BACKGROUND Few reports have described living foreign bodies in the human body.The current manuscript demonstrates that computed tomography(CT)is an effective tool for accurate preoperative evaluation of living foreign...BACKGROUND Few reports have described living foreign bodies in the human body.The current manuscript demonstrates that computed tomography(CT)is an effective tool for accurate preoperative evaluation of living foreign bodies in clinic.The threedimensional(3D)reconstruction technology could clearly display anatomical structures,lesions and adjacent organs,improving diagnostic accuracy and guiding the surgical decision-making process.CASE SUMMARY Herein we describe a 68-year-old man diagnosed with digestive tract perforation and acute peritonitis caused by a foreign body of Monopterus albus.The patient pre-sented to the emergency department with complaints of dull abdominal pain,profuse sweating and a pale complexion during work.A Monopterus albus had entered the patient’s body through the anus two hours ago.During hospitalization,the 3D reconstruction technology revealed a perforation of the middle rectum complicated with acute peritonitis and showed a clear and complete Monopterus albus bone morphology in the abdominal and pelvic cavities,with the Monopterus albus biting the mesentery.Laparoscopic examination detected a large(diameter of about 1.5 cm)perforation in the mid-rectum.It could be seen that a Monopterus albus had completely entered the abdominal cavity and had tightly bitten the mesentery of the small intestine.During the operation,the dead Monopterus albus was taken out.CONCLUSION The current manuscript demonstrates that CT is an effective tool for accurate preoperative evaluation of living foreign bodies in clinic.展开更多
[Objectives]The effect and mechanism of grape seed and sweet wormwood extract on growth of Monopterus albus were studied.[Methods]1 500 healthy M.albus with average mass of 23.8 g/tail were randomly divided into 5 gro...[Objectives]The effect and mechanism of grape seed and sweet wormwood extract on growth of Monopterus albus were studied.[Methods]1 500 healthy M.albus with average mass of 23.8 g/tail were randomly divided into 5 groups.The control group was fed with basal diet and the experimental groups were fed with 10,20,30 and 40 g/kg of grape seed and sweet wormwood extract,respectively.The growth performance of the fish was measured after 60 d of feeding at 23-28℃.[Results]The addition of grape seed and sweet wormwood extract in basal diet could increase the body weight gain rate,specific growth rate and protein efficiency,and decrease the feed coefficient.The activity of digestive enzymes,glutamic-oxaloacetic transaminase,glutamic-pyruvic transaminase,blood alkaline phosphatase and serum total protein was increased,and the contents of blood glucose and triglyceride were decreased.Compared with the control group,the above indexes showed significant difference(P<0.05)when the dosage was 20 and 30 g/kg(P<0.05).The difference was not significant(P>0.05)when the dosage was 10 and 40 g/kg(P>0.05).[Conclusions]As a feed additive,the plant extract from grape seed and sweet wormwood could increase digestive enzyme activity and regulate blood biochemical parameters of fish body.It had the function of promoting the digestion and absorption,enhancing the metabolic activity of 3 main nutrients,and hastening the growth of fish.展开更多
Seedlings of the medicinal plant Hyoscyamus albus were supplied with an excess of Cu to examine the possible application in phytoremediation. The seedlings were cultured in B5 medium supplied with basal 0.1 μM Cu and...Seedlings of the medicinal plant Hyoscyamus albus were supplied with an excess of Cu to examine the possible application in phytoremediation. The seedlings were cultured in B5 medium supplied with basal 0.1 μM Cu and 200 μM Cu under various light conditions: short day (SD);long day (LD);and continuous light (CL). In addition, the effect of supplying 200 μM Cu under Fe deficiency was determined, in order to elucidate the interaction between Cu and Fe. Interestingly, Fe-deficiency symptoms that developed in plants grown with basic levels of Cu under LD almost disappeared when excess Cu was supplied. Plant growth mainly depended on the photo irradiation period (SD < LD^CL);and 200 μM Cu did not inhibit growth at all when Fe was available, whereas in the absence of Fe, CL caused damage to growth. Analysis of the Cu and Fe contents of the plants revealed that Cu was distributed equally in both the aerial parts and roots, whereas most of the Fe was found in the roots;under Fe deficiency, Cu accumulation in the roots apparently increased. Cu was mainly distributed in the soluble fraction, which included vacuoles and the cell-wall fraction. These results provide evidence indicating that H. albus seedlings are tolerant of Cu present in excess. Furthermore, excess Cu was able to compensate for Fe deficiency, depending on the light conditions. Continuous light inhibited this effect, probably as a result of the induction of Mn deficiency. The possible applications of this newly discovered cuprophyte are discussed.展开更多
L. albus is an annual grain-legume crop mainly grown for high-protein fodder worldwide but also to produce large seeds for human consumption as a snack-food. In order to make genetic gains in grain yield, assessment o...L. albus is an annual grain-legume crop mainly grown for high-protein fodder worldwide but also to produce large seeds for human consumption as a snack-food. In order to make genetic gains in grain yield, assessment of the genetic variation in the germplasm and identification of loci associated with agronomic traits are essential. Phomopsis blight (PB) and Pleiochaeta root rot (PRR), caused by the fungal pathogens Diaporthe toxica and, Pleiochaeta setosa respectively, are two major yield-limiting diseases of the L. albus crop. The extent of genetic diversity in 94 accessions of L. albus comprising: Australian and exotic cultivars, advanced breeding lines, and landraces originating from 26 different countries was determined utilizing PCR-based genic, and microarray-based Diversity Arrays Technology (DArT™), markers. All accessions were evaluated for resistance to PB in two plant tissues (leaves and stems) using either sprayed or injected spore inoculum. A subset of 58 accessions was further evaluated for resistance to PRR by growing seedlings in spore-infested potting mix. The combined data of 724 (50 genic- and 674 DArT) markers were used for cluster analysis. A subset of 324 markers with call rate ≥95% and predicted disease scores of different genotypes were used to identify marker loci accounting for phenotypic variation in PB and PRR resistance using linear regression analysis. Several markers showed significant association with PB or PRR resistance at P < 0.05. Our results showed that favourable alleles for PB and PRR resistance are present in the diverse accessions investigated and they will provide valuable materials for lupin breeding.展开更多
The current study has been undertaken to examine the beneficial effect in the power output of a microbial fuel cell (MFC) by adding cellulolytic bacteria Ruminococcus albus (R. albus) into the anodic chamber. Mediator...The current study has been undertaken to examine the beneficial effect in the power output of a microbial fuel cell (MFC) by adding cellulolytic bacteria Ruminococcus albus (R. albus) into the anodic chamber. Mediator-less H-type MFCs were set up where the anode chamber contained anaerobic digester microorganisms as inocula on finely ground pine tree (Avicel) at 2% (w/v) and the cathode chamber of 10mM phosphate buffered saline conductive solution, both separated by a cation exchange membrane. The functioning of the MFCs for generation of electrical power and the amounts of gaseous byproducts was monitored over a 9-day period. The addition of cellulolytic bacteria caused an increase of average power density from 7.9 m W/m2 to19.5 m W/m2, about 245% increase over a 9-day period. For both groups of MFCs;with R. albus and the control, the head space gases collected were methane and CO2. While the methane: CO2 ratios were found unchanged at 1.7:1 throughout the 9 days of operation, the total gas production increased from 248 mL to 319 mL due to the presence of R. albus addition. This study confirms that whereas the biocatalytic activity of anode microbial population determines the energy production, the addition of external cellulolytic bacteria into anode microbial population can improve and extend the biomass utilization.展开更多
[Objective] This study aimed to explore the mutagenesis effects of N＋ ion beam implantation on Streptomyces a/bus and obtain high-yield salinomycin- producing mutant strain. [ Method ] Streptomyces a/bus strain S-11-...[Objective] This study aimed to explore the mutagenesis effects of N＋ ion beam implantation on Streptomyces a/bus and obtain high-yield salinomycin- producing mutant strain. [ Method ] Streptomyces a/bus strain S-11-04 was mutated with different doses of N ＋ implantation. The effects of low energy N ＊ implantation on the survival rate, colony morphology and salinomycin-producing ability were investigated. [ Result] The results showed that low energy N ＋ implantation can efficiently improve the positive mutation rate of Streptomyces albus; 13 mutant strains with high yield of salinomycin were isolated; to be specific, mutant strain N3- 6 has relatively good genetic stability with four continuous generations, and the titres of salinomycin were increased by 41% in the shake-flask culture and 20.5% in mass production compared with the control. [ Conclusion ] N ＋ ion beam irradiation is an effective method to obtain high-yield salinomycin-producing Streptomy- ces albus strain.展开更多
The swamp eel(Monopterus albus)is a commonly cultured freshwater fish.Selection and cultivation of fecund broodstock is crucial for efficient artificial reproduction in this species.However,there are currently no guid...The swamp eel(Monopterus albus)is a commonly cultured freshwater fish.Selection and cultivation of fecund broodstock is crucial for efficient artificial reproduction in this species.However,there are currently no guidelines for selecting high-quality males in M.albus.This study quantitatively investigated the sperm motility of male swamp eels during the breeding season by using computer-assisted sperm analysis system.Variability in the proportion and number of motile sperm was observed between individual males.The testes were grouped into high-quality and low-quality according to sperm quality.Morphometric parameters of the two groups were analyzed.We found that high-quality testes were longer(150±27 mm verses 127±19 mm)than low-quality testes.Besides,the proportion of testes with a dark appearance was slightly higher(58.3%verses 48.1%)in the high-quality group than in the low-quality group.Metabolomic analysis was conducted to compare the metabolite profiles of high-quality and low-quality testes.Glycerophospholipids,organic acids,glycerolipids,amino acids,sphingolipids,and nucleotides were the most abundant metabolites in the testes of swamp eels.Moreover,135 differential metabolites were identified.Several nucleotide derivatives,including 50-deoxyadenosine,20-deoxyadenosine,and ribosyl adenosine,were the most significantly enriched metabolites in highquality testes.These metabolites are potential indicators for the testis quality in M.albus.Altogether,our work provides systematic and quantitative data about the physiological and biochemical characteristics of M.albus testis,which provides an evidential basis for the selection of high-fecundity parents to improve artificial reproduction efficiency of M.albus.展开更多
Dear Editor,Streptomyces can produce a large variety of secondary metabolites as a major source of anti-infective, antitumor or immune-suppressive agents widely applied in clinical treatment. Antibiotics-resistant bac...Dear Editor,Streptomyces can produce a large variety of secondary metabolites as a major source of anti-infective, antitumor or immune-suppressive agents widely applied in clinical treatment. Antibiotics-resistant bacteria are spreading at alarming rates.展开更多
Abstract: A split-root system was used to investigate whether the external or internal P concentration controls root cluster formation and citrate exudation in white lupin (Lupinus albus L.) grown under controlled con...Abstract: A split-root system was used to investigate whether the external or internal P concentration controls root cluster formation and citrate exudation in white lupin (Lupinus albus L.) grown under controlled conditions. In spite of low P concentrations in the shoots and roots of the -P plant, its dry weight was not reduced compared with the +P plant. Supplying external P (0.25 mmol/L) to one root half resulted in an increase in P concentration not only in the shoot, but also in the P-deprived root half, indicating P cycling within the plants. Omitting P from both split-root pots stimulated root cluster formation in both root halves, whereas P supply to one root half stimulated root cluster formation at the beginning of the treatment. Neither P supply to just one root half continuously nor resupply of P to one root half after 19 d of P starvation inhibited root cluster formation on the P-deprived side, although the concentration of P in this root half and shoot increased markedly. The results indicate that root cluster formation in L. albus is controlled by both shoot and root P concentrations. The rates of citrate exudation by both root halves with P deficiency were higher than those of the one root half supplied with P only. In the treatment with one root half supplied with P, the rates of citrate exudation by either the P-supplied or -deprived root halves were almost the same, regardless of P concentration in the roots. The results suggest that internal P concentration controls root cluster formation and citrate exudation in white lupin, but these processes may be regulated by different mechanisms.展开更多
IN our previous studies, we have used in situ nick translation techniques for analysis of DNaseⅠ sensitivity and restriction enzymes in situ cleavage on pachytene bivalents of rice-field eels(Monopterus albus Zuiew)....IN our previous studies, we have used in situ nick translation techniques for analysis of DNaseⅠ sensitivity and restriction enzymes in situ cleavage on pachytene bivalents of rice-field eels(Monopterus albus Zuiew). A characteristic construction of bivalents--"the lampbrush-likechromosome", which has not been reported yet, was detected in some pachytene bivalents.展开更多
为了研究黄鳝(Monopterus albus)热休克蛋白70(Heat Shock Protein 70,简称HSP70)的结构和功能,试验采用生物信息学方法对黄鳝HSP70的基本理化性质、亲/疏水性、信号肽、磷酸化位点、糖基化、无序化特征、二级结构、三级结构、相互作用...为了研究黄鳝(Monopterus albus)热休克蛋白70(Heat Shock Protein 70,简称HSP70)的结构和功能,试验采用生物信息学方法对黄鳝HSP70的基本理化性质、亲/疏水性、信号肽、磷酸化位点、糖基化、无序化特征、二级结构、三级结构、相互作用蛋白网络及进化树进行了研究。结果表明,黄鳝HSP70由441个氨基酸组成,相对分子质量为48146.31,等电点为5.98,整体呈碱性,是较稳定的亲水性蛋白质,有信号肽,但不存在跨膜结构,属于分泌型蛋白质。黄鳝HSP70存在35个潜在磷酸化位点,其中包括19个Ser、14个Thr和2个Tyr磷酸化位点,有5个典型的N糖基化位点。二级结构以α-螺旋(39.68%)、延伸链(20.41%)、β-折叠(6.58%)和无规则卷曲(33.33%)为主要结构。热休克蛋白70(HSP70)作为分子伴侣,在蛋白质折叠和运输、细胞周期调控、细胞凋亡和精子发生等方面发挥着重要作用。研究表明,黄鳝HSP70在抵抗外界应激源、环境应激保护等方面起着重要作用。展开更多
The plant natural product scopolin,a coumarin secondary metabolite,has been extensively exploited in flavor,cosmetic,medicine,and other industrial fields.Melilotus albus,a leguminous rotation crop,contains high concen...The plant natural product scopolin,a coumarin secondary metabolite,has been extensively exploited in flavor,cosmetic,medicine,and other industrial fields.Melilotus albus,a leguminous rotation crop,contains high concentrations of coumarin.The transcriptional regulatory network that controls the flow through the scopolin biosynthesis pipeline in M.albus remains poorly understood.MabHLH11 encodes a basic helix–loop–helix(bHLH)transcription factor whose transcription is positively associated with scopolin accumulation and with the expression of MaMYB4,the bHLH partner of the MYB-bHLH complex.Phylogenetic analysis grouped MabHLH11 in the TRANSPARENT TESTA 8(TT8)clade of the bHLH IIIf subgroup.The MabHLH11 protein contained an MYB-interacting region and physically interacted with MaMYB4 in yeast and tobacco leaves.Co-overexpression of MabHLH11 with MaMYB4 in M.albus additively increased the expression of UDP-glucosyltransferase(MaUGT79)and induced more scopolin accumulation than occurred under the expression of MabHLH11 alone.MabHLH11 directly targeted the promoter of MaUGT79 and the activation of MabHLH11 was strengthened by the presence of MaMYB4.Thus,MaMYB4 enhanced the function of MabHLH11 in upregulating scopolin biosynthesis in M.albus,providing a theoretical basis for scalable production of a high-value plant natural product.展开更多
文摘BACKGROUND Few reports have described living foreign bodies in the human body.The current manuscript demonstrates that computed tomography(CT)is an effective tool for accurate preoperative evaluation of living foreign bodies in clinic.The threedimensional(3D)reconstruction technology could clearly display anatomical structures,lesions and adjacent organs,improving diagnostic accuracy and guiding the surgical decision-making process.CASE SUMMARY Herein we describe a 68-year-old man diagnosed with digestive tract perforation and acute peritonitis caused by a foreign body of Monopterus albus.The patient pre-sented to the emergency department with complaints of dull abdominal pain,profuse sweating and a pale complexion during work.A Monopterus albus had entered the patient’s body through the anus two hours ago.During hospitalization,the 3D reconstruction technology revealed a perforation of the middle rectum complicated with acute peritonitis and showed a clear and complete Monopterus albus bone morphology in the abdominal and pelvic cavities,with the Monopterus albus biting the mesentery.Laparoscopic examination detected a large(diameter of about 1.5 cm)perforation in the mid-rectum.It could be seen that a Monopterus albus had completely entered the abdominal cavity and had tightly bitten the mesentery of the small intestine.During the operation,the dead Monopterus albus was taken out.CONCLUSION The current manuscript demonstrates that CT is an effective tool for accurate preoperative evaluation of living foreign bodies in clinic.
基金Supported by Hunan Science and Technology Department Planning Project(2012NK3056)
文摘[Objectives]The effect and mechanism of grape seed and sweet wormwood extract on growth of Monopterus albus were studied.[Methods]1 500 healthy M.albus with average mass of 23.8 g/tail were randomly divided into 5 groups.The control group was fed with basal diet and the experimental groups were fed with 10,20,30 and 40 g/kg of grape seed and sweet wormwood extract,respectively.The growth performance of the fish was measured after 60 d of feeding at 23-28℃.[Results]The addition of grape seed and sweet wormwood extract in basal diet could increase the body weight gain rate,specific growth rate and protein efficiency,and decrease the feed coefficient.The activity of digestive enzymes,glutamic-oxaloacetic transaminase,glutamic-pyruvic transaminase,blood alkaline phosphatase and serum total protein was increased,and the contents of blood glucose and triglyceride were decreased.Compared with the control group,the above indexes showed significant difference(P<0.05)when the dosage was 20 and 30 g/kg(P<0.05).The difference was not significant(P>0.05)when the dosage was 10 and 40 g/kg(P>0.05).[Conclusions]As a feed additive,the plant extract from grape seed and sweet wormwood could increase digestive enzyme activity and regulate blood biochemical parameters of fish body.It had the function of promoting the digestion and absorption,enhancing the metabolic activity of 3 main nutrients,and hastening the growth of fish.
文摘Seedlings of the medicinal plant Hyoscyamus albus were supplied with an excess of Cu to examine the possible application in phytoremediation. The seedlings were cultured in B5 medium supplied with basal 0.1 μM Cu and 200 μM Cu under various light conditions: short day (SD);long day (LD);and continuous light (CL). In addition, the effect of supplying 200 μM Cu under Fe deficiency was determined, in order to elucidate the interaction between Cu and Fe. Interestingly, Fe-deficiency symptoms that developed in plants grown with basic levels of Cu under LD almost disappeared when excess Cu was supplied. Plant growth mainly depended on the photo irradiation period (SD < LD^CL);and 200 μM Cu did not inhibit growth at all when Fe was available, whereas in the absence of Fe, CL caused damage to growth. Analysis of the Cu and Fe contents of the plants revealed that Cu was distributed equally in both the aerial parts and roots, whereas most of the Fe was found in the roots;under Fe deficiency, Cu accumulation in the roots apparently increased. Cu was mainly distributed in the soluble fraction, which included vacuoles and the cell-wall fraction. These results provide evidence indicating that H. albus seedlings are tolerant of Cu present in excess. Furthermore, excess Cu was able to compensate for Fe deficiency, depending on the light conditions. Continuous light inhibited this effect, probably as a result of the induction of Mn deficiency. The possible applications of this newly discovered cuprophyte are discussed.
文摘L. albus is an annual grain-legume crop mainly grown for high-protein fodder worldwide but also to produce large seeds for human consumption as a snack-food. In order to make genetic gains in grain yield, assessment of the genetic variation in the germplasm and identification of loci associated with agronomic traits are essential. Phomopsis blight (PB) and Pleiochaeta root rot (PRR), caused by the fungal pathogens Diaporthe toxica and, Pleiochaeta setosa respectively, are two major yield-limiting diseases of the L. albus crop. The extent of genetic diversity in 94 accessions of L. albus comprising: Australian and exotic cultivars, advanced breeding lines, and landraces originating from 26 different countries was determined utilizing PCR-based genic, and microarray-based Diversity Arrays Technology (DArT™), markers. All accessions were evaluated for resistance to PB in two plant tissues (leaves and stems) using either sprayed or injected spore inoculum. A subset of 58 accessions was further evaluated for resistance to PRR by growing seedlings in spore-infested potting mix. The combined data of 724 (50 genic- and 674 DArT) markers were used for cluster analysis. A subset of 324 markers with call rate ≥95% and predicted disease scores of different genotypes were used to identify marker loci accounting for phenotypic variation in PB and PRR resistance using linear regression analysis. Several markers showed significant association with PB or PRR resistance at P < 0.05. Our results showed that favourable alleles for PB and PRR resistance are present in the diverse accessions investigated and they will provide valuable materials for lupin breeding.
文摘The current study has been undertaken to examine the beneficial effect in the power output of a microbial fuel cell (MFC) by adding cellulolytic bacteria Ruminococcus albus (R. albus) into the anodic chamber. Mediator-less H-type MFCs were set up where the anode chamber contained anaerobic digester microorganisms as inocula on finely ground pine tree (Avicel) at 2% (w/v) and the cathode chamber of 10mM phosphate buffered saline conductive solution, both separated by a cation exchange membrane. The functioning of the MFCs for generation of electrical power and the amounts of gaseous byproducts was monitored over a 9-day period. The addition of cellulolytic bacteria caused an increase of average power density from 7.9 m W/m2 to19.5 m W/m2, about 245% increase over a 9-day period. For both groups of MFCs;with R. albus and the control, the head space gases collected were methane and CO2. While the methane: CO2 ratios were found unchanged at 1.7:1 throughout the 9 days of operation, the total gas production increased from 248 mL to 319 mL due to the presence of R. albus addition. This study confirms that whereas the biocatalytic activity of anode microbial population determines the energy production, the addition of external cellulolytic bacteria into anode microbial population can improve and extend the biomass utilization.
文摘[Objective] This study aimed to explore the mutagenesis effects of N＋ ion beam implantation on Streptomyces a/bus and obtain high-yield salinomycin- producing mutant strain. [ Method ] Streptomyces a/bus strain S-11-04 was mutated with different doses of N ＋ implantation. The effects of low energy N ＊ implantation on the survival rate, colony morphology and salinomycin-producing ability were investigated. [ Result] The results showed that low energy N ＋ implantation can efficiently improve the positive mutation rate of Streptomyces albus; 13 mutant strains with high yield of salinomycin were isolated; to be specific, mutant strain N3- 6 has relatively good genetic stability with four continuous generations, and the titres of salinomycin were increased by 41% in the shake-flask culture and 20.5% in mass production compared with the control. [ Conclusion ] N ＋ ion beam irradiation is an effective method to obtain high-yield salinomycin-producing Streptomy- ces albus strain.
基金supported by grants from the National Natural Science Foundation of China(No.31922085)Natural Science Foundation of Hubei Province(Nos.2020CFA056 and 2022BBA0010)Foundation of Hubei Hongshan Laboratory(2022hszd022)to D Luo.
文摘The swamp eel(Monopterus albus)is a commonly cultured freshwater fish.Selection and cultivation of fecund broodstock is crucial for efficient artificial reproduction in this species.However,there are currently no guidelines for selecting high-quality males in M.albus.This study quantitatively investigated the sperm motility of male swamp eels during the breeding season by using computer-assisted sperm analysis system.Variability in the proportion and number of motile sperm was observed between individual males.The testes were grouped into high-quality and low-quality according to sperm quality.Morphometric parameters of the two groups were analyzed.We found that high-quality testes were longer(150±27 mm verses 127±19 mm)than low-quality testes.Besides,the proportion of testes with a dark appearance was slightly higher(58.3%verses 48.1%)in the high-quality group than in the low-quality group.Metabolomic analysis was conducted to compare the metabolite profiles of high-quality and low-quality testes.Glycerophospholipids,organic acids,glycerolipids,amino acids,sphingolipids,and nucleotides were the most abundant metabolites in the testes of swamp eels.Moreover,135 differential metabolites were identified.Several nucleotide derivatives,including 50-deoxyadenosine,20-deoxyadenosine,and ribosyl adenosine,were the most significantly enriched metabolites in highquality testes.These metabolites are potential indicators for the testis quality in M.albus.Altogether,our work provides systematic and quantitative data about the physiological and biochemical characteristics of M.albus testis,which provides an evidential basis for the selection of high-fecundity parents to improve artificial reproduction efficiency of M.albus.
基金supported by grants from the Ministry of Science and Technology of China (2015CB150600)the National Natural Science Foundation of China (31571281 and 31771378)
文摘Dear Editor,Streptomyces can produce a large variety of secondary metabolites as a major source of anti-infective, antitumor or immune-suppressive agents widely applied in clinical treatment. Antibiotics-resistant bacteria are spreading at alarming rates.
基金the Ministry of Education Grant for Ph.D Program，国家重点基础研究发展计划(973计划)
文摘Abstract: A split-root system was used to investigate whether the external or internal P concentration controls root cluster formation and citrate exudation in white lupin (Lupinus albus L.) grown under controlled conditions. In spite of low P concentrations in the shoots and roots of the -P plant, its dry weight was not reduced compared with the +P plant. Supplying external P (0.25 mmol/L) to one root half resulted in an increase in P concentration not only in the shoot, but also in the P-deprived root half, indicating P cycling within the plants. Omitting P from both split-root pots stimulated root cluster formation in both root halves, whereas P supply to one root half stimulated root cluster formation at the beginning of the treatment. Neither P supply to just one root half continuously nor resupply of P to one root half after 19 d of P starvation inhibited root cluster formation on the P-deprived side, although the concentration of P in this root half and shoot increased markedly. The results indicate that root cluster formation in L. albus is controlled by both shoot and root P concentrations. The rates of citrate exudation by both root halves with P deficiency were higher than those of the one root half supplied with P only. In the treatment with one root half supplied with P, the rates of citrate exudation by either the P-supplied or -deprived root halves were almost the same, regardless of P concentration in the roots. The results suggest that internal P concentration controls root cluster formation and citrate exudation in white lupin, but these processes may be regulated by different mechanisms.
文摘IN our previous studies, we have used in situ nick translation techniques for analysis of DNaseⅠ sensitivity and restriction enzymes in situ cleavage on pachytene bivalents of rice-field eels(Monopterus albus Zuiew). A characteristic construction of bivalents--"the lampbrush-likechromosome", which has not been reported yet, was detected in some pachytene bivalents.
文摘为了研究黄鳝(Monopterus albus)热休克蛋白70(Heat Shock Protein 70,简称HSP70)的结构和功能,试验采用生物信息学方法对黄鳝HSP70的基本理化性质、亲/疏水性、信号肽、磷酸化位点、糖基化、无序化特征、二级结构、三级结构、相互作用蛋白网络及进化树进行了研究。结果表明,黄鳝HSP70由441个氨基酸组成,相对分子质量为48146.31,等电点为5.98,整体呈碱性,是较稳定的亲水性蛋白质,有信号肽,但不存在跨膜结构,属于分泌型蛋白质。黄鳝HSP70存在35个潜在磷酸化位点,其中包括19个Ser、14个Thr和2个Tyr磷酸化位点,有5个典型的N糖基化位点。二级结构以α-螺旋(39.68%)、延伸链(20.41%)、β-折叠(6.58%)和无规则卷曲(33.33%)为主要结构。热休克蛋白70(HSP70)作为分子伴侣,在蛋白质折叠和运输、细胞周期调控、细胞凋亡和精子发生等方面发挥着重要作用。研究表明,黄鳝HSP70在抵抗外界应激源、环境应激保护等方面起着重要作用。
基金the National Natural Science Foundation of China(32061143035,32271752)Inner Mongolia Seed Industry Science and Technology Innovation Major Demonstration Project(2022JBGS0040)the Fundamental Research Funds for the Central Universities(lzujbky-2022-ey17).
文摘The plant natural product scopolin,a coumarin secondary metabolite,has been extensively exploited in flavor,cosmetic,medicine,and other industrial fields.Melilotus albus,a leguminous rotation crop,contains high concentrations of coumarin.The transcriptional regulatory network that controls the flow through the scopolin biosynthesis pipeline in M.albus remains poorly understood.MabHLH11 encodes a basic helix–loop–helix(bHLH)transcription factor whose transcription is positively associated with scopolin accumulation and with the expression of MaMYB4,the bHLH partner of the MYB-bHLH complex.Phylogenetic analysis grouped MabHLH11 in the TRANSPARENT TESTA 8(TT8)clade of the bHLH IIIf subgroup.The MabHLH11 protein contained an MYB-interacting region and physically interacted with MaMYB4 in yeast and tobacco leaves.Co-overexpression of MabHLH11 with MaMYB4 in M.albus additively increased the expression of UDP-glucosyltransferase(MaUGT79)and induced more scopolin accumulation than occurred under the expression of MabHLH11 alone.MabHLH11 directly targeted the promoter of MaUGT79 and the activation of MabHLH11 was strengthened by the presence of MaMYB4.Thus,MaMYB4 enhanced the function of MabHLH11 in upregulating scopolin biosynthesis in M.albus,providing a theoretical basis for scalable production of a high-value plant natural product.
