从大连沿海的50份石花菜中筛选出相对酶活较高的83株产琼胶酶菌株(酶活力测定采用3,5-二硝基水杨酸法),挑选琼胶酶活力最高的菌株并通过形态学特征、生理生化特征及16S r RNA基因序列分析研究其分类地位。结果表明,该菌株(暂命名为ZGR-...从大连沿海的50份石花菜中筛选出相对酶活较高的83株产琼胶酶菌株(酶活力测定采用3,5-二硝基水杨酸法),挑选琼胶酶活力最高的菌株并通过形态学特征、生理生化特征及16S r RNA基因序列分析研究其分类地位。结果表明,该菌株(暂命名为ZGR-26)为革兰氏阴性杆菌,与食琼胶弧菌(Vibrio agarivorans)序列同源性达到99%,两者生理生化特性基本相符,可初步确定为食琼胶弧菌(Vibrio agarivorans)。筛选得到了新型的产琼胶酶海洋细菌——食琼胶弧菌(Vibrio agarivorans),分泌琼胶酶活力较高(32.1 U/m L),为微生物降解法生产琼胶寡糖提供了新的出发菌株。展开更多
Antibiotic resistance has emerged as a major global threat to human health. Among the strategies employed by pathogens to acquire resistance the use of metallo-β-lactamases (MBLs), a family of dinuclear metalloenzyme...Antibiotic resistance has emerged as a major global threat to human health. Among the strategies employed by pathogens to acquire resistance the use of metallo-β-lactamases (MBLs), a family of dinuclear metalloenzymes, is among the most potent. MBLs are subdivided into three groups (i.e. B1, B2 and B3) with most of the virulence factors belonging to the B1 group. The recent discovery of AIM-1, a B3-type MBL, however, has illustrated the potential health threat of this group of MBLs. Here, we employed a bioinformatics approach to identify and characterize novel B3-type MBLs from Novosphingobium pentaromativorans and Simiduia agarivorans. These enzymes may not yet pose a direct risk to human health, but their structures and function may provide important insight into the design and synthesis of a still elusive universal MBL inhibitor.展开更多
基金N.M.thanks the Science Foundation Ireland(SFI)for financial support in form of a President of Ireland Young Researcher Award(PIYRA)G.S.acknowledges the award of a Future Fellowship from the Australian Research Council(FT120100694)is grateful to the National Health and Medical Research Council of Australia for funding.
文摘Antibiotic resistance has emerged as a major global threat to human health. Among the strategies employed by pathogens to acquire resistance the use of metallo-β-lactamases (MBLs), a family of dinuclear metalloenzymes, is among the most potent. MBLs are subdivided into three groups (i.e. B1, B2 and B3) with most of the virulence factors belonging to the B1 group. The recent discovery of AIM-1, a B3-type MBL, however, has illustrated the potential health threat of this group of MBLs. Here, we employed a bioinformatics approach to identify and characterize novel B3-type MBLs from Novosphingobium pentaromativorans and Simiduia agarivorans. These enzymes may not yet pose a direct risk to human health, but their structures and function may provide important insight into the design and synthesis of a still elusive universal MBL inhibitor.
