Thenoggin gene is present in the central nervous system in embryonic and postnatal mammals, and plays an important role in maintaining nervous system development and physiological function A 0.76-kb sequence of human ...Thenoggin gene is present in the central nervous system in embryonic and postnatal mammals, and plays an important role in maintaining nervous system development and physiological function A 0.76-kb sequence of human noggin gene was cloned by polymerase chain reaction with the digestion site of Hind Ill and Xba I on the 5＇ end. The cloned fragment was reversely inserted into pCS2＋[Tal]-GFP plasmid, an neural cell-specific antisense eukaryotic expression vector. The plasmid expresses antisense for human noggin specifically in neurons, which may facilitate understanding of the physiological function of noggin.展开更多
基金the Science and Technology Development Program of Shandong Province,No.2007GG10002017the Natural Science Foundation of Shandong Province,No.Y2008C63
文摘Thenoggin gene is present in the central nervous system in embryonic and postnatal mammals, and plays an important role in maintaining nervous system development and physiological function A 0.76-kb sequence of human noggin gene was cloned by polymerase chain reaction with the digestion site of Hind Ill and Xba I on the 5＇ end. The cloned fragment was reversely inserted into pCS2＋[Tal]-GFP plasmid, an neural cell-specific antisense eukaryotic expression vector. The plasmid expresses antisense for human noggin specifically in neurons, which may facilitate understanding of the physiological function of noggin.
