Objective： Beside patient＇ s history and skin prick testing（SPT） the detection of specific IgE（slgE） represents an important tool of allergy diagnostics. In recent years different technologies for the detection ...Objective： Beside patient＇ s history and skin prick testing（SPT） the detection of specific IgE（slgE） represents an important tool of allergy diagnostics. In recent years different technologies for the detection of slgE have been developed. The objective of this study is the comparison of the ALLERG-O-LIQ with the ImmunoCAW System using seven inhalant and four food allergens. Methods： Sera from patients were collected and tested for slgE to inhalant（dl, d2, d5, i6, el, e5 and m3） and food allergens（fl, f2, f24, f24） by ALLERG-O-LIQ（Dr. Fooke Laboratorien GmbH） and by ImmunoCAP System（Phadia）. Further, samples were also tested for total IgE in both systems. Results： Prevalence of positive test results varied between 0/20（f24） and 11/20（e5） for ALLERG-O-LIQ and between 3/18（f23） and ll/20（dl/d5） for ImmunoCAP. The qualitative agreement between both methods was found between 75%（f24） and 100%（d2） depending on the allergen. Overall qualitative agreement for inhalant（n = 140）, food（n = 78） and all allergens（n = 218） tested was 92.1%（kappa = 0.84）, 83.3%（kappa = 0.58）, 89.0%（kappa = 0.77）, respectively. Sensitivity, specificity, positive predictive value （PPV）, negative predictive value（NPV） and diagnostic efficiency（DE） were found at 88.2%, 95.8%, 95.2%, 89.6%, 92.1%（inhalant allergens）, 62.5%, 92.6%, 78.9%, 84.7%, 83.3%（food allergens） and 81.5%, 94.4%, 91.5%, 87.5%, 89.0%（all allergens）. Conclusion： Good to excellent qualitative agreement between ALLERG-O-LIQ and ImmunoCAP for the detection of specific and total IgE could be observed. The degree of agreement depended on the allergen and was higher in the group of inhalant allergens. The ALLERG-O-LIQ System represents a reliable test for the detection of specific and total IgE.展开更多
目的研究呼出气一氧化氮(FeNO)、血清免疫球蛋白E(IgE)和基质金属蛋白酶9(MMP9)的水平与儿童哮喘急性发作之间的关系,为儿童哮喘的预防及治疗提供依据。方法选取沈阳市妇婴医院于2020年11月至2022年11月收治的98例支气管哮喘急性发作期...目的研究呼出气一氧化氮(FeNO)、血清免疫球蛋白E(IgE)和基质金属蛋白酶9(MMP9)的水平与儿童哮喘急性发作之间的关系,为儿童哮喘的预防及治疗提供依据。方法选取沈阳市妇婴医院于2020年11月至2022年11月收治的98例支气管哮喘急性发作期儿童作为急性组,按照病情程度分成轻度组(n=32)、中度组(n=38)和重度组(n=28),按照2∶1的比例选出49例同期在门诊治疗的处于支气管哮喘缓解期的儿童作为缓解组,随机选取健康体检儿童49例作为健康对照组,分别对他们进行FeNO、MMP9和血清IgE及肺功能[用力肺活量(FVC)、1秒用力呼气量(FEV_(1))、FEV_(1)/FVC%、最大呼气流量(PEF)]检测。应用Pearson相关分析探讨哮喘急性发作期FeNO、MMP9及血清IgE和肺功能之间的联系,并对三者在支气管哮喘急性发作中的预测价值进行分析。结果急性组、缓解组和对照组的年龄、性别、体重指数和病程的比较,差异无统计学意义(P>0.05)。急性组FeNO、MMP9、血清IgE分别为(59.95±12.65)ppb、(4.87±1.44)pg/ml、(330.63±74.88)IU/ml,缓解组分别为(25.23±8.23)ppb、(1.21±0.02)pg/ml、(152.23±32.12)IU/ml,均高于对照组的(12.43±4.09)ppb、(0.53±0.24)pg/ml、(126.34±57.33)IU/ml,差异具有统计学意义(P<0.05)。急性期和缓解期FVC、FEV1、FEV1/FVC%、PEF均低于对照组,差异具有统计学意义(P<0.05)。支气管哮喘急性发作中度组FeNO、MMP9、血清IgE水平分别为(49.23±6.23)ppb、(1.21±0.02)pg/ml、(282.61±59.83)IU/ml,重度组分别为(67.43±10.09)ppb、(0.53±0.24)pg/ml、(356.49±70.82)IU/ml,均高于轻度组的(34.62±10.65)ppb、(4.87±1.44)pg/ml,(189.21±14.33)IU/ml,差异具有统计学意义(P<0.05)。在轻度组中FeNO、MMP9和血清IgE水平均较低,而在中度组中这些指标均较高,其中FVC、FEV_(1)、FEV_(1)/FVC%和PEF均较低,差异具有统计学意义(P<0.05)。FeNO以及MMP9与血清IgE水平呈正相关(P<0.05),FeNO、MMP9以及血清IgE水平与FVC、FEV_(1)、FEV_(1)/FVC%、PEF均呈负相关(P<0.05)。MMP9在支气管哮喘的诊断中表现出了显著的优势,当达到最大约登指数时,对应的截断值为1.17,曲线下面积(Area under curve,AUC)为0.83,敏感度和特异性也分别达到了90.13%和86.5%。结论支气管哮喘急性发作的儿童血清中的FeNO、MMP9以及血清IgE水平显著增高,随肺部功能恶化程度加重而上升,可能与支气管哮喘急性发作患儿肺功能损害程度有关。展开更多
许多过敏原可以介导Ⅰ型超敏反应,通过与IgE特异性结合,引起过敏症状.过敏原与细胞表面的特异性IgE结合的部分叫做表位,其与IgE的结合能力可以表征过敏原致敏性的强弱.Der p 2是一种重要的屋尘螨过敏原,其线性表位中含有的酪氨酸可被空...许多过敏原可以介导Ⅰ型超敏反应,通过与IgE特异性结合,引起过敏症状.过敏原与细胞表面的特异性IgE结合的部分叫做表位,其与IgE的结合能力可以表征过敏原致敏性的强弱.Der p 2是一种重要的屋尘螨过敏原,其线性表位中含有的酪氨酸可被空气中的NO_(2)和O_(3)硝基化,从而影响线性表位与IgE的结合能力.本实验研究了Der p 2的线性表位及其硝基化产物与IgE的结合能力.研究发现,Der p 2的两条表位多肽可以有效地结合IgE,硝基化表位多肽的IgE结合能力显著高于未硝基化的表位多肽,且不同位点的硝基化对于IgE结合能力的增强程度也不同.结果表明,硝基化能够位点特异性地增强Der p 2的致敏性.展开更多
目的:对青岛大学附属医院就诊的儿童过敏原sIgE分布特点、规律进行回顾性研究,为儿童过敏性疾病的诊疗提供科学依据。方法:收集2021年1月~2022年12月在我院就诊的过敏性或疑似过敏性疾病的0~14岁儿童,来进行血清特异性过敏原IgE (sIgE)...目的:对青岛大学附属医院就诊的儿童过敏原sIgE分布特点、规律进行回顾性研究,为儿童过敏性疾病的诊疗提供科学依据。方法:收集2021年1月~2022年12月在我院就诊的过敏性或疑似过敏性疾病的0~14岁儿童,来进行血清特异性过敏原IgE (sIgE)检测。结果:吸入过敏原组1634例,食物过敏原组2086例,吸入过敏原组阳性率为38.2%,排名前3位依次为粉尘螨(40.8%)、户尘螨(39.2%)、链格孢(25.9%);食物过敏原组阳性率为49.8%,排名前3位依次为蛋白(32.2%)、牛奶(24.7%)、小麦(18.4%)。除了粉尘螨、户尘螨与链格孢,其他不同过敏原检测分级阳性样本中sIgE的浓度均以1~3级为主。除了普通豚草外,男女童组间不同种过敏原slgE阳性率均存在差异,且差异均具有统计学意义(均P Objective: Exploring the retrospective study on the distribution characteristics and patterns of allergens in children treated at Qingdao University Affiliated Hospital, in order to provide effective basis for the diagnosis and treatment of allergic diseases in children. Method: Collect 0~14-year-old children with allergic or suspected allergic diseases who visited our hospital from January 2021 to December 2022 for serum specific allergen IgE testing. Result: There were 1634 cases in the inhaled allergen group and 2086 cases in the food allergen group. The positive rate of the inhaled allergen group was 38.2%, and the top three were dust mites (40.8%), household dust mites (39.2%), and Streptomyces (25.9%);The positive rate of the food allergen group was 49.8%, with the top three ranked in sequence being protein (32.2%), milk (24.7%), and wheat (18.4%). Except for dust mites, household dust mites, and Streptomyces, the concentration of sIgE in positive samples with different allergen detection grades is mainly in grades 1~3. Except for ordinary ragweed, there were differences in the positive rates of different allergens SLgE between male and female groups, and the differences were statistically significant (all P < 0.05). The distribution characteristics of slgE allergens vary among different age groups. The positive rate of food allergens slgE in the infant group is higher than that in other age groups. The three common allergens are milk (58%), crab (56%), and protein (53%);Except for dog dander and cat dander, the positive rate of inhaled allergen SLgE in the preschool group was higher than that in the other two age groups. The three common inhaled allergens in the preschool group were household dust mites (56%), German cockroaches (53%), and dust mites (51%), and the differences between the three age groups were statistically significant (all P < 0.01). Conclusion: The distribution of allergens in children is different in terms of gender, age and intensity. By studying the distribution of serum allergens, we can provide more effective prevention and treatment for children.展开更多
基金the Science and Technique Fund(2005B30601014)the Industry Technology and Development Fund(2007B030701007)
文摘Objective： Beside patient＇ s history and skin prick testing（SPT） the detection of specific IgE（slgE） represents an important tool of allergy diagnostics. In recent years different technologies for the detection of slgE have been developed. The objective of this study is the comparison of the ALLERG-O-LIQ with the ImmunoCAW System using seven inhalant and four food allergens. Methods： Sera from patients were collected and tested for slgE to inhalant（dl, d2, d5, i6, el, e5 and m3） and food allergens（fl, f2, f24, f24） by ALLERG-O-LIQ（Dr. Fooke Laboratorien GmbH） and by ImmunoCAP System（Phadia）. Further, samples were also tested for total IgE in both systems. Results： Prevalence of positive test results varied between 0/20（f24） and 11/20（e5） for ALLERG-O-LIQ and between 3/18（f23） and ll/20（dl/d5） for ImmunoCAP. The qualitative agreement between both methods was found between 75%（f24） and 100%（d2） depending on the allergen. Overall qualitative agreement for inhalant（n = 140）, food（n = 78） and all allergens（n = 218） tested was 92.1%（kappa = 0.84）, 83.3%（kappa = 0.58）, 89.0%（kappa = 0.77）, respectively. Sensitivity, specificity, positive predictive value （PPV）, negative predictive value（NPV） and diagnostic efficiency（DE） were found at 88.2%, 95.8%, 95.2%, 89.6%, 92.1%（inhalant allergens）, 62.5%, 92.6%, 78.9%, 84.7%, 83.3%（food allergens） and 81.5%, 94.4%, 91.5%, 87.5%, 89.0%（all allergens）. Conclusion： Good to excellent qualitative agreement between ALLERG-O-LIQ and ImmunoCAP for the detection of specific and total IgE could be observed. The degree of agreement depended on the allergen and was higher in the group of inhalant allergens. The ALLERG-O-LIQ System represents a reliable test for the detection of specific and total IgE.
文摘目的研究呼出气一氧化氮(FeNO)、血清免疫球蛋白E(IgE)和基质金属蛋白酶9(MMP9)的水平与儿童哮喘急性发作之间的关系,为儿童哮喘的预防及治疗提供依据。方法选取沈阳市妇婴医院于2020年11月至2022年11月收治的98例支气管哮喘急性发作期儿童作为急性组,按照病情程度分成轻度组(n=32)、中度组(n=38)和重度组(n=28),按照2∶1的比例选出49例同期在门诊治疗的处于支气管哮喘缓解期的儿童作为缓解组,随机选取健康体检儿童49例作为健康对照组,分别对他们进行FeNO、MMP9和血清IgE及肺功能[用力肺活量(FVC)、1秒用力呼气量(FEV_(1))、FEV_(1)/FVC%、最大呼气流量(PEF)]检测。应用Pearson相关分析探讨哮喘急性发作期FeNO、MMP9及血清IgE和肺功能之间的联系,并对三者在支气管哮喘急性发作中的预测价值进行分析。结果急性组、缓解组和对照组的年龄、性别、体重指数和病程的比较,差异无统计学意义(P>0.05)。急性组FeNO、MMP9、血清IgE分别为(59.95±12.65)ppb、(4.87±1.44)pg/ml、(330.63±74.88)IU/ml,缓解组分别为(25.23±8.23)ppb、(1.21±0.02)pg/ml、(152.23±32.12)IU/ml,均高于对照组的(12.43±4.09)ppb、(0.53±0.24)pg/ml、(126.34±57.33)IU/ml,差异具有统计学意义(P<0.05)。急性期和缓解期FVC、FEV1、FEV1/FVC%、PEF均低于对照组,差异具有统计学意义(P<0.05)。支气管哮喘急性发作中度组FeNO、MMP9、血清IgE水平分别为(49.23±6.23)ppb、(1.21±0.02)pg/ml、(282.61±59.83)IU/ml,重度组分别为(67.43±10.09)ppb、(0.53±0.24)pg/ml、(356.49±70.82)IU/ml,均高于轻度组的(34.62±10.65)ppb、(4.87±1.44)pg/ml,(189.21±14.33)IU/ml,差异具有统计学意义(P<0.05)。在轻度组中FeNO、MMP9和血清IgE水平均较低,而在中度组中这些指标均较高,其中FVC、FEV_(1)、FEV_(1)/FVC%和PEF均较低,差异具有统计学意义(P<0.05)。FeNO以及MMP9与血清IgE水平呈正相关(P<0.05),FeNO、MMP9以及血清IgE水平与FVC、FEV_(1)、FEV_(1)/FVC%、PEF均呈负相关(P<0.05)。MMP9在支气管哮喘的诊断中表现出了显著的优势,当达到最大约登指数时,对应的截断值为1.17,曲线下面积(Area under curve,AUC)为0.83,敏感度和特异性也分别达到了90.13%和86.5%。结论支气管哮喘急性发作的儿童血清中的FeNO、MMP9以及血清IgE水平显著增高,随肺部功能恶化程度加重而上升,可能与支气管哮喘急性发作患儿肺功能损害程度有关。
文摘许多过敏原可以介导Ⅰ型超敏反应,通过与IgE特异性结合,引起过敏症状.过敏原与细胞表面的特异性IgE结合的部分叫做表位,其与IgE的结合能力可以表征过敏原致敏性的强弱.Der p 2是一种重要的屋尘螨过敏原,其线性表位中含有的酪氨酸可被空气中的NO_(2)和O_(3)硝基化,从而影响线性表位与IgE的结合能力.本实验研究了Der p 2的线性表位及其硝基化产物与IgE的结合能力.研究发现,Der p 2的两条表位多肽可以有效地结合IgE,硝基化表位多肽的IgE结合能力显著高于未硝基化的表位多肽,且不同位点的硝基化对于IgE结合能力的增强程度也不同.结果表明,硝基化能够位点特异性地增强Der p 2的致敏性.
文摘目的:对青岛大学附属医院就诊的儿童过敏原sIgE分布特点、规律进行回顾性研究,为儿童过敏性疾病的诊疗提供科学依据。方法:收集2021年1月~2022年12月在我院就诊的过敏性或疑似过敏性疾病的0~14岁儿童,来进行血清特异性过敏原IgE (sIgE)检测。结果:吸入过敏原组1634例,食物过敏原组2086例,吸入过敏原组阳性率为38.2%,排名前3位依次为粉尘螨(40.8%)、户尘螨(39.2%)、链格孢(25.9%);食物过敏原组阳性率为49.8%,排名前3位依次为蛋白(32.2%)、牛奶(24.7%)、小麦(18.4%)。除了粉尘螨、户尘螨与链格孢,其他不同过敏原检测分级阳性样本中sIgE的浓度均以1~3级为主。除了普通豚草外,男女童组间不同种过敏原slgE阳性率均存在差异,且差异均具有统计学意义(均P Objective: Exploring the retrospective study on the distribution characteristics and patterns of allergens in children treated at Qingdao University Affiliated Hospital, in order to provide effective basis for the diagnosis and treatment of allergic diseases in children. Method: Collect 0~14-year-old children with allergic or suspected allergic diseases who visited our hospital from January 2021 to December 2022 for serum specific allergen IgE testing. Result: There were 1634 cases in the inhaled allergen group and 2086 cases in the food allergen group. The positive rate of the inhaled allergen group was 38.2%, and the top three were dust mites (40.8%), household dust mites (39.2%), and Streptomyces (25.9%);The positive rate of the food allergen group was 49.8%, with the top three ranked in sequence being protein (32.2%), milk (24.7%), and wheat (18.4%). Except for dust mites, household dust mites, and Streptomyces, the concentration of sIgE in positive samples with different allergen detection grades is mainly in grades 1~3. Except for ordinary ragweed, there were differences in the positive rates of different allergens SLgE between male and female groups, and the differences were statistically significant (all P < 0.05). The distribution characteristics of slgE allergens vary among different age groups. The positive rate of food allergens slgE in the infant group is higher than that in other age groups. The three common allergens are milk (58%), crab (56%), and protein (53%);Except for dog dander and cat dander, the positive rate of inhaled allergen SLgE in the preschool group was higher than that in the other two age groups. The three common inhaled allergens in the preschool group were household dust mites (56%), German cockroaches (53%), and dust mites (51%), and the differences between the three age groups were statistically significant (all P < 0.01). Conclusion: The distribution of allergens in children is different in terms of gender, age and intensity. By studying the distribution of serum allergens, we can provide more effective prevention and treatment for children.
