Strategies for Enhancing the Affinity of College Ideological and Political Course Teachers

This article aims to explore effective ways to enhance the affinity of ideological and political course teachers in universities.By analyzing the connotation of affinity,the factors that affect the affinity of ideological and political course teachers are analyzed,and corresponding improvement strategies are proposed.Research suggests that strengthening the construction of teacher ethics and conduct,improving teaching skills,enhancing emotional engagement,and enhancing practical training are key paths to enhance the affinity of ideological and political course teachers.The implementation of these paths will help improve the teaching quality and effectiveness of ideological and political courses,and promote the comprehensive development of students.

Local and global approaches of affinity propagation clustering for large scale data

Recently a new clustering algorithm called 'affinity propagation' (AP) has been proposed, which efficiently clustered sparsely related data by passing messages between data points. However, we want to cluster large scale data where the similarities are not sparse in many cases. This paper presents two variants of AP for grouping large scale data with a dense similarity matrix. The local approach is partition affinity propagation (PAP) and the global method is landmark affinity propagation (LAP). PAP passes messages in the subsets of data first and then merges them as the number of initial step of iterations; it can effectively reduce the number of iterations of clustering. LAP passes messages between the landmark data points first and then clusters non-landmark data points; it is a large global approximation method to speed up clustering. Experiments are conducted on many datasets, such as random data points, manifold subspaces, images of faces and Chinese calligraphy, and the results demonstrate that the two ap-proaches are feasible and practicable.

Dyes with high affinity for polylactide

Attempts were made to develop dyes with high affinity for polylactide as an alternative to the existent commercial disperse dyes. The dyes synthesized according to the affinity concept of dye to polylactide exhibited excellent dyeing properties on polylactide compared with the commercial disperse dyes.

A core–shell amidoxime electrospun nanofiber affinity membrane for rapid recovery Au(Ⅲ) from water

An affinity membrane was prepared by coaxial electrospinning and amidoxime(AONFA),and it was applied to selectively recovery Au(Ⅲ)from an aqueous solution.The static adsorption results showed that,when p H at 5,the maximum adsorption capacity of AONFA membrane for Au(Ⅲ)was 509.3 mg·g^(-1).AONFA membrane exhibit much higher affinity and selectivity towards Au(Ⅲ)than other metal cations.The membrane could be regenerated effectively by mixture solution of thiourea and HCl,and the desorption ratio reached almost 100%after 4 hours desorption.The dead-end filtration results showed that,the membrane utilization efficiency and adsorption capacity can be improved by increasing the flow rate,while increasing the concentration shorted the breakthrough process and had little impact to adsorption capacity.We can flexibly adjust the flow rate and concentration according to the situation to obtain the maximum utilization efficiency of the membrane in filtration process.The dynamic adsorption capacity is higher than the static adsorption capacity.The adsorption mechanism for Au(Ⅲ)is electrostatic adsorption and reduction.Thus,AONFA membrane filtration was demonstrated to be a promising method for continuous recover Au(Ⅲ)from wastewater.

A Novel Method for Studying the interaction of Macromolecule with Small Molecule by Means of Affinity Capillary Electrophoresis

A novel capillary electrophoresis method coupled with on-line microdialysis using an attachable electrode has been developed to study the interaction of macromolecule with small molecule. The binding constants of bovine serum album (BSA) with D,L-tryptophan (Trp), sulfamethoxazole (SMZ) with trypsin and chymotrypsin were determined. These values are 2.3 x 10(4)L/mol for BSA-L-Trp; 1.77 x 10(3) L/mol for BSA-D-Trp in pH 7.4, 50 mmol/ L phosphate; 1.4 x 10(4) L/mol for SMZ- trypsin and 6.0 x 10(3) L/mol for SMZ-chymotrypsin in pH 6.5, 25 mmol/L Tris buffer. The proposed method has merits of speed, low sample consumption and readily available to be performed in desired physiological conditions.

Accurate calculation of electron affinity for S_3

The accurate equilibrium structures of S_3 and S_3^- are determined by the coupled-cluster method with single, double excitation and perturbative triple excitation(CCSD(T)) with basis sets of aug-cc-pV(n+d)Z(n = T, Q, 5, or 6), complete basis set extrapolation functions with two-parameters and three-parameters, together with considering the contributions due to the core-valence electron correlation, scalar relativistic effects, spin–orbit coupling, and zero-point vibrational corrections. Our calculations show that both the neutral S_3 and anion S_3^- have open forms with C_(2r) vsymmetry. On the basis of the stable geometries, the adiabatic electron affinity of S_3 is determined to be 19041(11) cm^(-1), which is in excellent agreement with the experimental data(19059(7) cm^(-1)). The dependence of geometries and electron affinity on the computation level and physical corrections is discussed. The present computational results are helpful to the experimental molecular spectroscopy and bonding of S_3.

Preparation of Iminodiacetic Acid-Polyethylene Glycol for Immobilized Metal Ion Affinity Partitioning

The synthesis route was investigated and optimized for the preparation of iminodiacetic acid-polyethylene glycol (IDA-PEG) for immobilized metal ion affinity partitioning in aqueous two-phase systems. IDA-PEG was synthesized from PEG in two steps by the reaction of iminodiacetic acid with a monosubstituted derivative of epichlorohydrin-activated PEG. The Cu2+ content combined with IDA-PEG was determined by atomic absorption spectrometry as 0.5 mol·mol^-1 (PEG). Furthermore, the affinity partitioning behavior of lactate dehydrogenase in polyethylene glycol/hydroxypropyl starch aqueous two-phase systems was studied to clarify the affinity effect of the Cu(Ⅱ)-IDA-PEG.

Poly(vinylidene fluoride) Modified Commercial Paper as a Separator with Enhanced Thermal Stability and Electrolyte Affinity for Lithium-ion Battery

Commercial paper is of great potential as a ready-made substrate to make battery separator due to superior electrolyte affinity of cellulose.Nevertheless,the direct utilization of commercial paper as a separator is impracticable because of its micro-sized holes between coarse cellulose fibers,which might induce short circuits.Herein,a novel reinforced composite separator is proposed by modifying commercial paper(CP)with highdielectric polymer poly(vinylidene fluoride)(PVDF)via a vacuum filtration method.The paper substrate enables excellent electrolyte wettability and high ionic conductivity of the CP-PVDF composite separator due to the superior electrolyte affinity of cellulose molecule.Meanwhile,the strong hydrogen bonds between F atoms in PVDF and H atoms in the-OH groups of cellulose endow the separator with high thermal stability and mechanical strength.Moreover,the CP-PVDF exhibits outstanding interfacial compatibility toward Li metal anode and guarantees the prominent cycle durability of symmetric Li/Li cells up to 600 h.As a result,the LiFePO_(4)/Li cells assembled with CP-PVDF separator show dramatic rate performance with high discharge capacity of 113.7 m Ah g^(-1),and prolonged cycle life at 5 C.This work indicates that the paper-based composite membranes possess great potential for high-safety and electrochemical performance batteries.

Silica-supported Macroporous Chitosan Bead for Affinity Purification of Trypsin Inhibitor

Macroporous cross-linking chitosan layer coated on silica gel （CTS-SiO2） was prepared by phase inversion and polyethylene glycol （PEG）molecular imprinting methods. Formation of macroporous surtace was investigated by scanning electron microscopy （SEM） and BET analysis.The prepared bead was activated by reacting with 1,2-ethylene digiycidyl ether for introducing epoxy groups, and trypsin could be efficiently immobilized on the bead as a biospecific ligand.The bead bearing trypsin was employed to purify trypsin inhibitor （TIs） from egg white as affinity adsorbent.

Modification of Nylon Membrane Used for Affinity Adsorption

Nylon membrane was modified by binding with polyhydroxyl-containing materials to increase its hydrophilicity and reduce its nonspecific interaction with proteins. The effect of binding hydrophilic materials on amount of ligand bound-Cibacron Blue F3GA (CBF) was investigated. Experimental data showed that the amount of CBF bound can be increased significantly after binding of hydrophilic materials.

Design of Ligands for Affinity Purification of G-CSF Based on Peptide Ligands Derived from a Peptide Library

Combinatorial peptide libraries have become powerful tools to screen functional ligands by the principle of affinity selection. We screened in a phage peptide library to investigate potential peptide affinity ligands for the purification of human granulocyte colonystimulation factor(hGCSF). Peptide ligands will be promising to replace monoclonal antibodies as they have advantages of high stability, efficiency, selectivity and low price.

High Performance Affinity Chromatography of Antithrombin III Based on Monodisperse Poly (glycidyl methacrylate) Beads

A new approach for the separation of antithrombin III with high performance affinity chromatography (HPAC) was described. A novel monodisperse, non-porous, cross-linked poly (glycidyl methacrylate) beads (PGMA) were used as the affinity support. With the water-soluble carbodiimide, heparin was linked covalently to amino-PGMA-beads, which was prepared by amination of PGMA. The adsorbent obtained exhibits high binding activity to antithrombin III (ATIII), good resolution and excellent mechanical properties and can be used under high flow rate.

Boronic Acids as Ligands for Affinity Chromatography

A review on the principles and applications of boronic acids as affinity ligands for the chromatographic separation of carbohydrates,nucleic acid components,glycoproteins,and other small biomolecules.The mechanisms of interactions between boronate ligands and analytes are described.Various boronate ligands and supports are discussed.Examples of the use of boronate affinity chromatography for separation of each class of analytes are presented.

Preparation of Cibacron Blue F3GA Bonded Poly (styrenedivinylbenzene) (PSDVB) Microbeads Used for High Performance Affinity Chromatography

Rigid PSDVB microbeads have been modified with poly(vinyl alcohol) (PVA) adsorbed on their surface to produce an affinity medium. Then Cibacron Blue F3GA was covalently attached to the supports. The initial concentration of PVA has effect on the adsorption of PVA. The non-specific interaction of bovine serum albumin (BSA) on the microbeads decreases with the PVA adsorbed. The pH stability test shows that the affinity medium is stable up to pH 11.0. And it has specific interaction with lysozyme, but not with pepsin.

Expression of a Hybrid Human Superoxide Dismutase with a High Affinity for Heparin

A designed heparin-affinity of human Cu, Zn-SOD is described. The natural leader peptide of P.leiognathi Cu, Zn-SOD and a heparin-binding peptide containing a stretch of 7 Arg were fused to the N-terminal and the C-terminal of human Cu, Zn-SOD respectively. The resulted hybrid enzyme had not only a normal SOD activity but also a high affinity for heparin eluted on the heparin-Sepharose column at 0.4 mol/L NaCl. Some properties, such as the optimum pH, the thermostability and the half-life in the circulation of rats, were also analyzed.

Using of <i>In-Situ</i>Mercury Film Sensor Hyphenated with Affinity Voltammetry for High Throughput Drug-Protein Binding Studies

A new simple and reliable in-situ mercury film sensor coupled with affinity differential pulse stripping voltammetry (ADSPV) or affinity cyclic voltammetry (ACV) was investigated. The interaction of fenoprofen with bovine serum albumin (BSA) onto the proposed electrochemical sensor was studied. The nature of the electrochemical process of fenoprofen by cyclic voltammetry was depicted. Reproducibility of the proposed method was checked giving a precision of 0.073 standard deviation. The limit of detection and limit of quantification were 7.0 and 22.0 nmol/L, respectively. Fenoprofen was interacted with BSA by 1:1 stoichiometry to form electroinactive supramolecular complex. The binding constant was precisely estimated by non-linear regression analysis based on the shifting of analyte peak potentials. The proposed experiments and data analysis could be used to investigate the drug-protein binding constant within a short analysis time compared to other chromatographic techniques.

On the Accuracy of the Complete Basis Set Extrapolation for Anionic Systems: A Case Study of the Electron Affinity of Methane

Recent experimental evidence suggests again the existence of the metastable methane anion in plasma swarms. In order to test the reliability of the complete basis set (CBS) extrapolation scheme with augmented correlation-consistent basis sets for anionic molecules, we study the evolution of the electron affinity of methane with benchmark ab initio calculations with aug-cc basis sets up to aug-cc-pV6Z + diffuse. Geometry optimizations and vibrational analysis were done at the MP2 level. The electron affinity (EA) was calculated at the MP2 and CCSD(T) levels with and without frozen core and including the extrapolations to the CBS limit. Using the aug-cc-pVnZ basis sets it is found that two non-decreasing CCSD(T) CBS limits exist for the EA (0.29 and 0.53 eV) obtained with the n = 3, 4, 5 and n = 4, 5, 6 series, respectively. A new scheme is proposed which can be generalized for very accurate quantum chemical description of molecular anions: the standard aug-cc-pVnZ basis sets can be supplemented with extra-diffuse orbitals using a simple even-tempered scheme. This yields a reliable CBS extrapolation method to develop a (discrete approximation of a) continuum anionic state near ionization, viz., one that closely matches the energy of the corresponding neutral state. These results show that CH4 has no stable anions of 2A1 symmetry, implying that plasma swarms with anionic methane consist of metastable rather than stable methane anions.

Affinity ultrafiltration of DNA topoisomerases-targeted compounds determined with HPLC／ESI-MS for drug candidate screening

A method of screening assay is demonstrated. The approach is based on the affinity of antitumor candidates for topoisomerases. In this method, antitumor candidates are fished out using topoisomerases as targets. Traditional analysis of complex compounds typically encounters signal suppression due to the relatively low concentrations, but enzyme-affinity screening for the active compounds can effectively concentrate the desired analysts into a small volume of high concen-tration. Active compounds are separated from non-affinity compounds by ultrafiltration. The molecules-enzymes complexes that are retained on the filter are subsequently separated by acidification to obtain the topoisomerases-affinity compounds for analysis on High Performance Liquid Chromatography coupled with electrospray ionization mass spectrometric detec-tion (ESI-MS). This enzyme-affinity based screening assay provides a highly specific and efficient method that can directly screen, identify, and acquire drug candidates thus improving the accuracy and speed of high-throughput screening activities.

Virtual Machine Consolidation with Multi-Step Prediction and Affinity-Aware Technique for Energy-Efficient Cloud Data Centers

Virtual machine(VM)consolidation is an effective way to improve resource utilization and reduce energy consumption in cloud data centers.Most existing studies have considered VM consolidation as a bin-packing problem,but the current schemes commonly ignore the long-term relationship between VMs and hosts.In addition,there is a lack of long-term consideration for resource optimization in the VM consolidation,which results in unnecessary VM migration and increased energy consumption.To address these limitations,a VM consolidation method based on multi-step prediction and affinity-aware technique for energy-efficient cloud data centers(MPaAF-VMC)is proposed.The proposed method uses an improved linear regression prediction algorithm to predict the next-moment resource utilization of hosts and VMs,and obtains the stage demand of resources in the future period through multi-step prediction,which is realized by iterative prediction.Then,based on the multi-step prediction,an affinity model between the VM and host is designed using the first-order correlation coefficient and Euclidean distance.During the VM consolidation,the affinity value is used to select the migration VM and placement host.The proposed method is compared with the existing consolidation algorithms on the PlanetLab and Google cluster real workload data using the CloudSim simulation platform.Experimental results show that the proposed method can achieve significant improvement in reducing energy consumption,VM migration costs,and service level agreement(SLA)violations.

Stable titanium metal-organic framework with strong binding affinity for ethaneremoval

Direct separation of high purity ethylene(C_(2)H_(4))from an ethane(C_(2)H_(6))/ethylene mixture is a critical and challenging task owing to the very similar molecular size and physical properties of the two components.While some studies have attempted this separation,there is a lack of excellent porous materials with strong binding affinity for C_(2)H_(6)-selective adsorption via an energy-efficient adsorptive separation process.Herein,we report a titanium metal-organic framework with strong binding affinity and excellent stability for the highly efficient removal of C_(2)H_(6) from C_(2)H_(6)/C_(2)H_(4) mixtures.Single component adsorption isotherms demonstrated a larger amount of adsorbed ethane(1.16 mmol·g^(-1) under 1 kPa)and high C_(2)H_(6)/C_(2)H_(4) selectivity(2.7)for equimolar C_(2)H_(6)/C_(2)H_(4) mixtures,especially in the low-pressure range,which is further confirmed by the results of grand canonical Monte Carlo simulations for C_(2)H_(6) adsorption in this framework.The experimental breakthrough curves showed that C_(2)H_(4) with a high purity was collected directly from both 1:9 and 1:15 C_(2)H_(6)/C_(2)H_(4)(volume ratio)mixtures at 298 K and 100 kPa.Moreover,the unchanged adsorption and separation performance after cycling experiments confirmed the promising applicability of this material in future.