Efficacy of static magnetic field for pain of adjuvant arthritis rats

Rats suffering from adjuvant arthritis (AA) were used to examine the effect of a static magnetic field (SMF) upon pain relief. Rats were divided into SMF- treated AA rats, non-SMF treated AA rats and control rats. Following SMF stimulation, we measured blood flow volume in the paw and then reactive speed response to thermal stimulation. The AA groups exhibited significantly lower blood volume and reactivity to thermal stimulation compared to the control group. Compared to non-SMF, SMF exhibited increased blood flow volume in both the tail and paw, along with an increased reactive speed response to thermal stimulation. Our findings suggest that an improved of blood flow and reactive speed response, induced by SMF, appears to be effective for the relief of pain induced by chronic inflammation.

Observation on the Therapeutical Effect of Adjuvant Arthritis in Rats with Medicinal Indirect Moxibustion

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Effect and mechanism of N-acetylcysteine on adjuvant arthritis rats

Objective:To investigate the anti-inflammatory effects of N-Acetylcysteine(NAC)on adjuvant arthritis(AA)rats and the changes of Toll-like receptor 2(TLR2)-inflammatory factors.Methods:50 SD rats,randomly divided into 10 control groups,40 AA model group,after 10 d,which were divided into model group,NAC groups gave low,medium and high dose NAC(lavage respectively 50,100,200 mg/kg)for 16 d,joint swelling degree and arthritis index evaluation NAC therapeutic effect on AA rats;Immunohistochemical staining and Western blot were used to observe the expression of TLR2 protein in synovial tissue of joints.Serum levels of TNF-α,IL-1βand IL-10 were detected by ELISA.Results:Compared with the control group,the joint swelling and arthritis index in the model group were significant,and the joint swelling and arthritis index in the NAC treatment groups were significantly reduced(P<0.05,P<0.01).The expression of TLR2 protein in the synovial tissue of NAC treatment groups was decreased,inflammatory factors TNF-αand IL-1βwere decreased,and IL-10 was increased(P<0.01).Conclusion:NAC can improve the symptoms of arthritis in AA rats,and its mechanism may be related to inhibition of TLR2 and regulation of downstream inflammatory factor pathways in AA rats.

Protective Effect and Mechanism of n-butanol Extract from Diploclisia glaucescens(B1.)Diels on Rats with Adjuvant Arthritis

[Objectives]To study the protective effect and mechanism of n-butanol extract of Diploclisia glaucescens(B1.)Diels on rats with adjuvant arthritis.[Methods]A rat adjuvant arthritis(AA)model with similarities to a clinical RA(rheumatoid arthritis)patient was used,and the model was made by injection of Complete Freund s adjuvant(CFA).Body mass and joint swelling degree were used as indicators,and the organ index was calculated and the synovial tissue of rats was examined under microscope to evaluate the protective effect of n-butanol extract on arthritis.The effects of n-butanol extract on TNF-α,IL-1βand PGE_(2)contents in rat serum were detected by ELISA kit.[Results]Arthritic rats experienced significant weight loss;the n-butanol extract reduced the joint swelling in rats.It exerted an effect on rat organs and reduced the contents of TNF-α,IL-1βand PGE_(2) in rat serum,and also reduced synovial inflammation in rats.[Conclusions]The n-butanol extract of D.glaucescens can protect rats with adjuvant arthritis by reducing the content of inflammatory factors.

Effect of Tripterygium Vilfordii on Adrenal Cortex in Rat with Adjuvant Arthritis

This paper reports the effects of Tripterygium Wilfordii (TW) on adrenal cortex in rats with adjuvant artbritis. Forty rats were divided into 5 groups. Adjuvant arthritis (AA) models were made with complete freund's adjuvant (CFA) in groups Ⅰ-Ⅳ. Each of which was treated with sodium carboxyl methyl cellulose, TW, prednisone and cyclophosphamide respecrively.The untreated rats allocated to group,V served as normal. controls. The swelling of AA markedly subsided in groups Ⅱ,Ⅲ and Ⅳ as compasred with group I (P<0.01). whereas no significant differences were noted among groups Ⅱ, Ⅲ and Ⅳ(P>0.05).The obviously increased plasma corticosterone levels and decreased adrenal ascorbic acid levels were observed in group Ⅱ,whereas decreased plasma corticosterone levels and inereased adrenal ascorbic acid levels were noted in group Ⅲ.There was a striking comtrast between groups Ⅱand Ⅲ. The morphological changes of adrenal glands under light microseope revealed hypertrophic adrenal cortices in group Ⅱ,and atrophic adrenal cortices in group Ⅲ.The above findings snggest that that the effect promoting production of corticosterods may be one of mechanism by which TW can effectively treating autoimmune diseases.

Anti-inflammatory effects of Solanum procumbens on a low dose complete Freund's adjuvant-induced arthritis rat model

Objective:To investigate the anti-inflammatory and analgesic effects of Solanum procumbens on complete Freund’s adjuvantinduced arthritis rat models.Methods:We isolated and identified five compounds in the ethanolsoluble Solanum procumbens extract(SP)with anti-inflammatory effects,including ursolic acid,β-sitosterol,hexadecanoic acid,cisvaccenic acid,and vanillic acid.Additionally,we investigated the anti-inflammatory effects of SP on rheumatoid arthritis symptoms,including paw volumes,local temperatures,withdrawal latency,and mechanical withdrawal threshold at the hind paw and white blood cell(WBC)number from complete Freund’s adjuvant-induced arthritis rat models.Results:We have successfully established a complete Freund’s adjuvant-induced arthritis rat model at a low dose(1 mg/mL).SP extract significantly reduced paw volumes(P<0.05),prolonged withdrawal latencies(P<0.05),decreased local temperature,and increased the mechanical withdrawal threshold(P<0.05),but only SP extract at the dose of 300 mg/kg significantly decreased WBC numbers.Conclusions:SP extract could be a potential medication candidate with anti-inflammatory effects for arthritis,but it requires further investigation into the mechanism of the SP and its effectiveness on other models as well as clinical trials.

Biophoton Imaging Evaluation of the Process of Rheumatoid Arthritis in Rats

Rheumatoid arthritis (RA) is a common form of chronic inflammatory arthritis, and it mainly causes the destruction of small joints. The development of this disease is a relatively secret and repeated process, and therefore early diagnosis and evaluation of the disease is usually difficult. In this study, an arthritis model was successfully induced by injecting complete Freund’s adjuvant (CFA) into the toes of lower limbs of Wistar rats. Seven days after injection of CFA, obvious redness and swelling appeared at the toe joints of lower limbs accompanied by more sensitivity to thermal stimulation. Using the ultraweak bio-photon imaging system (UBIS) established by us, the toe joint area of the lower limbs of rats was imaged 7 days after injection of CFA. It was found that the volar part of lower limbs of arthritis rats showed significantly higher biophoton emissions compared with the control group. The results of this study may provide a basis for further research and devel-opment of early diagnosis and assessment of lesion progression of rheumatoid arthritis.

Improving Effects of Astragalus Polysaccharides on Cardiac Function via Keap1/Nrf2-ARE Signal Pathway in Adjuvant Arthritis Rats

Objective To observe the effect of Astragalus polysaccharides（APS） on cardiac function and expression of Keap1/Nrf2-ARE signal pathway in adjuvant arthritis（AA） rats. Methods Forty-eight rats were randomly divided into normal control（NC） group, model control（MC） group, leflunomide（LEF） group, and APS group. The AA model rats were induced with Freund＇s complete adjuvant, then administration began from day 19 after modeling for 30 d. The paw swelling and arthritic index（AI）, the cardiac function indexes, the expression of Keap1, maf, Nrf2 m RNAs, and HO-1, r-GCS proteins in cardiac tissues were observed. Oxidation-related substances（SOD, MDA, ROS, and TAC） and cytokines（IL-10 and TNF-ɑ） were also determined. Results Compared with NC group, the paw swelling and AI were increased, and the body weight was decreased in MC group. HR, HI, LVSP, LVEDP, and levels of MDA, TAC, ROS, RNS, and TNF-ɑ in MC group were increased, while dp/dtmax and levels of GSH, TRX, and IL-10 were decreased. Compared with MC group, LEF group showed higher HR and ± dp/dtmax, and lower LVEDP. In APS group, the HR, LVSP, and LVEDP were decreased and ±dp/dtmax was increased. TNF-ɑ was decreased, TAC, ROS, MDA, and IL-10 were increased in both LEF and APS groups. APS and LEF groups also showed less MDA, TAC, ROS, and SOD, and the differences were statistically significant. Spearman correlation analysis showed that the cardiac function parameters were positively correlated with Keap1, anti-oxidant indicators, and anti-inflammation cytokines, and negatively correlated with Nrf2, oxidation indictors, pro-inflammation cytokines, and inflammation indictors.Conclusion APS can adjust the expression of Keap1/Nrf2-ARE signal pathway in AA rats, and can also improve their cardiac function. The mechanism may be involved in increasing myocardial anti-oxidant capacity, reducing oxidative stress, and inhibiting inflammation.

Effects of cryptotanshinone on immune functions in rats with adjuvant arthritis

Background Cryptotanshinone （CT） was originally isolated from the dried roots of Salvia militorrhiza, an herb that is used extensively in Asian medicine and the extracts of this herb have been used in the treatment of several pathologies, including cardiovascular diseases, hematological abnormalities, hepatitis, and hyperlipidemia, but no studies had been carried on the treatment for rheumatic diseases with it. This study aimed to investigate the effects of cryptotanshinone on immune functions in rats with adjuvant arthritis （AA）. Methods Complete Freund＇s adjuvant was used to induce AA in rats. Thymus and spleen was aseptically taken from normal rats and the AA rats. Then a thymus lymphoid cell suspension, splenic lymphoid cell suspension and peritoneal macrophage cell suspension were prepared. After adding CT （0.1 ug/ml, 1.0 ug/ml, 10 ug/ml, 100 ug/ml, 1000 ug/ml） into the suspension, T and B lymphocytes proliferation was determined by 3-（4,5-2 dimethylthiazal-2yl）2,5- diphenyltetrazoliumbromide （MTT） assay. And the activities of interleukin-1 （IL-1） and IL-2 were measured by the mouse lymphocytes proliferation assay. Results Thymic T and splenic B lymphocyte proliferation of the AA rat was significantly lower, and could be stored through using CT in vitro. CT （100ug/ml and 1000ug/ml） increased T or B lymphocytes proliferation in vitro （P 〈0.01）. In AA rats, the levels of IL-1 released by abdominal PMφ significantly increased whereas the level of IL-2 released by T cells decreased in vitro. CT （1000 pg/ml） decreased the production of IL-1 and promoted production of IL-2 in vitro （P 〈0.05）. Conclusions CT can ameliorate the abnormal immunological functions in AA rats.

美洛昔康凝胶治疗AA大鼠的疗效与局部药物浓度的关系研究

目的 探讨美洛昔康凝胶 (MelG)对佐剂性关节炎(AA)大鼠的治疗作用以及治疗效果与MelG血药浓度和局部药物浓度之间的关系。方法 采用FCA诱导大鼠AA ,用Volumemeter测定大鼠足爪容积 ,用HPLC测定MelG的血药浓度和足爪局部药物浓度 ,将足爪肿胀度与MelG的血药浓度或局部药物浓度作相关分析。结果 MelG明显抑制AA大鼠继发性炎症 ,MelG组大鼠血药浓度低于Mel组 ,足爪局部药物浓度则明显高于Mel组 ;AA大鼠继发性足爪肿胀度与MelG的足爪局部药物浓度间呈显著负相关。结论 MelG对AA大鼠的治疗作用与其足爪局部药物浓度高有关。

益气活血法对AA大鼠滑膜细胞凋亡及血清细胞因子的影响

目的选用黄芪注射液与川芎嗪注射液配伍,观察"益气活血法"对佐剂性关节炎(AA)大鼠滑膜细胞凋亡及血清细胞因子的影响。方法建立AA大鼠模型,设正常对照组、模型对照组、黄芪组、川芎嗪组、黄芪与川芎嗪配伍组和甲氨喋呤组进行对比观察,用药3周后检测各组大鼠足跖肿胀程度,滑膜细胞凋亡及血清细胞因子浓度。结果黄芪与川芎嗪配伍组能显著抑制注射足跖肿胀程度(P<0.05),显著提高滑膜细胞的凋亡率(P<0.01),并降低大鼠血清中IL-1、TNF-α、IL-6的水平(均P<0.01),上调IL-4的浓度(P<0.01),与甲氨喋呤组效果相当,甚至优于甲氨喋呤组。结论益气活血法可能通过促进AA大鼠滑膜细胞的凋亡,抑制某些炎性因子的释放,起到治疗类风湿性关节炎(RA)的作用。

关节炎慢性吗啡耐受大鼠脊髓背角EAAC1的表达(英文)

目的在佐剂性关节炎大鼠模型基础上建立关节炎大鼠慢性吗啡耐受模型,观察兴奋性氨基酸载体1(EAAC1)在关节炎慢性吗啡耐受大鼠的脊髓背角表达的变化,探讨EAAC1在吗啡耐受形成机制中的作用。方法将36只健康雄性SD大鼠随机分为6组(n=6),行鞘内置管。其中4组制成佐剂性关节炎模型,分别经鞘内给予生理盐水(A组)、吗啡10μg(B组)、吗啡20μg(C组)、吗啡10μg加纳洛酮10μg(D组),另外两组非致炎大鼠分别经鞘内给予生理盐水(E组)、吗啡20μg(F组)。各组给药均为1日2次,连续7d。用VonFrey丝动态检测大鼠50%机械缩爪阈值,分别以免疫组化和Westernblot方法检测各组大鼠给予吗啡后脊髓背角EAAC1表达。结果B、C两组关节炎大鼠在鞘内给予吗啡后第7天形成较稳定的机械痛敏,标志关节炎大鼠慢性吗啡耐受模型建立成功,其脊髓背角EAAC1的表达下调。结论脊髓EAAC1可能参与炎性痛大鼠慢性吗啡耐受的形成机制。

牛磺鹅去氧胆酸对AA大鼠下丘脑-垂体-肾上腺轴分泌激素的影响

旨在探讨牛磺鹅去氧胆酸(taurochenodeoxycholic acid,TCDCA)对佐剂性关节炎(AA)大鼠下丘脑-垂体-肾上腺轴(HPA)轴的影响。试验采用完全弗氏佐剂(CFA)法制备了AA大鼠,并将其分为5组,即模型组、TCDCA低剂量(0.1 g/kg)预防组和治疗组、TCDCA高剂量(0.2 g/kg)预防组和治疗组,并以正常的Wistar大鼠作为对照组,每组8只。给药方案:预防组于致炎前3 d,按照相应的药物剂量进行灌胃给药,1次/d,连续31 d。治疗组于致炎后第14天,按照相应的药物剂量进行灌胃给药,1次/d,连续14 d。通过外观观察对AA大鼠模型进行评价,并采用ELISA法检测大鼠血清中促肾上腺皮质激素释放激素(CRH)、促肾上腺皮质激素(ACTH)和皮质醇(CORT)的含量。结果显示:与正常组大鼠相比较,造模后14 d左右,模型组大鼠对侧足跖肿胀,造模后18~21 d,模型组大鼠尾部出现明显结节,提示AA大鼠模型制备成功。与正常对照组相比,模型组大鼠血清中ACTH、CORT含量显著升高(P<0.05),TCDCA低剂量治疗组可显著提高AA大鼠血清中CRH含量(P<0.05),预防用药或治疗用药后,TCDCA低、高剂量组均能显著或极显著提高AA大鼠血清中ACTH、CORT含量(P<0.05,P<0.01)。与模型组相比,TCDCA低剂量治疗组能显著提高AA大鼠血清中ACTH含量(P<0.05),TCDCA低剂量预防组和治疗组、高剂量治疗组均能提高AA大鼠血清中CRH含量,TCDCA低剂量预防组能提高AA大鼠血清中ACTH、CORT含量,而TCDCA高剂量预防组和治疗组均能降低AA大鼠血清中ACTH、CORT含量,但差异均不显著(P>0.05)。研究表明,TCDCA能调节机体HPA轴分泌CRH、ACTH、CORT水平,该结果可为进一步研究TCDCA抗炎免疫调节分子机制提供参考依据。

藏药五味甘露药浴颗粒对AA模型大鼠的药效作用研究

目的:观察藏药五味甘露药浴颗粒对AA模型大鼠的药效作用。方法:除正常组大鼠外,采用弗氏完全佐剂造模,随机分为模型组、地塞米松组(0. 150 mg/kg)、五味甘露药浴颗粒高剂量组(11. 80 g/L)、中剂量组(5. 90 g/L)、低剂量组(2. 95 g/L),药浴组40℃药水浴30 min/d,模型组和正常组40℃温水浴30 min/d。给药四周,采用致炎后足容积测量、测定大鼠胸腺、脾脏脏器系数、HE染色法观察致炎侧足踝关节滑膜病理改变,评价藏药五味甘露药浴颗粒对AA大鼠的治疗效果。结果:与模型组比较,五味甘露药浴颗粒高剂量组、中剂量组、低剂量组AA大鼠原发性足肿胀度均有减轻,滑膜细胞增生、炎细胞浸润、关节软骨破坏均有不同程度的改善。结论:经藏药浴五味甘露药浴颗粒治疗后,原发性足肿胀有明显下降的趋势,踝关节病理形态有所改善,大鼠的胸腺、脾脏系数有升高的趋势,提示该药可以明显减轻关节炎症程度,增强机体的免疫功能,对类风湿关节具有抗炎消肿的治疗作用。

基于miR-486-5p、巨噬细胞极化探讨新风胶囊改善佐剂性关节炎大鼠关节炎症的作用机制

目的:观察新风胶囊对佐剂性关节炎(adjuvant arthritis,AA)大鼠巨噬细胞极化及miR-486-5p的影响,探究新风胶囊改善AA大鼠炎症的可能机制。方法:32只SD大鼠被随机分为两个不同组别:正常组(NC组,8只)和造模组(24只),通过对造模组大鼠右后足跖皮内注射弗氏完全佐剂,成功构建AA大鼠模型后,进一步随机细分为3组:模型组(MC组,8只)、新风胶囊组(XFC组,8只)以及miR-486-5p抑制剂组(miR-486-5p inhibitor组,8只)。于炎症诱导后的第14天开始给予相应药物和试剂,NC组及MC组持续21天灌胃等量的生理盐水,然后检测各组AA大鼠足跖肿胀度(E)、关节炎指数(AI);采用酶联免疫吸附法测定(ELISA)检测大鼠细胞因子白细胞介素-23(interleukin-23,IL-23)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、IL-4及IL-13;流式细胞术检测大鼠外周血CD86、CD206表达;RT-qPCR检测大鼠关节滑膜miR-486-5p表达。结果:(1)与NC组比较,MC组大鼠E、AI及IL-23、TNF-α、CD86和miR-486-5p表达升高(P<0.01);IL-4、IL-13、CD206表达降低(P<0.01)。(2)与MC组比较,XFC组和miR-486-5p inhibitor组E、AI、IL-23、TNF-α、CD86和miR-486-5p表达降低(P<0.01);IL-4、IL-13、CD206表达升高(P<0.01)。(3)与miR-486-5p inhibitor组比较,XFC组IL-4、IL-13、CD206表达升高(P<0.01);IL-23、TNF-α、CD86和miR-486-5p表达降低(P<0.01);E、AI差异不明显(P>0.05)。结论:AA大鼠炎症与巨噬细胞极化和miR-486-5p表达有关,新风胶囊能明显改善AA大鼠关节炎症,其机制可能与抑制miR-486-5p表达,调节巨噬细胞极化有关。

佐剂性关节炎大鼠肾功能损伤及对肾脏有机阴离子转运体3表达的影响

目的研究佐剂性关节炎(AA)对大鼠肾脏功能和肾脏有机阴离子转运体3(OAT3)表达的影响。方法在大鼠右侧后足趾皮内注射弗氏完全佐剂建立AA模型。固定时间点收集大鼠血液和尿液,观察肾损伤指标的动态变化。通过肾脏的HE染色切片观察肾损伤情况。免疫组化和Western blot检测肾皮质OAT3蛋白和炎性因子白介素-6(IL-6)、白介素-1β(IL-1β)及肿瘤坏死因子-α(TNF-α)的表达水平。结果与正常组相比,AA大鼠关节炎指数评分、继发性足爪肿胀、关节肿胀数、全身评分均显著增加(P<0.05)。X线检测结果显示,AA模型大鼠足爪骨关节出现软组织水肿以及骨畸形的特征。生化指标显示,AA大鼠的肾损伤指标出现明显异常。免疫组化与Western blot结果显示,AA大鼠肾皮质OAT3的表达水平显著降低,同时炎性因子IL-6、TNF-α和IL-1β的表达水平明显升高(P<0.05)。结论AA大鼠的肾功能损伤可能与肾脏OAT3的表达降低及炎性因子表达升高有关。

程氏蠲痹汤对佐剂性关节炎大鼠体内氧化应激的影响

为探究程氏蠲痹汤(Cheng Shi Juan Bi Tang,CSJBT)对佐剂性关节炎(adjuvant arthritis,AA)大鼠体内氧化应激的影响,文章选用弗氏完全佐剂(complete Freund’s adjuvant,CFA)诱导SD大鼠建立关节炎模型。动物随机分为正常组、模型组、雷公藤多苷组(glycosides of Tripterygium wilfordii,GTW)(剂量0.01 g/kg)和CSJBT组(剂量分别为2.55、5.10、10.20 g/kg)。大鼠在造模后的第15天灌胃给药,连续治疗2周后,考察CSJBT对AA大鼠足爪肿胀度、体质量及氧化应激相关指标的影响,并采用HE染色法观察其对AA大鼠关节组织病理学变化的影响。结果显示CSJBT可缓解AA大鼠的关节肿胀,增加其体质量,改善关节滑膜组织病理学变化,减少滑膜组织中活性氧(reactive oxygen species,ROS)的产生以及血清中诱导型一氧化氮合酶(inducible nitric oxide synthase,iNOS)和丙二醛(MDA)的水平,而升高超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)及谷胱甘肽(GSH)的水平。结果表明CSJBT对AA大鼠体内的氧化应激反应有较好的抑制作用。

大鼠佐剂性关节炎模型表现特征及评价指标

目的:描述大鼠佐剂性关节炎(Adjuvant-induced arthritis,AA)临床表现主要指标的评价方法。方法:完全弗氏佐剂免疫SD大鼠诱导AA模型,进行全身及关节炎指数评分、计算关节肿胀数、测定足爪肿胀度、进行足爪X线摄片、踝关节病理检查及评分。结果:大鼠免疫后11天(d11)出现足爪红肿,全身及关节炎指数评分和关节肿胀数增加。炎症高峰期在免疫后d19～d28。大鼠出现活动障碍,可见耳朵、鼻和尾根部"关节炎"结节。X线可见关节软组织肿胀、骨密度降低、骨侵蚀病灶以及关节间隙狭窄甚至消失。踝关节病理见滑膜组织增生,炎细胞浸润,血管翳出现等。结论:大鼠AA是兼有局部关节炎症和全身表现的类风湿性关节炎动物模型。关节炎指数、关节肿胀数、足爪肿胀度、足爪X线摄片、踝关节病理等是评价AA模型临床表现的主要指标。

雷公藤多甙对大鼠佐剂性关节炎治疗作用和免疫机制的研究

利用大鼠佐剂性关节炎（ＡＡ）动物模型，研究了雷公藤多甙的治疗作用及其免疫机制。结果发现，雷公藤多甙能改善ＡＡ病情。雷公藤多甙治疗显效后，ＡＡ大鼠血清ＩＬ－１和ＩＬ－６水平明显降低，关节液内炎症细胞因子的水平下降，腹腔巨噬细胞及脾淋巴细胞分泌细胞因子的能力显著受到抑制。ＡＡ大鼠脾细胞增殖能力显著降低，雷公藤多甙治疗后对脾细胞增殖与ＡＡ对照组比较无明显差异。结果表明雷公藤多甙抗炎作用的发挥是通过免疫抑制作用而实现的。

佐剂性关节炎大鼠免疫功能的实验研究

目的 :阐明佐剂性关节炎 (AA)大鼠模型免疫病理特征及发病机理。方法 :应用弗氏完全佐剂诱发大鼠产生踝关节佐剂性关节炎 ,研究其免疫功能变化。结果 :AA大鼠致敏侧及对侧后肢踝关节均发生明显炎性肿胀 ,其脾细胞对SRBC诱导的抗体生成反应明显增强 ,但其脾细胞对PWM诱导的IgG的产生及血清类风湿因子 (RF)水平与对照组相比并无升高。AA大鼠对ConA诱导的脾细胞增殖反应较对照组显著降低 ,脾细胞抑制活性降低 ,CD8+ 比例较对照组增高 ,模型大鼠脾细胞IL 2mRNA表达水平较对照组明显增高 ,IFN γ ,IL 10及IL 4mRNA表达水平则明显降低。结论 :细胞免疫功能紊乱 ,尤其是Th1和Th2、Th和Ts之间的平衡紊乱是AA大鼠免疫功能异常的重要特征之一。