The total content of the rich amino acids in two common red algae, Polysiphonia urceolata and Polysiphonia japonica growing in the Qingdao seashore were determined. The algae powder was hydrolyzed by 6 mol/L HCl at 11...The total content of the rich amino acids in two common red algae, Polysiphonia urceolata and Polysiphonia japonica growing in the Qingdao seashore were determined. The algae powder was hydrolyzed by 6 mol/L HCl at 110℃ for 48 h and determined by amino acid analyzer. The content was 25.35% and 24.16%, respectively, much higher than that of some other species. In addition, a nutritive liquid with abundant amino acids was prepared (by the enzymatic hydrolysis method using Polysiphonia urceolata ) as raw material for a kind of health beverage. The dried seaweed was decolored by 0.25% KMnO 4 and 0.5% active carbon, then enzymalized. In the selection of enzymalizing condition, the orthogonal experimental design was used. Four factors including kinds of enzyme, quantity of enzyme, temperature and time were studied at 3 levels. According to the orthogonal design results, we can choose an optimal condition: hydrolyzing at 45℃ by neutral proteinase (0.25%, w/w) for 2 h, adjusting pH to 8.5, then adding trypsin (0.25%, w/w) and hydrolyzing for 2 h. Finally the above solution was alkalized by NaOH and neutralized by casein. After the hydrolyzed liquid was filtered and concentrated, suitable additives were added. The final products contain rich amino acids.展开更多
A novel efficient method for hydrolyzing feather keratin using an ultrasonic-ionic liquid coupling process has been developed, with reaction conditions optimized using response surface analysis of data obtained from s...A novel efficient method for hydrolyzing feather keratin using an ultrasonic-ionic liquid coupling process has been developed, with reaction conditions optimized using response surface analysis of data obtained from single factor optimization studies. Ultrasonic irradiation(225 W power) of feathers in 8.4 mol·L^(-1) hydrochloric acid containing 1-butyl-3-methylimidazolium chloride as a cosolvent at 80 °C for 1.4 h, followed by heating at 110 °C for 8.3 h, resulted in hydrolysis of their keratin component in an excellent 83.1% yield. Compared with previous methods, this new method employs reduced amounts of hydrochloric acid, shorter reaction time, and affords amino acid hydrolysis products in higher yield.展开更多
文摘The total content of the rich amino acids in two common red algae, Polysiphonia urceolata and Polysiphonia japonica growing in the Qingdao seashore were determined. The algae powder was hydrolyzed by 6 mol/L HCl at 110℃ for 48 h and determined by amino acid analyzer. The content was 25.35% and 24.16%, respectively, much higher than that of some other species. In addition, a nutritive liquid with abundant amino acids was prepared (by the enzymatic hydrolysis method using Polysiphonia urceolata ) as raw material for a kind of health beverage. The dried seaweed was decolored by 0.25% KMnO 4 and 0.5% active carbon, then enzymalized. In the selection of enzymalizing condition, the orthogonal experimental design was used. Four factors including kinds of enzyme, quantity of enzyme, temperature and time were studied at 3 levels. According to the orthogonal design results, we can choose an optimal condition: hydrolyzing at 45℃ by neutral proteinase (0.25%, w/w) for 2 h, adjusting pH to 8.5, then adding trypsin (0.25%, w/w) and hydrolyzing for 2 h. Finally the above solution was alkalized by NaOH and neutralized by casein. After the hydrolyzed liquid was filtered and concentrated, suitable additives were added. The final products contain rich amino acids.
基金Supported by the National Natural Science Foundation of China(21676143)the Jiangsu Synergetic Innovation Center for Advanced Bio-Manufacture,Self-Owned Research Project from Key Laboratory of Material-Oriented Chemical Engineering(ZK201603)Qing Lan Project of Jiang Su Province
文摘A novel efficient method for hydrolyzing feather keratin using an ultrasonic-ionic liquid coupling process has been developed, with reaction conditions optimized using response surface analysis of data obtained from single factor optimization studies. Ultrasonic irradiation(225 W power) of feathers in 8.4 mol·L^(-1) hydrochloric acid containing 1-butyl-3-methylimidazolium chloride as a cosolvent at 80 °C for 1.4 h, followed by heating at 110 °C for 8.3 h, resulted in hydrolysis of their keratin component in an excellent 83.1% yield. Compared with previous methods, this new method employs reduced amounts of hydrochloric acid, shorter reaction time, and affords amino acid hydrolysis products in higher yield.
