Object:To study the effect of the amniotic membrane on enhancing the proliferation of corneal epithelia and YAC-1 cell.Methods:After the primary culture of the rabbit's corneal epithelia and YAC-1 cells.they were...Object:To study the effect of the amniotic membrane on enhancing the proliferation of corneal epithelia and YAC-1 cell.Methods:After the primary culture of the rabbit's corneal epithelia and YAC-1 cells.they were seeded on the upper surface or stromal matrix side of amniotic membrane respectively.The proliferation results were observed by MTT test.Results:The amniotic membrane was found singificantly enhancing the proliferation of corneal epithelia on the d1,d3 ,and 45 after culture.The proliferation rate was 28.93%,23.32%,23.41%(P<0.05) respectively,but the d7 proliferation rate was 20.72%(P>0.05).On the d1,d3,d7 after culture,the YAC-1 cells proliferation rate was 34.87%,36.28%,33.86%（P<0.01) respectively.Conclusion :Our results demonstrated that the amniotic membrane could enhance the proliferation of both corneal epithelia and YAC-1 cells significantly.Although amniotic membrane has been suggested as an ideal material for reconstruction of ocular surface,spicial attention should be paid during amniotic membrane transplantation for treating ocular surface lesion resulted from epibulbar tumors.展开更多
文摘Object:To study the effect of the amniotic membrane on enhancing the proliferation of corneal epithelia and YAC-1 cell.Methods:After the primary culture of the rabbit's corneal epithelia and YAC-1 cells.they were seeded on the upper surface or stromal matrix side of amniotic membrane respectively.The proliferation results were observed by MTT test.Results:The amniotic membrane was found singificantly enhancing the proliferation of corneal epithelia on the d1,d3 ,and 45 after culture.The proliferation rate was 28.93%,23.32%,23.41%(P<0.05) respectively,but the d7 proliferation rate was 20.72%(P>0.05).On the d1,d3,d7 after culture,the YAC-1 cells proliferation rate was 34.87%,36.28%,33.86%（P<0.01) respectively.Conclusion :Our results demonstrated that the amniotic membrane could enhance the proliferation of both corneal epithelia and YAC-1 cells significantly.Although amniotic membrane has been suggested as an ideal material for reconstruction of ocular surface,spicial attention should be paid during amniotic membrane transplantation for treating ocular surface lesion resulted from epibulbar tumors.
