[Objective] To analyze the components and structure of Opuntia milpa alta polysaccharides, and to study its anticoagulant activity. [Method] The crude polysaccharides （CP） were prepared from Opuntia milpa alta using...[Objective] To analyze the components and structure of Opuntia milpa alta polysaccharides, and to study its anticoagulant activity. [Method] The crude polysaccharides （CP） were prepared from Opuntia milpa alta using hot water extraction and ethanol precipitation method, purified by anion-exchange chromatography and gel filtration chromatography to study its monosaccharide composition, weight-average molecular weight distribution and main chain structure. And a preliminary study was conducted on the anticoagulant activity of polysaccharide components. [Result] The polysaccharide components WSP1, WSP2a and WSP3 were obtained from CP by the anion-exchange chromatography and gel filtration chromatography. WSP1 was mainly composed of galactose with a weight-average molecular weight of 2.32×106. WSP2a consisted of rhamnose, arabinose, xylose, mannose, glucose, galactose, glucuronic acid and galacturonic acid, the contents were 6.3%, 32.3%, 12.9%, 1.5%, 4.8%, 37.1%, 0.64% and 4.4%, respectively. WSP3 mainly composed of arabinose, xylose, rhamnose and trace of glucose, mannose, uronic acid. The weight-average molecular weight of WSP2a and WSP3 was 1.24×106 and 7.92×106, respectively. WSP2 prolonged the activated partial thromboplatin time （APTT） and thrombin time （TT）, while had no significant effect on the prothrombin time （PT） in vitro, indicating that anticoagulant activity of WSP2 functions by affecting the intrinsic coagulation system. The test of inhibition to the conversion of fibrinogen into thrombin showed that WSP2a and WSP3 had obvious anticoagulant activity. Methylation analysis indicated that the main chain of WSP2a was constituted of 1, 4-GalA, 1, 2-Rha and 1, 2, 4-Rha. [Conclusion] This study provided experimental basis for the optimization and development of polysaccharide resources from cactus展开更多
[Objective] Genomic in situ hybridization (GISH) was used to study the relationship between the two CCDD genomes of Oryza alta and Oryza latifolia. [Method] Total DNA of Oryza officinalis (C-genome) was used as a prob...[Objective] Genomic in situ hybridization (GISH) was used to study the relationship between the two CCDD genomes of Oryza alta and Oryza latifolia. [Method] Total DNA of Oryza officinalis (C-genome) was used as a probe for genomic in situ hybridization on metaphase chromosomes from Oryza alta and Oryza latifolia, respectively. [Result] Under certain post-hybridization washing stringencies, C- and D-genome could be distinguished in CCDD genome type; there were huge differences in some CC chromosomes of Oryza alta, Oryza latifolia, and Oryza officinalis. The genome of Oryza latifolia was more original. [Conclusion] Comparative analysis of the Oryza species with identical genome type may facilitate to elucidate the possible approaches to plant genome evolution and species evolution.展开更多
In order to reveal the origin and evolutionary relationship between two CCDD genome species, Oryza alta and Oryza latifolia, fluorescence in situ hybridization （FISH） was adopted to analyze the genomes of the two sp...In order to reveal the origin and evolutionary relationship between two CCDD genome species, Oryza alta and Oryza latifolia, fluorescence in situ hybridization （FISH） was adopted to analyze the genomes of the two species with C0t-1 DNA from O. alta as a probe. Karyotype was also comparatively analyzed between O. alta and O. latifolia based on their similar band patterns of the hybridization signals. There were a high homology and close relationship between O. alta and O. latifolia, however, the distinction between the hybridization signals was also clear. C0t-1 DNA was proved to be species- and genome type-specific. It is suggested that C0t-1 DNA-FISH could be more efficient to analyze the genomic relationship between different species. According to the comparative analysis of highly and moderately repetitive DNA sequences between the two allotetraploidy species, O. alta and O. latifolia, the possible origin and evolutionary mechanism of allotetraploidy of Oryza were discussed.展开更多
Opuntia Milpa Alta is a cactus cultivated,domesticated,hybridized and selected from the plant Opuntia ficusindica by Mexican agricultural experts,which can be used as fruit and vegetable.Opuntia Milpa Alta leaves and ...Opuntia Milpa Alta is a cactus cultivated,domesticated,hybridized and selected from the plant Opuntia ficusindica by Mexican agricultural experts,which can be used as fruit and vegetable.Opuntia Milpa Alta leaves and fruit are superior to wild varieties and suitable for storage and transportation.In 1998,Opuntia Milpa Alta was introduced to China from Mexico by the Quality Product Development Center of the Ministry of Agriculture of China.Up to now,the Opuntia Milpa Alta has been cultivated on a certain scale in China.This study aims to identify the research progress and development trends of Opuntia Milpa Alta in China.Papers published between 1998 to 2019 from two major Chinese academic databases(CNKI and Wangfang)with a topic search related to Opuntia Milpa Alta were collected.The research progress and development trends were analyzed based on CiteSpace software of text mining and visualization.The analysis found that Opuntia Milpa Alta has gone through three obvious research phases after being introduced to China.In the first phase,the researchers paid attention to its cultivation method.Subsequently,researchers began to use extraction methods to extract some of its components,such as polysaccharides and flavonoids.Finally,these extracted ingredients began to be used in some biomedical research.展开更多
9月22日,索尼中国专业系统集团在北京AIT索尼影像技术学院隆重举行Cine Alta V电影摄影机发布会,作为索尼Cine Alta摄影机系统的新一代产品,同时也是影视行业的第一款采用全画幅CMOS（36.2×24.1mm）的摄影机,Cine Alta V面世的消息...9月22日,索尼中国专业系统集团在北京AIT索尼影像技术学院隆重举行Cine Alta V电影摄影机发布会,作为索尼Cine Alta摄影机系统的新一代产品,同时也是影视行业的第一款采用全画幅CMOS（36.2×24.1mm）的摄影机,Cine Alta V面世的消息自9月发布以来便引发了影视行业用户持续的关注和讨论。发布会上,索尼专家和产品经理详解其性能优势、研发背景和多项突破性的＂业界第一＂,并与媒体、业界用户以现场问答、网络直播的方式进行互动,力求全面、精确、展开更多
The study reports the habitats of Directive 92/43/EEC in National Park of Alta Murgia （Apulia Region, southem Italy）. The habitats have been identified based on phytosociological analysis. New records are presented ...The study reports the habitats of Directive 92/43/EEC in National Park of Alta Murgia （Apulia Region, southem Italy）. The habitats have been identified based on phytosociological analysis. New records are presented of rocky, meadow, woodlands and temporary ponds habitats, such as： Stipo bromoidis-Quercetum dalechampii （habitat： eastern white oak woods, 91AA＊）; Verbenion supinae （habitat： Mediterranean temporary ponds, 3170＊）; Campanulo versicoloris-Dianthion japigici （habitat： calcareous rocky slopes with chasmophytic vegetation, 8210）; Acino suaveolentis-Stipetum austroitalicae （habitat： eastern sub-mediterranean dry grasslands （Scorzoneretalia villosae）, 62A0）. Other information on their extension, major threat and suggestions of actions for their conservation are provided.展开更多
基金Supported by the National Natural Science Foundation of China(31173753)the International Scientific and Technological Cooperation Project of Anhui Province(10080703035)~~
文摘[Objective] To analyze the components and structure of Opuntia milpa alta polysaccharides, and to study its anticoagulant activity. [Method] The crude polysaccharides （CP） were prepared from Opuntia milpa alta using hot water extraction and ethanol precipitation method, purified by anion-exchange chromatography and gel filtration chromatography to study its monosaccharide composition, weight-average molecular weight distribution and main chain structure. And a preliminary study was conducted on the anticoagulant activity of polysaccharide components. [Result] The polysaccharide components WSP1, WSP2a and WSP3 were obtained from CP by the anion-exchange chromatography and gel filtration chromatography. WSP1 was mainly composed of galactose with a weight-average molecular weight of 2.32×106. WSP2a consisted of rhamnose, arabinose, xylose, mannose, glucose, galactose, glucuronic acid and galacturonic acid, the contents were 6.3%, 32.3%, 12.9%, 1.5%, 4.8%, 37.1%, 0.64% and 4.4%, respectively. WSP3 mainly composed of arabinose, xylose, rhamnose and trace of glucose, mannose, uronic acid. The weight-average molecular weight of WSP2a and WSP3 was 1.24×106 and 7.92×106, respectively. WSP2 prolonged the activated partial thromboplatin time （APTT） and thrombin time （TT）, while had no significant effect on the prothrombin time （PT） in vitro, indicating that anticoagulant activity of WSP2 functions by affecting the intrinsic coagulation system. The test of inhibition to the conversion of fibrinogen into thrombin showed that WSP2a and WSP3 had obvious anticoagulant activity. Methylation analysis indicated that the main chain of WSP2a was constituted of 1, 4-GalA, 1, 2-Rha and 1, 2, 4-Rha. [Conclusion] This study provided experimental basis for the optimization and development of polysaccharide resources from cactus
文摘[Objective] Genomic in situ hybridization (GISH) was used to study the relationship between the two CCDD genomes of Oryza alta and Oryza latifolia. [Method] Total DNA of Oryza officinalis (C-genome) was used as a probe for genomic in situ hybridization on metaphase chromosomes from Oryza alta and Oryza latifolia, respectively. [Result] Under certain post-hybridization washing stringencies, C- and D-genome could be distinguished in CCDD genome type; there were huge differences in some CC chromosomes of Oryza alta, Oryza latifolia, and Oryza officinalis. The genome of Oryza latifolia was more original. [Conclusion] Comparative analysis of the Oryza species with identical genome type may facilitate to elucidate the possible approaches to plant genome evolution and species evolution.
基金supported by the Science Foundation of South-Central University for Nationalities, China (Grant No. YZY10007)
文摘In order to reveal the origin and evolutionary relationship between two CCDD genome species, Oryza alta and Oryza latifolia, fluorescence in situ hybridization （FISH） was adopted to analyze the genomes of the two species with C0t-1 DNA from O. alta as a probe. Karyotype was also comparatively analyzed between O. alta and O. latifolia based on their similar band patterns of the hybridization signals. There were a high homology and close relationship between O. alta and O. latifolia, however, the distinction between the hybridization signals was also clear. C0t-1 DNA was proved to be species- and genome type-specific. It is suggested that C0t-1 DNA-FISH could be more efficient to analyze the genomic relationship between different species. According to the comparative analysis of highly and moderately repetitive DNA sequences between the two allotetraploidy species, O. alta and O. latifolia, the possible origin and evolutionary mechanism of allotetraploidy of Oryza were discussed.
基金supported by the National Natural Science Foundation of China(22004026)Guangdong Provincial Department of Education Young Innovative Talents Project(2019KQNCX179)North Jiangsu Science and Technology Special Project(SZ-SQ2017006).
文摘Opuntia Milpa Alta is a cactus cultivated,domesticated,hybridized and selected from the plant Opuntia ficusindica by Mexican agricultural experts,which can be used as fruit and vegetable.Opuntia Milpa Alta leaves and fruit are superior to wild varieties and suitable for storage and transportation.In 1998,Opuntia Milpa Alta was introduced to China from Mexico by the Quality Product Development Center of the Ministry of Agriculture of China.Up to now,the Opuntia Milpa Alta has been cultivated on a certain scale in China.This study aims to identify the research progress and development trends of Opuntia Milpa Alta in China.Papers published between 1998 to 2019 from two major Chinese academic databases(CNKI and Wangfang)with a topic search related to Opuntia Milpa Alta were collected.The research progress and development trends were analyzed based on CiteSpace software of text mining and visualization.The analysis found that Opuntia Milpa Alta has gone through three obvious research phases after being introduced to China.In the first phase,the researchers paid attention to its cultivation method.Subsequently,researchers began to use extraction methods to extract some of its components,such as polysaccharides and flavonoids.Finally,these extracted ingredients began to be used in some biomedical research.
文摘9月22日,索尼中国专业系统集团在北京AIT索尼影像技术学院隆重举行Cine Alta V电影摄影机发布会,作为索尼Cine Alta摄影机系统的新一代产品,同时也是影视行业的第一款采用全画幅CMOS（36.2×24.1mm）的摄影机,Cine Alta V面世的消息自9月发布以来便引发了影视行业用户持续的关注和讨论。发布会上,索尼专家和产品经理详解其性能优势、研发背景和多项突破性的＂业界第一＂,并与媒体、业界用户以现场问答、网络直播的方式进行互动,力求全面、精确、
文摘The study reports the habitats of Directive 92/43/EEC in National Park of Alta Murgia （Apulia Region, southem Italy）. The habitats have been identified based on phytosociological analysis. New records are presented of rocky, meadow, woodlands and temporary ponds habitats, such as： Stipo bromoidis-Quercetum dalechampii （habitat： eastern white oak woods, 91AA＊）; Verbenion supinae （habitat： Mediterranean temporary ponds, 3170＊）; Campanulo versicoloris-Dianthion japigici （habitat： calcareous rocky slopes with chasmophytic vegetation, 8210）; Acino suaveolentis-Stipetum austroitalicae （habitat： eastern sub-mediterranean dry grasslands （Scorzoneretalia villosae）, 62A0）. Other information on their extension, major threat and suggestions of actions for their conservation are provided.
