[Objective] This study aimed to investigate the effects of different pretreat- ments on enzymatic saccharification of Miscanthus sinensis and improve reducing sugar yield in the enzymolysis process. [Method] M. sinens...[Objective] This study aimed to investigate the effects of different pretreat- ments on enzymatic saccharification of Miscanthus sinensis and improve reducing sugar yield in the enzymolysis process. [Method] M. sinensis was pretreated with 60Co y-ray irradiation and alkaline hydrogen peroxide, to analyze their effects on re- ducing sugar yield of enzymatic hydrolysis. [Result] After pretreatment with 400 kGy 60Co y-ray irradiation, reducing sugar yield in the enzymolysis process of M sinensis was 76.24 mg/g; after synergic pretreatment with 400 kGy 60Co y-ray irradiation and alkaline hydrogen peroxide, reducing sugar yield in the enzymolysis process of M. sinensis was 505.08 mg/g, which was improved by 5.6 times compared to that in pretreatment with 400 kGy 60Co y-ray irradiation. Based on process optimization, the optimal hydrolysis conditions were obtained： pretreatment temperature 30 ℃, NaOH concentration 1.2%, hydrogen peroxide concentration 2%, pretreatment time 6 h. [Conclusion] Synergic pretreatment with 60Co y-ray irradiation and alkaline hydrogen peroxide could significantly improve reducing sugar yield in the enzymolysis process of M. sinensis, which provided a new theoretical basis for preparing fuel ethanol with M. sinensis.展开更多
In this study,allelochemicals were extracted from pulping effluents rather than from the raw ma-terial of plants.Herein,five organic solvents(ethyl acetate(EAC),methyl tert-butyl ether(MTBE),dichloromethane(DCM),carbo...In this study,allelochemicals were extracted from pulping effluents rather than from the raw ma-terial of plants.Herein,five organic solvents(ethyl acetate(EAC),methyl tert-butyl ether(MTBE),dichloromethane(DCM),carbon tetrachloride(CTC),and petroleum(PE))were applied to sep-arately extracting the allelochemicals from alkaline peroxide mechanical pulp(APMP)effluents.The results from the algal density,inhibition ratio,and optical density of 446 nm(OD 446nm)concluded that the extractives from the APMP effluents can act as effective allelochemicals and showed noticeable allelopathic inhibition effects on Microcystis aeruginosa growth.The results in-dicated that organic solvent extraction could be a practical approach to isolate the allelochemicals from the APMP effluents,which would broaden the potential application of the APMP effluents in the production of antimicrobial agents and other value-added materials.展开更多
基金Supported by National High Technology Research and Development Program of China(863 Program)(2012AA101804)~~
文摘[Objective] This study aimed to investigate the effects of different pretreat- ments on enzymatic saccharification of Miscanthus sinensis and improve reducing sugar yield in the enzymolysis process. [Method] M. sinensis was pretreated with 60Co y-ray irradiation and alkaline hydrogen peroxide, to analyze their effects on re- ducing sugar yield of enzymatic hydrolysis. [Result] After pretreatment with 400 kGy 60Co y-ray irradiation, reducing sugar yield in the enzymolysis process of M sinensis was 76.24 mg/g; after synergic pretreatment with 400 kGy 60Co y-ray irradiation and alkaline hydrogen peroxide, reducing sugar yield in the enzymolysis process of M. sinensis was 505.08 mg/g, which was improved by 5.6 times compared to that in pretreatment with 400 kGy 60Co y-ray irradiation. Based on process optimization, the optimal hydrolysis conditions were obtained： pretreatment temperature 30 ℃, NaOH concentration 1.2%, hydrogen peroxide concentration 2%, pretreatment time 6 h. [Conclusion] Synergic pretreatment with 60Co y-ray irradiation and alkaline hydrogen peroxide could significantly improve reducing sugar yield in the enzymolysis process of M. sinensis, which provided a new theoretical basis for preparing fuel ethanol with M. sinensis.
基金supported by Tianjin Research Program of Application Foundation and Advanced Technology(No.14JCZDJC40500)National Undergraduate Innovation Program(No.2016100570039).
文摘In this study,allelochemicals were extracted from pulping effluents rather than from the raw ma-terial of plants.Herein,five organic solvents(ethyl acetate(EAC),methyl tert-butyl ether(MTBE),dichloromethane(DCM),carbon tetrachloride(CTC),and petroleum(PE))were applied to sep-arately extracting the allelochemicals from alkaline peroxide mechanical pulp(APMP)effluents.The results from the algal density,inhibition ratio,and optical density of 446 nm(OD 446nm)concluded that the extractives from the APMP effluents can act as effective allelochemicals and showed noticeable allelopathic inhibition effects on Microcystis aeruginosa growth.The results in-dicated that organic solvent extraction could be a practical approach to isolate the allelochemicals from the APMP effluents,which would broaden the potential application of the APMP effluents in the production of antimicrobial agents and other value-added materials.
