SWOT Analysis of Phalaenopsis amabilis Industry in Zhangzhou City

Phalaenopsis amabilis industry in Zhangzhou City is analyzed by SWOT method,and the strengths,weaknesses,opportunities and threats of P.amabilis industry in the city are expounded.Furthermore,some suggestions for the development of P.amabilis industry in Zhangzhou City are put forward,in order to provide certain references for the development of P.amabilis industry.

Two New Glycosides from Rubus amabilis

A pregnane glycoside and a lignan glycoside were isolated from the aerial parts of Rubus amabilis. Their structures were elucidated as 3-O-beta-D-glucopyranosyl-3 beta.15 alpha-dihydroxypregn-5-en-20-one and (-)-secoisolariciresinol-O-alpha-L-rhamnopyranoside using spectroscopic and chemical methods.

Proteomics-Based Analysis of <i>Phalaenopsis amabilis</i>in Response toward <i>Cymbidium</i>Mosaic Virus and/or <i>Odontoglossum</i>Ringspot Virus Infection

Stress response at the protein level to viral infection in orchid plants has not been extensively investigated to date. To understand the proteomic basis of Phalaenopsis amabilis’s responses to Cymbidium Mosaic virus (CymMV), and/or Odontoglossum ring spot virus (ORSV), the total proteins were extracted from Phalaenopsis amabilis leaves infected with CymMV, ORSV, or both respectively. Differentially expressed proteins were identified by two-dimensional electrophoresis, and 27 of these proteins that had significant changes were further examined by mass spectrometry. Comparing CymMV-infected leaves with mock-inoculated ones, 2 proteins were significantly up-regulated, 9 were significantly down-regulated and 1 previously undetected protein was identified. 10 proteins were significantly up-regulated, 3 significantly down-regulated and 1 previously undetected protein was identified in ORSV-infected leaves. 6 proteins were significantly up-regulated and 9 significantly down-regulated proteins were found in co-infected leaves. These identified proteins are involved in disease resistance, stress response, transcriptional regulation, energy metabolism, protein modification and the previously unknown proteins were not involved with known protein pathways. Proteins significantly up-regulated were ATP sulfurylase, down-regulated proteins included glutamate decarboxylase isozyme 2, RNA polymerase alpha subunit and chloroplastic peptide deformylase 1A were proteins with similar alteration trend after all infection treatments. Significantly up-regulated were Thioredoxin H-type and down-regulated Cytosolic phosphoglycerate kinase I which were proteins that have been shown to be specifically regulated by the infection with CymMV. Significantly up-regulated were proteins like Rubisco large subunit, Triosephosphate isomerase, NADP-specific isocitrate dehydrogenase and Cinnamoyl CoA reductase CCR2 by the infection of ORSV. Protein regulation in coinfected leaves followed a pattern similar to that of any of the single virus infection results.

黄山花楸幼苗对遮荫的形态、解剖和光合生理响应

在遮荫强度为0、67%、76%、92%4种处理下,研究1年生黄山花楸(Sorbus amabilis)幼苗对遮荫的形态、解剖和光合生理响应。结果表明:遮荫使幼苗高生长、叶面积增加,地径、主根长减少,造成高径比和比叶面积升高;遮荫改变了幼苗的生物量积累和分配方式,67%和76%的遮荫促进生物量积累,而92%的遮荫则产生抑制作用,遮荫下生物量分配向地上部分倾斜,根生物量比降低,茎、叶生物量比及冠根比升高;随光照强度的减弱,叶片逐渐变薄,栅栏组织与海绵组织比值降低,叶绿素质量分数增加;几种处理的净光合速率日变化均为单峰型,遮荫使日平均净光合速率和蒸腾速率降低。试验表明遮荫诱发了黄山花楸幼苗的形态和光合生理适应机制,适度遮荫可以提升苗木质量。

冀南地区蝴蝶兰新品种引种筛选试验

在冀南地区引进6个蝴蝶兰(Phalaenopsis amabilis)红花系列新品种进行栽培试验,对其开花质量、花期长短、栽培性状、适应性进行观测分析,结合市场认同度对各品种进行综合评价,筛选出四季春、满堂红、大红袍、内山姑娘4个品种为综合评价高、适宜在冀南地区种植推广的优良品种。

蝴蝶兰组培苗快繁高效安全技术

[目的]建立蝴蝶兰组培苗的快繁工厂化生产体系。[方法]以蝴蝶兰的花梗为外植体进行组织培养,并进行继代培养、壮苗培养、生根培养、炼苗、出瓶培养,最后分别包装、标识、运输。[结果]试验获得了无菌的蝴蝶兰芽苗,经8代继代培养后,进行壮苗培养和生根培养,而后进行炼苗与出瓶培养,最后按照大、中、小3个规格的苗龄分级出圃,对出圃的组培苗按级分别包装、标识、运输,形成了一套完整的详细的蝴蝶兰苗快繁工厂化生产体系。[结论]该方法建立了蝴蝶兰组培苗的快繁工厂化生产体系,为蝴蝶兰的进一步开发利用提供了依据。

蝴蝶兰叶斑病病原真菌的分离与鉴定

从蝴蝶兰(Phalaenopsis amabilis)叶片病斑部位分离到5株真菌,通过形态观察及rDNA-ITS序列分析,结果表明,这5株真菌株属于2个分类单元,一个鉴定为腐皮镰刀菌,另一个暂定为Phialo-phora sp.,其致病性有待用柯赫氏法则验证。

Main Anatomy Characteristics in Cell Wall and Lignin Distribution of Bamboo Culms(Pseudosasa amabilis)

The anatomy characteristics of cell wall and lignin distribution in different anatomical regions for 12-month-old bamboo species Pseudosasa amabilis was investigated.The lignin distribution was studied by means of optical microscope and confocal laser scanning microscope and the color reaction. In addition,the distribution of lignin in different tissues(fiber,parenchyma and vessel) was investigated by visible-light microspectrophotometry coupled with the Wiesner and Maule reaction.The cell walls of different tissues were all lignified and the lignin was widely distributed in different tissues, in which there was a difference between tissues and anatomical regions in lignin content.Guaiacyl lignin and syringyl lignin unit could be found in cell wall of fiber,parenchyma and vessel through Wiesner and Maule reaction and the absorbance peak of spectrum. There was no regular variation in lignin content with different radial location of culms and different location in fiber strands.The lamellation of the fiber cell wall was generally had as alternating broad and narrow layers with alternating low and high concentration of lignin.

猬实‘追梦人’嫩枝扦插繁殖技术

以猬实‘追梦人’(Kolkwitzia amabilis‘Dream Catcher’)为材料,研究了插穗部位、扦插基质及生根剂浓度对插穗生根数量及质量的影响。研究结果表明:插穗部位、基质配比和生根剂浓度均显著影响猬实‘追梦人’嫩枝扦插生根效果,在珍珠岩和播种土(2∶1)基质条件下,结合插穗速蘸生根剂处理,顶梢插穗扦插激素浓度2500 mg/L,中下段插穗扦插激素浓度5000 mg/L,在此条件下,猬实‘追梦人’扦插生根率及生根质量最佳。

墨兰和蝴蝶兰镰刀菌病害的鉴定

对广东栽培墨兰(Cymbidium sinense (Andr.) Willd.)和蝴蝶兰(Phalaenopsis amabilis Blume)的镰刀菌病害进行了鉴定.经症状观察、病原菌分离培养与回接、病原菌形态观察和内部转录间隔区(Internal transcribed space,ITS)、延伸因子-1α(Elongation factor-1α,EF-1α)序列分析,鉴定出墨兰茎腐病、蝴蝶兰茎基腐病和蝴蝶兰花梗腐烂病等3种镰刀菌病害,其病原菌分别为尖孢镰刀菌(Fusarium oxysporum)、茄镰刀菌(F.solani)和藤仓镰刀菌(F.Fujikuroi).其中,蝴蝶兰花梗腐烂病(F.fujikuroi)为兰花上首次报道.

蝴蝶兰“大辣椒”花梗组织快繁技术研究

以蝴蝶兰"大辣椒"(Phalaenopsis amabilis Big Chilli)花梗为外植体,研究不同培养基、生长调节物质对蝴蝶兰组培各个阶段产生的影响,建立蝴蝶兰"大辣椒"的离体快繁技术体系。结果表明0.1%的升汞消毒10min效果最佳,污染率最低可达到27.69%,成活率80.75%;腋芽萌发最佳培养基为MS+6-BA 2.0mg/L+NAA 0.05mg/L+蔗糖30g/L+活性炭1g/L+琼脂6g/L,pH值5.6,萌发率82.99%;增殖最佳培养为MS+6-BA 5.0mg/L+NAA 0.1mg/L+蔗糖30g/L+活性炭1g/L+琼脂6g/L pH值5.6,增殖系数2.75,暗培养7d有利于增殖;添加了IBA 2.0mg/L的1/2 MS培养基上,生根最好。