With physical method of micro-gap gas discharge, a large amount of hydroxyl radical can be produced in 20t/h pilot-scale system using the ionization of 02 and H2O. In this paper, the effect of biochemistry of hydroxyl...With physical method of micro-gap gas discharge, a large amount of hydroxyl radical can be produced in 20t/h pilot-scale system using the ionization of 02 and H2O. In this paper, the effect of biochemistry of hydroxyl radicals on introduced organisms in ballast water was experimentally investigated. The results indicate that the contents of chlorophyl-a, chlorophyl-b, chlorophyl-c and carotenoid are decreased by 35%-4% within 8.0s and further to the lowest limit of test 5 minutes. In addition, the main reasons of cell death are the lipid peroxidation, the strong destruction to the monose, amylose, protein, DNA and RNA of cell, and damage in CAT, POD and SOD of antioxidant enzyme system.展开更多
The effect of hydroxyl radical(·OH) on microalgae in ballast water is experimentally investigated. An ?OH plasma generator, which has a thin plate discharge Ag electrode covered by two α-Al2 O3 dielectric layers...The effect of hydroxyl radical(·OH) on microalgae in ballast water is experimentally investigated. An ?OH plasma generator, which has a thin plate discharge Ag electrode covered by two α-Al2 O3 dielectric layers, is built. The plasma generator is filled with O2 and gaseous H2 O and is powered by a homemade power supply. ?OH radicals are generated by a series of plasma reactions and then dissolved into ballast water to kill microalgae. The maximum density of the killed microalgae is about 11 000 cells per milliliter. At this density, the microalgae are not reactivated at the 48th hour and the 120th hour in the treated ballast waters, and the content of chlorophyll in these microalgae decreases by 100%. The water quality returns to a normal level after 120 hours without secondary pollution of aquatic organisms and environment. These results show that the requirements of the D-2 ballast water discharge standard defined by the International Maritime Organization(IMO) are satisfied with the proposed treatment, and that ·OH is an ideal substance for killing microalgae during ballast water replacement of ships.展开更多
The high ratio concentration hydroxyl solution is produced by strong discharge, and applied to the prevention of invasive species in ship ballast water and red tide. This paper introduces the plasma process of hydroxy...The high ratio concentration hydroxyl solution is produced by strong discharge, and applied to the prevention of invasive species in ship ballast water and red tide. This paper introduces the plasma process of hydroxyl radical production, the production process of high ratio hydroxyl solution, and the application to treating ballast water and red tide.展开更多
利用羟基自由基(·OH)压载水处理系统,采用大气压强电场放电技术制取·OH溶液对塔玛亚历山大藻(Alexandrlum tamarense)进行处理。通过普通光学显微镜,荧光显微镜和电子显微镜对·OH处理前后的塔玛亚历山大藻的细胞结构进...利用羟基自由基(·OH)压载水处理系统,采用大气压强电场放电技术制取·OH溶液对塔玛亚历山大藻(Alexandrlum tamarense)进行处理。通过普通光学显微镜,荧光显微镜和电子显微镜对·OH处理前后的塔玛亚历山大藻的细胞结构进行观测。结果表明,·OH能有效破坏藻细胞,从而造成藻类死亡。利用随机扩增多态性DNA(random amplification polymorphic DNA,RAPD)和实时定量PCR(RT-PCR)相结合的技术检测·OH对DNA链的破坏作用。共得到了3条有显著差异的扩增产物。这3条扩增产物经测序,并通过NCBI(national center of biotechnology information)的比对分析,最终得到1条可用RT-PCR检测·OH对DNA破坏作用的基因序列。以上的结果表明,·OH压载水处理系统能有效去除塔玛亚历山大藻,并对其DNA造成破坏。展开更多
基金Key Project of National Foundation Research from Science and Tech-nology, Ministry of China (2002CCC00900), Key and General Projects of NSFC (No. 60371035) and Project of Society Development of Dalian (2004B3SF181).
文摘With physical method of micro-gap gas discharge, a large amount of hydroxyl radical can be produced in 20t/h pilot-scale system using the ionization of 02 and H2O. In this paper, the effect of biochemistry of hydroxyl radicals on introduced organisms in ballast water was experimentally investigated. The results indicate that the contents of chlorophyl-a, chlorophyl-b, chlorophyl-c and carotenoid are decreased by 35%-4% within 8.0s and further to the lowest limit of test 5 minutes. In addition, the main reasons of cell death are the lipid peroxidation, the strong destruction to the monose, amylose, protein, DNA and RNA of cell, and damage in CAT, POD and SOD of antioxidant enzyme system.
基金Project supported by National Natural Science Foundation of China(NSFC)for Distinguished Young Scholar(61025001)National Technology Support Project of China(2013BAC06B00)+1 种基金Special Fund for Marine Scientific Research in the Public Interest(201305027-5)Fundamental Research Fund for the Central Universities(3132013316)
文摘The effect of hydroxyl radical(·OH) on microalgae in ballast water is experimentally investigated. An ?OH plasma generator, which has a thin plate discharge Ag electrode covered by two α-Al2 O3 dielectric layers, is built. The plasma generator is filled with O2 and gaseous H2 O and is powered by a homemade power supply. ?OH radicals are generated by a series of plasma reactions and then dissolved into ballast water to kill microalgae. The maximum density of the killed microalgae is about 11 000 cells per milliliter. At this density, the microalgae are not reactivated at the 48th hour and the 120th hour in the treated ballast waters, and the content of chlorophyll in these microalgae decreases by 100%. The water quality returns to a normal level after 120 hours without secondary pollution of aquatic organisms and environment. These results show that the requirements of the D-2 ballast water discharge standard defined by the International Maritime Organization(IMO) are satisfied with the proposed treatment, and that ·OH is an ideal substance for killing microalgae during ballast water replacement of ships.
基金The study has been funded by Key Project of National Foundation Research from the Ministry of Science and Technology of China,(2002CCC00900),outlay:30,0000Key and General Projects of National Natural Science Foundation of China(NSFC:60371035),outlay:26,0000Key and General Projects of National Natural Science Foundation of China(NSFC:60471036),outlay:21,0000.
文摘The high ratio concentration hydroxyl solution is produced by strong discharge, and applied to the prevention of invasive species in ship ballast water and red tide. This paper introduces the plasma process of hydroxyl radical production, the production process of high ratio hydroxyl solution, and the application to treating ballast water and red tide.
文摘利用羟基自由基(·OH)压载水处理系统,采用大气压强电场放电技术制取·OH溶液对塔玛亚历山大藻(Alexandrlum tamarense)进行处理。通过普通光学显微镜,荧光显微镜和电子显微镜对·OH处理前后的塔玛亚历山大藻的细胞结构进行观测。结果表明,·OH能有效破坏藻细胞,从而造成藻类死亡。利用随机扩增多态性DNA(random amplification polymorphic DNA,RAPD)和实时定量PCR(RT-PCR)相结合的技术检测·OH对DNA链的破坏作用。共得到了3条有显著差异的扩增产物。这3条扩增产物经测序,并通过NCBI(national center of biotechnology information)的比对分析,最终得到1条可用RT-PCR检测·OH对DNA破坏作用的基因序列。以上的结果表明,·OH压载水处理系统能有效去除塔玛亚历山大藻,并对其DNA造成破坏。
