Objective: To examine the anti-hyperglycemic effects of aqueous Lenzites betulina(L. betulina) extracts on normoglycemic glucose-loaded mice.Methods: Different doses of aqueous extract from L. betulina were administer...Objective: To examine the anti-hyperglycemic effects of aqueous Lenzites betulina(L. betulina) extracts on normoglycemic glucose-loaded mice.Methods: Different doses of aqueous extract from L. betulina were administered to 45 ICR mice(Mus musculus) to determine whether there was an effect of L. betulina extracts on the blood glucose level of the ICR mice. Aqueous extracts of L. betulina were orally gavaged to mice using oral glucose tolerance test. A total of five groups were used to determine the effect of the fungi on blood glucose of the mice. Group A(positive control)was given 16.7 mg/kg glimepiride; Group B(negative control) was given distilled water;Group C(low dosage) was given 200 mg/kg aqueous extract; Group D(mid dosage) was given 400 mg/kg aqueous extract and Group E(high dosage) was given 800 mg/kg aqueous extract. Baseline blood glucose value was firstly acquired before induction of hyperglycemia through D-glucose, after which another check on blood glucose was made after 0.5 h. Immediately, after the acquisition of hyperglycemic blood glucose level, the individual administration of treatments were done. After that, three blood collections were done spanning 3 h with 1 h interval.Results: The low dose(200 mg/kg) and the mid dose(400 mg/kg) of L. betulina extracts were significantly different(P < 0.05) from their respective baseline values throughout the whole experiment with the latter surpassing its baseline value during the 3rd hour. On the other hand, the high dose(800 mg/kg) during the 1st hour after administration was not significantly different(P > 0.05) from its corresponding baseline value, acting faster than the positive control(glimepiride), which only became significantly different(P < 0.05) at the 2nd hour.Conclusions: Aqueous L. betulina extract is able to produce hypoglycemic effects on the mice with all doses, which are able to normalize blood glucose levels at varying times.展开更多
Streptozotocin (STZ)-induced diabetic mice increased levels of serum glucose, triglyceride and cholesterol, and decreased level of serum insulin. Effects of Bofutsushosan (BOF: Pulvis ledebouriellae compositae: 防風...Streptozotocin (STZ)-induced diabetic mice increased levels of serum glucose, triglyceride and cholesterol, and decreased level of serum insulin. Effects of Bofutsushosan (BOF: Pulvis ledebouriellae compositae: 防風通聖散) and its composed crude drug, gardeniae fructus (GF: 山梔子) were investigated on levels of these diabetic parameters (serum glucose, insulin, triglyceride and cholesterol) in STZ-diabetic mice. BOF and GF were extracted in 10 volumes of distilled water with an automatic extractor “Torobi”. STZ-induced diabetic mice with serum glucose level of over 600 mg/dl at 3 - 4 weeks after intravenous injection of 150 mg/kg STZ were used for experiments. BOF extract, GF extract, geniposide (a main constituent of GF), and glibenclamide were administered intraperitoneally into 3-hour-fasted STZ-diabetic mice. At 6 hours after administration, BOF extract (100 - 300 mg/kg) decreased high levels of serum glucose, triglyceride and cholesterol, and also increased low level of serum insulin in STZ-diabetic mice in a dose-dependent manner, respectively. Anti-diabetic drug glibenclamide (0.3 - 1 mg/kg) as positive control significantly decreased serum glucose and cholesterol levels, and increased serum insulin level in the diabetic mice. GF extract (30 - 300 mg/kg) decreased serum glucose, triglyceride and cholesterol levels but did not affect serum insulin level in the diabetic mice. Geniposide (10 - 100 mg/kg), decreased serum glucose level but did not affect serum insulin and triglyceride levels in the diabetic mice. These results demonstrated that intraperitoneally administrated BOF extract improved abnormal levels of serum glucose, insulin, triglyceride and cholesterol in the STZ-diabetic mice as being similar to glibenclamide. GF extract has an important role in a part of improving actions of BOF in the diabetic mice. The action of GF extract on serum glucose was parallel with the action of geniposide in the diabetic mice, supporting roles of geniposide in anti-hyperglycemic action of GF.展开更多
注射用艾塞那肽-人血清白蛋白融合蛋白(injectable recombinant protein of exendin-4 and human serum albumin,E2HSA)是我国自主研发的一种长效胰高血糖素样肽-1(glucagon-like peptide-1,GLP-1)受体激动剂,目前处于临床研究阶段。本...注射用艾塞那肽-人血清白蛋白融合蛋白(injectable recombinant protein of exendin-4 and human serum albumin,E2HSA)是我国自主研发的一种长效胰高血糖素样肽-1(glucagon-like peptide-1,GLP-1)受体激动剂,目前处于临床研究阶段。本研究在细胞和整体动物水平研究E2HSA对胰岛β细胞的保护作用。在细胞水平,采用小鼠胰岛素瘤细胞株NIT-1考察E2HSA对细胞活力和增殖的影响;采用水溶性胆固醇诱导NIT-1细胞损伤,考察E2HSA对细胞活力和凋亡的影响,并研究其相关作用机制。在整体动物水平,采用四氧嘧啶高血糖小鼠考察长期注射E2HSA对血糖、血胰岛素和胰岛内胰岛素含量的影响。动物实验操作和福利均遵循中国医学科学院、北京协和医学院药物研究所实验动物伦理与动物福利委员会的规定。结果显示,E2HSA可明显增加NIT-1细胞的活力和掺入的溴脱氧尿嘧啶核苷(bromodeoxyuridine,BrdU);同时显著增加水溶性胆固醇诱导NIT-1细胞损伤后的细胞活力,减少其凋亡发生率,并增加胞内胰腺-十二指肠同源框1(pancreatic duodenal homeobox-1,PDX-1)和蛋白激酶B(protein kinase B,PKB)蛋白的表达。E2HSA长期注射可明显降低四氧嘧啶小鼠的空腹血糖和非禁食血糖,增加其血清和胰岛内的胰岛素含量。综上,E2HSA可以促进NIT-1细胞增殖,抑制水溶性胆固醇诱导的细胞损伤和凋亡,同时增加四氧嘧啶高血糖小鼠血清和胰岛内的胰岛素水平,提示E2HSA可改善小鼠胰岛β细胞功能。展开更多
基金Supported by the University Research Coordination Office of De La Salle University with Challenge Grant No.500-033
文摘Objective: To examine the anti-hyperglycemic effects of aqueous Lenzites betulina(L. betulina) extracts on normoglycemic glucose-loaded mice.Methods: Different doses of aqueous extract from L. betulina were administered to 45 ICR mice(Mus musculus) to determine whether there was an effect of L. betulina extracts on the blood glucose level of the ICR mice. Aqueous extracts of L. betulina were orally gavaged to mice using oral glucose tolerance test. A total of five groups were used to determine the effect of the fungi on blood glucose of the mice. Group A(positive control)was given 16.7 mg/kg glimepiride; Group B(negative control) was given distilled water;Group C(low dosage) was given 200 mg/kg aqueous extract; Group D(mid dosage) was given 400 mg/kg aqueous extract and Group E(high dosage) was given 800 mg/kg aqueous extract. Baseline blood glucose value was firstly acquired before induction of hyperglycemia through D-glucose, after which another check on blood glucose was made after 0.5 h. Immediately, after the acquisition of hyperglycemic blood glucose level, the individual administration of treatments were done. After that, three blood collections were done spanning 3 h with 1 h interval.Results: The low dose(200 mg/kg) and the mid dose(400 mg/kg) of L. betulina extracts were significantly different(P < 0.05) from their respective baseline values throughout the whole experiment with the latter surpassing its baseline value during the 3rd hour. On the other hand, the high dose(800 mg/kg) during the 1st hour after administration was not significantly different(P > 0.05) from its corresponding baseline value, acting faster than the positive control(glimepiride), which only became significantly different(P < 0.05) at the 2nd hour.Conclusions: Aqueous L. betulina extract is able to produce hypoglycemic effects on the mice with all doses, which are able to normalize blood glucose levels at varying times.
文摘Streptozotocin (STZ)-induced diabetic mice increased levels of serum glucose, triglyceride and cholesterol, and decreased level of serum insulin. Effects of Bofutsushosan (BOF: Pulvis ledebouriellae compositae: 防風通聖散) and its composed crude drug, gardeniae fructus (GF: 山梔子) were investigated on levels of these diabetic parameters (serum glucose, insulin, triglyceride and cholesterol) in STZ-diabetic mice. BOF and GF were extracted in 10 volumes of distilled water with an automatic extractor “Torobi”. STZ-induced diabetic mice with serum glucose level of over 600 mg/dl at 3 - 4 weeks after intravenous injection of 150 mg/kg STZ were used for experiments. BOF extract, GF extract, geniposide (a main constituent of GF), and glibenclamide were administered intraperitoneally into 3-hour-fasted STZ-diabetic mice. At 6 hours after administration, BOF extract (100 - 300 mg/kg) decreased high levels of serum glucose, triglyceride and cholesterol, and also increased low level of serum insulin in STZ-diabetic mice in a dose-dependent manner, respectively. Anti-diabetic drug glibenclamide (0.3 - 1 mg/kg) as positive control significantly decreased serum glucose and cholesterol levels, and increased serum insulin level in the diabetic mice. GF extract (30 - 300 mg/kg) decreased serum glucose, triglyceride and cholesterol levels but did not affect serum insulin level in the diabetic mice. Geniposide (10 - 100 mg/kg), decreased serum glucose level but did not affect serum insulin and triglyceride levels in the diabetic mice. These results demonstrated that intraperitoneally administrated BOF extract improved abnormal levels of serum glucose, insulin, triglyceride and cholesterol in the STZ-diabetic mice as being similar to glibenclamide. GF extract has an important role in a part of improving actions of BOF in the diabetic mice. The action of GF extract on serum glucose was parallel with the action of geniposide in the diabetic mice, supporting roles of geniposide in anti-hyperglycemic action of GF.
文摘注射用艾塞那肽-人血清白蛋白融合蛋白(injectable recombinant protein of exendin-4 and human serum albumin,E2HSA)是我国自主研发的一种长效胰高血糖素样肽-1(glucagon-like peptide-1,GLP-1)受体激动剂,目前处于临床研究阶段。本研究在细胞和整体动物水平研究E2HSA对胰岛β细胞的保护作用。在细胞水平,采用小鼠胰岛素瘤细胞株NIT-1考察E2HSA对细胞活力和增殖的影响;采用水溶性胆固醇诱导NIT-1细胞损伤,考察E2HSA对细胞活力和凋亡的影响,并研究其相关作用机制。在整体动物水平,采用四氧嘧啶高血糖小鼠考察长期注射E2HSA对血糖、血胰岛素和胰岛内胰岛素含量的影响。动物实验操作和福利均遵循中国医学科学院、北京协和医学院药物研究所实验动物伦理与动物福利委员会的规定。结果显示,E2HSA可明显增加NIT-1细胞的活力和掺入的溴脱氧尿嘧啶核苷(bromodeoxyuridine,BrdU);同时显著增加水溶性胆固醇诱导NIT-1细胞损伤后的细胞活力,减少其凋亡发生率,并增加胞内胰腺-十二指肠同源框1(pancreatic duodenal homeobox-1,PDX-1)和蛋白激酶B(protein kinase B,PKB)蛋白的表达。E2HSA长期注射可明显降低四氧嘧啶小鼠的空腹血糖和非禁食血糖,增加其血清和胰岛内的胰岛素含量。综上,E2HSA可以促进NIT-1细胞增殖,抑制水溶性胆固醇诱导的细胞损伤和凋亡,同时增加四氧嘧啶高血糖小鼠血清和胰岛内的胰岛素水平,提示E2HSA可改善小鼠胰岛β细胞功能。
