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Polymetamorphism of the ultrahigh-temperature granulites in the Rauer Group,East Antarctica:new evidence from zircon SHRIMP U-Pb ages
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作者 TONG Laixi LIU Zhao +3 位作者 LI Chao LU Junsheng YANG Wenqiang WANG Yanbin 《Advances in Polar Science》 CSCD 2024年第2期192-205,共14页
The Rauer Group is located on the eastern margin of the early Paleozoic Prydz Belt in East Antarctica,and the typical ultrahigh-temperature(UHT,>900℃)granulites outcrop on Mather Peninsula.However,the timing of UH... The Rauer Group is located on the eastern margin of the early Paleozoic Prydz Belt in East Antarctica,and the typical ultrahigh-temperature(UHT,>900℃)granulites outcrop on Mather Peninsula.However,the timing of UHT metamorphism and P–T path of the UHT granulites have long been debated,which is critical to understanding the tectonic nature and evolution history of the Prydz Belt.Thus,both a sapphirine-bearing UHT metapelitic granulite and a garnet-bearing UHT mafic granulite are selected for zircon SHRIMP U-Pb age dating.The results show that metamorphic zircon mantles yield weighted mean^(206)Pb/^(238)U ages of 918±29 Ma and 901±29 Ma for the metapelitic and mafic granulites,respectively,while zircon rims and newly grown zircons yield weighted mean^(206)Pb/^(238)U ages of 523±9 Ma and 532±11 Ma,respectively.These new zircon age data suggest that the UHT granulites may have experienced polymetamorphism,in which pre-peak prograde stage occurred in the early Neoproterozoic Grenvillian orogenesis(1000–900 Ma),whereas the UHT metamorphism occurred in the late Neoproterozoic to early Paleozoic Pan-African orogenesis(580–460 Ma).This implies that P–T path of the UHT granulites should consist of two separate high-grade metamorphic events including the Grenvillian and Pan-African events,which are supposed to be related to assembly of Rodinia and Gondwana supercontinents respectively,and hence the overprinting UHT metamorphic event may actually reflect an important intracontinental reworking. 展开更多
关键词 ultrahigh-temperature granulites zircon SHRIMP U-Pb ages POLYMETAMORPHISM Rauer Group East Antarctica
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Bowel preparation protocol for hospitalized patients ages 50 years or older:A randomized controlled trial 被引量:1
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作者 Yu He Qi Liu +3 位作者 Yi-Wen Chen Li-Jian Cui Kai Cao Zi-Hao Guo 《World Journal of Gastrointestinal Endoscopy》 2024年第1期18-28,共11页
BACKGROUND The incidence and mortality rate of colorectal cancer progressively increase with age and become particularly prominent after the age of 50 years.Therefore,the population that is≥50 years in age requires l... BACKGROUND The incidence and mortality rate of colorectal cancer progressively increase with age and become particularly prominent after the age of 50 years.Therefore,the population that is≥50 years in age requires long-term and regular colonoscopies.Uncomfortable bowel preparation is the main reason preventing patients from undergoing regular colonoscopies.The standard bowel preparation regimen of 4-L polyethylene glycol(PEG)is effective but poorly tolerated.AIM To investigate an effective and comfortable bowel preparation regimen for hospitalized patients≥50 years in age.METHODS Patients were randomly assigned to group 1(2-L PEG+30-mL lactulose+a lowresidue diet)or group 2(4-L PEG).Adequate bowel preparation was defined as a Boston bowel preparation scale(BBPS)score of≥6,with a score of≥2 for each segment.Non-inferiority was prespecified with a margin of 10%.Additionally,the degree of comfort was assessed based on the comfort questionnaire.RESULTS The proportion of patients with a BBPS score of≥6 in group 1 was not significantly different from that in group 2,as demonstrated by intention-to-treat(91.2%vs 91.0%,P=0.953)and per-protocol(91.8%vs 91.0%,P=0.802)analyses.Furthermore,in patients≥75 years in age,the proportion of BBPS scores of≥6 in group 1 was not significantly different from that in group 2(90.9%vs 97.0%,P=0.716).Group 1 had higher comfort scores(8.85±1.162 vs 7.59±1.735,P<0.001),longer sleep duration(6.86±1.204 h vs 5.80±1.730 h,P<0.001),and fewer awakenings(1.42±1.183 vs 2.04±1.835,P=0.026)than group 2.CONCLUSION For hospitalized patients≥50 years in age,the bowel preparation regimen comprising 2-L PEG+30-mL lactulose+a low-residue diet produced a cleanse that was as effective as the 4-L PEG regimen and even provided better comfort. 展开更多
关键词 Aged 50 years or older HOSPITALIZED 2-L polyethylene-glycol+30-mL lactulose+a low-residue diet Comfort
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Survey on hospice care attitude of family members of advanced cancer patients at different ages
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作者 Xiao-Li Liu Lun-Lan Li 《World Journal of Clinical Cases》 SCIE 2024年第21期4626-4631,共6页
BACKGROUND Hospice care plays an important role in improving the quality of life of advanced cancer patients,but controversy remains over whether age affects the attitudes of family members toward hospice care.AIM To ... BACKGROUND Hospice care plays an important role in improving the quality of life of advanced cancer patients,but controversy remains over whether age affects the attitudes of family members toward hospice care.AIM To investigate the attitudes of family members of advanced cancer patients of different ages toward hospice care.METHODS The study participants were 175 family members of patients with advanced cancer from January 2020 and October 2022.The participants were divided into youth(<40 years,n=65),middle-aged(40–60 years,n=59),and elderly(>60 years,n=51)groups.Researchers investigated and compared the degree of awareness regarding hospice care,attitudes,and whether the family members of patients would choose hospice care.RESULTS Among the family members of 175 patients,approximately 28%(49/175)were aware of hospice care.Awareness of hospice care,the proportion of hospice care acceptance and adaptation attitudes,and the proportion of those who chose hospice care in the youth group were higher in the middle-aged and elderly groups(P<0.05).No statistically significant difference was found in these three indicators between the middle-aged and elderly groups(P>0.05).Hospice care was chosen mainly to relieve pain and reduce unnecessary treatment,whereas the reasons for not choosing hospice care were mainly distrust and ethical concerns.CONCLUSION The family members of patients with advanced cancer had relatively low awareness of hospice care,while youth had a higher awareness of hospice care,acceptance,and adaptation attitudes,and were more willing to choose hospice care. 展开更多
关键词 Hospice care ATTITUDE Advanced cancer Family members Age AWARENESS ACCEPTANCE
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桂枝附子汤调控AGEs/RAGE/NF-κB信号通路对类风湿关节炎大鼠的影响 被引量:9
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作者 尹谢添 赵诗超 +3 位作者 向楠 朱晓密 徐俊 张誉丹 《中成药》 CAS CSCD 北大核心 2023年第6期1809-1815,共7页
目的研究桂枝附子汤对类风湿关节炎(RA)大鼠的干预作用及对晚期糖基化终末产物(AGEs)/晚期糖基化终末产物受体(RAGE)/核转录因子-κB(NF-κB)信号通路的影响。方法将50只大鼠随机分为空白组、模型组、桂枝附子汤组(6.1 g/kg)、RAGE抑制... 目的研究桂枝附子汤对类风湿关节炎(RA)大鼠的干预作用及对晚期糖基化终末产物(AGEs)/晚期糖基化终末产物受体(RAGE)/核转录因子-κB(NF-κB)信号通路的影响。方法将50只大鼠随机分为空白组、模型组、桂枝附子汤组(6.1 g/kg)、RAGE抑制剂组(0.5 mg/kg)、桂枝附子汤+RAGE抑制剂组(6.1 g/kg+0.5 mg/kg),每组10只。用牛Ⅱ型胶原与不完全弗氏佐剂(IFA)混合乳化诱导建立RA大鼠模型,造模后连续灌胃给药30 d。给药结束后,测定大鼠关节炎指数和关节肿胀度,HE染色观察大鼠踝关节软骨及滑膜组织病理变化,ELISA法检测大鼠血清白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)水平,Western blot法检测大鼠踝关节软骨组织RAGE、p-NF-κB p65蛋白表达,免疫组化(IHC)法观察大鼠踝关节软骨组织AGE和软骨及滑膜组织TNF-α、白细胞介素-1β(IL-1β)阳性细胞表达。结果与模型组比较,桂枝附子汤组、抑制剂组和桂枝附子汤+抑制剂组均能有效降低RA大鼠关节炎指数、关节肿胀度、血清炎性因子IL-6、TNF-α水平、软骨组织AGE、RAGE、p-NF-κB p65蛋白表达和软骨及滑膜组织TNF-α、IL-1β蛋白表达(P<0.01),并能减轻软骨破坏,改善滑膜的炎性细胞浸润。结论桂枝附子汤对RA大鼠软骨和滑膜具有保护作用,该作用与抑制AGEs/RAGE/NF-κB信号通路活化,进而下调下游炎性因子表达有关。 展开更多
关键词 桂枝附子汤 类风湿关节炎 ages/RAGE/NF-κB信号通路 炎性因子
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梓醇抑制Galectin-3和CD146互作改善AGEs致肝窦内皮细胞的损伤作用 被引量:2
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作者 孙伟翔 宗莹莹 +5 位作者 刘博 庄远 秦枫 陈毓 朱善元 许惠琴 《中国畜牧兽医》 CAS CSCD 北大核心 2023年第7期2820-2831,共12页
【目的】探讨梓醇通过影响半乳糖凝集素-3(Galectin-3)和CD146的相互作用改善晚期糖基化终末产物(advanced glycation end products,AGEs)导致大鼠肝窦内皮细胞(rat liver sinusoidal endothelial cells,RLSECs)的炎性损伤作用。【方法... 【目的】探讨梓醇通过影响半乳糖凝集素-3(Galectin-3)和CD146的相互作用改善晚期糖基化终末产物(advanced glycation end products,AGEs)导致大鼠肝窦内皮细胞(rat liver sinusoidal endothelial cells,RLSECs)的炎性损伤作用。【方法】用不同浓度(0、0.1、1、10μmol/L)梓醇分别孵育RLSECs 48 h后,采用CCK-8法观察细胞增殖情况。用10μmol/L梓醇孵育RLSECs 0、12、24、48、96 h,同上法观察细胞增殖情况。设Control(空白对照组)、AGEs(AGEs处理)、Cat1(1μmol/L梓醇)、Cat10(10μmol/L梓醇)和阳性对照GB1107组(1μmol/L GB1107),Cat1、Cat10和GB1107组加入相应含量的药物培养基孵育30 min后,除Control组外其他组均在培养基中加入终浓度为200μg/mL的AGEs刺激,观察以上各组的RLSECs形态变化,采用乳酸脱氢酶(lactate hydrogenase,LDH)法检测细胞的损伤程度,采用ELISA法观察各组单核细胞趋化蛋白-1(MCP-1)、细胞间黏附分子-1(ICAM-1)的分泌量。将巨噬细胞RAW264.7种板,同上法分组并给药,48 h后采用Griess法观察各组一氧化氮(nitric oxide,NO)向细胞上清液的释放量。将RAW264.7细胞种于Transwell小室,RLSECs种于孔板底部,设Control、AGEs、Cat10、Cat10+LV-Galectin-3-GFP、Cat10+LV-Galectin-3-shRNA组,后两组先转染慢病毒48 h,再分别给药30 min后,除Control组外在培养基中加入终浓度为200μg/mL的AGEs刺激,48 h后用结晶紫法观察巨噬细胞的透膜细胞数量。将Control、AGEs、Cat10、GB1107组的RLSECs孵育药物48 h后,采用免疫荧光法观察Galectin-3和CD146的共定位情况。将Control、AGEs和Cat10组的蛋白样品通过Western blotting和免疫共沉淀(Co-IP)法检测Galectin-3和CD146的相互作用以及各自的表达量。【结果】与Control组相比,Cat0.1组RLSECs增殖率无显著差异(P>0.05),Cat1和Cat10组RLSECs增殖率显著升高(P<0.05);与孵育0 h组相比,10μmol/L梓醇孵育48 h组RLSECs增殖率显著上升(P<0.05)。因此,后续试验选用10μmol/L梓醇孵育48 h为最适试验条件。与AGEs组相比,Cat1和Cat10组改善AGEs导致的细胞损伤,RLSECs上清液LDH活力及MCP-1、ICAM-1的释放量显著降低(P<0.05)。与AGEs组相比,梓醇组抑制巨噬细胞RAW264.7的激活作用,细胞NO的释放量显著降低(P<0.05)。通过慢病毒载体过表达和敲低RLSECs和RAW264.7的Galectin-3,证明了梓醇通过抑制该分子表达可显著改善巨噬细胞的浸润作用(P<0.05)。与AGEs组相比,Cat10组Galectin-3与CD146的结合被显著抑制(P<0.05),且不影响两种分子的各自表达量(P>0.05)。【结论】梓醇通过促进AGEs致Galectin-3和CD146分子复合物的解偶联作用,改善AGEs致肝窦血管内皮细胞的损伤以及促炎因子的释放,降低巨噬细胞的激活和NO的分泌,发挥肝窦血管内皮的保护作用,通过离体试验初步证明了其发挥改善糖尿病AGEs沉积造成的肝损伤的作用机制。 展开更多
关键词 梓醇 糖尿病肝损伤 晚期糖基化终末产物(ages) GALECTIN-3 CD146 炎性损伤
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锌离子络合法快速分离纯化荞麦壳黄酮及其抗AGEs活性 被引量:1
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作者 王玥 刘子琦 +5 位作者 聂萌滋 姜鑫 李鹏程 闫梦华 朴春红 肖盛元 《中国食品学报》 EI CAS CSCD 北大核心 2023年第10期167-177,共11页
研究锌离子络合法快速分离纯化荞麦壳黄酮工艺,并评价纯化组分对晚期糖基化终末产物(AGEs)生成的抑制活性。根据络合转化率和络合强度,将荞麦壳黄酮提取物(BHF)经过二次锌离子络合,分别获得荞麦壳黄酮中间提取物(IBHF)和荞麦壳高黄酮组... 研究锌离子络合法快速分离纯化荞麦壳黄酮工艺,并评价纯化组分对晚期糖基化终末产物(AGEs)生成的抑制活性。根据络合转化率和络合强度,将荞麦壳黄酮提取物(BHF)经过二次锌离子络合,分别获得荞麦壳黄酮中间提取物(IBHF)和荞麦壳高黄酮组分(HBHF)。采用Box-Behnken响应曲面分析法优化获得IBHF组分的最佳工艺参数:乙酸锌与样品质量比2∶1,乙醇体积分数30%,氨水体积分数0.05%,室温反应10 min。在该条件下络合转化率为97.44%。IBHF组分经过二次锌离子络合,分离获得HBHF组分,其总黄酮含量为57.08 g RE/100 g(三氯化铝-乙酸盐比色法),其纯度比分离纯化前提高了6.64倍。高效液相色谱分析(HPLC)表明,HBHF组分含有异荭草素、牡荆素、异牡荆素、芦丁、山奈酚-3-O芸香糖苷5种主要黄酮,其中芦丁占比62.34%。HBHF组分表现出良好的抗AGEs活性,在质量浓度为200μg/mL时,Glu-BSA体系的中的AGEs抑制率为63.16%,MGO-BSA体系中的AGEs抑制率为42.98%,抑制率均显著优于阳性对照氨基胍。 展开更多
关键词 锌离子络合法 二次锌离子络合 荞麦壳黄酮 晚期糖基化终末产物(ages)
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基于AGEs/RAGE/NF-κB通路探讨补肝散改善D-半乳糖致衰大鼠学习记忆障碍机制研究 被引量:1
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作者 李伟 王硕 +2 位作者 王杰鹏 方芳 方朝义 《Digital Chinese Medicine》 CAS CSCD 2023年第3期317-327,共11页
目的探讨复方补肝散(BGSD)干预D-半乳糖致衰大鼠学习记忆能力的潜在作用机制。方法40只大鼠被随机分为对照组、模型组、BGSD[14.06 g/(kg·d)]组和吡拉西坦[0.4 g/(kg·d)]组,每组10只。腹腔注射D-半乳糖[400 mg/(kg·d)]... 目的探讨复方补肝散(BGSD)干预D-半乳糖致衰大鼠学习记忆能力的潜在作用机制。方法40只大鼠被随机分为对照组、模型组、BGSD[14.06 g/(kg·d)]组和吡拉西坦[0.4 g/(kg·d)]组,每组10只。腹腔注射D-半乳糖[400 mg/(kg·d)]建立衰老大鼠模型。每周记录大鼠体质量、摄水量、摄食量和抓力;八臂迷宫和跳台实验评估大鼠学习记忆能力;称取肝脏、胸腺、脾脏和脑组织重量以计算相应脏器指数;检测血清丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性;苏木精-伊红(HE)染色观察海马病理学改变;酶联免疫吸附法(ELISA)检测海马肿瘤坏死因子-α(TNF-α)、白细胞介素(IL)-6及IL-1β水平;实时荧光定量PCR(RT-qPCR)检测海马晚期糖基化终末产物受体(RAGE)、核因子-κB(NF-κB)、TNF-α、IL-6和IL-1βmRNA表达;蛋白免疫印迹法(WB)检测海马晚期糖基化终末产物(AGEs)、RAGE和NF-κB蛋白表达。结果补肝散可显著增加D-半乳糖致衰大鼠的摄食量、摄水量、体质量、抓力及脏器指数(P<0.05),减少八臂迷宫中工作记忆错误(WME)、参考记忆错误(RME)以及总记忆错误次数(TE)(P<0.05),降低跳台实验中错误次数并延长跳台潜伏期(P<0.05)。此外,补肝散可减少海马神经元损伤,提高血清SOD活性,降低MDA含量及下调促炎因子TNF-α、IL-6和IL-1β水平(P<0.05)。进一步研究结果显示,补肝散可显著降低海马AGEs、RAGE和NF-κB表达(P<0.05)。结论补肝散可通过抑制AGEs/RAGE/NF-κB信号通路调节D-半乳糖致衰大鼠神经炎症损伤从而改善学习记忆能力。 展开更多
关键词 补肝散 肝气虚 衰老 学习记忆 神经炎症 ages/RAGE/NF-κB信号通路
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AGEs交联结构:研究防治血管硬化药物的新靶标 被引量:9
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作者 钟武 王莉莉 +1 位作者 崔浩 李松 《药学学报》 CAS CSCD 北大核心 2005年第1期91-96,共6页
关键词 ages交联结构 血管硬化 ages抑制剂 ages裂解剂
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Age estimation from facial images based on Gabor feature fusion and the CIASO-SA algorithm 被引量:1
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作者 Di Lu Dapeng Wang +1 位作者 Kaiyu Zhang Xiangyuan Zeng 《CAAI Transactions on Intelligence Technology》 SCIE EI 2023年第2期518-531,共14页
Aiming at the problem of long time-consuming and low accuracy of existing age estimation approaches,a new age estimation method using Gabor feature fusion,and an improved atomic search algorithm for feature selection ... Aiming at the problem of long time-consuming and low accuracy of existing age estimation approaches,a new age estimation method using Gabor feature fusion,and an improved atomic search algorithm for feature selection is proposed.Firstly,texture features of five scales and eight directions in the face region are extracted by Gabor wavelet transform.The statistical histogram is introduced to encode and fuse the directional index with the largest feature value on Gabor scales.Secondly,a new hybrid feature selection algorithm chaotic improved atom search optimisation with simulated annealing(CIASO-SA)is presented,which is based on an improved atomic search algorithm and the simulated annealing algorithm.Besides,the CIASO-SA algorithm introduces a chaos mechanism during atomic initialisation,significantly improving the convergence speed and accuracy of the algorithm.Finally,a support vector machine(SVM)is used to get classification results of the age group.To verify the performance of the proposed algorithm,face images with three resolutions in the Adience dataset are tested.Using the Gabor real part fusion feature at 48�48 resolution,the average accuracy and 1-off accuracy of age classification exhibit a maximum of 60.4%and 85.9%,respectively.Obtained results prove the superiority of the proposed algorithm over the state-of-the-art methods,which is of great referential value for application to the mobile terminals. 展开更多
关键词 age estimation atom search algorithm feature selection Gabor feature simulated annealing
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Understory plant diversity and phenolic allelochemicals across a range of Eucalyptus grandis plantation ages
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作者 Jinjin Li Yumei Huang +3 位作者 Lianghua Chen Shun Gao Jian Zhang Danju Zhang 《Journal of Forestry Research》 SCIE CAS CSCD 2023年第5期1577-1590,共14页
Allelopathy is an important mechanism in Eucalyptus plantations that causes detrimental impacts on understory diversity.Phenolic compounds are the main allelochemicals suppressing understory plants.However,the dynamic... Allelopathy is an important mechanism in Eucalyptus plantations that causes detrimental impacts on understory diversity.Phenolic compounds are the main allelochemicals suppressing understory plants.However,the dynamic changes in phenolic allelochemicals and their relationship with understory diversity with increasing age of Eucalyptus plantations remain largely unclear.In this study,the understory plant diversity was assessed and phenolic compounds identified from leaf litter,roots,and rhizosphere soil samples in a Eucalyptus grandis plantation at two-year intervals for ten years using ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).The abundance and diversity of under story plant species were lowest in 4-year-old plantations and increased significantly with age.Seven phenolic acids and 10 flavonoids were identified from leaf litter,roots,and rhizosphere soils.Most of the potential phenolic allelochemicals,such as salicylic acid,gallic acid,4-hydroxybenzoic acid,and epicatechin,were more abundant in younger plantations,especially at4 years old.The concentrations of phenolic compounds in the rhizosphere zone were significantly lower than in litter and root samples and did not change significantly with an increase in age.Notably,phenolic compounds contributed more to the variation in the understory plants than soil factors.Hydroxyphenyllactic acid,ellagic acid,quercetin,salicylic acid,and 4-hydroxybenzoic acid were the main phenolic compounds explaining the variation in plant diversity with plantation age.These findings indicate that young E.grandis plantations,especially at four years of age,merit a greater focus because of their lower understory plant diversity and higher allelopathic potential. 展开更多
关键词 Eucalyptus grandis Phenolic compounds Understory plant diversity Plantation age
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Response of plant,litter,and soil C:N:P stoichiometry to growth stages in Quercus secondary forests on the Loess Plateau,China
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作者 Juanjuan Zhang Xinyang Li +4 位作者 Meng Chen Linjia Huang Ming Li Xu Zhang Yang Cao 《Journal of Forestry Research》 SCIE CAS CSCD 2023年第3期595-607,共13页
Ecological stoichiometry is an important indicator of biogeochemical cycles and nutrient limitations in terrestrial ecosystems.However,little is known about the response of ecological stoichiometry to plant growth.In ... Ecological stoichiometry is an important indicator of biogeochemical cycles and nutrient limitations in terrestrial ecosystems.However,little is known about the response of ecological stoichiometry to plant growth.In this study,carbon(C),nitrogen(N),and phosphorus(P)concentrations were evaluated in plant tissues(trees,shrubs,and herbs),litter,and soil of young(≤40-year-old),middle-aged(41–60-year-old),near-mature(61–80-year-old),and mature(81–120-year-old)Quercus secondary forests on the Loess Plateau,China.Vegetation composition,plant biomass,and C stock were determined to illustrate their interaction with stoichiometry.Only tree biomass C signifi cantly increased with stand development.Leaf N and trunk P concentrationsgenerally increased,but branch P decreased with growth stage.Fine roots had the highest C and P concentrations at the middle-aged stage.In contrast,shrubs,herbs,litter,and soil C:N:P stoichiometry did not change signifi cantly during stand development.Leaf N and P were positively correlated with soil C,N,P,and their ratios.However,there was no signifi cant correlation between litter and leaves in terms of C:N:P stoichiometry.A redundancy analysis showed that soil N best explained leaf N and P variance,and tree biomass and C stock were related to biotic factors such as tree age and shrub biomass.Hierarchical partitioning analysis indicated that,compared with soil or litter variables,stand age only accounted for a relatively small proportion of leaf C,N,and P variation.Thus,secondary Quercus ecosystems might have inherent ability to maintain sensitive responses of metabolically active organs to environmental factors during stand aging.The results of this work help to elucidate the biogeochemical cycling of secondary forest ecosystems in tree development,provide novel insights into the adaptation strategies of plants in diff erent organs and growth stages,and could be used to guide fertilization programs and optimize forest structure. 展开更多
关键词 Ecological stoichiometry Tree biomass C stock Understory plant Age Stand development
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可乳化共聚单体AGES用于无皂乳液聚合的研究 被引量:20
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作者 唐广粮 郝广杰 +1 位作者 宋谋道 张邦华 《高等学校化学学报》 SCIE EI CAS CSCD 北大核心 1999年第11期1804-1807,共4页
合成了一种新型可共聚单体烯烃基甘油醚磺酸盐(AGES), 不仅含有具有反应活性的烯键, 而且带有起乳化作用的磺酸离子基团及羟基, 可用于MMA/BA无皂乳液聚合体系. 对乳胶粒大小、乳液的流体力学行为及共聚物的动态力学性... 合成了一种新型可共聚单体烯烃基甘油醚磺酸盐(AGES), 不仅含有具有反应活性的烯键, 而且带有起乳化作用的磺酸离子基团及羟基, 可用于MMA/BA无皂乳液聚合体系. 对乳胶粒大小、乳液的流体力学行为及共聚物的动态力学性能进行了研究. 实验结果表明: 通过AGES的引入, 可获得直径为0.5 μm 左右的分布均匀的乳胶粒, 乳液含固量高(60% ), 粘度低, 是稳定的塑性非牛顿流体乳液, 共聚物为典型的无规共聚. 展开更多
关键词 无皂乳液聚合 ages 共聚物 MMA BA
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Stem sap flow of Haloxylon ammodendron at different ages and its response to physical factors in the Minqin oasis-desert transition zone, China
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作者 QIANG Yuquan ZHANG Jinchun +2 位作者 XU Xianying LIU Hujun DUAN Xiaofeng 《Journal of Arid Land》 SCIE CSCD 2023年第7期842-857,共16页
Haloxylon ammodendron, with its tolerance of drought, high temperature, and salt alkali conditions, is one of the main sand-fixing plant species in the oasis-desert transition zone in China. This study used the TDP30(... Haloxylon ammodendron, with its tolerance of drought, high temperature, and salt alkali conditions, is one of the main sand-fixing plant species in the oasis-desert transition zone in China. This study used the TDP30(where TDP is the thermal dissipation probe) to measure hourly and daily variations in the stem sap flow velocity of H. ammodendron at three age-classes(10, 15, and 20 years old,which were denoted as H10, H15, and H20, respectively) in the Minqin oasis-desert transition zone,China, from May through October 2020. By simultaneously monitoring temperature, relative humidity,photosynthetically active radiation, wind speed, net radiation, rainfall, and soil moisture in this region, we comprehensively investigated the stem sap flow velocity of different-aged H. ammodendron plants(H10,H15, and H20) and revealed its response to physical factors. The results showed that, on sunny days, the hourly variation curves of the stem sap flow velocity of H. ammodendron plants at the three age-classes were mainly unimodal. In addition, the stem sap flow velocity of H. ammodendron plants decreased significantly from September to October, which also delayed its peak time of hourly variation. On rainy days, the stem sap flow velocity of H. ammodendron plants was multimodal and significantly lower than that on sunny days.Average daily water consumption of H. ammodendron plants at H10, H15, and H20 was 1.98, 2.82, and 1.91kg/d, respectively. Temperature was the key factor affecting the stem sap flow velocity of H. ammodendron at all age-classes. Net radiation was the critical factor influencing the stem sap flow velocity of H.ammodendron at H10 and H15;however, for that at H20, it was vapor pressure deficit. The stem sap flow velocity of H. ammodendron was highly significantly correlated with soil moisture at the soil depths of 50and 100 cm, and the correlation was strengthened with increasing stand age. Altogether, our results revealed the dynamic changes of the stem sap flow velocity in different-aged H. ammodendron forest stands and its response mechanism to local physical factors, which provided a theoretical basis for the construction of new protective forests as well as the restoration and protection of existing ones in this region and other similar arid regions in the world. 展开更多
关键词 Haloxylon ammodendron stem sap flow stand age soil moisture water consumption Minqin oasis-desert transition zone
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老年大鼠体内NO、NOS、AGEs和SOD水平与衰老的关系 被引量:23
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作者 孔德娟 陈永春 +1 位作者 李恩 佟晓旭 《中国老年学杂志》 CAS CSCD 北大核心 1999年第3期175-176,共2页
目的探讨一氧化氮(NO)、一氧化氮合酶(NOS)、晚期糖化终末产物(AGEs)和超氧化物歧化酶(SOD)在衰老中的作用。方法测定20月龄和3月龄大鼠体内NO、NOS、AGEs和SOD的活性。结果20月龄大鼠骨胶原中A... 目的探讨一氧化氮(NO)、一氧化氮合酶(NOS)、晚期糖化终末产物(AGEs)和超氧化物歧化酶(SOD)在衰老中的作用。方法测定20月龄和3月龄大鼠体内NO、NOS、AGEs和SOD的活性。结果20月龄大鼠骨胶原中AGEs显著增加(P<0.05),血浆中NO明显高于3月龄对照组,NOS活性增高(分别为P<0.02和P<0.001),而SOD活性显著降低(P<0.02)。结论从NO生理功能可知,此结果与老年体内发生的生理变化相矛盾。推测可能由于随着年龄的增长SOD合成减少,对O÷2灭活减少,更多的O÷2与NO反应生成OONO·,使NO生物学活性降低。另一方面,AGEs通过化学反应直接灭活NO,故而导致老年体内表现出众多NO缺乏的体征。 展开更多
关键词 衰老 一氧化氮 超氧化物歧化麦 ages
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AGEs对糖尿病大鼠骨质疏松的作用机制 被引量:8
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作者 薛昊罡 冷冰 +4 位作者 马恩元 蒋维海 崔双 单广宇 苗婷婷 《中国老年学杂志》 CAS CSCD 北大核心 2011年第10期1808-1811,共4页
目的探讨AGEs对骨质疏松症的作用机制。方法取SD大鼠腹腔内注射STZ诱导建立糖尿病骨质疏松动物模型,观察AGEs血清学、骨密度、骨组织中BMP-2基因、骨密度及骨代谢的变化。结果糖尿病大鼠BMD、生物力学指标、骨组织形态计量学反映成骨活... 目的探讨AGEs对骨质疏松症的作用机制。方法取SD大鼠腹腔内注射STZ诱导建立糖尿病骨质疏松动物模型,观察AGEs血清学、骨密度、骨组织中BMP-2基因、骨密度及骨代谢的变化。结果糖尿病大鼠BMD、生物力学指标、骨组织形态计量学反映成骨活性的参数均降低,AGEs水平较正常对照组明显升高(P<0.05)。糖尿病组BMP-2 mRNA和蛋白表达水平均较正常对照组明显降低。血清AGEs与BMP-2 mRNA和蛋白表达呈显著负相关,相关系数分别为-0.715(P<0.05)、-0.621(P<0.05)。结论糖尿病大鼠血清AGEs含量显著增高,与BMP-2 mRNA和蛋白表达呈显著负相关,AGEs和BMP-2均参与了糖尿病骨质疏松的发生发展。 展开更多
关键词 ages BMP-2 糖尿病 骨质疏松
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固本健脑法对老年健忘大鼠海马组织中AGEs/RAGE信号通路的影响 被引量:8
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作者 马作峰 黄密 +2 位作者 姜瑞雪 李庆琳 孙晓利 《中华中医药学刊》 CAS 北大核心 2017年第4期954-957,I0011,共5页
目的:比较固本健脑法、补脾健脑法、补肾健脑法对老年健忘大鼠海马组织中AGEs/RAGE信号通路的影响。方法:腹腔注射D-半乳糖及Na NO2建立老年健忘大鼠模型,分别灌胃固本健脑方、归脾丸、六味地黄丸和石杉碱甲;并设空白组和模型组,灌以生... 目的:比较固本健脑法、补脾健脑法、补肾健脑法对老年健忘大鼠海马组织中AGEs/RAGE信号通路的影响。方法:腹腔注射D-半乳糖及Na NO2建立老年健忘大鼠模型,分别灌胃固本健脑方、归脾丸、六味地黄丸和石杉碱甲;并设空白组和模型组,灌以生理盐水。给药结束后,取大鼠海马组织,采用免疫组化法检测AGEs的含量,Western blot检测RAGE、NF-κB p65的表达,ELISA检测IL-1β、TNF-α的含量。结果:与空白组比较,模型组、西药组、补脾组、补肾组、固本组上述指标的表达均上调;与模型组比较,西药组、补脾组、补肾组、固本组上述指标的表达均下调;与固本组比较,西药组、补脾组、补肾组上述指标的表达下调;补脾组、补肾组、西药组组间比较,差异无统计学意义。结论:固本健脑法、补脾健脑法、补肾健脑法均能在一定程度上改善老年健忘大鼠的记忆功能,但固本健脑法作用最佳,固本健脑法改善记忆功能的分子机制与AGEs/RAGE信号通路密切相关。 展开更多
关键词 固本健脑法 老年健忘大鼠 海马组织 ages/RAGE信号通路
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甘草酸对AGEs培养肾小球系膜细胞及ECM表达的影响 被引量:6
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作者 侯绍章 郑芳芳 +1 位作者 李媛 高岭 《中国药理学通报》 CAS CSCD 北大核心 2014年第5期642-645,共4页
目的观察甘草酸(glycyrrhizic acid,GA)对糖化终产物(advanced glycation endproducts,AGEs)诱导的大鼠肾小球系膜细胞(HBZY-1)细胞增殖、细胞周期及细胞外基质成分中纤粘连蛋白(FN)和Ⅳ型胶原(C-Ⅳ)的影响。方法用含AGEs的培养液配制GA... 目的观察甘草酸(glycyrrhizic acid,GA)对糖化终产物(advanced glycation endproducts,AGEs)诱导的大鼠肾小球系膜细胞(HBZY-1)细胞增殖、细胞周期及细胞外基质成分中纤粘连蛋白(FN)和Ⅳ型胶原(C-Ⅳ)的影响。方法用含AGEs的培养液配制GA,同时设牛血清白蛋白(BSA)对照组。HBZY-1各组细胞培养48 h后,MTT检测细胞增殖,流式细胞仪分析细胞周期,ELISA方法测定细胞上清液中FN和C-Ⅳ。结果在AGEs的刺激下,细胞增殖明显,细胞S期延长,G1期缩短,PI从35.01%±4.21%增长到44.93%±0.25%(P<0.05),细胞分泌FN和C-Ⅳ明显增加(P<0.05)。加入GA后,细胞异常增殖明显减少,S期细胞减少,G1期增加,PI从44.93%±0.25%减少到42.16%±1.04%(P<0.05),细胞分泌FN和C-Ⅳ有所减少(P<0.05)。结论 GA能对抗AGEs对HBZY-1细胞的损伤作用,其可能是通过抑制AGEs诱导细胞的异常增殖和改变细胞周期来保护细胞免受AGEs的损害,另外,甘草酸能降低ECM中FN和C-Ⅳ的合成。 展开更多
关键词 甘草酸 ages 肾小球系膜细胞 细胞增殖 细胞周 ECM
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AGEs-BSA对人肾系膜细胞分泌RANTES的影响初探 被引量:8
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作者 李世云 孙子林 +2 位作者 刘乃丰 李丽 刘必成 《东南大学学报(医学版)》 CAS 2003年第6期363-365,共3页
目的 :探讨糖基化终产物 (AGEs)对人肾系膜细胞 (HRMC)分泌正常T细胞表达的调节活化蛋白 (RANTES)的影响。方法 :常规制备糖基化终产物 牛血清白蛋白 (AGEs BSA) ,干预体外培养的HRMC ,用ELISA法检测培养上清RANTES水平。结果 :AGEs BS... 目的 :探讨糖基化终产物 (AGEs)对人肾系膜细胞 (HRMC)分泌正常T细胞表达的调节活化蛋白 (RANTES)的影响。方法 :常规制备糖基化终产物 牛血清白蛋白 (AGEs BSA) ,干预体外培养的HRMC ,用ELISA法检测培养上清RANTES水平。结果 :AGEs BSA(64 0mg·L- 1 )干预HRMC后 8h ,培养液中RANTES水平开始逐步升高 ,于 48h达峰值 ,对照组BSA则未见升高 (P <0 .0 1)。结论 展开更多
关键词 ages—BSA 肾系膜细胞 RANTES T细胞 体外培养 ELISA法 糖尿病肾病
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糖尿病大鼠心肌纤维化与心肌AGEs/RAGE水平的变化 被引量:9
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作者 侯改霞 肖国强 +1 位作者 习雪峰 刘倩倩 《中国实验动物学报》 CAS CSCD 北大核心 2018年第4期461-466,共6页
目的通过构建Ⅱ型糖尿病大鼠模型,研究该模型心肌纤维化和心肌AGEs/RAGE水平的变化情况。方法雄性SD大鼠高脂饲料饲喂6周后,过夜空腹12 h,腹腔注射小剂量链脲佐菌素(STZ,30 mg/kg)。注射3、7 d后检测大鼠随机血糖浓度,两次随机血糖≥16.... 目的通过构建Ⅱ型糖尿病大鼠模型,研究该模型心肌纤维化和心肌AGEs/RAGE水平的变化情况。方法雄性SD大鼠高脂饲料饲喂6周后,过夜空腹12 h,腹腔注射小剂量链脲佐菌素(STZ,30 mg/kg)。注射3、7 d后检测大鼠随机血糖浓度,两次随机血糖≥16.7 mmol/L为Ⅱ型糖尿病大鼠建模成功。在建模成功后第4、8和12周,检测大鼠血FBG、insulin和左心室AGEs、Hyp含量及Masson染色、RAGE表达的变化。结果造模成功后第4、8和12周,糖尿病对照组(4D,8D,12D)血FBG水平均较同周次正常对照组(4C,8C,12C)显著升高,体重显著下降;造模成功后第12周时,糖尿病对照组(12D)大鼠Insulin显著高于同周次正常对照组(12C)和4周时糖尿病对照组(4D);从造模成功后第8周开始,糖尿病对照组(8D,12D)心肌Hyp、CVF、AGEs和RAGE与正常对照组(8C,12C)相比均显著升高;2型糖尿病大鼠心肌AGEs/RAGE水平与CVF呈显著正相关。结论高脂饮食饲喂加腹腔注射小剂量STZ,能够成功复制Ⅱ型糖尿病大鼠模型。随着病程的延长,心肌纤维化和心肌AGEs含量、RAGE蛋白表达均呈逐渐上升趋势,且Ⅱ型糖尿病大鼠心肌AGEs/RAGE水平与心肌纤维化存在有正相关关系。 展开更多
关键词 高脂饮食 链脲佐菌素 Ⅱ型糖尿病 心肌纤维化 ages/RAGE SD大鼠
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当归四逆汤对大鼠DPN抑制作用及AGEs/RAGE的调节 被引量:7
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作者 周晓晶 李欣 +6 位作者 柳烨惠 姜丽娟 刘红 程思宇 邢日新 明容美 张文风 《中国老年学杂志》 CAS 北大核心 2018年第22期5522-5524,共3页
目的观察当归四逆汤对糖尿病大鼠坐骨神经传导速度的影响及糖基化终末产物(AGEs)和AGEs受体(RAGE)的调节作用。方法采用链脲佐菌素(STZ)复制糖尿病大鼠模型,模型鼠随机分为中药组、西药组、模型组,另设健康鼠为正常组。分别干预8 w后,... 目的观察当归四逆汤对糖尿病大鼠坐骨神经传导速度的影响及糖基化终末产物(AGEs)和AGEs受体(RAGE)的调节作用。方法采用链脲佐菌素(STZ)复制糖尿病大鼠模型,模型鼠随机分为中药组、西药组、模型组,另设健康鼠为正常组。分别干预8 w后,检测糖尿病大鼠坐骨神经传导速度、坐骨神经病理形态、AGEs含量、RAGE mRNA表达水平。结果与模型组相比,中药能够保护坐骨神经结构,显著降低AGEs含量、下调RAGE mRNA水平(P<0. 05)。结论当归四逆汤能够通过下调AGEs/RAGE含量提高坐骨神经传导速度、保护坐骨神经结构,进而抑制大鼠糖尿病周围神经病变(DPN)的发生发展。 展开更多
关键词 当归四逆汤 周围神经病变 ages RAGE
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