Exfoliated deciduous or an extracted healthy adult tooth can be used to harvest,process,and cryogenically preserve dental pulp stem cells.Future stem cell-based regenerative medicine methods could benefit significantl...Exfoliated deciduous or an extracted healthy adult tooth can be used to harvest,process,and cryogenically preserve dental pulp stem cells.Future stem cell-based regenerative medicine methods could benefit significantly from these mesenchymal stem cells.Teeth serve as a substantial source of mesenchymal stem cells,otherwise disposed of as medical waste.Care should be taken to store this treasure trove of stem cells.Collective responsibility of patients,dentists,and physicians is necessary to ensure that this valuable resource is not wasted and that every possible dental pulp stem cell is available for use in the future.The dental pulp stem cells(DPSC)inside teeth represent a significant future source of stem cells for regenerative medicine procedures.This review describes the ontogeny,the laboratory processing and collection,and isolation methods of DPSC.This review also discusses currently available stem cell banking facilities and their potential use in regenerative medicine procedures in dental and general medical applications in the future.展开更多
Engineered functional organs or tissues,created with autologous somatic cells and seeded on biodegradable or hydrogel scaffolds,have been developed for use in individualswith tissue damage suffered fromcongenital diso...Engineered functional organs or tissues,created with autologous somatic cells and seeded on biodegradable or hydrogel scaffolds,have been developed for use in individualswith tissue damage suffered fromcongenital disorders,infection,irradiation,or cancer.However,in those patients,abnormal cells obtained by biopsy fromthe compromised tissue could potentially contaminate the engineered tissues.Thus,an alternative cell source for construction of the neo-organ or functional recovery of the injured or diseased tissues would be useful.Recently,we have found stem cells existing in the urine.These cells are highly expandable,and have self-renewal capacity,paracrine properties,and multi-differentiation potential.As a novel cell source,urine-derived stem cells(USCs)provide advantages for cell therapy and tissue engineering applications in regeneration of various tissues,particularly in the genitourinary tract,because they originate from the urinary tract system.Importantly,USCs can be obtained via a non-invasive,simple,and low-cost approach and induced with high efficiency to differentiate into three dermal cell lineages.展开更多
Adipose-derived stem cells(ADSCs)have promising applications in tissue regeneration.Currently,there are only a few ADSC products that have been approved for clinical use.The clinical application of ADSCs still faces m...Adipose-derived stem cells(ADSCs)have promising applications in tissue regeneration.Currently,there are only a few ADSC products that have been approved for clinical use.The clinical application of ADSCs still faces many challenges.Here,we review emerging strategies to improve the therapeutic efficacy of ADSCs in tissue regeneration.First,a great quantity of cells is often needed for the stem cell therapies,which requires the advanced cell expansion technologies.In addition cell-derived products are also required for the development of‘cell-free’therapies to overcome the drawbacks of cell-based therapies.Second,it is necessary to strengthen the regenerative functions of ADSCs,including viability,differentiation and paracrine ability,for the tissue repair and regeneration required for different physiological and pathophysiological conditions.Third,poor delivery efficiency also restricts the therapeutic effect of ADSCs.Effective methods to improve cell delivery include alleviating harsh microenvironments,enhancing targeting ability and prolonging cell retention.Moreover,we also point out some critical issues about the sources,effectiveness and safety of ADSCs.With these advanced strategies to improve the therapeutic efficacy of ADSCs,ADSC-based treatment holds great promise for clinical applications in tissue regeneration.展开更多
目的通过文献计量学的方法对当前牙周组织工程干细胞疗法领域的研究现状、热点及发展趋势进行分析。方法在Web of Science核心数据库中检索并筛选了从建库至2021年12月31日干细胞疗法治疗牙周组织缺损的动物实验及临床研究相关文献,使用...目的通过文献计量学的方法对当前牙周组织工程干细胞疗法领域的研究现状、热点及发展趋势进行分析。方法在Web of Science核心数据库中检索并筛选了从建库至2021年12月31日干细胞疗法治疗牙周组织缺损的动物实验及临床研究相关文献,使用R语言的“Bibliometrix”包对文献数据进行文献计量学及可视化分析。结果共纳入304篇文献,文献发表量及被引频次整体呈上升趋势;中国的相关研究以166篇的数量居于全球第一,发文量最高的机构是第四军医大学,发文量最高的作者是Jin Y,发表相关文献最多的期刊是Tissue Engineering Part A;牙周组织工程干细胞疗法的热点主要集中于组织工程及牙周膜再生,前沿主要集中于外泌体、金纳米粒子及血管生成。结论随着牙周组织工程干细胞疗法的研究不断深入,其学术影响力逐渐增高,未来的研究方向可重点关注牙周膜再生、外泌体、金纳米粒子及血管生成等。展开更多
文摘Exfoliated deciduous or an extracted healthy adult tooth can be used to harvest,process,and cryogenically preserve dental pulp stem cells.Future stem cell-based regenerative medicine methods could benefit significantly from these mesenchymal stem cells.Teeth serve as a substantial source of mesenchymal stem cells,otherwise disposed of as medical waste.Care should be taken to store this treasure trove of stem cells.Collective responsibility of patients,dentists,and physicians is necessary to ensure that this valuable resource is not wasted and that every possible dental pulp stem cell is available for use in the future.The dental pulp stem cells(DPSC)inside teeth represent a significant future source of stem cells for regenerative medicine procedures.This review describes the ontogeny,the laboratory processing and collection,and isolation methods of DPSC.This review also discusses currently available stem cell banking facilities and their potential use in regenerative medicine procedures in dental and general medical applications in the future.
基金The authors acknowledge funding support from NIH grant U01CA166886(X.Zhou)National Natural Science Foundation of China(No.81100415)and(No.81371704)+1 种基金Chongqing Natural Science Foundation of Committee of Science and Technology(No.CSTC,2010BB5377)Doctoral Program of the Ministry of Education(No.20115503120009).
文摘Engineered functional organs or tissues,created with autologous somatic cells and seeded on biodegradable or hydrogel scaffolds,have been developed for use in individualswith tissue damage suffered fromcongenital disorders,infection,irradiation,or cancer.However,in those patients,abnormal cells obtained by biopsy fromthe compromised tissue could potentially contaminate the engineered tissues.Thus,an alternative cell source for construction of the neo-organ or functional recovery of the injured or diseased tissues would be useful.Recently,we have found stem cells existing in the urine.These cells are highly expandable,and have self-renewal capacity,paracrine properties,and multi-differentiation potential.As a novel cell source,urine-derived stem cells(USCs)provide advantages for cell therapy and tissue engineering applications in regeneration of various tissues,particularly in the genitourinary tract,because they originate from the urinary tract system.Importantly,USCs can be obtained via a non-invasive,simple,and low-cost approach and induced with high efficiency to differentiate into three dermal cell lineages.
基金supported by National Key Research and Development Program of China(2021YFF1200800,MLG)Sichuan Innovative Research Team Program for Young Scien-tists(2021JDTD0001,MLG)+1 种基金Post-Doctor Research Project,West China Hospital,Sichuan University(2020HXBH025,XY)1·3·5 project for disciplines of excellence,West China Hospital,Sichuan University(ZYJC18017,MLG,ZYJC21079,CP).Conflict of interest。
文摘Adipose-derived stem cells(ADSCs)have promising applications in tissue regeneration.Currently,there are only a few ADSC products that have been approved for clinical use.The clinical application of ADSCs still faces many challenges.Here,we review emerging strategies to improve the therapeutic efficacy of ADSCs in tissue regeneration.First,a great quantity of cells is often needed for the stem cell therapies,which requires the advanced cell expansion technologies.In addition cell-derived products are also required for the development of‘cell-free’therapies to overcome the drawbacks of cell-based therapies.Second,it is necessary to strengthen the regenerative functions of ADSCs,including viability,differentiation and paracrine ability,for the tissue repair and regeneration required for different physiological and pathophysiological conditions.Third,poor delivery efficiency also restricts the therapeutic effect of ADSCs.Effective methods to improve cell delivery include alleviating harsh microenvironments,enhancing targeting ability and prolonging cell retention.Moreover,we also point out some critical issues about the sources,effectiveness and safety of ADSCs.With these advanced strategies to improve the therapeutic efficacy of ADSCs,ADSC-based treatment holds great promise for clinical applications in tissue regeneration.
文摘目的通过文献计量学的方法对当前牙周组织工程干细胞疗法领域的研究现状、热点及发展趋势进行分析。方法在Web of Science核心数据库中检索并筛选了从建库至2021年12月31日干细胞疗法治疗牙周组织缺损的动物实验及临床研究相关文献,使用R语言的“Bibliometrix”包对文献数据进行文献计量学及可视化分析。结果共纳入304篇文献,文献发表量及被引频次整体呈上升趋势;中国的相关研究以166篇的数量居于全球第一,发文量最高的机构是第四军医大学,发文量最高的作者是Jin Y,发表相关文献最多的期刊是Tissue Engineering Part A;牙周组织工程干细胞疗法的热点主要集中于组织工程及牙周膜再生,前沿主要集中于外泌体、金纳米粒子及血管生成。结论随着牙周组织工程干细胞疗法的研究不断深入,其学术影响力逐渐增高,未来的研究方向可重点关注牙周膜再生、外泌体、金纳米粒子及血管生成等。
