Introduction: Perinatal mortality linked to fetal anemia of red cell alloimmunization in Côte d’Ivoire as in many developing countries can be explained by a lack of knowledge of Rhesus D feto-maternal alloimmuni...Introduction: Perinatal mortality linked to fetal anemia of red cell alloimmunization in Côte d’Ivoire as in many developing countries can be explained by a lack of knowledge of Rhesus D feto-maternal alloimmunization, hence the obvious importance of carrying out a study on the problem of prevention of alloimmunization in rhesus negative births. This study takes stock of the management of this pathology in a reference hospital. Results and Discussion: Rh-negative mothers account for 6% of births, 42.7% (70/164) of women had a history of risk, about 61% of women had not had proper prophylaxis during previous pregnancies;and as many had not had follow-up of the coombs test during the current pregnancy;only 4.9% of patients had systematic prophylaxis with anti D serum at 28 weeks of pregnancy. This low rate of prevention is related to the financial difficulties of the patients, but also to a lack of knowledge of the pathology by the nursing staff. Conclusion: Good management of rhesus negative women during their pregnancy allows their incompatible child to benefit from all current treatments ensuring a healthy birth. It is therefore important for medical personnel to know how to deal with this rare disease in a small proportion of pregnant women.展开更多
目的探讨采用黄芩苷预治疗对红细胞免疫的预防性效果。方法采用人类红细胞与佐剂脂多糖(LPS)联合输注C57BL/6小鼠建立红细胞输注模型。设立(1)红细胞输注模型组;(2)未处理对照组:仅注射PBS;(3)黄芩苷预治疗组:在红细胞输注模型建立前一...目的探讨采用黄芩苷预治疗对红细胞免疫的预防性效果。方法采用人类红细胞与佐剂脂多糖(LPS)联合输注C57BL/6小鼠建立红细胞输注模型。设立(1)红细胞输注模型组;(2)未处理对照组:仅注射PBS;(3)黄芩苷预治疗组:在红细胞输注模型建立前一周每天给予小鼠黄芩苷(250 mg/(kg·day))治疗;(4)地塞米松(DEX)对照组:在红细胞输注模型建立前一周每天给予地塞米松(5 mg/(kg·day))治疗。通过流式细胞术检测小鼠血清中IgG和IgM的表达量,并监测小鼠脾脏中的CD4 T细胞和B细胞变化。结果与模型组相比,黄芩苷预治疗能显著降低血清IgG(24823.0±1452.2 vs 15180.0±1172.5,P<0.05)和IgM(194206±2868.6 vs 112287±9741.5,P<0.05)的生成。与模型组相比,黄芩苷预处理组小鼠脾脏中CD25表达明显下降(1006.0±206.1 vs 548.4±19.1,P<0.05),B细胞比例显著降低(31.16%±6.06%vs 15.69%±4.59%,P<0.05),脾脏大小无显著差异[平均长度:(10±0.5)mm vs(12±0.5)mm,P>0.05]。结论黄芩苷能够抑制红细胞免疫,表现为抑制小鼠血清中IgG和IgM的生成,同时抑制脾脏中B细胞增殖和降低CD4 T细胞活化。展开更多
Alloimmunization was combined with lympho-kine activated killer (LAK) cells to assess its effect on mammary carcinoma in rats. The animals were injected with both irradiated allosplenocytes and syngeneic LAK cells. Me...Alloimmunization was combined with lympho-kine activated killer (LAK) cells to assess its effect on mammary carcinoma in rats. The animals were injected with both irradiated allosplenocytes and syngeneic LAK cells. Metastatic lung nodules were markedly reduced using combined therapy when compared with the transfer of LAK cells or alloimmuni-zation alone. IL-2 activity in the serum of alloim-munized rats could be detected. This activity, maintained in vivo for one week, may be responsible for enhancing the antitumor effect of transferred LAK cells.展开更多
Platelet transfusion therapy has allowed successful prevention and treatment of hemorrhagic complications associated with thrombocytopenia since its first use in the 1960's.Consequently, the use of platelets has i...Platelet transfusion therapy has allowed successful prevention and treatment of hemorrhagic complications associated with thrombocytopenia since its first use in the 1960's.Consequently, the use of platelets has increased at an exponential rate.Since展开更多
文摘Introduction: Perinatal mortality linked to fetal anemia of red cell alloimmunization in Côte d’Ivoire as in many developing countries can be explained by a lack of knowledge of Rhesus D feto-maternal alloimmunization, hence the obvious importance of carrying out a study on the problem of prevention of alloimmunization in rhesus negative births. This study takes stock of the management of this pathology in a reference hospital. Results and Discussion: Rh-negative mothers account for 6% of births, 42.7% (70/164) of women had a history of risk, about 61% of women had not had proper prophylaxis during previous pregnancies;and as many had not had follow-up of the coombs test during the current pregnancy;only 4.9% of patients had systematic prophylaxis with anti D serum at 28 weeks of pregnancy. This low rate of prevention is related to the financial difficulties of the patients, but also to a lack of knowledge of the pathology by the nursing staff. Conclusion: Good management of rhesus negative women during their pregnancy allows their incompatible child to benefit from all current treatments ensuring a healthy birth. It is therefore important for medical personnel to know how to deal with this rare disease in a small proportion of pregnant women.
文摘目的探讨采用黄芩苷预治疗对红细胞免疫的预防性效果。方法采用人类红细胞与佐剂脂多糖(LPS)联合输注C57BL/6小鼠建立红细胞输注模型。设立(1)红细胞输注模型组;(2)未处理对照组:仅注射PBS;(3)黄芩苷预治疗组:在红细胞输注模型建立前一周每天给予小鼠黄芩苷(250 mg/(kg·day))治疗;(4)地塞米松(DEX)对照组:在红细胞输注模型建立前一周每天给予地塞米松(5 mg/(kg·day))治疗。通过流式细胞术检测小鼠血清中IgG和IgM的表达量,并监测小鼠脾脏中的CD4 T细胞和B细胞变化。结果与模型组相比,黄芩苷预治疗能显著降低血清IgG(24823.0±1452.2 vs 15180.0±1172.5,P<0.05)和IgM(194206±2868.6 vs 112287±9741.5,P<0.05)的生成。与模型组相比,黄芩苷预处理组小鼠脾脏中CD25表达明显下降(1006.0±206.1 vs 548.4±19.1,P<0.05),B细胞比例显著降低(31.16%±6.06%vs 15.69%±4.59%,P<0.05),脾脏大小无显著差异[平均长度:(10±0.5)mm vs(12±0.5)mm,P>0.05]。结论黄芩苷能够抑制红细胞免疫,表现为抑制小鼠血清中IgG和IgM的生成,同时抑制脾脏中B细胞增殖和降低CD4 T细胞活化。
文摘Alloimmunization was combined with lympho-kine activated killer (LAK) cells to assess its effect on mammary carcinoma in rats. The animals were injected with both irradiated allosplenocytes and syngeneic LAK cells. Metastatic lung nodules were markedly reduced using combined therapy when compared with the transfer of LAK cells or alloimmuni-zation alone. IL-2 activity in the serum of alloim-munized rats could be detected. This activity, maintained in vivo for one week, may be responsible for enhancing the antitumor effect of transferred LAK cells.
文摘Platelet transfusion therapy has allowed successful prevention and treatment of hemorrhagic complications associated with thrombocytopenia since its first use in the 1960's.Consequently, the use of platelets has increased at an exponential rate.Since
