BACKGROUND Nonalcoholic fatty liver disease(NAFLD)encompasses a variety of liver conditions impacting individuals who consume minimal or no alcohol.Recently,traditional Chinese medicine has been gradually used to trea...BACKGROUND Nonalcoholic fatty liver disease(NAFLD)encompasses a variety of liver conditions impacting individuals who consume minimal or no alcohol.Recently,traditional Chinese medicine has been gradually used to treat mild to moderate fatty liver,among which Dendrobium nobile Lindl.powder has been affirmed by many doctors and patients to be effective.However,there is limited research on combining this treatment with standard therapies for mild to moderate NAFLD.AIM To survey the effect of combining Dendrobium nobile Lindl.powder with standard treatment on liver function and lipid metabolism disorder in patients with mild to moderate NAFLD.METHODS Eighty patients with mild to moderate NAFLD participated in this retrospective study,classified into two groups:The observation group(n=40)and the control group(n=40).In November 2020 and November 2022,the study was conducted at People’s Hospital of Chongqing Liang Jiang New Area.The control group received standard treatment,while the observation group received Dendrobium nobile Lindl.powder based on the control group.The study compared differences in traditional Chinese medicine clinical syndrome scores,liver fibrosis treatment,liver function indicators,lipid levels,and serum inflammatory factor levels before and after treatment,and we calculated the incidence of adverse reactions for both groups.RESULTS The total effective rate was 97.50%in the observation group and 72.5%in the control group.After 8 weeks of treatment,the main and secondary symptom scores remarkably decreased,especially in the observation group(P<0.05),and there was a significant reduction in the serum levels of hyaluronic acid(HA),laminin(LN),human rocollagen III(PC III),and collagen type IV(CIV).The levels of HA,LN,PC III,and CIV were significantly lower in the observation group(P<0.05).After 8 weeks,both groups indicated remarkable improvements in liver function and blood lipid levels,with the observation group having even lower levels(P<0.05).Serum levels of interleukin-1β,tumor necrosis factor-α,and interleukin-8 also dropped significantly.The observation group had a lower rate of adverse reactions(5.00%)compared to the control group(22.50%).CONCLUSION Adding Dendrobium nobile Lindl.powder to standard treatment has been found to remarkably improve symptoms and reduce inflammation in patients with mild to moderate fatty liver disease.It also enhances hepatic function and lipid profile,ameliorates liver fibrosis indices,and lowers the risk of side effects.Consequently,this therapeutic protocol shows promise for clinical implementation and dissemination.展开更多
Dendrobium nobile Lindl.alkaloids（DNLA）,the active ingredients of a traditional Chinese medicine Dendrobium,have been shown to have anti-oxidative effects,anti-inflammatory action,and protective effect on neurons ag...Dendrobium nobile Lindl.alkaloids（DNLA）,the active ingredients of a traditional Chinese medicine Dendrobium,have been shown to have anti-oxidative effects,anti-inflammatory action,and protective effect on neurons against oxygen-glucose deprivation.However,it is not clear whether DNLA reduces amyloid-beta（Aβ）-induced neuronal injury.In this study,cortical neurons were treated with DNLA at different concentrations（0.025,0.25,and 2.5 mg/L）for 24 hours,followed by administration of Aβ（25-35）（10μM）.Aβ（25-35） treatments increased cell injury as determined by the leakage of lactate dehydrogenase,which was accompanied by chromatin condensation and mitochondrial tumefaction.The damage caused by Aβ（25-35） on these cellular properties was markedly attenuated when cells were pretreated with DNLA.Treatment with Aβ（25-35）down-regulated the expressions of postsynaptic density-95 mRNA and decreased the protein expression of synaptophysin and postsynaptic density-95,all changes were significantly reduced by pretreatment of cells with DNLA.These findings suggest that DNLA reduces the cytotoxicity induced by Aβ（25-35） in rat primary cultured neurons.The protective mechanism that DNLA confers on the synaptic integrity of cultured neurons might be mediated,at least in part,through the upregulation of neurogenesis related proteins synaptophysin and postsynaptic density-95.展开更多
Dendrobium nobile is an important medicinal and nutraceutical herb.Although the ingredients of D.nobile have been identified as polysaccharides,alkaloids,amino acids,flavonoids and bibenzyls,our understanding of the m...Dendrobium nobile is an important medicinal and nutraceutical herb.Although the ingredients of D.nobile have been identified as polysaccharides,alkaloids,amino acids,flavonoids and bibenzyls,our understanding of the metabolic pathways that regulate the synthesis of these compounds is limited.Here,we used transcriptomic and metabolic analyses to elucidate the genes and metabolites involved in the biosynthesis of carbohydrate and several secondary metabolites in the stems of D.nobile.A total of 1005 metabolites and 31,745 genes were detected in the stems of D.nobile.The majority of these metabolites and genes were involved in the metabolism of carbohydrates(fructose,mannose,glucose,xylulose and starch),while some were involved in the metabolism of secondary metabolites(alkaloids,β-tyrosine,ferulic acid,4-hydroxybenzoate and chrysin).Our predicted regulatory network indicated that five genes(AROG,PYK,DXS,ACEE and HMGCR) might play vital roles in the transition from carbohydrate to alkaloid synthesis.Correlation analysis identified that six genes(ALDO,PMM,BGLX,EGLC,XYLB and GLGA) were involved in carbohydrate metabolism,and two genes(ADT and CYP73A) were involved in secondary metabolite biosynthesis.Our analyses also indicated that phosphoenol-pyruvate(PEP) was a crucial bridge that connected carbohydrate to alkaloid biosynthesis.The regulatory network between carbohydrate and secondary metabolite biosynthesis established will provide important insights into the regulation of metabolites and biological systems in Dendrobium species.展开更多
OBJECTIVE Activation of the PINK1/Parkin-mediated autophagy pathway has been proposed to play a protective role in the development of neurological disorders through the elimination of damaged macromolecules or organel...OBJECTIVE Activation of the PINK1/Parkin-mediated autophagy pathway has been proposed to play a protective role in the development of neurological disorders through the elimination of damaged macromolecules or organelles.Exposure to excessive manganese(Mn) causes neurotoxicity and can produce a Parkinson disease(PD)-like neurological disorder.Mitochondrial dysfunction and oxidative stress are implicated in the mechanism of Mn induced neurotoxicity.The present study was designed to determine whether Dendrobium nobile Lindl.alkaloids(DNLA),a Chinese medicinal herb extract,confers protective function over Mn-induced cell toxicity,and to investigate whether the modulation of PINK1/Parkin-mediated autophagy is involved in the mechanism of DNLA-mediated cell protection over Mn toxicity.METHODS AND RESULTS Rat adrenal pheochromocytoma PC12 cells were utilized as an in vitro model of Mn cell toxicity.It was found that the treatment of the PC12 cells with Mn resulted in concentration-dependent cell death,accompanied by a decrease in mitochondrial respiration capacity and an increase in ROS generation,whereas pretreatment of cells with DNLA significantly alleviated cell toxicity induced by Mn and improved mitochondrial function and oxidative status.Mn treatment enhanced apoptotic cells along with a marked increase in the protein expression of Bax and a decrease in the expression of Bcl-2 protein.On the contrary,DNLA increased Bcl-2 expression,and concomitantly dramatically decreased the Bax/Bcl-2 ratio.Analysis of the expression of PINK1 and Parkin revealed that pretreatment of cells with DNLA significantly alleviated the decrease in protein levels of both PINK1 and Parkin caused by Mn.Furthermore,cells treated with Mn exhibited increased expression of LC3-Ⅱ and a decrease in accumulation of P62,which was noticeably reversed by the pretreatment of cells with DNLA.CONCLUSION DNLA inhibits Mn induced cytotoxicity,which may be mediated through modulating PINK1/Parkin-mediated autophagic flux and improving mitochondrial function.展开更多
[Objectives]To improve the yield of flavonoids from Dendrobium nobile Lindl.[Methods]On the basis of four single-factor experiments of solid/liquid ratio,extraction temperature,extraction time and ethanol volume fract...[Objectives]To improve the yield of flavonoids from Dendrobium nobile Lindl.[Methods]On the basis of four single-factor experiments of solid/liquid ratio,extraction temperature,extraction time and ethanol volume fraction,the ultrasonic-assisted extraction process of total flavonoids from D.nobile Lindl.was optimized using quadratic general rotary unitized design,and the in-vitro anti-oxidant activity of the flavonoids extracted was evaluated preliminarily.[Results]The optimized extraction process for flavonoids in D.nobile Lindl.was as follows:extraction time 10 min,solid/liquid ratio 1∶15(g∶mL),extraction temperature 75℃and ethanol volume fraction 90%.According to the revised optimal process,6 repeated experiments were carried out,and the average yield of flavonoids was(0.5515±0.0004)mg/g,consistent with the predicted value of the model.The results of anti-oxidation experiment show that the flavonoids extracted from D.nobile Lindl.have better scavenging effect on ABTS and DPPH free radicals,and the scavenging activity is proportional to the mass concentration of the extract.[Conclusions]The optimized extraction process for flavonoids from D.nobile Lindl.is reasonable and feasible,and the flavonoids in D.nobile Lindl.have strong antioxidant activity.展开更多
Dendrobium nobile Lindl (D. nobile), a well-known precious herb, has a long history of use as a medicine and health food in China. Phytochemically, D. nobile has been found to contain various bioactive compounds, such...Dendrobium nobile Lindl (D. nobile), a well-known precious herb, has a long history of use as a medicine and health food in China. Phytochemically, D. nobile has been found to contain various bioactive compounds, such as alkaloids, bibenzyl, phenanthrene, phenylpropanoids, and polysaccharides. Its medicinal applications are closely correlated to its diverse pharmacological activities, including antitumor, anti-inflammatory, nervous system protective, antifatigue, hypoglycemic, and hypolipidemic actions. In this review, we provide a comprehensive summary of the main chemical constituents and pharmacological activities of D. nobile, as well as the underlying molecular mechanisms for its bioactivities. It is expected that this review will provide a helpful scientific reference for the development and use of D. nobile.展开更多
A new dendrobine-type alkaloid has been isolated from stems of Dendrobium nobile lindl.. Its structure has been identified on the basis of spectroscopic method.
中药石斛基原复杂,其主流品种有霍山石斛Dendrobium huoshanense C.Z.Tang et S.J.Cheng、铁皮石斛D.officinale Kimura et Migo、金钗石斛D.nobile Lindl.,《中华人民共和国药典》(以下简称《中国药典》)2005年版石斛项下增加了“及其...中药石斛基原复杂,其主流品种有霍山石斛Dendrobium huoshanense C.Z.Tang et S.J.Cheng、铁皮石斛D.officinale Kimura et Migo、金钗石斛D.nobile Lindl.,《中华人民共和国药典》(以下简称《中国药典》)2005年版石斛项下增加了“及其近似种”的描述,难以确定具体物种,有必要对其进一步正本清源。对石斛属的起源进行本草考证,对霍山石斛、铁皮石斛、金钗石斛的本草起源进行梳理,为不同石斛属药材的应用提供参考。霍山石斛是最早作为中药石斛应用的品种,高效液相色谱法特征图谱等方法为《中国药典》2020年版收录霍山石斛提供了技术支撑;铁皮石斛应为石斛药材中应用时间最久、范围最广的品种,其主要为浙江、云南、贵州,以及广东、广西、福建、江西等地丹霞地貌上生长的种群,应高度重视并对其进行保护;D.nobile在历代文献中的中文名称混乱,应明确将其作为金钗石斛的拉丁学名。枫斗是我国名贵中药和传统保健品,起源于霍山石斛。古代名医对霍山石斛、铁皮石斛、金钗石斛、川石斛的用法各有特色,建议厘清品种来源、功效,加强现代研究,在修订《中国药典》时将其各自单列,充分发挥中药石斛的临床价值。展开更多
取粉碎均匀的石斛样品1 g置于50 m L聚四氟乙烯离心管中,加入0.1 mol·L^(-1)盐酸溶液5 m L,涡旋振荡2 min,充分浸润,加入15 m L乙醇,涡旋振荡1 min,超声30 min,冷却至室温,加入6 g无水硫酸镁和1.5 g无水乙酸钠,振摇3 min,冰浴冷却5...取粉碎均匀的石斛样品1 g置于50 m L聚四氟乙烯离心管中,加入0.1 mol·L^(-1)盐酸溶液5 m L,涡旋振荡2 min,充分浸润,加入15 m L乙醇,涡旋振荡1 min,超声30 min,冷却至室温,加入6 g无水硫酸镁和1.5 g无水乙酸钠,振摇3 min,冰浴冷却5 min,以转速8000 r·min^(-1)离心5 min,取上清液1 m L用乙醇定容至25 m L,采用高效液相色谱-串联质谱法(HPLC-MS/MS)测定石斛碱和毛兰素的含量。以Kinetex F5色谱柱为固定相,以不同体积比的乙醇-0.1%(体积分数)甲酸溶液混合液为流动相进行梯度洗脱,质谱分析采用电喷雾离子(ESI)源,正离子(ESI+)扫描模式,多反应监测(MRM)模式。结果表明,石斛碱和毛兰素的质量浓度在一定范围内与对应的峰面积呈线性关系,检出限(3S/N)分别为0.02,0.003 mg·kg^(-1);按照标准加入法进行回收试验,回收率为93.6%~97.1%,测定值的相对标准偏差(n=6)均小于3.0%;方法用于测定不同栽培方式、不同年限、不同部位金钗石斛中石斛碱、毛兰素的含量,均存在差异。展开更多
【目的】阐明不同基质种植下石斛根系内生菌菌群的差异性,为进一步研究内生菌组成差异对石斛药效成分积累的影响、优化种植条件提供参考依据。【方法】选取仿野生贴石种植于丹霞石块和火山石块的2年生石斛根系,进行16S rRNA V4区域和IT...【目的】阐明不同基质种植下石斛根系内生菌菌群的差异性,为进一步研究内生菌组成差异对石斛药效成分积累的影响、优化种植条件提供参考依据。【方法】选取仿野生贴石种植于丹霞石块和火山石块的2年生石斛根系,进行16S rRNA V4区域和ITS 2区域的高通量测序,分析2种基质种植下石斛根系内生细菌和内生真菌的次生代谢产物、群落组成结构、alpha多样性、beta多样性和功能预测。【结果】火山石与丹霞石的石斛根系次生代谢产物中倍半萜糖苷类物质Dendromoniliside C or isomer(288-p)和Dendromoniliside D or isomer(123-n)差异显著(P<0.05,下同)。群落结构分析显示,内生细菌和内生真菌的组成在门水平和属水平上均无明显差异,其中,内生细菌在门水平上均以变形菌门(Proteobacteria)相对丰度最为富集,在属水平上均以鞘脂单胞菌属(Sphingomonas)相对丰度最为富集;内生真菌在门水平上均以子囊菌门(Ascomycota)为绝对优势门,在属水平上均以暗球腔菌属(Phaeosphaeria)为优势菌群。主成分分析反映火山石与丹霞石样本组间差异明显,基于ANOSIM组间差异检验下的Unweighted UniFrac指数、Bray Curtis指数和Jaccard指数均进一步表明不同基质种植下石斛内生菌的组间差异远大于组内差异。功能预测结果显示,在2种基质下石斛根系中,内生菌真菌营养型以病理腐生营养型(Pathotroph-Saprotroph)和腐生营养型(Saprotroph)差异最显著,在主要功能通路中真菌寄生—植物病原—植物腐生菌功能群(Fungal Parasite-Plant PathogenPlant Saprotroph)和未定义腐生菌功能群(Undefined Saprotroph)差异显著,均为丹霞石显著高于火山石。【结论】2种基质种植环境显著影响石斛根系内生菌群的次级代谢产物、菌群相对丰度、优势菌群和群落功能。同时也提示,石斛根系内生真菌相较于内生细菌,对不同种植基质条件更敏感。展开更多
基金Supported by the Chongqing Science and Health Joint Medical Research Project,No.2022MSXM133the First Batch of Key Disciplines on Public Health in Chongqing,Natural Science Foundation of Chongqing,No.CSTB2022NSCQ-MSX1522.
文摘BACKGROUND Nonalcoholic fatty liver disease(NAFLD)encompasses a variety of liver conditions impacting individuals who consume minimal or no alcohol.Recently,traditional Chinese medicine has been gradually used to treat mild to moderate fatty liver,among which Dendrobium nobile Lindl.powder has been affirmed by many doctors and patients to be effective.However,there is limited research on combining this treatment with standard therapies for mild to moderate NAFLD.AIM To survey the effect of combining Dendrobium nobile Lindl.powder with standard treatment on liver function and lipid metabolism disorder in patients with mild to moderate NAFLD.METHODS Eighty patients with mild to moderate NAFLD participated in this retrospective study,classified into two groups:The observation group(n=40)and the control group(n=40).In November 2020 and November 2022,the study was conducted at People’s Hospital of Chongqing Liang Jiang New Area.The control group received standard treatment,while the observation group received Dendrobium nobile Lindl.powder based on the control group.The study compared differences in traditional Chinese medicine clinical syndrome scores,liver fibrosis treatment,liver function indicators,lipid levels,and serum inflammatory factor levels before and after treatment,and we calculated the incidence of adverse reactions for both groups.RESULTS The total effective rate was 97.50%in the observation group and 72.5%in the control group.After 8 weeks of treatment,the main and secondary symptom scores remarkably decreased,especially in the observation group(P<0.05),and there was a significant reduction in the serum levels of hyaluronic acid(HA),laminin(LN),human rocollagen III(PC III),and collagen type IV(CIV).The levels of HA,LN,PC III,and CIV were significantly lower in the observation group(P<0.05).After 8 weeks,both groups indicated remarkable improvements in liver function and blood lipid levels,with the observation group having even lower levels(P<0.05).Serum levels of interleukin-1β,tumor necrosis factor-α,and interleukin-8 also dropped significantly.The observation group had a lower rate of adverse reactions(5.00%)compared to the control group(22.50%).CONCLUSION Adding Dendrobium nobile Lindl.powder to standard treatment has been found to remarkably improve symptoms and reduce inflammation in patients with mild to moderate fatty liver disease.It also enhances hepatic function and lipid profile,ameliorates liver fibrosis indices,and lowers the risk of side effects.Consequently,this therapeutic protocol shows promise for clinical implementation and dissemination.
基金financially supported by the National Natural Science Foundation of China,No.81473201the Natural Science Foundation of Educational Commission of Guizhou Province of China,No.2010043+1 种基金the Science and Technology Foundation of Guizhou Province of China,No.JZ[2014]2016the Modernization of Traditional Chinese Medicine Project of Guizhou Province of China,No.[2011]5086
文摘Dendrobium nobile Lindl.alkaloids（DNLA）,the active ingredients of a traditional Chinese medicine Dendrobium,have been shown to have anti-oxidative effects,anti-inflammatory action,and protective effect on neurons against oxygen-glucose deprivation.However,it is not clear whether DNLA reduces amyloid-beta（Aβ）-induced neuronal injury.In this study,cortical neurons were treated with DNLA at different concentrations（0.025,0.25,and 2.5 mg/L）for 24 hours,followed by administration of Aβ（25-35）（10μM）.Aβ（25-35） treatments increased cell injury as determined by the leakage of lactate dehydrogenase,which was accompanied by chromatin condensation and mitochondrial tumefaction.The damage caused by Aβ（25-35） on these cellular properties was markedly attenuated when cells were pretreated with DNLA.Treatment with Aβ（25-35）down-regulated the expressions of postsynaptic density-95 mRNA and decreased the protein expression of synaptophysin and postsynaptic density-95,all changes were significantly reduced by pretreatment of cells with DNLA.These findings suggest that DNLA reduces the cytotoxicity induced by Aβ（25-35） in rat primary cultured neurons.The protective mechanism that DNLA confers on the synaptic integrity of cultured neurons might be mediated,at least in part,through the upregulation of neurogenesis related proteins synaptophysin and postsynaptic density-95.
基金supported by the Project for Innovation Team of Yunnan Province (202105AE160012)the Project for Construction of International Flower Technology Innovation Center and Achievement Industrialization (2019ZG006)+2 种基金the Project for the Germplasm Bank of Wild Speciesthe KC Wong Education Foundation,CASthe project for High-level Talent Training Plan of Yunnan Province
文摘Dendrobium nobile is an important medicinal and nutraceutical herb.Although the ingredients of D.nobile have been identified as polysaccharides,alkaloids,amino acids,flavonoids and bibenzyls,our understanding of the metabolic pathways that regulate the synthesis of these compounds is limited.Here,we used transcriptomic and metabolic analyses to elucidate the genes and metabolites involved in the biosynthesis of carbohydrate and several secondary metabolites in the stems of D.nobile.A total of 1005 metabolites and 31,745 genes were detected in the stems of D.nobile.The majority of these metabolites and genes were involved in the metabolism of carbohydrates(fructose,mannose,glucose,xylulose and starch),while some were involved in the metabolism of secondary metabolites(alkaloids,β-tyrosine,ferulic acid,4-hydroxybenzoate and chrysin).Our predicted regulatory network indicated that five genes(AROG,PYK,DXS,ACEE and HMGCR) might play vital roles in the transition from carbohydrate to alkaloid synthesis.Correlation analysis identified that six genes(ALDO,PMM,BGLX,EGLC,XYLB and GLGA) were involved in carbohydrate metabolism,and two genes(ADT and CYP73A) were involved in secondary metabolite biosynthesis.Our analyses also indicated that phosphoenol-pyruvate(PEP) was a crucial bridge that connected carbohydrate to alkaloid biosynthesis.The regulatory network between carbohydrate and secondary metabolite biosynthesis established will provide important insights into the regulation of metabolites and biological systems in Dendrobium species.
文摘OBJECTIVE Activation of the PINK1/Parkin-mediated autophagy pathway has been proposed to play a protective role in the development of neurological disorders through the elimination of damaged macromolecules or organelles.Exposure to excessive manganese(Mn) causes neurotoxicity and can produce a Parkinson disease(PD)-like neurological disorder.Mitochondrial dysfunction and oxidative stress are implicated in the mechanism of Mn induced neurotoxicity.The present study was designed to determine whether Dendrobium nobile Lindl.alkaloids(DNLA),a Chinese medicinal herb extract,confers protective function over Mn-induced cell toxicity,and to investigate whether the modulation of PINK1/Parkin-mediated autophagy is involved in the mechanism of DNLA-mediated cell protection over Mn toxicity.METHODS AND RESULTS Rat adrenal pheochromocytoma PC12 cells were utilized as an in vitro model of Mn cell toxicity.It was found that the treatment of the PC12 cells with Mn resulted in concentration-dependent cell death,accompanied by a decrease in mitochondrial respiration capacity and an increase in ROS generation,whereas pretreatment of cells with DNLA significantly alleviated cell toxicity induced by Mn and improved mitochondrial function and oxidative status.Mn treatment enhanced apoptotic cells along with a marked increase in the protein expression of Bax and a decrease in the expression of Bcl-2 protein.On the contrary,DNLA increased Bcl-2 expression,and concomitantly dramatically decreased the Bax/Bcl-2 ratio.Analysis of the expression of PINK1 and Parkin revealed that pretreatment of cells with DNLA significantly alleviated the decrease in protein levels of both PINK1 and Parkin caused by Mn.Furthermore,cells treated with Mn exhibited increased expression of LC3-Ⅱ and a decrease in accumulation of P62,which was noticeably reversed by the pretreatment of cells with DNLA.CONCLUSION DNLA inhibits Mn induced cytotoxicity,which may be mediated through modulating PINK1/Parkin-mediated autophagic flux and improving mitochondrial function.
基金Key Science and Technology Program of Science&Technology Department of Henan Province(172102310211,182102110166,192102310087,202102310478)Key Scientific and Technological Research Project for Colleges and Universities(18A180026,20B350006)+1 种基金Starting Foundation of Pingdingshan University for High-level Talents(PXY-BSQD-2018011,PXY-BSQD-2018010)Cultivation Fund of Pingdingshan University(PXY-PYJJ-2019007)。
文摘[Objectives]To improve the yield of flavonoids from Dendrobium nobile Lindl.[Methods]On the basis of four single-factor experiments of solid/liquid ratio,extraction temperature,extraction time and ethanol volume fraction,the ultrasonic-assisted extraction process of total flavonoids from D.nobile Lindl.was optimized using quadratic general rotary unitized design,and the in-vitro anti-oxidant activity of the flavonoids extracted was evaluated preliminarily.[Results]The optimized extraction process for flavonoids in D.nobile Lindl.was as follows:extraction time 10 min,solid/liquid ratio 1∶15(g∶mL),extraction temperature 75℃and ethanol volume fraction 90%.According to the revised optimal process,6 repeated experiments were carried out,and the average yield of flavonoids was(0.5515±0.0004)mg/g,consistent with the predicted value of the model.The results of anti-oxidation experiment show that the flavonoids extracted from D.nobile Lindl.have better scavenging effect on ABTS and DPPH free radicals,and the scavenging activity is proportional to the mass concentration of the extract.[Conclusions]The optimized extraction process for flavonoids from D.nobile Lindl.is reasonable and feasible,and the flavonoids in D.nobile Lindl.have strong antioxidant activity.
文摘Dendrobium nobile Lindl (D. nobile), a well-known precious herb, has a long history of use as a medicine and health food in China. Phytochemically, D. nobile has been found to contain various bioactive compounds, such as alkaloids, bibenzyl, phenanthrene, phenylpropanoids, and polysaccharides. Its medicinal applications are closely correlated to its diverse pharmacological activities, including antitumor, anti-inflammatory, nervous system protective, antifatigue, hypoglycemic, and hypolipidemic actions. In this review, we provide a comprehensive summary of the main chemical constituents and pharmacological activities of D. nobile, as well as the underlying molecular mechanisms for its bioactivities. It is expected that this review will provide a helpful scientific reference for the development and use of D. nobile.
文摘A new dendrobine-type alkaloid has been isolated from stems of Dendrobium nobile lindl.. Its structure has been identified on the basis of spectroscopic method.
文摘中药石斛基原复杂,其主流品种有霍山石斛Dendrobium huoshanense C.Z.Tang et S.J.Cheng、铁皮石斛D.officinale Kimura et Migo、金钗石斛D.nobile Lindl.,《中华人民共和国药典》(以下简称《中国药典》)2005年版石斛项下增加了“及其近似种”的描述,难以确定具体物种,有必要对其进一步正本清源。对石斛属的起源进行本草考证,对霍山石斛、铁皮石斛、金钗石斛的本草起源进行梳理,为不同石斛属药材的应用提供参考。霍山石斛是最早作为中药石斛应用的品种,高效液相色谱法特征图谱等方法为《中国药典》2020年版收录霍山石斛提供了技术支撑;铁皮石斛应为石斛药材中应用时间最久、范围最广的品种,其主要为浙江、云南、贵州,以及广东、广西、福建、江西等地丹霞地貌上生长的种群,应高度重视并对其进行保护;D.nobile在历代文献中的中文名称混乱,应明确将其作为金钗石斛的拉丁学名。枫斗是我国名贵中药和传统保健品,起源于霍山石斛。古代名医对霍山石斛、铁皮石斛、金钗石斛、川石斛的用法各有特色,建议厘清品种来源、功效,加强现代研究,在修订《中国药典》时将其各自单列,充分发挥中药石斛的临床价值。
文摘取粉碎均匀的石斛样品1 g置于50 m L聚四氟乙烯离心管中,加入0.1 mol·L^(-1)盐酸溶液5 m L,涡旋振荡2 min,充分浸润,加入15 m L乙醇,涡旋振荡1 min,超声30 min,冷却至室温,加入6 g无水硫酸镁和1.5 g无水乙酸钠,振摇3 min,冰浴冷却5 min,以转速8000 r·min^(-1)离心5 min,取上清液1 m L用乙醇定容至25 m L,采用高效液相色谱-串联质谱法(HPLC-MS/MS)测定石斛碱和毛兰素的含量。以Kinetex F5色谱柱为固定相,以不同体积比的乙醇-0.1%(体积分数)甲酸溶液混合液为流动相进行梯度洗脱,质谱分析采用电喷雾离子(ESI)源,正离子(ESI+)扫描模式,多反应监测(MRM)模式。结果表明,石斛碱和毛兰素的质量浓度在一定范围内与对应的峰面积呈线性关系,检出限(3S/N)分别为0.02,0.003 mg·kg^(-1);按照标准加入法进行回收试验,回收率为93.6%~97.1%,测定值的相对标准偏差(n=6)均小于3.0%;方法用于测定不同栽培方式、不同年限、不同部位金钗石斛中石斛碱、毛兰素的含量,均存在差异。
文摘【目的】阐明不同基质种植下石斛根系内生菌菌群的差异性,为进一步研究内生菌组成差异对石斛药效成分积累的影响、优化种植条件提供参考依据。【方法】选取仿野生贴石种植于丹霞石块和火山石块的2年生石斛根系,进行16S rRNA V4区域和ITS 2区域的高通量测序,分析2种基质种植下石斛根系内生细菌和内生真菌的次生代谢产物、群落组成结构、alpha多样性、beta多样性和功能预测。【结果】火山石与丹霞石的石斛根系次生代谢产物中倍半萜糖苷类物质Dendromoniliside C or isomer(288-p)和Dendromoniliside D or isomer(123-n)差异显著(P<0.05,下同)。群落结构分析显示,内生细菌和内生真菌的组成在门水平和属水平上均无明显差异,其中,内生细菌在门水平上均以变形菌门(Proteobacteria)相对丰度最为富集,在属水平上均以鞘脂单胞菌属(Sphingomonas)相对丰度最为富集;内生真菌在门水平上均以子囊菌门(Ascomycota)为绝对优势门,在属水平上均以暗球腔菌属(Phaeosphaeria)为优势菌群。主成分分析反映火山石与丹霞石样本组间差异明显,基于ANOSIM组间差异检验下的Unweighted UniFrac指数、Bray Curtis指数和Jaccard指数均进一步表明不同基质种植下石斛内生菌的组间差异远大于组内差异。功能预测结果显示,在2种基质下石斛根系中,内生菌真菌营养型以病理腐生营养型(Pathotroph-Saprotroph)和腐生营养型(Saprotroph)差异最显著,在主要功能通路中真菌寄生—植物病原—植物腐生菌功能群(Fungal Parasite-Plant PathogenPlant Saprotroph)和未定义腐生菌功能群(Undefined Saprotroph)差异显著,均为丹霞石显著高于火山石。【结论】2种基质种植环境显著影响石斛根系内生菌群的次级代谢产物、菌群相对丰度、优势菌群和群落功能。同时也提示,石斛根系内生真菌相较于内生细菌,对不同种植基质条件更敏感。
