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An efficient synthesis of ampicillin on magnetically separable immobilized penicillin G acylase 被引量:6
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作者 Ping Xue Xiao Dan Song Xue Rong Cao 《Chinese Chemical Letters》 SCIE CAS CSCD 2010年第7期765-768,共4页
Penicillin G acylase(PGA) was immobilized on the magnetic hydrophilic polymer microspheres with average pore size of 17.1 nm,specific surface area of 128.2 m2/g and saturate magnetization of 6.4 emu/g.The 96.7%ampicil... Penicillin G acylase(PGA) was immobilized on the magnetic hydrophilic polymer microspheres with average pore size of 17.1 nm,specific surface area of 128.2 m2/g and saturate magnetization of 6.4 emu/g.The 96.7%ampicillin yield with 1.60 of the synthesis/hydrolysis(S/H) ratio from 6-aminopenicillanic acid(6-APA) and D-(-)-alpha-phenylglycine methyl ester(D-PGME) can be achieved using the resultant magnetic biocatalyst in ethylene glycol,where only 82.1%yield with 1.40 of the S/H ratio was obtained using the free PGA under the identical reaction conditions.The immobilized PGA can be separated magnetically and recycled for five times without obvious loss of its catalytic activity. 展开更多
关键词 Immobilized penicillin G acylase Magnetic polymer microspheres ampicillin synthesis REUSABILITY
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A novel surface molecularly imprinted polymer as the solid-phase extraction adsorbent for the selective determination of ampicillin sodium in milk and blood samples 被引量:6
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作者 Ningli Wu Zhimin Luo +7 位作者 Yanhui Ge Pengqi Guo Kangli Du Weili Tang Wei Du Aiguo Zeng Chun Chang Qiang Fu 《Journal of Pharmaceutical Analysis》 SCIE CAS 2016年第3期157-164,共8页
Surface molecularly imprinted polymers (SMIPs) for selective adsorption of ampicillin sodium were synthesized using surface molecular imprinting technique with silica gel as a support. The physical and morphological... Surface molecularly imprinted polymers (SMIPs) for selective adsorption of ampicillin sodium were synthesized using surface molecular imprinting technique with silica gel as a support. The physical and morphological characteristics of the polymers were investigated by scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FT-IR), thermogravimetric analysis (TGA), elemental analysis and nitrogen adsorption-desorption test. The obtained results showed that the SMIPs displayed great adsorption capacity (13.5 lag/mg), high recognition ability (the imprinted factor is 3.2) and good binding kinetics for ampicillin sodium. Finally, as solid phase extraction adsorbents, the SMIPs coupled with HPLC method were validated and applied for the enrichment, purification and determination of anapicillin sodium in real milk and blood samples. The averages of spiked accuracy ranged from 92.1% to 107.6%. The relative standard deviations of intra- and inter-day precisions were less than 4.6%. This study provides a new and promising method for enriching, extracting and determining ampicillin sodium in complex biological samples. 展开更多
关键词 ampicillin sodium Surface molecularly imprinted polymers Solid phase extraction High performance liquid chromatography
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Magnetic metal organic framework for pre-concentration of ampicillin from cow milk samples 被引量:6
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作者 Ahmad Reza Bagheri Mehrorang Ghaedi 《Journal of Pharmaceutical Analysis》 SCIE CAS CSCD 2020年第4期365-375,共11页
The aim of this study is a present of a simple solvothermal synthesis approach to preparation of Cu-based magnetic metal organic framework(MMOF)and subsequently its application as sorbent for ultrasound assisted magne... The aim of this study is a present of a simple solvothermal synthesis approach to preparation of Cu-based magnetic metal organic framework(MMOF)and subsequently its application as sorbent for ultrasound assisted magnetic solid phase extraction(UAMSPE)of ampicillin(AMP)from cow milk samples prior to high performance liquid chromatography-Ultraviolet(HPLC-UV)determination.Characteristics of prepared MMOF were fully investigated by different techniques which showed the exclusive properties of proposed sorbent in terms of proper functionality,desirable magnetic property and also high specific surface area.Different influential factors on extraction recovery including sorbent dosage,ultrasonic time,washing solvent volume and eluent solvent volume were assessed using central composite design(CCD)based response surface methodology(RSM)as an operative and powerful optimization tool.This is the first report for determination of AMP using MMOF.The proposed method addressed some drawbacks of other methods and sorbents for determination of AMP.The presented method decreases the extraction time(4 min)and also enhances adsorption capacity(250 mg/g).Moreover,the magnetic property of presented sorbent(15 emu/g)accelerates the extraction process which does not need filtration,centrifuge and precipitation procedures.Under the optimized conditions,the proposed method is applicable for linear range of 1.0-5000.0 μg/L with detection limit of 0.29 μg/L,satisfactory recoveries(≥95.0%)and acceptable repeatability(RSD less than 4.0%).The present study indicates highly promising perspectives of MMOF for highly effective analysis of AMP in complicated matrices. 展开更多
关键词 Magnetic metal organic framework Ultrasound assisted magnetic solid phase EXTRACTION ampicillin Cow milk samples
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Stability Test of Ampicillin Sodium Solutions in the Accufuser^(■) Elastomeric Infusion Device Using HPLC-UV Method 被引量:2
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作者 Min-A.Kang Ju-Seop Kang 《Pharmacology & Pharmacy》 2012年第4期462-467,共6页
The stabilities of two kinds of solutions (30 mg/mL) of Ampicillin sodium in 0.9% NaCl in water (NS, normal saline) and in sterile water (SW) in the intravenous elastomeric infusion device (Accufuser&#174) were ev... The stabilities of two kinds of solutions (30 mg/mL) of Ampicillin sodium in 0.9% NaCl in water (NS, normal saline) and in sterile water (SW) in the intravenous elastomeric infusion device (Accufuser&#174) were evaluated based on recommended solutions and storage periods. The injectable NS- and SW-Ampicillin solutions in the Accufuser&#174 device were stored and evaluated at controlled temperature (room temperature, 25℃ ± 2℃ and cold temperature, 4℃ ± 2℃) during 7 days. Effects of the periods of storage (from 0 to 7 days) and the temperatures of storage (RT and CT) on the physico-chemical appearances and concentrations of active compounds were determined. The visual clarity, pH, and concentrations of Ampicillin were determined by stability-indicating high-performance liquid chromatography (HPLC)-ultraviolet (UV) detection. The results showed that the amount of Ampicillin in studied solutions gradually decreased with time. The Ampicillin in NS, which was stored in CT, was relatively stable, retaining 94% of its original amount up to 7 days. The solution that showed least stability was Ampicillin in SW, which was stored in RT, retaining 80% of its original amount. Generally, solutions that were stored in CT were more stable than the solutions that were stored in RT. No significant changes in physical appearance or color of the solutions were observed during the study. Particles were not detected in any solution samples. In summary, two kinds of solutions of Ampicillin sodium, in NS and SW, showed different chemical stabilities with time in intravenous infusion device without any significant physical changes and retained about 94% vs 89% and 83% vs 80% of initial concentrations after 7 days in CT and RT, respectively. We suggest that 30 mg/mL of Ampicillin sodium in NS solution in an Accufuser&#174 infusion device which is stored in CT can be applicable for 7 days in clinical situations. 展开更多
关键词 ampicillin Sodium Intravenous Elastomeric Infusion Device(Accufuser^(■)) HPLC-UV Method Solution Stability
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Inhibition of Ampicillin-resistance in Bacteria by Modified DNAzymes
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作者 CHEN Fei LI Zhe +6 位作者 YANG Shuo WANG Rui-jian LIU Bin SONG Yu-ming SUN Yan-hong HAO Dong-yun WANG Xiao-ping 《Chemical Research in Chinese Universities》 SCIE CAS CSCD 2008年第4期491-495,共5页
To overcome ampicillin-resistance of bacteria which is believed to attribute their endogenous B-lactamase, we designed three 10-23 DNAzymes(Dz1, Dz2. Dz3) targeting the coding region of B-lactamase mRNA and examined... To overcome ampicillin-resistance of bacteria which is believed to attribute their endogenous B-lactamase, we designed three 10-23 DNAzymes(Dz1, Dz2. Dz3) targeting the coding region of B-lactamase mRNA and examined their inhibitory capabilities of the ampicillin-resistance of TEM-1 and TEM-3 bacteria. Dz1 was a traditional 10-23 DNAzyme, Dz2 was the mutant of Dz1 by addition of the protected nucleotide to each ann of the enzyme, and Dz3 was a mutant of Dz1 at antisense arms of which phosphorothioate modifications were made. Kinetic analysis, bacterial growth, and β-lactamase activity measurement showed that all the three DNAzymes worked efficiently in vitro and in vivo. A 9 hours bacterial growth inhibition test showed that the inhibition rates of TEM-1 bacteria by Dz1, Dz2, and Dz3 were 27%, 50%, and 29%, respectively. In addition, the inhibition rates of TEM-3 bacteria by those three DNAzymes were found io be 49%, 58%, and 45%, respectively. The current findings suggest that DNAzymes may become potential candidates of alternative inhibitors for bacteria drug-resistance. 展开更多
关键词 DNAZYME DRUG-RESISTANCE Β-LACTAMASE ampicillin Antisense drug
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Ampicillin与Unasyn血清杀菌活性的比较
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作者 王睿 刘又宁 《解放军医学情报》 北大核心 1989年第4期195-197,共3页
关键词 ampicillin UNASYN 杀菌 青霉素
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Sequential Injection Analysis of Ampicillin in Pharmaceuticals by Using Potentiometric Detectors Based on PVC and Sol-Gel Membranes
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作者 Nestor Zarate Alberto N.Araujo +1 位作者 M.Conceicao B.S.M.Montenegro Ricardo Perez-Olmos 《American Journal of Analytical Chemistry》 2011年第4期491-499,共9页
This work describes the construction and the evaluation of the general performance of new ampicilli-nate-selective electrodes based on manganese (III) tetraphenylporphyrin [Mn(III) TPP-Cl] as ionophore, incorporated i... This work describes the construction and the evaluation of the general performance of new ampicilli-nate-selective electrodes based on manganese (III) tetraphenylporphyrin [Mn(III) TPP-Cl] as ionophore, incorporated in both PVC and sol-gel membranes and directly applied onto a conductive graphite/epoxy resin support. The units were constructed without inner reference solution adopting conventional configuration and in the case of PVC membrane the tubular configuration was also adopted. The good working characteristics of these electrodes, made possible its application to the determination of ampicillin in pharmaceuticals formulations, both in conventional batch analysis and in flow conditions, when the electrodes were coupled to a SIA system. In the last case the potentiometric sensors presented linear response towards ampicillin concentration between 5.0 × 10–4 and 5.0 × 10–2 mol?l–1 with slopes of –57.4 and –63.5 mV?dec–1 for the PVC and sol-gel membranes, respectively. The developed procedures enable mean relative standard deviations better than 3% for all the samples analysed. The obtained results do not statistically differ from those furnished by applying the HPLC reference method. 展开更多
关键词 ampicillin SIA Potentiometric Detection Pharmaceutical Formulations
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A laser light scattering study of the interaction between human serum albumin and ampicillin sodium 被引量:3
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作者 WANG Weiping TANG Jianghong PENG Xuhong HU Zhide CHEN Xingguo 《Science China Chemistry》 SCIE EI CAS 2006年第4期332-337,共6页
The complexes formed by the interaction of human serum albumin and ampicillin sodium in aqueous solutions were investigated at 25 ± 0.1℃, ionic strength I = 0.085 mol·kg-1, pH 4.9, 5.8 and 7.4. The results ... The complexes formed by the interaction of human serum albumin and ampicillin sodium in aqueous solutions were investigated at 25 ± 0.1℃, ionic strength I = 0.085 mol·kg-1, pH 4.9, 5.8 and 7.4. The results of static light scattering have suggested that at pH 7.4, 5.8, 4.9, the molecular weight of the protein/drug complexes is 210,000 g·mol-1, 418,000 g·mol-1, 448,000 g·mol-1, re- spectively. The z-average root-mean-square radius of gyration and the second virial coefficients de- crease with pH decreasing. Dynamic light scattering provides information on diffusion coefficient and particle distributions of protein/drug complexes under different conditions, which suggests a broad hydrodynamic diameter range of scatters. The diffusion coefficients of the systems change with am- picillin sodium concentration and pH changing. 展开更多
关键词 human serum ALBUMIN (HSA) ampicillin sodium (AMP) dynamic LIGHT SCATTERING (DLS) static LIGHT SCATTERING (SLS).
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超高效液相色谱-串联质谱法测定鸡蛋中氨苄西林及其代谢物残留
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作者 黎乃添 雍燕 +3 位作者 叶渭源 黎健业 吴玉枝 方炳虎 《中国兽药杂志》 2024年第5期34-44,共11页
建立一种同时测定鸡蛋中氨苄西林及其主要代谢物氨苄西林酸和氨苄西林二酮哌嗪的超高效液相色谱串联质谱方法。样品经乙腈水溶液(70∶30,V/V)提取、浓缩、SAX固相萃取小柱净化后,采用超高效液相色谱串联质谱法进行检测。结果显示,氨苄... 建立一种同时测定鸡蛋中氨苄西林及其主要代谢物氨苄西林酸和氨苄西林二酮哌嗪的超高效液相色谱串联质谱方法。样品经乙腈水溶液(70∶30,V/V)提取、浓缩、SAX固相萃取小柱净化后,采用超高效液相色谱串联质谱法进行检测。结果显示,氨苄西林、氨苄西林酸和氨苄西林二酮哌嗪在1~200 ng/g的浓度范围内呈良好的线性关系。在鸡蛋中氨苄西林、氨苄西林酸和氨苄西林二酮哌嗪的检测限均为0.5 ng/g,定量限为1 ng/g。在1~100 ng/g添加浓度范围内,氨苄西林、氨苄西林酸和氨苄西林二酮哌嗪在鸡蛋中的平均回收率分别为84.0%~96.2%、85.6%~96.4%和89.2%~95.8%,批内、批间RSD均小于10%。添加不同浓度药物的鸡蛋样品具有良好的长期冷冻放置稳定性和反复冻融稳定性。蛋鸡在服用氨苄西林后,所产鸡蛋均能检出氨苄西林、氨苄西林酸和氨苄西林二酮哌嗪,且氨苄西林酸和氨苄西林二酮哌嗪的残留量和残留时间高于氨苄西林原型。上述内容表明,该方法各项技术指标均能满足残留检测要求,可用于定量测定鸡蛋中氨苄西林及其主要代谢物的残留量。 展开更多
关键词 鸡蛋 氨苄西林 超高效液相色谱串联质谱法 药物残留
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Effect of ultraviolet irradiation and chlorination on ampicillin-resistant Escherichia coli and its ampicillin resistance gene 被引量:4
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作者 Yuchen PANG Jingjing HUANG +2 位作者 Jinying XI Hongying HU Yun ZHU 《Frontiers of Environmental Science & Engineering》 CSCD 2016年第3期522-530,共9页
Antibiotic resistance is a serious public health risk that may spread via potable and reclaimed water. Effective disinfection is important for inactivation of antibiotic-resistant bacteria and disruption of antibiotic... Antibiotic resistance is a serious public health risk that may spread via potable and reclaimed water. Effective disinfection is important for inactivation of antibiotic-resistant bacteria and disruption of antibiotic resistance genes. Ampicillin is a widely prescribed antibiotic but its effectiveness is increasingly undermined by resistance. In this study, changes in ampicillin resistance for Escherichia coli (E. coli) CGMCC 1.1595 were analyzed after exposure to different doses of ultraviolet (UV) or chlorine, and damage incurred by the plasmid encoding ampicillin resistance gene blaTEM-1 was assessed. We reported a greater stability in ampicillinresistant E. coli CGMCC 1.1595 after UV irradiation or chlorination when compared with previously published data for other E. coli strains. UV irradiation and chlorination led to a shift in the mortality frequency distributions of ampicillin-resistant E. coli when subse-quently exposed to ampicillin. The ampicillin hemiinhibitory concentration (IC5o) without disinfection was 3800mg·L^-1, and an increment was observed after UV irradiation or chlorination. The IC50 of ampicillin-resistant E. coli was 1.5-fold higher at a UV dose of 40 mJ·cm^-2, and was 1.4-fold higher when exposed to 2.0 mg·L^-1 chlorine. These results indicate that UV irradiation and chlorination can potentially increase the risk of selection for E. coli strains with high ampicillin resistance. There was no evident damage to blaTEM-1 after 1-10 mg Cl2· L^-1 chlorination, while a UV dose of 80 mJ·cm^-2 yielded a damage ratio for blaTEM-1 of approximately 1.2-log.Therefore, high UV doses are required for effective disruption of antibiotic resistance genes in bacteria. 展开更多
关键词 antibiotic resistance Escherichia coli ampicillin resistance gene ultraviolet irradiation CHLORINATION
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abrication of CDs hybrid MIL-68(In) derived In2O_(3)eIn_(2)S_(3) hollow tubular heterojunction and their exceptional self-powered PEC aptasensing properties for ampicillin detecting 被引量:3
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作者 Tao Yan Yixuan Feng +6 位作者 Xiang Ren Jinkai Li Yizhong Lu Meng Sun Liangguo Yan Qin Wei Huangxian Ju 《Journal of Materiomics》 SCIE EI 2021年第4期721-727,共7页
A visible-light-driven self-powered photoelectrochemical(PEC)aptasensor was developed for ampicillin(AMP)detecting based on carbon dots(CDs)hybrid MIL-68(In)-derived In2O_(3)eIn_(2)S_(3) hollow tubular heterojunction(... A visible-light-driven self-powered photoelectrochemical(PEC)aptasensor was developed for ampicillin(AMP)detecting based on carbon dots(CDs)hybrid MIL-68(In)-derived In2O_(3)eIn_(2)S_(3) hollow tubular heterojunction(CDs/In2O_(3)eIn_(2)S_(3)).In_(2)S_(3) nanosheets uniformly grew in-situ on the surface of In2O_(3) hollow tubes,forming a close contact heterogeneous interface,which significantly promoted the transfer and separation of photo-generated carriers,and provided a large specific surface area and rich active sites for the PEC aptasensing platform.Furthermore,the CDs/In2O_(3)eIn_(2)S_(3)/ITO electrode,which was obtained by dipping assembly,showed remarkable and stable photoelectric signals at zero-bias under visible light irradiation.The amino-functionalized AMP aptamer was fixed on the working electrode as a biological recognition element,and the concentration of AMP was determined by observing the change in photocurrent intensity caused by the specific capture of AMP molecules in solution.Under optimized conditions,the developed PEC aptasensor displayed a relatively wide linear range from 0.001 ng mL^(-1) to 300 ng mL^(-1),as well as a low detection limit(LOD)of 0.06 pg mL^(-1).Besides,the novel self-powered PEC AMP-aptasensor exhibited excellent reproducibility,good stability and selectivity,which open a potential avenue for antibiotic residues detection in environmental media. 展开更多
关键词 SELF-POWERED Photoelectrochemical aptasensor MIL-68(In)-derived Hollow tubes ampicillin
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微生物管碟法测定血浆氨苄西林浓度分析
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作者 陶梦筱 刘容序 +3 位作者 阮入琳 熊智慧 周康 吴海港 《现代畜牧科技》 2024年第11期45-49,共5页
该试验以藤黄微球菌为工作菌,按藤黄微球菌细菌生长曲线、菌悬液制备、氨苄西林钠溶液配制、双层培养基的制备进行试验,对精密度、线性关系和回收率试验结果进行讨论,探索了确定血浆中氨苄西林钠浓度的微生物学方法。结果显示,氨苄西林... 该试验以藤黄微球菌为工作菌,按藤黄微球菌细菌生长曲线、菌悬液制备、氨苄西林钠溶液配制、双层培养基的制备进行试验,对精密度、线性关系和回收率试验结果进行讨论,探索了确定血浆中氨苄西林钠浓度的微生物学方法。结果显示,氨苄西林钠浓度在0.04~5μg/mL范围内,药物浓度对数与抑菌圈直径之间有良好线性关系r=0.997,血浆中最低检测线0.04μg/mL。氨苄西林钠浓度在5.0、0.31、0.04μg/mL时,日内变异系数分别时6.55%、7.96%、7.06%;日间变异系数分别是5.97%、6.91%、7.71%。回收率为97.8%~100%。与测定血浆中氨苄西林钠浓度的仪器方法相比,微生物管碟法可靠、准确、灵敏,检定菌易培养,易观察结果,费用低廉,可以满足对血浆中氨苄西林钠浓度分析要求。 展开更多
关键词 氨苄西林 管碟法 血浆 回收率 生长曲线 含量测定
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氨苄西林胶囊质量分析
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作者 李一兰 王丹 +3 位作者 付晓丽 刘旭春 张玲变 周晓溪 《中国抗生素杂志》 CAS CSCD 北大核心 2024年第3期310-316,共7页
目的评价目前国产氨苄西林胶囊的质量现状及存在问题。方法按国家药品抽检计划总体要求,采用法定检验方法结合探索性研究对氨苄西林胶囊样品进行检验,通过对杂质谱、溶出度、溶出曲线、原料药对制剂质量的影响和包装材料对制剂质量的影... 目的评价目前国产氨苄西林胶囊的质量现状及存在问题。方法按国家药品抽检计划总体要求,采用法定检验方法结合探索性研究对氨苄西林胶囊样品进行检验,通过对杂质谱、溶出度、溶出曲线、原料药对制剂质量的影响和包装材料对制剂质量的影响等项目的考察,分析本品的质量状况。结果按法定标准检验103批次样品,合格率100%,与2016年氨苄西林胶囊国抽结果比较,产品质量总体持续稳定。探索性研究对各杂质的来源与结构进行了确证;溶出度测定方法采用HPLC法检测结果与标准检验排除胶囊壳干扰后结果无明显差异;不同企业样品的溶出曲线存在较大差异;原料药的质量影响制剂质量;不同包装氨苄西林胶囊在相同的放置条件下杂质变化差异较大。结论氨苄西林胶囊质量总体较好,现行质量标准需进一步完善,建议修订溶出度测定方法为HPLC法以消除胶囊壳的干扰;建议企业开展仿制药质量和疗效一致性评价,选用质量更好的原料药,选用防潮效果更好的铝塑铝包装,或者在铝塑泡罩外加防潮袋。 展开更多
关键词 氨苄西林 质量分析 溶出曲线 胶囊
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注射用氨苄西林钠质量评价
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作者 刘旭春 姚羽 +3 位作者 付晓丽 王丹 张玲变 周晓溪 《中国抗生素杂志》 CAS CSCD 北大核心 2024年第3期304-309,共6页
目的对注射用氨苄西林钠的质量进行评价。方法采用法定标准结合探索性研究对95批次注射用氨苄西林钠进行检验,并与往年的两次国抽结果进行纵向比较,评价本品的质量状况。结果按法定标准检验,95批次样品全部符合规定。原料为冷冻干燥和... 目的对注射用氨苄西林钠的质量进行评价。方法采用法定标准结合探索性研究对95批次注射用氨苄西林钠进行检验,并与往年的两次国抽结果进行纵向比较,评价本品的质量状况。结果按法定标准检验,95批次样品全部符合规定。原料为冷冻干燥和溶媒结晶工艺的产品在碱度、有关物质、水分、含量等方面存在明显差异。表现为冷冻干燥工艺的产品碱度相对较低,含量(按无水物计算)相对较低,杂质量、水分相对较高。探索性研究表明,在加速试验条件下放置,随着时间的增长,原料为冷冻干燥工艺的产品颜色加深,杂质增大;而溶媒结晶工艺的产品颜色和杂质变化较小。结论注射用氨苄西林钠总体质量较好,采用溶媒结晶工艺为原料的产品质量相对更优,原料是影响本品质量的主要因素。 展开更多
关键词 注射用氨苄西林钠 质量评价 生产工艺
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复合乳酸菌对氨苄西林诱导小鼠腹泻缓解作用
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作者 赵聪瑞 史晓丹 +1 位作者 吕文晴 李柏良 《食品研究与开发》 CAS 2024年第19期13-20,共8页
肠道菌群是人体的一个复杂生态系统,与人体健康密切相关,在多个方面发挥重要作用。抗生素滥用不仅会抑制致病菌,还可能破坏正常的益生菌群。为解决抗生素对肠道菌群的不良影响,该文用低、中、高剂量(1×10^(6)、1×10^(7)、1... 肠道菌群是人体的一个复杂生态系统,与人体健康密切相关,在多个方面发挥重要作用。抗生素滥用不仅会抑制致病菌,还可能破坏正常的益生菌群。为解决抗生素对肠道菌群的不良影响,该文用低、中、高剂量(1×10^(6)、1×10^(7)、1×10^(8) CFU/只)复合乳酸菌(嗜酸乳杆菌LA⁃06、动物双歧杆菌乳亚种XLTG11、干酪乳酪杆菌Glory LC18和鼠李糖乳酪杆菌Glory LG12)处理抗生素干扰的小鼠模型,并测量小鼠体质量、病理变化、肠道屏障、肠道通透性、短链脂肪酸浓度。研究发现复合乳酸菌可以有效地提升小鼠体质量,促进有益菌群生长,维持肠道组织完整性,增强肠道屏障功能,降低肠道屏障通透性。结果表明,复合乳酸菌有极好的益生功能,能够显著改善抗生素导致的小鼠腹泻,且呈剂量依赖性。 展开更多
关键词 肠道屏障 肠道菌群 复合乳酸菌 腹泻 氨苄西林
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柑橘中氨苄青霉素QuEChERS-液质联用检测方法的建立及残留降解动态测定
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作者 唐昭领 莫秋云 +4 位作者 李惠静 韦福广 罗晓慧 陈蔚 陶剑恒 《食品与发酵科技》 CAS 2024年第2期123-128,133,共7页
建立基于石墨化多壁碳纳米管(G-MWCNTs)的改良QuEChERS法结合液质联用技术测定柑橘中氨苄青霉素(Ampicillin)的简便、快速、准确的检测方法。在优选的色谱及质谱条件下,首先考察不同提取溶剂对氨苄青霉素提取回收率的影响;然后考察4种... 建立基于石墨化多壁碳纳米管(G-MWCNTs)的改良QuEChERS法结合液质联用技术测定柑橘中氨苄青霉素(Ampicillin)的简便、快速、准确的检测方法。在优选的色谱及质谱条件下,首先考察不同提取溶剂对氨苄青霉素提取回收率的影响;然后考察4种吸附剂的用量对氨苄青霉素回收率的影响,并优化净化剂使用量。结果表明,氨苄青霉素在0.5~100ng/mL浓度范围内线性关系良好,相关系数(R^(2))均大于0.999,基质效应(matrix effect,MEs)在9.0%~21.2%之间,低、中、高(2、40、200μg/kg)3水平下的平均回收率为85.9%~102.4%,相对标准偏差(Relative Standard Deviation,RSD)为2.1%~5.8%,检测限(Limit of Detection,LOD)和定量限(Limit of Quantity,LOQ)分别为1.0μg/kg和4.0μg/kg。在降解动态试验中,氨苄青霉素在沃柑和脐橙中的降解半衰期分别为1.2d和1.1d。该方法简便、快速、灵敏、准确,可为柑橘的质量评价提供有效的检测方法。 展开更多
关键词 柑橘 氨苄青霉素 液质联用 QUECHERS 降解动态
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超高效液相色谱串联质谱法检测人促红素注射剂中2种抗生素残留
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作者 唐娜 苏超男 +3 位作者 陈统华 肖一桐 王嫦鹤 徐长根 《中国药业》 CAS 2024年第16期86-89,共4页
目的建立检测人促红素注射剂中氨苄西林和博来霉素残留的超高效液相色谱串联质谱(UPLC-MS/MS)法。方法色谱柱为Waters Cortecs T3柱(100 mm×2.1 mm,2.7μm);流动相为0.1%甲酸水溶液-[乙腈-甲醇(95∶5,V/V)],梯度洗脱;流速为0.2 mL/... 目的建立检测人促红素注射剂中氨苄西林和博来霉素残留的超高效液相色谱串联质谱(UPLC-MS/MS)法。方法色谱柱为Waters Cortecs T3柱(100 mm×2.1 mm,2.7μm);流动相为0.1%甲酸水溶液-[乙腈-甲醇(95∶5,V/V)],梯度洗脱;流速为0.2 mL/min;柱温为40℃;进样量为5μL。采用电喷雾离子源正离子模式和多反应监测扫描模式。结果氨苄西林和博来霉素质量浓度分别在19.38~484.5 ng/mL(r=0.9979,n=5)和21.26~212.6 ng/mL(r=0.9988,n=5)范围内与峰面积线性关系良好,检测限分别为0.00969,10.63 ng/mL,定量限分别为0.0969,21.26 ng/mL;精密度、稳定性试验结果的RSD均小于4.0%;平均加样回收率分别为102.11%和101.77%,RSD为分别为7.46%和13.35%;基质效应均不明显(分别为95.03%和84.51%)。9家国内生产企业和1家国外生产企业的39批人促红素注射剂均未检出氨苄西林和博来霉素。结论所建立的方法操作简单、检测灵敏,结果准确,可用于人促红素注射剂中氨苄西林和博来霉素残留的检测。 展开更多
关键词 人促红素注射液 注射用人促红素 超高效液相色谱串联质谱法 氨苄西林 博来霉素 残留 含量测定
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氨苄西林联合羟甲烟胺对慢性胆囊炎患者胆囊功能、氧化应激的影响
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作者 袁昊 《中国实用医药》 2024年第11期117-120,共4页
目的 研究氨苄西林联合羟甲烟胺治疗慢性胆囊炎的临床疗效。方法 100例慢性胆囊炎患者,按随机数表法分为对照组与研究组,每组50例。对照组采用氨苄西林治疗,研究组采用氨苄西林联合羟甲烟胺治疗。比较两组临床疗效、症状(腹胀、恶心、... 目的 研究氨苄西林联合羟甲烟胺治疗慢性胆囊炎的临床疗效。方法 100例慢性胆囊炎患者,按随机数表法分为对照组与研究组,每组50例。对照组采用氨苄西林治疗,研究组采用氨苄西林联合羟甲烟胺治疗。比较两组临床疗效、症状(腹胀、恶心、右上腹疼痛)缓解时间、不良反应发生率及治疗前后胆囊功能(胆囊容积、胆囊壁厚度、胆囊收缩率)、氧化应激指标[丙二醇(MDA)、谷胱甘肽过氧化物酶(GSH-Px)及超氧化物歧化酶(SOD)]。结果 研究组总有效率相比于对照组更高(96.00%VS 78.00%)(P<0.05)。研究组腹胀、恶心、右上腹疼痛缓解时间分别为(4.48±0.81)、(3.84±0.75)、(5.81±0.62)d,均短于对照组的(5.66±0.73)、(4.89±0.82)、(6.69±0.58)d(P<0.05)。治疗1个月后,两组胆囊容积、胆囊壁厚度均下降,胆囊收缩率上升,且研究组胆囊容积(22.29±2.06)cm3、胆囊壁厚度(2.21±0.54)mm低于对照组的(26.49±1.93)cm3、(3.01±0.57)mm,胆囊收缩率(56.82±3.48)%高于对照组的(46.67±2.85)%(P<0.05)。治疗1个月后,两组MDA较治疗前下降,SOD、GSH-Px较治疗前上升,且研究组MDA(9.75±1.53)nmol/ml低于对照组的(14.69±1.69)nmol/ml,SOD(119.65±6.89)U/ml、GSH-Px(78.75±5.62)U/L高于对照组的(106.53±6.78)U/ml、(66.52±4.82)U/L(P<0.05)。研究组不良反应发生率(8.00%)与对照组(4.00%)相比,无显著差异(P>0.05)。结论 氨苄西林联合羟甲烟胺治疗慢性胆囊炎疗效确切,可有效改善患者胆囊功能,减轻氧化应激,且未明显增加不良反应。 展开更多
关键词 慢性胆囊炎 羟甲烟胺 氨苄西林 胆囊功能 氧化应激
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无水氨苄西林的制备及其反应机理分析
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作者 崔炎龙 吴浩 +2 位作者 王钽 秦宏亮 严正人 《当代化工研究》 CAS 2024年第13期178-180,共3页
通过溶剂热方法制备了无水氨苄西林,借助料浆pH变化规律来判断氨苄西林脱水终点,并对氨苄西林脱水机理和脱水动力学模型进行了初步分析。本文主要考察氨苄西林在8%、15%、20%、25%水分异丙醇溶剂中的脱水情况,不同水分情况下料浆pH均出... 通过溶剂热方法制备了无水氨苄西林,借助料浆pH变化规律来判断氨苄西林脱水终点,并对氨苄西林脱水机理和脱水动力学模型进行了初步分析。本文主要考察氨苄西林在8%、15%、20%、25%水分异丙醇溶剂中的脱水情况,不同水分情况下料浆pH均出现了“缓降阶段→骤降阶段→平缓阶段”,平缓阶段pH变化率和ΔpH均可以判断氨苄西林的脱水终点。脱水动力学研究结果表明:氨苄西林脱水表观动力学符合0.5级反应,拟合度R^(2)=0.99746。 展开更多
关键词 氨苄西林脱水 PH 反应动力学
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Decreasing susceptibility of bacteria to ampicillin/sulbactam and third generation cephalosporins in urinary tract infections
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作者 Stefan Vallo Patrick Wirth +4 位作者 Adnan Kukic Omar Nafez Ladislau Neagoe Sebastian Nestler Jon Jones 《Current Urology》 2022年第2期94-98,共5页
Background:Urinary tract infections(UTIs)are among the most common bacterial infections worldwide and have become more difficult to treat over the years.Inappropriate antibiotic use has led to increased antibiotic res... Background:Urinary tract infections(UTIs)are among the most common bacterial infections worldwide and have become more difficult to treat over the years.Inappropriate antibiotic use has led to increased antibiotic resistance.Materials and methods:We examined 1921 urine culture samples from a single hospital and analyzed them for bacterial spectrum and antibiotic susceptibility.We further analyzed changes in the rates of detected bacteria and of the sensitivity of these uropathogens to antibiotics over the years.Results:In our hospital-based analysis,cystitis was the most frequently diagnosed UTI in women(76%)and men(79%).Escherichia coli(48%)was the most commonly identified uropathogen.Samples demonstrated an increase in the proportion of E.coli(p<0.001)and a decrease in Enterococcus faecalis(p<0.001)over the study time period.Antimicrobial susceptibility analysis showed an increase over time in the number of isolates with resistance to ampicillin/sulbactam(p<0.001)and to third-generation cephalosporins cefotaxime(p=0.043)and ceftazidime(p<0.001).Conclusions:Ampicillin/sulbactam and third-generation cephalosporins are antibiotics frequently used in the treatment of UTIs.When selecting an optimal antimicrobial treatment regimen for patients with UTIs,it is imperative to understand regional and timedependent differences in the prevalence of various uropathogens and antimicrobial resistance patterns.Therefore,continuous surveillance of local pathogen and antimicrobial susceptibility patterns for frequently used antibiotics should be prioritized. 展开更多
关键词 ampicillin/sulbactam RESISTANCE Third generation cephalosporin Urinary tract infection
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