Peanuts are important oil and food sources worldwide and can be easily contaminated by highly toxic aflatoxins （AFs） , which often exist in peanuts and related foodstuff. In this experiment, a total of 12 A. flavu...Peanuts are important oil and food sources worldwide and can be easily contaminated by highly toxic aflatoxins （AFs） , which often exist in peanuts and related foodstuff. In this experiment, a total of 12 A. flavus strains were isolated from soil collected from 7 cities of China and the aflatoxin con-tamination levels in peanuts in these areas were detected to find out the relationship between aflatoxin contamination level and amount of soil isolates in the same region, as well as the correlation between cli-mate type and peanut aflatoxin contamination level. In this study, we found that in high - temperature and moist areas, toxin producing strains were easier to be isolated and the aflatoxin contamination level in peanuts was higher. We also studied the association between the aflR expression determined by fluorogen- ic quantitative PCR and the aflatoxin production. The results showed all 3 atoxigenic isolates had the same cycle threshold 21. 38, suggesting that atoxigenic strains had the same expression of aflR, which was the key gene of aflatoxin production.展开更多
文摘Peanuts are important oil and food sources worldwide and can be easily contaminated by highly toxic aflatoxins （AFs） , which often exist in peanuts and related foodstuff. In this experiment, a total of 12 A. flavus strains were isolated from soil collected from 7 cities of China and the aflatoxin con-tamination levels in peanuts in these areas were detected to find out the relationship between aflatoxin contamination level and amount of soil isolates in the same region, as well as the correlation between cli-mate type and peanut aflatoxin contamination level. In this study, we found that in high - temperature and moist areas, toxin producing strains were easier to be isolated and the aflatoxin contamination level in peanuts was higher. We also studied the association between the aflR expression determined by fluorogen- ic quantitative PCR and the aflatoxin production. The results showed all 3 atoxigenic isolates had the same cycle threshold 21. 38, suggesting that atoxigenic strains had the same expression of aflR, which was the key gene of aflatoxin production.
