Transplantation of adult spinal cord grafts into spinal cord transected rats improves their locomotor function

Grafted embryonic central neural tissue pieces can recover function of hemisected spinal cord in neonatal rats and promote axonal growth in adults. However, spinal cord segments from adults have not been used as donor segments for allogeneic transplantation. Here, we utilized adult spinal cord tissue grafts(aSCGs) as donor constructs for repairing complete spinal cord injury(SCI). Moreover, to provide a favourable microenvironment for SCI treatment, a growth factor cocktail containing three growth factors(brain-derived neurotrophic factor, neurotrophin-3 and vascular endothelial growth factor), was applied to the aSCG transplants. We found that the locomotor function was significantly improved 12 weeks after transplantation of aSCGs into the spinal cord lesion site in adult rats. Transplantation of aSCGs combined with these growth factors enhanced neuron and oligodendrocyte survival and functional restoration. These encouraging results indicate that treatment of complete SCI by transplanting aSCGs, especially in the presence of growth factors, has a positive effect on motor functional recovery, and therefore could be considered as a possible therapeutic strategy for SCI.

成人自发性椎管内出血的临床分析

目的探讨成人自发性椎管内出血的临床特点、治疗方案及预后。方法回顾性分析2012年1月至2021年5月遵义医科大学第一附属医院、第三附属医院脊柱外科35例自发性椎管内出血患者的临床症状、体征、影像学资料、出血原因、治疗方式及随访情况等。结果35例患者中硬脊膜外出血30例,髓内出血5例。血肿位于颈椎10例、胸椎14例、腰椎3例,颈胸椎3例,胸腰椎5例。采取手术治疗23例、保守治疗9例、拒绝手术3例。所有病例均随访,末次随访时神经功能改善率为87.5%。术前美国脊髓损伤协会(ASIA)分级为A级的8例患者术后改善率为50%。结论ASIA分级A~C级建议急诊行减压治疗,神经损伤轻或进行性好转者可保守治疗。干预时神经功能障碍程度越重,预后越差。