Spinal cord injury（SCI）-induced bone loss represents the most severe osteoporosis with no effective treatment.Past animal studies have focused primarily on long bones at the acute stage using adolescent rodents. To ...Spinal cord injury（SCI）-induced bone loss represents the most severe osteoporosis with no effective treatment.Past animal studies have focused primarily on long bones at the acute stage using adolescent rodents. To mimic chronic SCI in human patients, we performed a comprehensive analysis of long-term structural and mechanical changes in axial and appendicular bones in adult rats after SCI. In this experiment, 4-month-old Fischer 344 male rats received a clinically relevant T13 contusion injury. Sixteen weeks later, sublesional femurs, tibiae,and L4 vertebrae, supralesional humeri, and blood were collected from these rats and additional non-surgery rats for micro-computed tomography（m CT）, micro-finite element, histology, and serum biochemical analyses.At trabecular sites, extreme losses of bone structure and mechanical competence were detected in the metaphysis of sublesional long bones after SCI, while the subchondral part of the same bones showed much milder damage. Marked reductions in bone mass and strength were also observed in sublesional L4 vertebrae but not in supralesional humeri. At cortical sites, SCI induced structural and strength damage in both sub- and supralesional long bones. These changes were accompanied by diminished osteoblast number and activity and increased osteoclast number and activity. Taken together, our study revealed site-specific effects of SCI on bone and demonstrated sustained inhibition of bone formation and elevation of bone resorption at the chronic stage of SCI.展开更多
To examine the effect of myogenin gene on the differentiation of bovine skeletal muscle satellite cell, we constructed small interfering RNA plasmid vector to obtain myogenin knockdown bovine skeletal muscle cells, th...To examine the effect of myogenin gene on the differentiation of bovine skeletal muscle satellite cell, we constructed small interfering RNA plasmid vector to obtain myogenin knockdown bovine skeletal muscle cells, then used cell transfection, real time RCR and Western Blot to detect the influence of myogenin to cell differentiation. Results showed that the knockdown of myogenin significantly decreased its expression and other muscle-specific genes. Compared to the control, it could differentiate into few myotubes when challenged by low serum in the medium. These findings provided an important theoretical basis for further explore of the genetic mechanism in adult skeletal muscle, the remedy of muscle injuries and the cultivation of high-yield transgenic cattle.展开更多
Objective: The purpose of this study was to compare long-term stability and satisfaction between orthodontic camouflage and orthognathic surgery in treatment of moderate skeletal Class III adults. Materials and Method...Objective: The purpose of this study was to compare long-term stability and satisfaction between orthodontic camouflage and orthognathic surgery in treatment of moderate skeletal Class III adults. Materials and Methods: A total of 25 adults females who had been treated with orthodontic camouflage for Class III malocclusions were recalled at least 3 years post-treatment to evaluate stability and satisfaction with treatment outcomes. The data were compared with similar data for long-term outcomes in 21 patients with the same Class III problems who had bimaxillary surgical correction. Results: In the camouflage patients, small mean changes in skeletal landmark positions occurred over the long term, although the changes were generally much smaller than in the surgery patients. Dental changes in the surgery group were more severe than those in the camouflage group. The camouflage patients reported fewer functional or temporomandibular joint problems than did the surgery patients. Both groups reported similar levels of overall satisfaction with treatment. Conclusion: The results suggest that both camouflage and surgical treatment in moderate skeletal Class III adults can achieve satisfactory outcomes and provide long-term stability. If patients do not readily accept surgery because of potential surgical complications or financial difficulties, camouflage treatment may be an effective alternative treatment.展开更多
25 k D蛋白是一种新的与重症肌无力相关的蛋白质。为了检测该蛋白在人体不同组织中的表达并明确其在人骨骼肌中的亚细胞定位。本研究提取和纯化 2 5 k D蛋白 ,免疫小鼠制备了 2 5 k D蛋白的抗体 ;用免疫印迹方法验证了抗体的特异性并检...25 k D蛋白是一种新的与重症肌无力相关的蛋白质。为了检测该蛋白在人体不同组织中的表达并明确其在人骨骼肌中的亚细胞定位。本研究提取和纯化 2 5 k D蛋白 ,免疫小鼠制备了 2 5 k D蛋白的抗体 ;用免疫印迹方法验证了抗体的特异性并检测2 5 k D蛋白在成年人及胎儿 8种不同组织中的表达 ;用免疫组化和免疫电镜方法明确了 2 5 k D蛋白在骨骼肌中的细胞及亚细胞定位。结果显示 ,2 5 k D蛋白抗体只与肌肉匀浆样品中 2 5 k D蛋白特异性反应。在成年人及胎儿 8种不同组织中 ,2 5 k D蛋白只见于骨骼肌。免疫组化可见在肌膜下和横纹上有不均匀的免疫染色 ;免疫电镜显示在肌膜下有电子致密物沉积 ,肌丝中未见有电子致密物沉积。这些结果表明 :2 5 k D蛋白抗体具有很高的特异性 ,2 5 k D蛋白只表达于人骨骼肌中 ,其亚细胞定位在骨骼肌的肌膜下。展开更多
基金supported by the National Institutes of Health(R01DK095803 to LQ, 1K08HD049598 to YZ)Penn Center for Musculoskeletal Disorders P30AR050950(NIAMS/NIH)+1 种基金ASBMR Junior Faculty Osteoporosis Basic Research Award(to LQ)NIH/NIAMS R03-AR065145(to XSL)
文摘Spinal cord injury（SCI）-induced bone loss represents the most severe osteoporosis with no effective treatment.Past animal studies have focused primarily on long bones at the acute stage using adolescent rodents. To mimic chronic SCI in human patients, we performed a comprehensive analysis of long-term structural and mechanical changes in axial and appendicular bones in adult rats after SCI. In this experiment, 4-month-old Fischer 344 male rats received a clinically relevant T13 contusion injury. Sixteen weeks later, sublesional femurs, tibiae,and L4 vertebrae, supralesional humeri, and blood were collected from these rats and additional non-surgery rats for micro-computed tomography（m CT）, micro-finite element, histology, and serum biochemical analyses.At trabecular sites, extreme losses of bone structure and mechanical competence were detected in the metaphysis of sublesional long bones after SCI, while the subchondral part of the same bones showed much milder damage. Marked reductions in bone mass and strength were also observed in sublesional L4 vertebrae but not in supralesional humeri. At cortical sites, SCI induced structural and strength damage in both sub- and supralesional long bones. These changes were accompanied by diminished osteoblast number and activity and increased osteoclast number and activity. Taken together, our study revealed site-specific effects of SCI on bone and demonstrated sustained inhibition of bone formation and elevation of bone resorption at the chronic stage of SCI.
基金Supported by the Ministry of Agricultural Nuarture of New Varieties Genetically Modified Organisms Significant Special Funding (2008ZX08007-002)
文摘To examine the effect of myogenin gene on the differentiation of bovine skeletal muscle satellite cell, we constructed small interfering RNA plasmid vector to obtain myogenin knockdown bovine skeletal muscle cells, then used cell transfection, real time RCR and Western Blot to detect the influence of myogenin to cell differentiation. Results showed that the knockdown of myogenin significantly decreased its expression and other muscle-specific genes. Compared to the control, it could differentiate into few myotubes when challenged by low serum in the medium. These findings provided an important theoretical basis for further explore of the genetic mechanism in adult skeletal muscle, the remedy of muscle injuries and the cultivation of high-yield transgenic cattle.
文摘Objective: The purpose of this study was to compare long-term stability and satisfaction between orthodontic camouflage and orthognathic surgery in treatment of moderate skeletal Class III adults. Materials and Methods: A total of 25 adults females who had been treated with orthodontic camouflage for Class III malocclusions were recalled at least 3 years post-treatment to evaluate stability and satisfaction with treatment outcomes. The data were compared with similar data for long-term outcomes in 21 patients with the same Class III problems who had bimaxillary surgical correction. Results: In the camouflage patients, small mean changes in skeletal landmark positions occurred over the long term, although the changes were generally much smaller than in the surgery patients. Dental changes in the surgery group were more severe than those in the camouflage group. The camouflage patients reported fewer functional or temporomandibular joint problems than did the surgery patients. Both groups reported similar levels of overall satisfaction with treatment. Conclusion: The results suggest that both camouflage and surgical treatment in moderate skeletal Class III adults can achieve satisfactory outcomes and provide long-term stability. If patients do not readily accept surgery because of potential surgical complications or financial difficulties, camouflage treatment may be an effective alternative treatment.
文摘25 k D蛋白是一种新的与重症肌无力相关的蛋白质。为了检测该蛋白在人体不同组织中的表达并明确其在人骨骼肌中的亚细胞定位。本研究提取和纯化 2 5 k D蛋白 ,免疫小鼠制备了 2 5 k D蛋白的抗体 ;用免疫印迹方法验证了抗体的特异性并检测2 5 k D蛋白在成年人及胎儿 8种不同组织中的表达 ;用免疫组化和免疫电镜方法明确了 2 5 k D蛋白在骨骼肌中的细胞及亚细胞定位。结果显示 ,2 5 k D蛋白抗体只与肌肉匀浆样品中 2 5 k D蛋白特异性反应。在成年人及胎儿 8种不同组织中 ,2 5 k D蛋白只见于骨骼肌。免疫组化可见在肌膜下和横纹上有不均匀的免疫染色 ;免疫电镜显示在肌膜下有电子致密物沉积 ,肌丝中未见有电子致密物沉积。这些结果表明 :2 5 k D蛋白抗体具有很高的特异性 ,2 5 k D蛋白只表达于人骨骼肌中 ,其亚细胞定位在骨骼肌的肌膜下。
