[Objective] This study aimed to detect the reliability of LAMP method for detecting Listeria monocytogenes in dairy food. [Method] Based on the sequence of hlyA gene encoding listeriolysin O in Listeria monocytogenes,...[Objective] This study aimed to detect the reliability of LAMP method for detecting Listeria monocytogenes in dairy food. [Method] Based on the sequence of hlyA gene encoding listeriolysin O in Listeria monocytogenes, the LAMP method was established for detecting Listeria monocytogenes in dairy food. [Result] The es- tablished LAMP rapid detection method has .high specificity and sensitivity, which are equivalent to those of real-time fluorescent quantitative PCR. The detection re- sults of Listeria monocytogenes in dairy food by established LAMP method were completely consistent with those by bacterial isolation method. [Conclusion] The de- tection results of Listeria monocytogenes by LAMP method can be directly identified by naked eye, so the established LAMP method was suitable for the detection of Listeria monocytogenes in dairy food in emergency situations.展开更多
In our previous studies, we have proved that neutron irradiation can decrease the single event latch-up （SEL） sensitivity of CMOS SRAM. And one of the key contributions to the multiple cell upset （MCU） is the para...In our previous studies, we have proved that neutron irradiation can decrease the single event latch-up （SEL） sensitivity of CMOS SRAM. And one of the key contributions to the multiple cell upset （MCU） is the parasitic bipolar amplification, it bring us to study the impact of neutron irradiation on the SRAM＇s MCU sensitivity. After the neutron experiment, we test the devices＇ function and electrical parameters. Then, we use the heavy ion fluence to examine the changes on the devices＇ MCU sensitivity pre- and post-neutron-irradiation. Unfortunately, neutron irradiation makes the MCU phenomenon worse. Finally, we use the electric static discharge （ESD） testing technology to deduce the experimental results and find that the changes on the WPM region take the lead rather than the changes on the parasitic bipolar amplification for the 90 nm process.展开更多
基金Supported by Natural Science Foundation of Anhui Province(1508085QC64)~~
文摘[Objective] This study aimed to detect the reliability of LAMP method for detecting Listeria monocytogenes in dairy food. [Method] Based on the sequence of hlyA gene encoding listeriolysin O in Listeria monocytogenes, the LAMP method was established for detecting Listeria monocytogenes in dairy food. [Result] The es- tablished LAMP rapid detection method has .high specificity and sensitivity, which are equivalent to those of real-time fluorescent quantitative PCR. The detection re- sults of Listeria monocytogenes in dairy food by established LAMP method were completely consistent with those by bacterial isolation method. [Conclusion] The de- tection results of Listeria monocytogenes by LAMP method can be directly identified by naked eye, so the established LAMP method was suitable for the detection of Listeria monocytogenes in dairy food in emergency situations.
文摘In our previous studies, we have proved that neutron irradiation can decrease the single event latch-up （SEL） sensitivity of CMOS SRAM. And one of the key contributions to the multiple cell upset （MCU） is the parasitic bipolar amplification, it bring us to study the impact of neutron irradiation on the SRAM＇s MCU sensitivity. After the neutron experiment, we test the devices＇ function and electrical parameters. Then, we use the heavy ion fluence to examine the changes on the devices＇ MCU sensitivity pre- and post-neutron-irradiation. Unfortunately, neutron irradiation makes the MCU phenomenon worse. Finally, we use the electric static discharge （ESD） testing technology to deduce the experimental results and find that the changes on the WPM region take the lead rather than the changes on the parasitic bipolar amplification for the 90 nm process.
