Polyphenol is an important secondary metabolite with unique physiological functions and biological activity.The polyphenols in different plants and biomass have different chemical structures,which needs various extrac...Polyphenol is an important secondary metabolite with unique physiological functions and biological activity.The polyphenols in different plants and biomass have different chemical structures,which needs various extraction methods to obtain them.Recently,plant polyphenols and their application research in food and medicine have become a research hotspot,which is mainly focused on preparation,purification,structural identification,and biological activity assays.Among these researches,extraction and separation are the key sections to investigate the structure and activity of polyphenol.Hence,this review summarized the recent extraction and separation techniques of polyphenol,including solvent extraction,supercritical fluid extraction,ultrasonic extraction,enzymatic extraction,resin adsorption extraction,and electric field method,etc.In addition,this review also reveals the current problems and proposes future extraction research of polyphenol.It is hoped that this review will provide a guide for the researchers who are actively committed to promoting progress in the field of polyphenolics.展开更多
Vapor-water two phase flow separation in pressure vessel of nuclear power plants is accomplished with swirl motion using vanes. In order to reduce separation pressure loss and to make it economic, a new type of low co...Vapor-water two phase flow separation in pressure vessel of nuclear power plants is accomplished with swirl motion using vanes. In order to reduce separation pressure loss and to make it economic, a new type of low cost simplified innovative separator using lattice core configuration is proposed where swirling is caused by the orthogonal driving flow. The performance of the separator has been assessed numerically with the commercial CFD code FLUENT 14.0. The numerical analysis is compared with the experiment. The geometry and flow conditions are chosen according to the experiment. In the analysis, standard k – e and realizable k – e turbulence models are implemented. The prediction of maximum air void fraction with realizable k – e model was almost the same as input air void fraction but the void fraction computed by standard k – e model was compared better with the experimental results than the realizable k – e model. Some discrepancies in flow pattern between the experimental and simulation results are observed which might be due to the difference of nozzle shape. However, a more detailed model is necessary to arrive at the final conclusion.展开更多
The nuclear pore complex(NPC),the sole exchange channel between the nucleus and cytoplasm,is composed of several subcomplexes,among which the central barrier determines the permeability/selectivity of the NPC to domin...The nuclear pore complex(NPC),the sole exchange channel between the nucleus and cytoplasm,is composed of several subcomplexes,among which the central barrier determines the permeability/selectivity of the NPC to dominate the nucleocytoplasmic trafficking essential for many important signaling events in yeast and mammals.How plant NPC central barrier controls selective transport is a crucial question remaining to be elucidated.In this study,we uncovered that phase separation of the central barrier is critical for the permeability and selectivity of plant NPC in the regulation of various biotic stresses.Phenotypic assays of nup62 mutants and complementary lines showed that NUP62 positively regulates plant defense against Botrytis cinerea,one of the world’s most disastrous plant pathogens.Furthermore,in vivo imaging and in vitro biochemical evidence revealed that plant NPC central barrier undergoes phase separation to regulate selective nucleocytoplasmic transport of immune regulators,as exemplified by MPK3,essential for plant resistance to B.cinerea.Moreover,genetic analysis demonstrated that NPC phase separation plays an important role in plant defense against fungal and bacterial infection as well as insect attack.These findings reveal that phase separation of the NPC central barrier serves as an important mechanism to mediate nucleocytoplasmic transport of immune regulators and activate plant defense against a broad range of biotic stresses.展开更多
基金This work was sponsored by Natural Science Foundation of Jiangsu Province(BK20180772)National Natural Science Foundation of China(31800501)Qing Lan Project.
文摘Polyphenol is an important secondary metabolite with unique physiological functions and biological activity.The polyphenols in different plants and biomass have different chemical structures,which needs various extraction methods to obtain them.Recently,plant polyphenols and their application research in food and medicine have become a research hotspot,which is mainly focused on preparation,purification,structural identification,and biological activity assays.Among these researches,extraction and separation are the key sections to investigate the structure and activity of polyphenol.Hence,this review summarized the recent extraction and separation techniques of polyphenol,including solvent extraction,supercritical fluid extraction,ultrasonic extraction,enzymatic extraction,resin adsorption extraction,and electric field method,etc.In addition,this review also reveals the current problems and proposes future extraction research of polyphenol.It is hoped that this review will provide a guide for the researchers who are actively committed to promoting progress in the field of polyphenolics.
文摘Vapor-water two phase flow separation in pressure vessel of nuclear power plants is accomplished with swirl motion using vanes. In order to reduce separation pressure loss and to make it economic, a new type of low cost simplified innovative separator using lattice core configuration is proposed where swirling is caused by the orthogonal driving flow. The performance of the separator has been assessed numerically with the commercial CFD code FLUENT 14.0. The numerical analysis is compared with the experiment. The geometry and flow conditions are chosen according to the experiment. In the analysis, standard k – e and realizable k – e turbulence models are implemented. The prediction of maximum air void fraction with realizable k – e model was almost the same as input air void fraction but the void fraction computed by standard k – e model was compared better with the experimental results than the realizable k – e model. Some discrepancies in flow pattern between the experimental and simulation results are observed which might be due to the difference of nozzle shape. However, a more detailed model is necessary to arrive at the final conclusion.
基金supported by funding from the National Natural Science Foundation of China(32250001 and 31830008 to D.X.,32150023 and 32125010 to P.L.,and 31901570 to D.W.)the National Key Research and Development Program of China(2019YFA0508403 to P.L.)+1 种基金the China Postdoctoral Science Foundation(2018M631447 to J.W.)a postdoctoral fellowship of the Tsinghua-Peking Joint Center for Life Sciences.
文摘The nuclear pore complex(NPC),the sole exchange channel between the nucleus and cytoplasm,is composed of several subcomplexes,among which the central barrier determines the permeability/selectivity of the NPC to dominate the nucleocytoplasmic trafficking essential for many important signaling events in yeast and mammals.How plant NPC central barrier controls selective transport is a crucial question remaining to be elucidated.In this study,we uncovered that phase separation of the central barrier is critical for the permeability and selectivity of plant NPC in the regulation of various biotic stresses.Phenotypic assays of nup62 mutants and complementary lines showed that NUP62 positively regulates plant defense against Botrytis cinerea,one of the world’s most disastrous plant pathogens.Furthermore,in vivo imaging and in vitro biochemical evidence revealed that plant NPC central barrier undergoes phase separation to regulate selective nucleocytoplasmic transport of immune regulators,as exemplified by MPK3,essential for plant resistance to B.cinerea.Moreover,genetic analysis demonstrated that NPC phase separation plays an important role in plant defense against fungal and bacterial infection as well as insect attack.These findings reveal that phase separation of the NPC central barrier serves as an important mechanism to mediate nucleocytoplasmic transport of immune regulators and activate plant defense against a broad range of biotic stresses.
