Aflatoxins( AFs) are a major pollution source of grain pollution and are secondary metabolites produced by Aspergillus flavus and A.parasiticus,which are one of the most toxic and carcinogenic substances.Feeding anima...Aflatoxins( AFs) are a major pollution source of grain pollution and are secondary metabolites produced by Aspergillus flavus and A.parasiticus,which are one of the most toxic and carcinogenic substances.Feeding animals with aflatoxin-contaminated food can cause toxicosis,acute liver injury or liver cancer in animals,and also can cause multiple organ damage,decline in production performance and disease resistance,even death,which brings great economic losses to animal husbandry.In addition,AFs can do great harm to the human body.When the body ingests large amounts of AFs,it may suffer from acute poisoning and acute liver damage; and AFs can cause chronic poisoning of the body with continuous intake of trace AFs.This paper briefly analyzed the effect of AFs on the structure and function of poultry immune organs,immune gene expression,immunosuppression and so on.展开更多
Objective:The aim of this study was to observe the affection of targeted therapy to plasmid AF-pGL3-hTERT-TK in HCC cell line HepG2.Methods:We constructed therapeutic plasmid pGL3-hTERT-TK(containing suicide gene TK t...Objective:The aim of this study was to observe the affection of targeted therapy to plasmid AF-pGL3-hTERT-TK in HCC cell line HepG2.Methods:We constructed therapeutic plasmid pGL3-hTERT-TK(containing suicide gene TK that promoted by promoter of hTERT) and was conjugated with AF-liposome(a protein that can combine with the receptor ASPGR on HCC cell surface).Then we transfected HCC cell line HepG2 and hepatic cell L02 with AF-pGL3-hTERT-TK,observed the effects of therapeutic plasmid AF-pGL3-hTERT-TK for HCC cell line growth and apoptosis in vitro by Flow cytometry and Tunnel method.Results:Our results showed that TK gene was 1100 bp in plasmid pGL3-hTERT-TK.Plasmid pGL3-hTERT-TK can effectively transfect HCC cell HepG2 and the transfection rate was 8.91%.By recognizing and combining effects of receptor protein ASPGR on HCC cell surface the therapeutic plasmid AF-pGL3-hTERT-TK could easily enter into HCC cell HepG2 and induce its apoptosis.The apoptosis rate was 85.87% while only 8.65% in L02 cell.Four days after AF-pGL3-hTERT-TK transfected HepG2 was intervention by ganciclovir(GCV),a lot of apoptotic bodies were found by Tunnel analysis,while little apoptotic body was found in hepatic cell L02.Conclusion:AF-pGL3-hTERT-TK can target to HCC cell line and induce it to apoptosis,almost has no influence on hepatic cell L02.AF-pGL3-hTERT-TK has the potential therapeutic effects for HCC.展开更多
基金Supported by Program for International Cooperation of Ministry of Science and Technology(2011DFA30760)
文摘Aflatoxins( AFs) are a major pollution source of grain pollution and are secondary metabolites produced by Aspergillus flavus and A.parasiticus,which are one of the most toxic and carcinogenic substances.Feeding animals with aflatoxin-contaminated food can cause toxicosis,acute liver injury or liver cancer in animals,and also can cause multiple organ damage,decline in production performance and disease resistance,even death,which brings great economic losses to animal husbandry.In addition,AFs can do great harm to the human body.When the body ingests large amounts of AFs,it may suffer from acute poisoning and acute liver damage; and AFs can cause chronic poisoning of the body with continuous intake of trace AFs.This paper briefly analyzed the effect of AFs on the structure and function of poultry immune organs,immune gene expression,immunosuppression and so on.
基金Supported by a grant from the National Natural Sciences Foundation of China (No. 30672405)
文摘Objective:The aim of this study was to observe the affection of targeted therapy to plasmid AF-pGL3-hTERT-TK in HCC cell line HepG2.Methods:We constructed therapeutic plasmid pGL3-hTERT-TK(containing suicide gene TK that promoted by promoter of hTERT) and was conjugated with AF-liposome(a protein that can combine with the receptor ASPGR on HCC cell surface).Then we transfected HCC cell line HepG2 and hepatic cell L02 with AF-pGL3-hTERT-TK,observed the effects of therapeutic plasmid AF-pGL3-hTERT-TK for HCC cell line growth and apoptosis in vitro by Flow cytometry and Tunnel method.Results:Our results showed that TK gene was 1100 bp in plasmid pGL3-hTERT-TK.Plasmid pGL3-hTERT-TK can effectively transfect HCC cell HepG2 and the transfection rate was 8.91%.By recognizing and combining effects of receptor protein ASPGR on HCC cell surface the therapeutic plasmid AF-pGL3-hTERT-TK could easily enter into HCC cell HepG2 and induce its apoptosis.The apoptosis rate was 85.87% while only 8.65% in L02 cell.Four days after AF-pGL3-hTERT-TK transfected HepG2 was intervention by ganciclovir(GCV),a lot of apoptotic bodies were found by Tunnel analysis,while little apoptotic body was found in hepatic cell L02.Conclusion:AF-pGL3-hTERT-TK can target to HCC cell line and induce it to apoptosis,almost has no influence on hepatic cell L02.AF-pGL3-hTERT-TK has the potential therapeutic effects for HCC.
