Objective The initiation and progression of lung carcinomas are critically regulated by long non-coding RNAs(lncRNAs).However,the role of lncRNAs in the pathways causing lung cancer remains unknown.Methods Cell morpho...Objective The initiation and progression of lung carcinomas are critically regulated by long non-coding RNAs(lncRNAs).However,the role of lncRNAs in the pathways causing lung cancer remains unknown.Methods Cell morphology was regularly observed using an inverted phase-contrast microscope.Cell viability was assessed using CCK-8 according to the manufacturer’s instructions.Total RNA was retrotranscribed from each specimen using the RNAiso Plus Kit.The RT-PCR data were calculated using the Ct approach for comparison.Flow cytometric analyses were prepared by Click-iT™Plus TUNEL Assay for In Situ apoptosis detection,with Alexa Fluor^(TM)594 dye,as instructed.RNA immunoprecipitation assays were used to determine RNA concentration.Results Activated natural killer cells repeat and PH domain-containing protein 2 antisense RNA 1(AGAP2-AS1)levels in cancerous tissues were significantly correlated with cancerous tumor node metastasis(TNM)stage,with cancerous AGAP2-AS1 levels being higher in cancerous tissues than healthy tissues.Patients withelevated AGAP2-AS1 levels had considerably worse outcomes than those with reduced AGAP2-AS1 levels,regardless of the progression-free or overall survival.Functionally,AGAP2-AS1 downregulation represseslung cancer cell growth.AGAP2-AS1 elimination induces erastin-mediated ferroptosis in lung cancer cells.However,the ferritin inhibitor FERSINT-1 negated this result,whereas ERASTIN induced lung cancer cellmortality.After AGAP2-AS1 silencing,erastin-treated lung cancer cells showed a remarkable decrease inGSH levels.These results indicated that AGAP2-AS1 enhanced the stabilization of SLC7A11 mRNA via Recombinant Insulin Like Growth Factor Binding Protein 2(IGF BP2).Patients with elevated AGAP2-AS1 had considerably worse outcomes.Down-regulating AGAP2-AS1 was able to repress lung cancer cell growth and induce greater Erastin-mediated ferroptosis.Lungcancer cells treated with Erastin exhibited a remarkable decrease inglutathione(GSH)levels.The mechanical findingsindicated that AGAP2-AS1 enhanced the stabilization of SLC7A11 mRNA via the IGF2BP2.Conclusion We identified a novel effect of AGAP2-AS1 on TNM staging and the prognosis of patientswith lungcancer by modulating SLC7A11 mRNA stability and ferroptosis.展开更多
With the rapid development of air transportation in recent years,airport operations have attracted a lot of attention.Among them,airport gate assignment problem(AGAP)has become a research hotspot.However,the real-time...With the rapid development of air transportation in recent years,airport operations have attracted a lot of attention.Among them,airport gate assignment problem(AGAP)has become a research hotspot.However,the real-time AGAP algorithm is still an open issue.In this study,a deep reinforcement learning based AGAP(DRL-AGAP)is proposed.The optimization object is to maximize the rate of flights assigned to fixed gates.The real-time AGAP is modeled as a Markov decision process(MDP).The state space,action space,value and rewards have been defined.The DRL-AGAP algorithm is evaluated via simulation and it is compared with the flight pre-assignment results of the optimization software Gurobiand Greedy.Simulation results show that the performance of the proposed DRL-AGAP algorithm is close to that of pre-assignment obtained by the Gurobi optimization solver.Meanwhile,the real-time assignment ability is ensured by the proposed DRL-AGAP algorithm due to the dynamic modeling and lower complexity.展开更多
This paper will discuss Plato＇s view of love in The Symposium, in particular the arguments presented by the Diotima character, but not neglecting all the other views of love presented therein. The paper, as the title...This paper will discuss Plato＇s view of love in The Symposium, in particular the arguments presented by the Diotima character, but not neglecting all the other views of love presented therein. The paper, as the title indicates, will be confined to a comparison and evaluation of Platonic love against love as articulated within Christianity. Both forms of love will be analyzed and I will attempt to show that although Plato, through Socrates （and Socrates through the Diotima character）, tries to redeem the traditional understanding of love in the ancient Greek society that he was living in, Platonic love is still very different from the Christian concept of love.展开更多
Due to the top priority of the command to love God over the command to love one＇s neighbor, these two commands wil/ fall into a profound paradox ＂to violate generally accepted morality for the sake of Christian fait...Due to the top priority of the command to love God over the command to love one＇s neighbor, these two commands wil/ fall into a profound paradox ＂to violate generally accepted morality for the sake of Christian faith. ＂ The orthodox doctrine of ＂justification by faith＂ expresses this paradox in a most definite way, because it regards unbelief in God as the most unacceptable, hateful, and punishable sin against God. Only by giving up this priority can the two love commands truly move beyond the moral paradox and realize their essential and holy unity in the framework of a new ＂ theology of agape＂ or a new doctrine of ＂justification by agape. ＂展开更多
基金Supported by the Wuhan Municipal Health Commission Medical Research Project-Youth Project(No.WZ20Q04).
文摘Objective The initiation and progression of lung carcinomas are critically regulated by long non-coding RNAs(lncRNAs).However,the role of lncRNAs in the pathways causing lung cancer remains unknown.Methods Cell morphology was regularly observed using an inverted phase-contrast microscope.Cell viability was assessed using CCK-8 according to the manufacturer’s instructions.Total RNA was retrotranscribed from each specimen using the RNAiso Plus Kit.The RT-PCR data were calculated using the Ct approach for comparison.Flow cytometric analyses were prepared by Click-iT™Plus TUNEL Assay for In Situ apoptosis detection,with Alexa Fluor^(TM)594 dye,as instructed.RNA immunoprecipitation assays were used to determine RNA concentration.Results Activated natural killer cells repeat and PH domain-containing protein 2 antisense RNA 1(AGAP2-AS1)levels in cancerous tissues were significantly correlated with cancerous tumor node metastasis(TNM)stage,with cancerous AGAP2-AS1 levels being higher in cancerous tissues than healthy tissues.Patients withelevated AGAP2-AS1 levels had considerably worse outcomes than those with reduced AGAP2-AS1 levels,regardless of the progression-free or overall survival.Functionally,AGAP2-AS1 downregulation represseslung cancer cell growth.AGAP2-AS1 elimination induces erastin-mediated ferroptosis in lung cancer cells.However,the ferritin inhibitor FERSINT-1 negated this result,whereas ERASTIN induced lung cancer cellmortality.After AGAP2-AS1 silencing,erastin-treated lung cancer cells showed a remarkable decrease inGSH levels.These results indicated that AGAP2-AS1 enhanced the stabilization of SLC7A11 mRNA via Recombinant Insulin Like Growth Factor Binding Protein 2(IGF BP2).Patients with elevated AGAP2-AS1 had considerably worse outcomes.Down-regulating AGAP2-AS1 was able to repress lung cancer cell growth and induce greater Erastin-mediated ferroptosis.Lungcancer cells treated with Erastin exhibited a remarkable decrease inglutathione(GSH)levels.The mechanical findingsindicated that AGAP2-AS1 enhanced the stabilization of SLC7A11 mRNA via the IGF2BP2.Conclusion We identified a novel effect of AGAP2-AS1 on TNM staging and the prognosis of patientswith lungcancer by modulating SLC7A11 mRNA stability and ferroptosis.
基金Supported by the National Natural Science Foundation of China(No.U1633115)the Science and Technology Foundation of Beijing Municipal Commission of Education(No.KM201810005027)。
文摘With the rapid development of air transportation in recent years,airport operations have attracted a lot of attention.Among them,airport gate assignment problem(AGAP)has become a research hotspot.However,the real-time AGAP algorithm is still an open issue.In this study,a deep reinforcement learning based AGAP(DRL-AGAP)is proposed.The optimization object is to maximize the rate of flights assigned to fixed gates.The real-time AGAP is modeled as a Markov decision process(MDP).The state space,action space,value and rewards have been defined.The DRL-AGAP algorithm is evaluated via simulation and it is compared with the flight pre-assignment results of the optimization software Gurobiand Greedy.Simulation results show that the performance of the proposed DRL-AGAP algorithm is close to that of pre-assignment obtained by the Gurobi optimization solver.Meanwhile,the real-time assignment ability is ensured by the proposed DRL-AGAP algorithm due to the dynamic modeling and lower complexity.
文摘This paper will discuss Plato＇s view of love in The Symposium, in particular the arguments presented by the Diotima character, but not neglecting all the other views of love presented therein. The paper, as the title indicates, will be confined to a comparison and evaluation of Platonic love against love as articulated within Christianity. Both forms of love will be analyzed and I will attempt to show that although Plato, through Socrates （and Socrates through the Diotima character）, tries to redeem the traditional understanding of love in the ancient Greek society that he was living in, Platonic love is still very different from the Christian concept of love.
文摘Due to the top priority of the command to love God over the command to love one＇s neighbor, these two commands wil/ fall into a profound paradox ＂to violate generally accepted morality for the sake of Christian faith. ＂ The orthodox doctrine of ＂justification by faith＂ expresses this paradox in a most definite way, because it regards unbelief in God as the most unacceptable, hateful, and punishable sin against God. Only by giving up this priority can the two love commands truly move beyond the moral paradox and realize their essential and holy unity in the framework of a new ＂ theology of agape＂ or a new doctrine of ＂justification by agape. ＂
