体温变化,采用单盲、前后对照的方法。结果:针刺组痊愈9例,药物组痊愈2例。针刺组退热效果明显优于药物组。结论:针刺退热疗效可靠,值得临床推广应用。

青鹏膏剂穴位贴敷对肥大细胞数量及脱颗粒的影响

目的:观察体表穴区贴敷青鹏膏剂后,大鼠穴区内肥大细胞(MCs)的分布以及数量和脱颗粒的变化,了解穴位贴敷对皮肤组织的影响,研究其发挥作用的可能性机理。方法:健康Wistar大鼠30只,随机分为空白对照组(A组)、电针组(B组)和青鹏膏剂贴敷组(C组),每组10只。干预组动物给予青鹏膏剂贴敷或电针刺激7 d后,分别取3组动物的"大椎"穴区皮肤组织块做冰冻切片,经亚铁氯化铜法示乙酰胆碱酯酶并甲苯胺兰复染,比较3组"大椎"穴区组织MCs分布、数量及脱颗粒的差异,研究穴区内MCs的形态学改变。结果:各组"大椎"穴区真皮、皮下组织和肌肉组织中都分布着大小、数量不等的MCs,以皮下组织层分布最为广泛,多沿神经或血管分布。与A组比较,青鹏膏剂贴敷组穴区组织局部MCs数量及脱颗粒率显著升高,差异有显著性(P<0.05)。结论:青鹏膏剂穴位贴敷能够明显引起皮肤组织内MCs数量增多及脱颗粒,有促聚集作用,是穴位贴敷与经络、药物协同增效的可能性因素之一。

艾灸大椎、阳陵泉速降血沉211例临床观察

艾灸大椎、阳陵泉速降血沉２１１例临床观察吴炳煌，林燕（福建中医学院针推系，福州３５０００３；福建省人民医院）主题词血沉／针灸效应，穴．大椎，穴．阳陵泉，艾条灸，风湿病／针灸疗法ＣｌｉｎｉｃａｌＯｂｓｅｒｖａｔｉｏｎｏｎ２１１ＣａｓｅｓＴｒｅａｔｅｄｂ...

针刺大椎对感冒高热退热效果的临床观察

目的:探讨大椎穴对感冒高热的治疗作用。方法:将261例患者随机分为治疗组(133例)和对照组(128例)。治疗组采用电针大椎,对照组采用肌肉注射安痛定注射液,观察24小时的即时退热效果。结果:治疗组治疗后各时点体温均低于对照组(P<0.01)。治疗组起效时间为(1.42±1.79)小时,短于对照组的(3.44±5.10)小时(P<0.01)。治疗组痊愈率为27.8%,解热率为75.9%;对照组痊愈率为10.9%,解热率为55.5%,两组比较差异均有非常显著性意义(P<0.01)。风热证感冒治疗组的解热率为75.3%,对照组为50.0%,两组比较差异有非常显著性意义(P<0.01)。结论:大椎穴退热疗效确切,风热感冒所致高热可首选大椎,治疗应越早越好。

大椎穴点刺加拔罐治疗颅内高压综合征40例

目的:观察大椎穴点刺加拔罐对静脉点滴甘露醇患者脑脊液压力的影响。方法:80例患者随机分为治疗组和对照组。治疗组用静脉点滴甘露醇加大椎穴点刺拔罐治疗,对照组静脉点滴甘露醇治疗。结果:治疗组显效率(45.0％)明显高于对照组(17.5％),总有效率治疗组(82.8％)也明显高于对照组(60.0％),两组之间比较差异有非常显著性意义(P<0.01)。结论:大椎穴点刺加拔罐配合治疗对颅内高压综合征有良好的效果。

穴位敷贴治疗梅核气(肝郁气滞型)30例

目的观察中药穴位敷贴治疗梅核气(肝郁气滞型)的临床疗效。方法对30例梅核气(肝郁气滞型)患者,选取疏肝理气、散结解郁的中药按比例研末,并以适量蜂蜜或醋调匀,制成直径约2 cm,厚度约0.3cm的药膏,用胶布将块状药膏贴于大椎、天突、膻中穴位上进行治疗。结果治愈17例,有效9例,无效4例,总有效率86.67%。结论中药穴位敷贴治疗梅核气(肝郁气滞型),可疏通经络,调节人体内分泌功能,疏肝理气、散结解郁,调节全身气血阴阳,明显改善临床症状。

大椎穴针刺安全深度的临床研究

目的 :探讨大椎穴针刺安全深度。方法 :将 4 0例颈椎间盘突出症患者 ,采用大椎穴处硬脊膜外穿刺 ,测量硬膜外深度 ,即大椎穴安全深度。结果 :穿刺针依次经过皮肤、筋膜、棘上韧带、棘间韧带、黄韧带至硬膜外腔 ,深度约 36～ 75mm ,平均深度为 5 4 6mm。结论 :建议大椎穴针刺深度应控制在 36mm以内。

扬刺大椎穴为主治疗颈椎病疗效观察

目的 :探寻治疗颈椎病的最佳治疗方法。方法 :将 780例颈椎病患者随机分为扬刺大椎穴为主治疗组、针刺颈夹脊穴为主对照 1组、牵引按摩对照 2组各 2 6 0例 ,观察治疗 2个疗程的疗效及停止治疗 1年后的远期疗效。结果 :治疗组疗效稳定 ,明显优于对照组 (P <0 0 0 5 )。结论 :扬刺大椎穴为主治疗颈椎病疗效最佳 。

Effect of catgut implantation at acupoints on GABA_B and mGluR1 expressions in brain stem of rats with spasticity after stroke

OBJECTIVE: To investigate the effect of catgut implantation at acupoints on the expressions of γ-amino butyric acid B receptor(GABAB ) and metabotropic glutamate receptor 1(mGluR1) in the brain stem of rats with spasticity after stroke.METHODS: In total, 60 male Sprague-Dawley rats were randomly divided into three groups: a sham group(n=10), a model group(n=25) and a treatment group(n=25). The rats in both the model group and the treatment group were subjected to middle cerebral artery occlusion to establish a model of focal cerebral ischemia. Rats with limb-spasm met the inclusion criteria. Only the left carotid artery was isolated in sham group rats. Three days after modeling, the treatment group was subjected to catgut implantation at Dazhui(GV 14), Guanyuan(CV 4), and Zhongwan(CV 12). Neurological deficit symptoms were assessed with the Zea-Longa neurological deficit score. The Modified Ashworth Scale(MAS), and isolated muscle tone were used to evaluate spasticity before and after treatment. Immunohistochemistry was applied to determine the expression of GABAB and mGluR1 in the rat brain stem after treatment.RESULTS: After treatment, neural impairment symptoms had significantly improved in the treatment group when compared to the model group(P<0.05). Both MAS and isolated muscle tone in the treatment group were significantly decreased when compared with the model group(P<0.05),and were also lower than before treatment. GABAB expression was significantly higher and mGluR1 was lower in the treatment group when compared with the model group(P<0.01 and P<0.05, respectively).CONCLUSION: Catgut implantation at Dazhui(GV 14), Guanyuan(CV 4), and Zhongwan(CV 12), can relieve limb spasticity by increasing the expression of GABAB and reducing the expression of mGluR1 in the brain stem of rats after stroke.

Functional Feature of“Five Yang Acupoints”in Governor Vessel

Governor Vessel is one of eight extraordinary Meridians, and been also called ＂the sea of Yang Meridians＂. It masters the Yin and Yang of the body cooperating with Conception Vessel. Acupoints on Governor Vessel have been used widely in clinic. The author expounded the functional feature of Yaoyangguan （GV 3）, Mingmen （GV 4）, Zhiyang （GV 9）, Dazhui （GV 14） and Baihui （GV 20）, which can show the characteristic of Governor Vessel and provide reference for clinical application.

针刺大椎穴对椎动脉型颈椎病患者脑干听觉诱发电位的影响

目的:研究大椎穴治疗椎动脉型颈椎病的作用机制,并探索以脑干听觉诱发电位(BAEP)作为椎动脉型颈椎病的疗效评定客观指标的可行性。方法:对52例椎动脉型颈椎病患者采取针刺大椎治疗,观察其临床疗效以及治疗前后BAEP变化情况。结果:治愈17例,好转26例,未愈9例,有效率达82.7%。BAEP中ⅤPL、Ⅰ-ⅢIPL、Ⅲ-ⅤIPL在治疗前后差异有显著性意义(P<0.05),临床有效组(治愈和好转)与无效组(未愈)对比差异有显著性或非常显著性意义(P<0.05,P<0.01)。结论:针刺大椎穴可改善椎-基底动脉的供血情况,使椎动脉型颈椎病的临床症状消失或减轻。BAEP可作为椎动脉型颈椎病的疗效评定客观指标。

Treatment and Prevention of 100 Cases with Allergic Rhinitis by Acupoint Sticking Therapy

Objective: To observe the clinical effects of acupoint sticking therapy in the three ten-day periods in summer and three nine-day periods in winter. Methods: Herbal medicines were prepared into crude herbal powder and made into paste with ginger juice, and were respectively applied to the corresponding acupoints in the three ten-day periods in summer and three nine-day periods in winter. Results: The results showed 45 cases were cured, 50 cases were improved, and 5 cases failed out of 100 cases, with the total effective rate of 95%. Conclusion: The acupoint sticking therapy for the treatment and prevention of allergic rhinitis is simple in method, remarkable in effects, acceptable to the patients, and is worthy of clinical popularization.

关于督脉大椎和腰阳关腧穴定位的探讨

自1915年汉译日本针灸医籍《最新实习西法针灸》和1931年《高等针灸学讲义》中对腧穴解剖位置的描述开始,近1个世纪以来,大椎位于第7颈椎棘突下、腰阳关位于第4腰椎棘突下很少有人质疑。笔者查阅古代文献,结合现代解剖学知识,并通过对针灸明堂图和针灸铜人这一核心论据进行求证,认为大椎的定位为第1胸椎棘突下、腰阳关位于第5腰椎棘突下,由此,督脉大椎到腰阳关、足太阳膀胱经第一侧线大杼到白环俞和第二侧线附分到秩边的所有穴位的定位均应下移1个椎体。

耳熏灸联合热敏灸装置的研制与特点

设计一款耳熏灸联合热敏灸的艾灸装置,可实现利用艾条燃烧时产生的艾烟对患者外耳道进行艾烟熏灸治疗,同时利用艾条燃烧时产生的热量对大椎穴进行热敏灸治疗。本装置由燃烧室、导烟管、艾烟处理室、动力模块以及连接件5个部分组成,可以解决传统艾烟熏灸和热敏灸过程中治疗体验欠佳、温控差、易烫伤以及人工依赖性高的问题,能提高熏灸和热敏灸治疗的安全性、便捷性以及医务人员的工作效率。

艾灸对偏头痛大鼠血清β-EP、SP含量及脑干IL-1β、COX-2蛋白表达的影响

目的:观察不同时间艾灸“百会”“大椎”对偏头痛大鼠血清β-内啡肽(β-EP)、P物质(SP)含量及脑干白介素-1β(IL-1β)、环氧合酶2(COX-2)蛋白表达的影响,探讨艾灸防治偏头痛的作用及机制。方法:将40只雄性SD大鼠随机分为空白组、模型组、预防+治疗组和治疗组,每组10只。除空白组外,其他各组大鼠采用硝酸甘油皮下注射制备偏头痛模型。预防+治疗组于造模前7 d(每日1次)及造模后30 min予艾灸干预,治疗组于造模后30 min予艾灸干预,均穴取“百会”“大椎”,每次30 min。观察各组大鼠造模前后行为学评分。干预结束后,采用ELISA法检测各组大鼠血清β-EP、SP含量,免疫组化法检测各组大鼠脑干IL-1β阳性细胞数,Western blot法检测各组大鼠脑干COX-2蛋白表达。结果:与空白组比较,模型组大鼠造模后0~30 min、60~90 min、90~120 min行为学评分升高(P<0.01);与模型组比较,治疗组、预防+治疗组大鼠造模后60~90 min、90~120 min行为学评分降低(P<0.01)。与空白组比较,模型组大鼠血清β-EP含量降低(P<0.01),血清SP含量、脑干IL-1β阳性细胞数及COX-2蛋白表达均升高(P<0.01);与模型组比较,预防+治疗组和治疗组大鼠血清β-EP含量升高(P<0.01),血清SP含量、脑干IL-1β阳性细胞数及COX-2蛋白表达均降低(P<0.01,P<0.05);与治疗组比较,预防+治疗组大鼠血清β-EP含量升高、COX-2蛋白表达降低(P<0.05)。结论:艾灸可有效缓解偏头痛。其机制可能为降低大鼠血清SP含量、脑干IL-1β和COX-2蛋白表达,升高血清β-EP含量,预防+治疗干预的效果最佳。