Control Techniques of Chinese Chestnut Yellow Crinkle

This paper initially delineates the symptoms,characteristics,and hazards associated with Chinese chestnut yellow crinkle,as well as the transmission and infection pathways of the disease.Additionally,it proposes the prevention and control strategies of the disease,including rigorous investigation and monitoring of the disease,enhanced monitoring of seedlings and scions,and integrated disease management techniques to promote the tree s health and vitality.These strategies serve to inform the prevention and control of Chinese chestnut yellow crinkle in production.

Development of Fenghuang Dancong Tea and Chestnut Sauce

To produce Fenghuang Dancong tea and chestnut sauce that is more in line with people s health needs and has better flavor,the dosage of tea,tea powder,white sugar and milk was analyzed,and the optimal formula of Fenghuang Dancong tea and chestnut sauce was studied through a single factor experiment and orthogonal experiment.The results showed that the best formula of Fenghuang Dancong tea and chestnut sauce was composed of 4.5 g of tea,1.5 g of tea powder,60 g of milk and 5 g of white sugar.The Fenghuang Dancong tea and chestnut sauce made under these conditions was light brown,and had suitable viscosity,delicate and rich taste.It had the fragrance of Fenghuang Dancong tea and the sweet aroma of chestnuts.

Effects of Different Climates and Soil Environments in the Yanshan Production Area on the Growth and Quality of Chestnuts

[Objectives] This study conducted investigations on the climate and soil nutrients of different chestnut orchards in the Yanshan region, selected key ecological factors affecting the growth and fruit quality of chestnuts, and provided a theoretical basis for the cultivation, introduction, and scientific planting of high-quality chestnuts. [Methods] The ‘Yanshanzaofeng’ chestnuts in four orchards located in Qianxi, Qianan, Funing, and Qinglong of Hebei Science and Technology Normal University were selected. The climate and soil nutrient conditions of the four orchards were investigated. Growth indicators such as branch length and diameter, nut weight, and internal quality indicators such as starch, fat, and protein content were analyzed. Principal component analysis was conducted on nine climate factors and twelve soil factors in the four chestnut orchards to identify the most closely related ecological factors influencing chestnut growth and quality. [Results] (i) Different orchards had varying climate conditions, with Qianan orchard having higher rainfall than the others, Funing orchard having the highest number of sunshine hours in the growing season, and Qinglong orchard experiencing the greatest temperature difference during the growing season. (ii) Significant differences were found in soil nutrient content among the orchards, with the coefficient of variation for organic matter and mineral elements ranging from 19.1% (S) to 80.3% (available phosphorus). (iii) The main ecological factor influencing chestnut growth was the photosynthetic factor, while fruit quality was influenced by a combination of climatic factors, photosynthetic factors, and nutrient factors. Key ecological factor indicators included: annual precipitation, annual sunshine hours, growing season precipitation, growing season sunshine hours, soil organic matter, available phosphorus, available potassium, alkali nitrogen, copper, zinc, available boron, and sulfur. Soluble solids were significantly positively correlated with growing season sunshine hours, and fat was significantly positively correlated with available potassium and alkali nitrogen, and significantly positively correlated with available phosphorus. [Conclusions] Orchards with high growing season precipitation, long sunshine hours, and high organic matter and nitrogen-phosphorus-potassium content in the soil are more conducive to promoting the growth of ‘Yanshanzaofeng’ chestnuts and improving fruit quality.

Study on the Photosynthetic Characteristics of Six Varieties(Strains)in Chinese Chestnut

[Objectives]This study was conducted to investigate the differences of photosynthetic physiological characteristics of different varieties(strains),which will provide a theoretical basis for high photosynthesis efficiency breeding and application in Chinese chestnut.[Methods]Six Chinese chestnut varieties of Castanea mollissima‘Yanbao’,C.mollissima‘Yanqiu’,C.mollissima‘Yanchang’,C.mollissima‘Yanjia’,C.mollissima‘Qianxi 37’,and C.mollissima‘Hybrid 22’were used as the materials.Using the portable photosynthesis system Li-6400,we measured the photosynthetic characteristics and diurnal variation of leaf samples of six different chestnut varieties or strains.We fitted the light response curves and photosynthetic parameters using the leaf floating model.Additionally,we determined the chlorophyll content in the leaves using a UV-visible spectrophotometer.[Results]Among the six chestnut varieties or strains,‘Yanqiu’exhibited a significantly higher photosynthetic light saturation point(P LSP)compared to other five varieties,and‘Hybrid 22’ranked second,indicating that these two varieties had the strongest adaptation to high light intensity.The photosynthetic light compensation point(P LCP)of‘Yanchang’was significantly higher than other five varieties,and"Qianxi 37"ranked second,indicating that these two varieties had the strongest adaptation to low light intensity.Additionally,they exhibited higher chlorophyll content and maintained good photosynthetic characteristics even in shaded environments with weak light stress.Varieties‘Yanbao’and‘Yanjia’showed higher P LSP and lower P LCP,indicating that these two varieties have a wider range of adaptation to light intensity.They were capable of efficiently utilizing light across a broader spectrum of intensities.‘Yanqiu’had the highest maximum net photosynthetic rate(P n,max)and the lowest dark respiration rate(R d),along with the highest chlorophyll content.It indicated that‘Yanqiu’has strong photosynthetic capacity and organic matter accumulation ability.It also had the highest P LSP,enabling it to fully utilize the high light environment of the Yanshan Mountains and possessed high light efficiency characteristics.The P n,max of‘Yanqiu’was significantly higher than other varieties.‘Hybrid 22’and‘Yanbao’also exhibited significantly higher P n,max compared with‘Yanjia’and‘Qianxi 37’.‘Yanchang’had the lowest P n,max.The order of P n,max among the six chestnut varieties or strains was as follows:‘Yanqiu’>‘Hybrid 22’>‘Yanbao’>‘Yanjia’>‘Qianxi 37’>‘Yanchang’.[Conclusions]

Effects of Chinese chestnut powder on starch digestion,texture properties,and staling characteristics of bread

Chestnut is a high nutritional value food that has been widely used as a tonic in traditional Chinese medicine.As an emerging functional food ingredient,Chinese chestnuts are rich in a range of bioactive nutrients such as starch,dietary fiber,fat,protein,trace metal element and vitamins A,B,C,D and other nutrients.In our study,Chinese chestnut powder(CCP)were added into bread formulation at 2%-6%levels(based on flour weight)to produce fresh bread with enhanced anti-staling characteristics and starch digestion inhibitory ability.The texture properties,retrogradation enthalpy,water distribution,and estimated glycemic index(eGI)of wheat bread containing CCP as a functional additive were also investigated.The results showed that incorporation of CCP apparently affected bread texture,resulting in increased hardness,as well as decreased the specific volume of wheat bread.These influences were generally proportional to the amount of CCP used.It was found that adding too much CCP resulted in a dark red color,showing increased significantly higher total color difference(ΔE)and L values.Conversely,addition of CCP significantly reduced starch digestion rate and digestion extent in bread,and the reduction degree was positively related to the amount of CCP applied.The greatest reduction in eGI value from 79.40(control)to 75.02(6%CPP bread)was observed.Meanwhile,the content of resistant starch of 6%CPP bread was about 1.36 times higher than that of control bread.CCP also reduced crumb water loss and drove the water shift from the bound to the mobile state after stored for 7 days.The retrogradation enthalpy analyses further confirmed that CCP inhibited starch retrogradation and recrystallization.These results suggested that Chinese chestnut powder could be incorporated into fresh bread to provide health functions,such as lowering potential glycaemic response and improving anti-staling characteristics of bread.

Hydrolysable Chestnut Tannin Extract Chemical Complexity in Its Reactions for Non-Isocyanate Polyurethanes(NIPU)Foams

Non-isocyanate polyurethane(NIPU)foams from a commercial hydrolysable tannin extract,chestnut wood tannin extract,have been prepared to determine what chemical species and products are taking part in the reactions involved.This method is based on two main steps:the reaction with dimethyl carbonate and the formation of urethane bonds by further reaction of the carbonated tannin with a diamine-like hexamethylene diamine.The hydroxyl groups on the tannin polyphenols and on the carbohydrates intimately linked with it and part of a hydrolysable tannin are the groups involved in these reactions.The carbohydrate skeleton of the hydrolysable tannin is also able to participate through its hydroxyl groups to the same two reactions rendering the whole molecular complex able to react to form NIPUs.The analysis by Matrix-Assisted Laser Desorption Ionization(MALDI-TOF)mass spectrometry and 13C Nuclear Magnetic Resonance(13C NMR)to further investigate the reaction mechanisms involved revealed the unsuspected complexity of chestnut hydrolysable tannin,with different fragments reacting in different manners forming a hardened network of considerable complexity.As the morphology and performance of these types of foams changes slightly with the change in the amount of glutaraldehyde and hexamine hardeners,the best performing foam formulation previously determined was scanned by SEM and analysed chemically for the structures formed.

A high level of extra-pair paternity in the Chestnut Thrush(Turdus rubrocanus)

Extra-pair copulation(EPC)can potentially maximize individual reproductive fitness,and this process may involve sexual selection of male and female traits that reflect individual quality.Previous studies have implied that adult characteristics are associated with the probability of extra-pair paternity(EPP),but it differs between species.Moreover,there are relatively few examples of the adaptive rationale for females’engagement in EPCs based on an examination of these more traditionally recorded traits,in combination with female flight-mediated traits such as wing length.We investigated whether EPP existed in the wild Chestnut Thrush(Turdus rubrocanus)population during three breeding seasons(2019–2021),and whether paternity was related to morphological traits of males and females.Eight highly variable microsatellite loci were used to identify paternity,and generalized linear mixed models were used to analyze the relationship between paternity and morphological traits.We found that EPP existed in the Chestnut Thrush.53.3%(N=41/77)of the broods contained at least one extra-pair offspring(EPO),and 34.6%(N=72/208)of the nestlings were EPO.We also found that male wing length was negatively associated with the probability of EPP and the proportion of EPO.Female body length was positively related to the probability of EPP.Both female body condition and mass were negatively associated with the proportion of EPO.While other traits of male and female did not relate to the probability of EPP or the proportion of EPO.Extra-pair males had better body condition compared to the males they cuckolded.EPO did not differ from their half-siblings in terms of body size or body condition.The results suggest that body size and body condition were associated with EPP in the Chestnut Thrush.This study provides fundamental information for further studies on the evolution and maintenance of EPP in the Chestnut Thrush,and it is also useful for the comparison of EPP among Turdus species.

Effects of Different Sample Parts and Sterilization Methods on Contamination and Survival Rates of Shoots with Buds in Chinese Chestnut

In this study,we investigated the preferable explant types and sterilization method for tissue culture in Chinese chestnut,in order to provide a technical support for the asexual reproduction of Chinese chestnut.The base,middle and apex parts of annual shoots with buds in Chinese chestnut were sampled and cut to 2-3 cm stem segments each with one bud,then sterilized orderly with different duration in 2%NaClO plus 0.1%HgCl_(2)solutions.The results indicated that the duration of 2%NaClO for 20 min+0.1%HgCl_(2)for 15 min exerted an effective disinfection property on the middle parts of annual shoots with buds,and achieved a contamination rate under 5%and a survival rate over 90%.

板栗全生长季树干液流及蒸腾耗水特征

【目的】明确板栗(Castanea mollissima Bl.)蒸腾耗水特征,为板栗园制定科学合理的水分管理措施提供理论参考。【方法】采用热扩散探针法,对‘大板红’板栗植株全生长季树干液流速率进行连续监测,并同步观测7个相关环境因子:太阳辐射(solar radiation,SR)、水汽压亏缺(vapor pressure deficit,VPD)、空气温度(air temperature,AT)、空气相对湿度(air relative humidity,ARH)、风速(wind speed,WS)、土壤温度(soil temperature,ST)和土壤湿度(soil relative humidity,SRH),解析板栗树干液流规律和耗水特征及其与环境因子间的关联性。【结果】板栗树干明显液流启动于5月3日,结束于10月26日,前后历时176 d。板栗全生长季晴天树干液流速率日变化呈单峰“几”字形曲线,其在6、7、8月每日启动到达峰值时间早于5、9、10月,而下降时间晚于5、9、10月,致6、7、8月份液流速率峰值持续时间长于5、9、10月。板栗全生长季液流速率日均值、日均耗水量和月耗水量由高到低排序均为8月>7月>6月>9月>5月>10月,其中显著性检验表明7月和8月之间无显著差异,但显著高于其他月份。夜间液流量占比由高到低排序为10月>9月>5月>6月>7月>8月,其中显著性检验表明10月、9月和5月显著高于6月、7月和8月。板栗全生长季树干液流速率与太阳辐射、水汽压亏缺、空气温度和风速均呈极显著正相关,而与空气相对湿度均呈极显著负相关。分别建立了5—10月板栗树干液流速率与7个环境因子的6个回归模型。【结论】板栗树干有明显液流始于5月初、终于10月末;7—8月是板栗的关键需水期;建立的板栗植株液流速率与环境因子的回归模型,可用于通过环境因子估测不同月份及整个生长季的植株耗水量。

谷氨酰胺转氨酶对板栗粉面团理化特性的影响

为改善板栗粉在无麸质食品领域的品质特性及加工性能,考察谷氨酰胺转氨酶(glutamine transaminase,TG)添加量分别为0、0.9、1.8、2.7、3.6 U/g(按板栗全粉质量计)时对板栗粉面团质构特性、流变特性、糊化特性等的影响,并从游离巯基含量的变化加以解释,最后通过扫描电子显微镜观察板栗面团的微观结构。结果表明:TG的添加可以增强板栗面团的黏度、硬度、弹性、咀嚼性和回复性,与空白对照相比,添加量为2.7 U/g时,其最终黏度增加6.8 cP、硬度增加7.55 g、弹性增加0.38、咀嚼性增加33.62 g、回复性增加0.118,板栗面团内部的结构更加稳定;随着TG添加量的增加,板栗面团的弹性模量和黏性模量均增加,综合黏弹性上升,抗外界形变能力增强,面团稳定性及加工性能随之提高;板栗面团游离巯基的含量随TG添加量的增加而减少,添加量为2.7 U/g与未添加时的相比,游离巯基含量减少了0.16 mmol/g。但TG添加量过多会导致蛋白过度交联,淀粉粒被迫外露,面团整体的稳定性降低。综上,当TG添加量为2.7 U/g时,改善板栗面团的理化特性效果达到最佳。

荸荠收获机弹簧辊式泥果分离装置研制

针对荸荠收获泥果分离难、果实损伤率高的问题,根据旱地环境下荸荠采收作业需求,该研究提出一种由正反旋弹簧并排布置的荸荠收获机弹簧辊式泥果分离装置。通过对荸荠与弹簧辊的相对运动过程动力学分析,确定了影响荸荠收获泥果分离的关键因素为弹簧外径、螺距、相邻弹簧间距、高度差及弹簧转速、线径、作业速度。利用EDEM软件建立泥果混合物离散元模型,对弹簧辊结构参数与工作参数进行单因素试验,分析各因素对泥果分离效果的影响。以荸荠筛分率和土壤筛分率为指标进行二次回归正交试验,得到弹簧辊最佳参数组合为外径100 mm、螺距30 mm、间距9 mm、转速420 r/min,该参数组合下荸荠筛分率为80.00%,土壤筛分率为80.69%。进行仿真验证试验,对比试验结果与模型预测值,荸荠筛分率平均相对误差为2.09%,土壤筛分率平均相对误差为2.42%。以明果率、伤果率、破皮率、挖净率为指标开展模拟采挖试验,分析不同线径弹簧辊的泥果分离能力,确定12 mm线径弹簧兼具较好的振动筛分性能和较低的损伤率。通过实际收获试验测得作业速度0.21 m/s,作业效率0.19 m^(2)/s,碎土率75.61%,明果率82.42%,伤果率14.73%,破皮率7.01%。研究结果可为荸荠收获机研制和优化改进提供参考。

大别山板栗片热泵干燥特性及动力学模型分析

为了研究大别山板栗片热泵干燥过程和特性,建立其干燥动力学模型,并优化干燥工艺以提高干燥后的品质,以大别山板栗为研究对象进行热泵干燥动力学研究,通过对7种常用干燥模型线性化处理,筛选适合用来描述板栗片热泵干燥特性的数学模型,并利用试验数据与模型进行回归拟合,确定并建立最适合的数学模型。结果表明:Page模型的预测值曲线和实测值曲线高度吻合,Page模型可用来描述板栗片热泵干燥过程中水分的变化规律。研究结果可为板栗片实际生产中的热泵干燥加工提供一定参考。

外源甲基乙二醛(MG)对盐胁迫下板栗幼苗生理特性的影响

甲基乙二醛(MG)为近年来发现的新兴信号分子,在调控植物发育和抗逆过程中具有重要作用。为了探讨MG缓解盐胁迫对板栗幼苗损伤的生理机制,以板栗品种‘蒙早’2 a生幼苗为试验对象,研究了施用0.5 mmol/L MG及其清除剂N-乙酰半胱氨酸(NAC)对盐胁迫(NaCl含量200 mmol/L)下板栗幼苗抗氧化系统、乙二醛酶系统和渗透调节物质含量的影响。结果表明:施用外源MG能够提高盐胁迫下板栗幼苗抗氧化酶(SOD、POD、CAT、APX、GR)的活性,激活乙二醛酶系统(GlyI和GlyII酶活性提高),调节AsA—GSH循环,增加抗氧化剂(GSH、AsA)含量以及渗透调节物质脯氨酸(Pro)在叶片中的积累,减少丙二醛(MDA)、MG和超氧阴离子(O2-)的积累,缓解盐胁迫下板栗幼苗遭受的MG胁迫、氧化胁迫和渗透胁迫伤害,增强板栗幼苗的耐盐性;而添加MG清除剂NAC后,抑制了MG的缓解损伤效应。MG对增强板栗的耐盐性具有积极作用,研究MG作为新型外源调节物质调控植物抗逆胁迫为拓展板栗适用范围和盐碱地区板栗资源挖掘提供了重要的理论依据。

高产大果抗寒日本栗新品种‘黄丰’选育研究

该研究为了选育出丰产、大果、优质且抗寒的日本栗新品种,通过引种驯化和育种研究,成功培育出高产大果抗寒日本栗新品种‘黄丰’。该品种产量稳定,嫁接10 a以上平均产量高达6255 kg·hm^(-2),且果实硕大,平均单果质量达27.17 g,加工品质上乘。在抗寒性方面,‘黄丰’能够抵御-30℃的极端低温,并具备抗栗瘿蜂的能力。该品种在辽宁省丹东、大连等日本栗适宜栽培区具有广阔的种植前景。

响应面法优化板栗酥性饼干配方研究

为开发一款板栗饼干,研究以板栗粉、低筋面粉为主要原料,以感官评分为指标,通过单因素试验确定板栗粉、麦芽糖醇、黄油和牛奶的最佳添加量,并在此基础上利用响应面法,确定板栗酥性饼干的最佳配方。最佳配方为以低筋面粉质量为基准,板栗粉添加量33%,麦芽糖醇添加量30.7%,黄油添加量26.3%,牛奶添加量36%,聚葡萄糖2%、小苏打0.6%。所得板栗酥性饼干营养丰富,具有较高的应用价值。

基于改进YOLOv8模型的轻量化板栗果实识别方法

为实现自然环境下的板栗果实目标快速识别,该研究以湖北省种植板栗为研究对象,提出了一种基于改进YOLOv8模型的栗果识别方法 YOLOv8-PBi。首先,将部分卷积(partial convolution,PConv)引入C2f模块中,缩减卷积过程中的浮点数和计算量;其次,引入加权双向特征金字塔网络(bidirectional feature pyramid network,BiFPN),增强多尺度特征融合性能,最后,更改边界框损失函数为动态非单调聚焦机制WIoU(wise intersection over union,WIoU),提高模型收敛速度,进一步提升模型检测性能。试验结果表明,改进YOLOv8-PBi模型准确率、召回率和平均精度分别为89.4%、74.9%、84.2%;相比原始基础网络YOLOv8s,模型权重减小46.22%,准确率、召回率和平均精度分别提升1.3、1.5、1.8个百分点。部署模型至边缘嵌入式设备上,经过TensorRT加速后,检测帧率达到43帧/s。该方法可为板栗智能化收获过程中的栗果识别提供技术基础。

板栗WRKY基因家族鉴定及其在干旱胁迫下的表达分析

WRKY转录因子在植物生长和防御反应的调控中发挥着重要作用。为了探究WRKY基因家族在板栗抗旱中的功能,对板栗WRKY基因家族成员进行了鉴定,对其编码蛋白的理化性质、系统发育、结构等进行分析,并通过转录组测序和qRT-PCR技术分析其在干旱胁迫下的表达特征。通过生物信息学技术在板栗中共鉴定到65个WRKY基因家族成员,将其分为Ⅰ、Ⅱ、Ⅲ3组,其中Ⅱ组根据其结构和系统发育关系分为5个亚组。WRKY基因的结构和保守基序分析表明,外显子数目为1~7个,同一亚组的外显子数目和基序分布类似。WRKY基因保守结构域发生了一定的变异,包括WRKY七肽结构域的缺失,锌指结构的缺失,七肽结构域变异为WRKYGKK、WRKYGRK和WRKYGRK等。转录组测序数据表明,有4个WRKY基因家族成员在样品中没有表达,干旱胁迫诱导表达的WRKY基因家族成员表达量上调的有49个,而表达量下调的WRKY家族成员有11个。以上结果表明,板栗WRKY基因在对干旱胁迫的响应中发挥了重要作用。

茶多酚对混浊板栗汁抗沉稳定性及消化特性的影响

该文以板栗和茶多酚为主要原料研制茶多酚板栗汁,旨在探究茶多酚对混浊型板栗汁的稳定性和对板栗淀粉消化特性的影响。通过在板栗汁中加入没食子酸乙酯(ethyl gallate,EG)、表没食子儿茶素没食子酸酯(epigallocatechin gallate,EGCG)、表儿茶素没食子酸酯(epicatechin gallate,ECG)、表没食子儿茶素(epigallocatechin,EGC)、表儿茶素(epicatechin,EC)和混合茶多酚,发现5种单体茶多酚对稳定性影响最小的添加质量浓度均为0.8 mg/mL;单体EC对板栗汁的稳定性影响最小,混合茶多酚最容易导致板栗汁不稳定。板栗汁的流变学分析表明,茶多酚可通过分子间力使板栗汁中的淀粉颗粒聚集,导致板栗汁黏度降低,从而容易发生沉降。加入茶多酚会使板栗汁的消化受到抑制,其中加入EG和EGCG的板栗汁抑制效果较好。根据透光率、沉降体积和体外消化试验的结果可以发现茶多酚的加入会对板栗汁的稳定性和板栗淀粉的消化特性产生影响,可为茶多酚板栗汁复合饮料的研制提供参考。

外源槲皮素对干旱胁迫下板栗苗抗氧化功能的影响

【目的】探明外源槲皮素(Quercetin,Q)提高板栗苗耐旱性的适宜喷施浓度及其生理机制。【方法】以燕宝(YB)板栗苗为试材,通过盆栽控水的方法,以重度干旱胁迫CK1和正常管理CK2为对照,研究在重度干旱胁迫下喷施不同浓度[600μmol·L^(-1)(QD1)、1200μmol·L^(-1)(QD2)、1800μmol·L^(-1)(QD3)和2000μmol·L^(-1)(QD4)、3000μmol·L^(-1)(QD5)]槲皮素对板栗幼苗叶绿素含量、抗氧化酶活性、丙二醛(MDA)和黄酮醇含量的影响。【结果】与同处于重度干旱胁迫下未经槲皮素喷施处理的CK1相比,5种不同浓度槲皮素喷施处理均能显著提高板栗叶片叶绿素含量、超氧化物歧化酶(SOD)活性、过氧化氢酶(CAT)活性、过氧化物酶(POD)活性和黄酮醇含量,同时显著降低了板栗叶片丙二醛(MDA)含量,但喷施后板栗苗的状态仍不能恢复到正常管理的CK2。通过主成分分析及隶属函数法,得出QD1、QD2、QD3、QD4、QD5各处理的平均抗旱性度量值分别为0.735、0.641、0.738、1.389、0.828。其中2000μmol·L^(-1)(QD4)槲皮素浓度处理效果更显著。【结论】外源施加槲皮素可以通过提高板栗幼苗抗氧化酶活性、叶绿素含量,减缓MDA增加速度,减缓细胞膜质过氧化的速度,以减轻干旱对板栗叶片细胞的伤害,从而提高板栗幼苗耐旱性,且以2000μmol·L^(-1)(QD4)槲皮素浓度处理效果最好。

板栗PAT基因家族成员鉴定及不同胁迫响应分析

【目的】鉴定和分析板栗PAT基因家族及其对不同胁迫的响应,探究板栗PAT基因家族的抗逆功能。【方法】在板栗全基因组水平上进行搜索和鉴定板栗PAT基因家族成员,利用生物信息学方法研究其系统发育进化树、基因结构和motif、蛋白理化性质、染色体定位、共线性和启动子顺式元件等。以燕山红栗为试验材料,分析盐胁迫、抗病胁迫和干旱胁迫处理对板栗PAT基因家族表达模式的影响。【结果】在板栗基因组中共鉴定出包含DHHC结构域的21个PAT基因家族成员,他们与24个AtPAT基因家族成员共聚集为6个亚组;大多数CmPAT家族成员为具有亲水性的碱性稳定蛋白;21个PAT基因家族成员不均匀地分布在板栗的9条染色体上;在CmPAT基因启动子区域鉴定到多种非生物胁迫及激素响应元件;表达模式分析表明,多个CmPAT基因不同程度地参与抗病、干旱、盐胁迫响应。【结论】共鉴定了21个板栗PAT基因家族成员,同时筛选到CmPAT24、CmPAT7、CmPAT14可能共同参与了盐胁迫和干旱胁迫的调控,CmPAT7可能共同参与盐胁迫、干旱胁迫、抗病胁迫的调控。