Objective To compare PM2.5 pollation level between the city of coal-fuel pollution (Taiyuan) and the city of pollution mixed with coal fuels and vehicle exhausts (Beijing), to analyze the concentration of B[a]p and...Objective To compare PM2.5 pollation level between the city of coal-fuel pollution (Taiyuan) and the city of pollution mixed with coal fuels and vehicle exhausts (Beijing), to analyze the concentration of B[a]p and Pb in the pollutants, and to study the DNA damage by PM2.5. Methods Air fine particles (PM2.5) were collected in Beijing and Taiyuan by means of the filter membrane method, the concentration of B[a]p and Pb were analyzed by high performance liquid chromatography and atomic absorption spectroscopy respectveily, and the damage of DNA by PM2.5 was detected by single cell gel-electrophoresis (SCGE) using the human lung epithelial cells (A549) as target cells. Results The concentration of PM2.5 in the winter of Beijing was 0.028-0.436 mg/m3, and that in Taiyuan was 0.132-0.681 mg/m3. The concentration of B[a]p was 0.104 and 0.156 礸/mg on PM2.5 of Beijing and Taiyuan, respectively, whereas the concentration of Pb was 1.094 and 1.137 礸/mg on PM2.5 of Beijing and Taiyuan, respectively. Exposure to PM2.5 at the concentrations of 5, 50, and 200 礸/mL for 12 h and 24 h caused DNA damage of the human alveolar epithelium, and the ratios of the tailing and length of the tail were all significantly different from those of the negative control group (P<0.05), and indicated a dose-response relationship. Conclusion PM2.5 has certain genetic toxicity.展开更多
In this paper, we present the results of the evaluation of three low-cost laser sensor</span><span style="font-family:Verdana;">s</span><span style="font-family:""><...In this paper, we present the results of the evaluation of three low-cost laser sensor</span><span style="font-family:Verdana;">s</span><span style="font-family:""><span style="font-family:Verdana;"> and comparison with the standard device Metone Aerocet 531s which is capable of counting dust particles as small as 0.3 μm. The sensors used in this study are PMS5003 (Plantower), SPS30 (Sesirion), SM-UART-04L (Amphenol). During the measurement, the overall trend of the outputs from the sensors was similar to that of the Aerocet 531s. The PMS5003 sensor has a relatively small standard error in the all particle measurement ranges (<15 μg/m</span><sup><span style="font-family:Verdana;">3</span></sup><span style="font-family:Verdana;"> in the low particle concentration range). All sensors have a high linearity compared to data from standard equipment, PMS5003: PM1.0 R</span><sup><span style="font-family:Verdana;">2</span></sup><span style="font-family:Verdana;"> = 0.89;PM2.5 R</span><sup><span style="font-family:Verdana;">2</span></sup><span style="font-family:Verdana;"> = 0.95;PM10 R</span><sup><span style="font-family:Verdana;">2</span></sup><span style="font-family:Verdana;"> = 0.87;SPS30 PM2.5 R</span><sup><span style="font-family:Verdana;">2</span></sup><span style="font-family:Verdana;"> = 0.95 and PM10 R</span><sup><span style="font-family:Verdana;">2</span></sup><span style="font-family:Verdana;"> = 0.99;SM-UART-04L PM1.0 R</span><sup><span style="font-family:Verdana;">2</span></sup><span style="font-family:Verdana;"> = 0.98. Three main sensor calibration methods (single-point calibration, two-point calibration and multi-point curve correction) with implementation steps for each method as well as their practical applications in calibrating low-cost air quality sensors according to standard measuring equipment are also detailed illustrated.展开更多
基金This work was supported by National Natural Science Foundation of China (project No. 20077033).
文摘Objective To compare PM2.5 pollation level between the city of coal-fuel pollution (Taiyuan) and the city of pollution mixed with coal fuels and vehicle exhausts (Beijing), to analyze the concentration of B[a]p and Pb in the pollutants, and to study the DNA damage by PM2.5. Methods Air fine particles (PM2.5) were collected in Beijing and Taiyuan by means of the filter membrane method, the concentration of B[a]p and Pb were analyzed by high performance liquid chromatography and atomic absorption spectroscopy respectveily, and the damage of DNA by PM2.5 was detected by single cell gel-electrophoresis (SCGE) using the human lung epithelial cells (A549) as target cells. Results The concentration of PM2.5 in the winter of Beijing was 0.028-0.436 mg/m3, and that in Taiyuan was 0.132-0.681 mg/m3. The concentration of B[a]p was 0.104 and 0.156 礸/mg on PM2.5 of Beijing and Taiyuan, respectively, whereas the concentration of Pb was 1.094 and 1.137 礸/mg on PM2.5 of Beijing and Taiyuan, respectively. Exposure to PM2.5 at the concentrations of 5, 50, and 200 礸/mL for 12 h and 24 h caused DNA damage of the human alveolar epithelium, and the ratios of the tailing and length of the tail were all significantly different from those of the negative control group (P<0.05), and indicated a dose-response relationship. Conclusion PM2.5 has certain genetic toxicity.
文摘In this paper, we present the results of the evaluation of three low-cost laser sensor</span><span style="font-family:Verdana;">s</span><span style="font-family:""><span style="font-family:Verdana;"> and comparison with the standard device Metone Aerocet 531s which is capable of counting dust particles as small as 0.3 μm. The sensors used in this study are PMS5003 (Plantower), SPS30 (Sesirion), SM-UART-04L (Amphenol). During the measurement, the overall trend of the outputs from the sensors was similar to that of the Aerocet 531s. The PMS5003 sensor has a relatively small standard error in the all particle measurement ranges (<15 μg/m</span><sup><span style="font-family:Verdana;">3</span></sup><span style="font-family:Verdana;"> in the low particle concentration range). All sensors have a high linearity compared to data from standard equipment, PMS5003: PM1.0 R</span><sup><span style="font-family:Verdana;">2</span></sup><span style="font-family:Verdana;"> = 0.89;PM2.5 R</span><sup><span style="font-family:Verdana;">2</span></sup><span style="font-family:Verdana;"> = 0.95;PM10 R</span><sup><span style="font-family:Verdana;">2</span></sup><span style="font-family:Verdana;"> = 0.87;SPS30 PM2.5 R</span><sup><span style="font-family:Verdana;">2</span></sup><span style="font-family:Verdana;"> = 0.95 and PM10 R</span><sup><span style="font-family:Verdana;">2</span></sup><span style="font-family:Verdana;"> = 0.99;SM-UART-04L PM1.0 R</span><sup><span style="font-family:Verdana;">2</span></sup><span style="font-family:Verdana;"> = 0.98. Three main sensor calibration methods (single-point calibration, two-point calibration and multi-point curve correction) with implementation steps for each method as well as their practical applications in calibrating low-cost air quality sensors according to standard measuring equipment are also detailed illustrated.
