Alpine-cold regions are characterized as hypoxia,strong wind,heavy rain,cold climate,huge temperature difference between day and night,and vertical climate. All these make it difficult for an emergency rescue when a n...Alpine-cold regions are characterized as hypoxia,strong wind,heavy rain,cold climate,huge temperature difference between day and night,and vertical climate. All these make it difficult for an emergency rescue when a natural disaster such as earthquake happens. Based on the characteristics of emergency rescue in alpinecold region,several multifunctional protective equipments have been developed by the Quartermaster Equipment Institute of General Logistics Departmen(tGLD)of the Chinese People's Liberation Army(CPLA). These equipments are lightweight,durable and environment adaptable.展开更多
Gymnadenia conopsea,an alpine Orchidaceae plant,was one of the widely used Tibetan traditional medicines.In this study,we sequenced total 105 expressed sequence tags (ESTs) from a full-length cDNA expression library c...Gymnadenia conopsea,an alpine Orchidaceae plant,was one of the widely used Tibetan traditional medicines.In this study,we sequenced total 105 expressed sequence tags (ESTs) from a full-length cDNA expression library constructed by the Oligo-capping technique.The further bioinformatic analyses suggested that the 65 represented unique sequences showed high homology to previously identified genes in other plants:30 sequences matched to other uncharacterized expressed sequence tags (ESTs),and 10 sequences showed no good matches to available sequences in DNA databases.Gene ontology annotation by InterProScan indicated that many of these cDNAs (7 percent) have no known molecular functions and may be unique to G.conopsea.Fifty-five ESTs with matched proteins were involved in a series of diverse functions,in which molecular function such as 'binding' (42.9 percent) and 'catalytic activity' (25.0 percent) were the most frequent functions of the cDNAs.This cDNA library provided a critical basis for further investigation of functional genes expression under cold stress in this alpine species.In addition,13 ESTs-based polymerase chain reaction (PCR) primers were designed and can also be used for genotypic identification and for the genetic diversity analysis of G.conopsea and its closely related species.展开更多
文摘Alpine-cold regions are characterized as hypoxia,strong wind,heavy rain,cold climate,huge temperature difference between day and night,and vertical climate. All these make it difficult for an emergency rescue when a natural disaster such as earthquake happens. Based on the characteristics of emergency rescue in alpinecold region,several multifunctional protective equipments have been developed by the Quartermaster Equipment Institute of General Logistics Departmen(tGLD)of the Chinese People's Liberation Army(CPLA). These equipments are lightweight,durable and environment adaptable.
基金the Program for New Century Excellent Talents in University (NCET-06-0810)Young Scholars Foundation from the Science and Technology Committee of Sichuan,China (2008-31-371) for their financial support
文摘Gymnadenia conopsea,an alpine Orchidaceae plant,was one of the widely used Tibetan traditional medicines.In this study,we sequenced total 105 expressed sequence tags (ESTs) from a full-length cDNA expression library constructed by the Oligo-capping technique.The further bioinformatic analyses suggested that the 65 represented unique sequences showed high homology to previously identified genes in other plants:30 sequences matched to other uncharacterized expressed sequence tags (ESTs),and 10 sequences showed no good matches to available sequences in DNA databases.Gene ontology annotation by InterProScan indicated that many of these cDNAs (7 percent) have no known molecular functions and may be unique to G.conopsea.Fifty-five ESTs with matched proteins were involved in a series of diverse functions,in which molecular function such as 'binding' (42.9 percent) and 'catalytic activity' (25.0 percent) were the most frequent functions of the cDNAs.This cDNA library provided a critical basis for further investigation of functional genes expression under cold stress in this alpine species.In addition,13 ESTs-based polymerase chain reaction (PCR) primers were designed and can also be used for genotypic identification and for the genetic diversity analysis of G.conopsea and its closely related species.
