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Targeted treatment of cancer with radiofrequency electromagnetic fields amplitude-modulated at tumor-specific frequencies 被引量:3
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作者 Jacquelyn W.Zimmerman Hugo Jimenez +6 位作者 Michael J.Pennison Ivan Brezovich Desiree Morgan Albert Mudry Frederico P.Costa Alexandre Barbault Boris Pasche 《Chinese Journal of Cancer》 SCIE CAS CSCD 2013年第11期573-581,共9页
In the past century, there have been many attempts to treat cancer with low levels of electric and magnetic fields. We have developed noninvasive biofeedback examination devices and techniques and discovered that pati... In the past century, there have been many attempts to treat cancer with low levels of electric and magnetic fields. We have developed noninvasive biofeedback examination devices and techniques and discovered that patients with the same tumor type exhibit biofeedback responses to the same, precise frequencies. Intrabuccal administration of 27.12 MHz radiofrequency(RF) electromagnetic fields(EMF), which are amplitude-modulated at tumor-specific frequencies, results in long-term objective responses in patients with cancer and is not associated with any significant adverse effects. Intrabuccal administration allows for therapeutic delivery of very low and safe levels of EMF throughout the body as exemplified by responses observed in the femur, liver, adrenal glands, and lungs. In vitro studies have demonstrated that tumor-specific frequencies identified in patients with various forms of cancer are capable of blocking the growth of tumor cells in a tissue- and tumor-specific fashion. Current experimental evidence suggests that tumor-specific modulation frequencies regulate the expression of genes involved in migration and invasion and disrupt the mitotic spindle. This novel targeted treatment approach is emerging as an appealing therapeutic option for patients with advanced cancer given its excellent tolerability. Dissection of the molecular mechanisms accounting for the anti-cancer effects of tumor-specific modulation frequencies is likely to lead to the discovery of novel pathways in cancer. 展开更多
关键词 射频电磁场 肿瘤细胞 癌症患者 频率调节 振幅调制 治疗性 异性 生物反馈
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Long-term radiofrequency electromagnetic fields exposure attenuates cognitive dysfunction in 5×FAD mice by regulating microglial function
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作者 Yeonghoon Son Hye-Jin Park +3 位作者 Ye Ji Jeong Hyung-Do Choi Nam Kim Hae-June Lee 《Neural Regeneration Research》 SCIE CAS CSCD 2023年第11期2497-2503,共7页
We have previously found that long-term effects of exposure to radiofrequency electromagnetic fields in 5xFAD mice with severe late-stage Alzheimer’s disease reduced both amyloid-βdeposition and glial activation,inc... We have previously found that long-term effects of exposure to radiofrequency electromagnetic fields in 5xFAD mice with severe late-stage Alzheimer’s disease reduced both amyloid-βdeposition and glial activation,including microglia.To examine whether this therapeutic effect is due to the regulation of activated microglia,we analyzed mic roglial gene expression profiles and the existence of microglia in the brain in this study.5xFAD mice at the age of 1.5 months were assigned to sham-and radiofrequency electromagnetic fields-exposed groups and then animals were exposed to 1950 MHz radiofrequency electromagnetic fields at a specific absorption rate of 5 W/kg for 2 hours/day and 5 days/week for 6 months.We conducted behavioral tests including the object recognition and Y-maze tests and molecular and histopathological analysis of amyloid precursor protein/a myloid-beta metabolism in brain tissue.We confirmed that radiofrequency electromagnetic field exposure for 6 months ameliorated cognitive impairment and amyloid-βdeposition.The expression levels of Iba1(pan-microglial marker)and colony-stimulating factor 1 receptor(CSF1R;regulates microglial prolife ration)in the hippocampus in 5xFAD mice treated with radiofrequency electromagnetic fields were significantly reduced compared with those of the sham-exposed group.Subsequently,we analyzed the expression levels of genes related to mic rogliosis and microglial function in the radiofrequency electromagnetic fields-exposed group compared to those of a CSF1R inhibitor(PLX3397)-treated group.Both radiofrequency electromagnetic fields and PLX3397 suppressed the levels of genes related to microgliosis(Csf1r,CD68,and Ccl6)and pro-inflammatory cytokine interleukin-1β.N otably,the expression levels of genes related to mic roglial function,including Trem2,Fcgr1α,Ctss,and Spi1,were decreased after long-term radiofrequency electromagnetic field exposure,which was also observed in response to microglial suppression by PLX3397.These results showed that radiofrequency electromagnetic fields ameliorated amyloid-βpathology and cognitive impairment by suppressing amyloid-βdeposition-induced microgliosis and their key regulator,CSF1R. 展开更多
关键词 5×FAD Alzheimer’s disease CSF1R long term exposure microglial function NEUROINFLAMMATION radiofrequency electromagnetic fields therapeutic effect
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Effects of Different Electromagnetic Fields on Circadian Rhythms of Some Haematochemical Parameters in Rats
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作者 LAURA CONTALBRIGO CALOGERO STELLETTA +4 位作者 LAURA FALCIONI STEFANIA CASELLA GIUSEPPE PICCIONE MORANDO SOFFRITTI AND MASSIMO MORGANTE 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2009年第4期348-353,共6页
Objective To investigate the effects of different electromagnetic fields on some haematochemical parameters of circadian rhythms in Sprague-Dawley rats. Methods The study was carried out in 18 male and 18 female rats ... Objective To investigate the effects of different electromagnetic fields on some haematochemical parameters of circadian rhythms in Sprague-Dawley rats. Methods The study was carried out in 18 male and 18 female rats in good health conditions exposed to 50 Hz magnetic sinusoid fields at the intensity of 1000 μT, 100 μT, and 0 μT (control group) respectively, and in 18 male and 18 female rats in good health conditions exposed to 1.8 GHz electromagnetic fields at the intensity of 50 V/m, 25 V/m and 0 V/m (control group), respectively. Following haematochemical parameters for glucose, triglycerides, and total cholesterol were measured. Results Different effects of electromagnetic fields on circadian rhythms of both male and female rats were observed. Different changes occurred in some haematochemical parameters for glucose, triglycerides, and total cholesterol (P〈0.05). Conclusion Exposure to different electromagnetic fields is responsible for the variations of some haematochemical parameters in rats. 展开更多
关键词 Extremely low frequency electromagnetic fields (50 Hz) radiofrequency electromagnetic fields (1.8 GHz) Haematochemical parameters Circadian rhythm Sprague-Dawley rat
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Safety Measurements of Electromagnetic Fields Radiated from Mobile Base Stations in the Western Region of Saudi Arabia
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作者 Mouaaz Nahas Mohammed T. Simsim 《Wireless Engineering and Technology》 2011年第4期221-229,共9页
Over the last decade, detrimental effects of human exposure to electromagnetic radiations have received widespread attention. Communications and Information Technology Commission (CITC) in Saudi Arabia has set guideli... Over the last decade, detrimental effects of human exposure to electromagnetic radiations have received widespread attention. Communications and Information Technology Commission (CITC) in Saudi Arabia has set guidelines and launched a national project—in collaboration with academic and research institutions—to perform measurements of the “electromagnetic fields” (EMFs) radiated from base stations of the mobile phone network. In keeping with its mission to address problems of the local community, Electrical Engineering (EE) department at Umm Al-Qura University (UQU) has been commissioned to conduct this project in the western region of Saudi Arabia. The project is concerned with measuring the strength of EMFs around base stations to locate the Maximum Peak Point (MPP). The aim of measurements is to ensure the site compliance with the CITC guidelines for human exposure to Radiofrequency (RF). This paper discusses possible health consequences caused by human exposure to RF radiations. Furthermore, it sum- marizes the results from 20 selective base stations located in Makkah, Madinah, Jeddah, Taif and Yanbu. The results show that EMF levels at all surveyed sites are far below the National Guidelines for public exposure to RF and that the MPPs are found within the distance range suggested in the CITC guidelines. The paper concludes by providing some useful comments and recommendations for RF sites surveyors. 展开更多
关键词 electromagnetic fieldS Base STATION Maximum PEAK Point radiofrequency Power Density Public LIMIT OCCUPATIONAL LIMIT National Guidelines
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移动电话射频辐射对机体免疫系统影响的研究进展 被引量:5
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作者 路东波 申秀英 许晓路 《浙江师范大学学报(自然科学版)》 CAS 2007年第2期206-210,共5页
从流行病学调查、动物实验和体外研究等方面,综述了近年来国内外学者有关移动电话射频辐射对机体免疫系统影响的研究成果.目前,有关评价电磁场暴露及其危害的流行病学调查依据还很有限,动物实验和体外研究方面的实验结果也缺乏一致性,... 从流行病学调查、动物实验和体外研究等方面,综述了近年来国内外学者有关移动电话射频辐射对机体免疫系统影响的研究成果.目前,有关评价电磁场暴露及其危害的流行病学调查依据还很有限,动物实验和体外研究方面的实验结果也缺乏一致性,所以对移动电话射频辐射是否会影响人体健康尚无定论,主要是因为对射频场的作用机理不明确,导致无法制定测试电磁场效应的统一标准.因此,尽早明确射频场的作用机制从而确定流行病学和实验研究的生物指标,是揭示移动电话射频辐射生物学效应的必要途径. 展开更多
关键词 移动电话 射频辐射 电磁场 免疫系统 淋巴细胞
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低场强射频电磁辐射对幼年大鼠联合型学习记忆功能的影响 被引量:5
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作者 李蕊 罗昌禄 +6 位作者 徐铭婧 赵海玉 李斓 吴冰 姚娟 吴广延 隋建峰 《广西医学》 CAS 2019年第11期1394-1397,共4页
目的探讨低场强射频电磁辐射(RF-EMR)对幼年大鼠联合型学习记忆功能的影响。方法将45只雌性SD大鼠随机分为RF-EMR组、假RF-EMR组及对照组,每组各15只。假RF-EMR组、RF-EMR组大鼠头部分别接受功率密度为0、1500μW/cm^2的低场强RF-EMR,... 目的探讨低场强射频电磁辐射(RF-EMR)对幼年大鼠联合型学习记忆功能的影响。方法将45只雌性SD大鼠随机分为RF-EMR组、假RF-EMR组及对照组,每组各15只。假RF-EMR组、RF-EMR组大鼠头部分别接受功率密度为0、1500μW/cm^2的低场强RF-EMR,连续暴露15d,对照组大鼠不做任何处理。训练大鼠建立经典眨眼条件反射(CR)。在行为训练的第1天、第10天和第15天检测3组大鼠的CR习得率。行为训练结束后的第1天、第10天和第15天,采用高效液相色谱法检测前额叶内谷氨酸及γ-氨基丁酸的含量。结果对照组未出现有效眨眼。RF-EMR组、假RF-EMR组大鼠第10天、第15天的CR习得率较第1天升高(P<0.05);RF-EMR组大鼠第1天、第10天的CR习得率低于假RF-EMR组(P<0.05)。训练结束后第1天,RF-EMR组和假RF-EMR组前额叶内的谷氨酸及γ-氨基丁酸含量、谷氨酸/γ-氨基丁酸比值低于对照组(P<0.05);第10天,RF-EMR组前额叶内的谷氨酸及γ-氨基丁酸含量、谷氨酸/γ-氨基丁酸比值低于假RF-EMR组与对照组(P<0.05)。结论低场强RF-EMR可导致幼年大鼠联合型学习记忆能力下降,其机制可能与低场强射频RF-EMR引起前额叶内神经递质代谢紊乱有关。 展开更多
关键词 射频电磁辐射 低场强 联合型学习记忆 前额叶 谷氨酸 Γ-氨基丁酸 大鼠 幼年
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1.8GHz射频电磁场对大鼠神经元基因表达谱的影响 被引量:1
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作者 张淑芝 姚耿东 +2 位作者 鲁德强 姜槐 许正平 《浙江预防医学》 2009年第7期3-6,共4页
目的了解1.8 GHz移动电话射频电磁场(RF EMF)对大鼠神经细胞基因表达谱的影响,筛选射频电磁场反应基因。方法将原代培养的大鼠神经细随机分为实验组(辐照组)和对照组(假辐照组),经1.8 GHz射频电磁场,SAR为2 W.kg-1,间断辐照24 h(5 min开... 目的了解1.8 GHz移动电话射频电磁场(RF EMF)对大鼠神经细胞基因表达谱的影响,筛选射频电磁场反应基因。方法将原代培养的大鼠神经细随机分为实验组(辐照组)和对照组(假辐照组),经1.8 GHz射频电磁场,SAR为2 W.kg-1,间断辐照24 h(5 min开/10 min关)后,即刻抽提两组细胞的总RNA,用Affymetrix公司的Rat Neurobiology U34 array基因芯片筛选其差异表达基因,寻找可能的RF EMF反应候选基因。结果在1200个候选基因中,筛选出34个差异表达基因,其中上调基因24个,下调基因10个。差异表达基因与多种细胞功能相关(细胞骨架、信号通路、细胞代谢等)。这些基因的变化均小于2倍,但具有统计学意义(P<0.01)。结论1.8 GHz射频辐射影响大鼠神经元多个基因的表达,某些基因的改变提示射频辐射对神经元可能产生不良影响。 展开更多
关键词 射频电磁场 神经元 基因芯片 基因表达谱
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调幅射频电磁场人体辐照实验系统的设计与实现 被引量:1
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作者 蓝茂英 蔡林波 +4 位作者 邓官华 段松 熊威 李晓东 辛学刚 《现代电子技术》 北大核心 2018年第6期104-108,共5页
设计可用于调幅射频电磁场生物效应研究的人体辐照平台,分别从硬件系统部分和软件系统部分两方面进行介绍。硬件系统由射频信号发生模块、功率放大模块、阻抗匹配模块以及发射天线构成,其接收到由软件系统发出的载波、调制频率、调制深... 设计可用于调幅射频电磁场生物效应研究的人体辐照平台,分别从硬件系统部分和软件系统部分两方面进行介绍。硬件系统由射频信号发生模块、功率放大模块、阻抗匹配模块以及发射天线构成,其接收到由软件系统发出的载波、调制频率、调制深度以及功率参数指令后,进而实时产生相应的调幅射频电磁场。实际测量结果表明,设计的调幅射频电磁场人体辐照实验系统具有良好的性能参数,该系统为人体调幅射频电磁场生物效应研究提供一个使用方便、参数可控的电磁辐照平台。 展开更多
关键词 调幅射频电磁场 生物效应 电磁辐照系统 电磁仿真 FDTD 匹配电路
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射频电磁作用下炸药安全性研究进展
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作者 杜斌 姚洪志 +2 位作者 赵凤起 赵团 杨帆 《火炸药学报》 EI CAS CSCD 北大核心 2022年第3期300-307,共8页
分析了利用信息化技术对炸药进行管理的瓶颈和障碍,介绍了国内外近年来在射频电磁场中炸药的安全性研究进展,对炸药从射频电磁场吸收能量的方式进行了探讨,定义了炸药的射频电磁敏感度,分析了在足够的场强和电磁能量作用下,炸药表面或... 分析了利用信息化技术对炸药进行管理的瓶颈和障碍,介绍了国内外近年来在射频电磁场中炸药的安全性研究进展,对炸药从射频电磁场吸收能量的方式进行了探讨,定义了炸药的射频电磁敏感度,分析了在足够的场强和电磁能量作用下,炸药表面或者内部形成热点的可能性。提出未来研究方向是:研究炸药分子在射频电磁场作用下的热力学行为,建立热力学行为与化学反应之间的关联及电磁场作用于炸药的能量耦合模型;研究炸药在射频电磁场作用下的射频电磁敏感度;并探索射频电磁场能量作用下炸药热点的生成机理。 展开更多
关键词 应用化学 射频电磁场 RFID 炸药安全性 热点 射频电磁敏感度
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手机与Wi-Fi辐射对精子发生的影响 被引量:6
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作者 查旭东(综述) 王雯雯(综述) +1 位作者 许松(审校) 商学军(审校) 《中华男科学杂志》 CAS CSCD 北大核心 2019年第5期451-455,共5页
Wi-Fi技术的发展与普及使电磁辐射的暴露日益增加,因而人们对手机及Wi-Fi一类产生的射频电磁场(RF-EMF)可能对健康造成危害的担忧与日俱增,尤其是在当前男性精子浓度下降和不孕不育增加的背景下。电磁辐射的长期暴露不仅使得男性生殖器... Wi-Fi技术的发展与普及使电磁辐射的暴露日益增加,因而人们对手机及Wi-Fi一类产生的射频电磁场(RF-EMF)可能对健康造成危害的担忧与日俱增,尤其是在当前男性精子浓度下降和不孕不育增加的背景下。电磁辐射的长期暴露不仅使得男性生殖器官遭到破坏,还可对精子的数量、形态、活力与卵细胞的结合能力造成负面影响,间接增加了不孕的风险。虽然电磁辐射具有一定危害性,但并非无法避免,其中低强度短时间或间断性的暴露对生殖器官及精子影响甚微,是防护的主要手段。同时许多抗氧化、去自由基试剂,如维生素E、褪黑素等,也可以满足特殊人群的防护需求。本文就电磁辐射对精子的影响以及相应防护方法加以阐述,并对电磁辐射防护发展方向作一综述。 展开更多
关键词 手机 WI-FI 电磁辐射 射频电磁场 精子 生殖健康
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Is Victory over Pancreatic Cancer Possible, with the Help of Tuned Non-Invasive Physiotherapy? A Case Study Says Yes 被引量:1
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作者 Pierre Le Chapellier Badri Matta 《Journal of Cancer Therapy》 2014年第5期460-477,共18页
Could the conventional treatment of pancreatic cancer effectively be supplemented by a low level and non-invasive bio-electromagnetic treatment? A case study, based on the regular exposure of a patient to an electroma... Could the conventional treatment of pancreatic cancer effectively be supplemented by a low level and non-invasive bio-electromagnetic treatment? A case study, based on the regular exposure of a patient to an electromagnetic field, EMF, emitted by a Rife-Bare technology device, suggests so. The plasma confined in a tube of this apparatus emitted radiofrequency solitons. These low level emissions were modulated by an “audio” frequency generator, pre-programmed for the treatment of this disease. After less than two months of exposure to these EMFs, the tumor completely disappeared in approximately two weeks. The explanation of the action mechanism includes a physics aspect relating to the properties of the dissipative soliton which is emitted-absorbed by any non-linear system, a biophysics aspect relating to the coherent structuring of the cellular bath by incident solitons, and finally a biological aspect. The latter is characterized by a critical resonance frequency leading the “unicellular” tumoral cell to adopt a self-destructive behavior. On the other hand EMFs with low level solitons have no effect on the tissues of complex multicellular organisms. 展开更多
关键词 Bioelectromagnetic Cancer of PANCREAS Therapy electromagnetic fields Immune System Living Nature Rife-Bare Soliton radiofrequENCIES
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1800 MHz射频电磁场暴露对大鼠海马GFAP、NCAM和GABA受体表达的影响 被引量:2
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作者 张盛庆宇 舒逍 +6 位作者 吴锡南 李志强 武慧欣 张媛 蒋玉融 杨思佳 木云珍 《昆明医科大学学报》 CAS 2021年第9期13-19,共7页
目的探究1800 MHz射频电磁场对大鼠的体重和海马体胶原纤维酸性蛋白(GFAP)、神经细胞黏附分子(NCBA)和γ-氨基丁酸(GABA)受体表达的影响。方法购买SD大鼠14只,分为暴露组和对照组,暴露组雌雄比例3∶4,对照组雌雄比例4∶3。暴露频率1800 ... 目的探究1800 MHz射频电磁场对大鼠的体重和海马体胶原纤维酸性蛋白(GFAP)、神经细胞黏附分子(NCBA)和γ-氨基丁酸(GABA)受体表达的影响。方法购买SD大鼠14只,分为暴露组和对照组,暴露组雌雄比例3∶4,对照组雌雄比例4∶3。暴露频率1800 MHz,强度0.5 mW/cm2。全身暴露3周,每天12 h(20:00~08:00)。暴露结束后,对2组老鼠分别称重。应用免疫组织化学技术对大鼠海马组织切片GFAP、NCBA、GABA受体进行染色,并测定GFAP、NCBA和GABA受体在海马CA1、CA3和DG区的表达变化。结果暴露组与对照组体重指标无明显不同(P>0.05);暴露组海马CA1区、DG区GFAP的表达下降(P<0.05),其他各组比较无明显不同(P>0.05)。结论在1800 MHz,0.5 mW/cm2射频电磁场使SD大鼠暴露3周,大鼠海马CA1和DG区GFAP表达下降,可能对某些脑部疾病有所影响。 展开更多
关键词 射频电磁场 生理发育 海马
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射频美容电极在人脑中电磁场和比吸收率分布研究 被引量:2
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作者 齐欣哲 逯迈 《中国医学物理学杂志》 CSCD 2020年第4期491-497,共7页
研究在频率40.68 MHz下,使用多极射频电极头对人进行射频美容治疗时在人头部所产生磁场强度、电场强度和比吸收率(Specific Absorption Ratio,SAR)的分布,并将得出的结果与国际非电离辐射防护委员会(ICNIRP)所指定的安全限值进行对比。... 研究在频率40.68 MHz下,使用多极射频电极头对人进行射频美容治疗时在人头部所产生磁场强度、电场强度和比吸收率(Specific Absorption Ratio,SAR)的分布,并将得出的结果与国际非电离辐射防护委员会(ICNIRP)所指定的安全限值进行对比。结果表明,脑组织中磁场强度最大值为0.06 A/m,约为ICNIRP限值的82.19%;电场强度最大值为5.5 V/m,约为INCIRP安全限值的19.64%;SAR最大约为0.03 W/kg,远小于ICNIRP标准的2 W/kg。所有结果均处于ICNIRP安全限值以内,说明射频美容电极所产生的电磁暴露不会对人体造成威胁。 展开更多
关键词 射频美容 人头模型 电磁场 比吸收率
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Effects of Exposure to GSM Mobile Phone Base Station Signals on Salivary Cortisol,Alpha-Amylase,and Immunoglobulin A
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作者 CHRISTOPH AUGNER GERHARD W.HACKER +4 位作者 GERD OBERFELD MATTHIAS FLORIAN WOLFGANG HITZL JORG HUTTER GERNOT PAUSER 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2010年第3期199-207,共9页
Objective The present study aimed to test whether exposure to radiofrequency electromagnetic fields (RF-EMF) emitted by mobile phone base stations may have effects on salivary alpha-amylase, immunoglobulin A (IgA)... Objective The present study aimed to test whether exposure to radiofrequency electromagnetic fields (RF-EMF) emitted by mobile phone base stations may have effects on salivary alpha-amylase, immunoglobulin A (IgA), and cortisol levels. Methods Fifty seven participants were randomly allocated to one of three different experimental scenarios (22 participants to scenario 1, 26 to scenario 2, and 9 to scenario 3). Each participant went through five 50-minute exposure sessions. The main RF-EMF source was a GSM-900-MHz antenna located at the outer wall of the building. In scenarios 1 and 2, the first, third, and fifth sessions were "low" (median power flux density 5.2 μW/m^2) exposure. The second session was "high" (2126.8 μW/m^2), and the fourth session was "medium" (153.6 μW/m^2) in scenario 1, and vice versa in scenario 2. Scenario 3 had four "low" exposure conditions, followed by a "high" exposure condition. Biomedical parameters were collected by saliva samples three times a session. Exposure levels were created by shielding curtains. Results In scenario 3 from session 4 to session 5 (from "low" to "high" exposure), an increase of cortisol was detected, while in scenarios 1 and 2, a higher concentration of alpha-amylase related to the baseline was identified as compared to that in scenario 3. IgA concentration was not significantly related to the exposure. Conclusions RF-EMF in considerably lower field densities than ICNIRP-gnidelines may influence certain psychobiological stress markers. 展开更多
关键词 GSM base stations Mobile phone Salivary IgA Alpha amylase CORTISOL radiofrequency electromagnetic fields (RF-EMF)
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脉冲射频电磁场诱导人羊膜细胞膜受体聚簇及噪声磁场的干预
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作者 干雅平 申秀英 《浙江树人大学学报(自然科学版)》 2007年第4期18-22,共5页
研究经217Hz脉冲调制的GSM1 800MHz模拟移动电话射频电磁场(简称"射频场")对人羊膜FL细胞膜表皮生长因子受体(EGFR)聚簇的可能诱导作用及噪声磁场的干预.将人羊膜FL细胞用比吸收率(SAR)为0.1W·kg-1、0.5W·kg-1、1 W... 研究经217Hz脉冲调制的GSM1 800MHz模拟移动电话射频电磁场(简称"射频场")对人羊膜FL细胞膜表皮生长因子受体(EGFR)聚簇的可能诱导作用及噪声磁场的干预.将人羊膜FL细胞用比吸收率(SAR)为0.1W·kg-1、0.5W·kg-1、1 W·kg-1、2 W·kg-1和4 W·kg-1的射频场,2μT噪声磁场和射频场与噪声磁场叠加的复合场辐照处理15min后,立即将细胞以免疫组化方法标记膜EGFR,用激光共聚焦显微镜观察是否发生聚簇现象(以表皮生长因子(EGF)处理作为阳性对照).SAR值为0.5W·kg-1、1W·kg-1、2W·kg-1和4 W·kg-1的射频场可诱导细胞膜EGFR聚簇.SAR值为0.1W·kg-1时,未见细胞膜出现上述效应.2μT噪声磁场则不能诱导细胞膜EGF受体的聚簇,当2μT噪声磁场与0.5W·kg-1、1W·kg-1和2W·kg-1射频场叠加后,可抑制EGF受体聚簇,但不能完全抑制4W·kg-1射频场诱导的细胞膜EGF受体聚簇.一定强度的1 800 MHz射频场与相应配体一样,能诱导膜EGFR聚簇,其最低作用阈值在0.1 W·kg-1~0.5 W·kg-1之间;噪声磁场对射频场诱导膜受体聚簇的干预作用存在剂量-效应关系. 展开更多
关键词 射频电磁场 表皮生长因子受体 聚簇 噪声磁场
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光照和周期性射频辐射对果蝇睡眠影响的研究 被引量:2
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作者 李志辉 张紫燕 +5 位作者 张虹影 王亚红 章路 唐超 廖艳艳 蔡澎 《军事医学》 CAS 北大核心 2019年第5期357-363,共7页
目的探究周期性射频辐射(RF)对果蝇睡眠行为的影响及调控机制。方法实时连续监测果蝇在光照、RF(1800 MHz,1 W/m2,12 h/d)及黑暗条件下的行为变化,对比分析各条件下果蝇运动和睡眠的异同。收集处理2 h后的样品,分析RF对果蝇生物钟基因pe... 目的探究周期性射频辐射(RF)对果蝇睡眠行为的影响及调控机制。方法实时连续监测果蝇在光照、RF(1800 MHz,1 W/m2,12 h/d)及黑暗条件下的行为变化,对比分析各条件下果蝇运动和睡眠的异同。收集处理2 h后的样品,分析RF对果蝇生物钟基因per、tim、clk、cyc和cry及神经递质关键合酶编码基因hdc、gdh、gad和trh表达水平的影响。结果光照组和RF组的运动和睡眠均表现出与处理周期一致的节律变化,黑暗组节律振幅减弱;与黑暗组相比,光照组果蝇白天和夜晚的睡眠质量均显著上升,RF组果蝇白天和黑暗组的表现一致,但夜间睡眠时间显著延长,睡眠质量上升。短时间暴露下,光照组和RF组果蝇上述5个生物钟基因表达水平全部下调,只是光照组clk、RF组tim下调更显著。此外,光照组和RF组hdc和gad基因表达水平上调,gdh下调,RF组trh基因下调。结论与光同步的周期性RF可能通过影响生物钟和神经递质相关合成基因的表达,进而影响果蝇的睡眠节律、时间及质量,在昼夜节律调控方面可能具有类似光照的功能。 展开更多
关键词 辐射 光照期 射频电磁场 果蝇 睡眠 神经递质 生物钟
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1950 MHz射频电磁场对人脐带间充质干细胞增殖和成骨分化的影响研究 被引量:4
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作者 王甜 郭玲 +5 位作者 安广洲 周艳 张俊平 张克英 郭国祯 丁桂荣 《现代生物医学进展》 CAS 2018年第12期2218-2223,共6页
目的:间充质干细胞(Mesenchymal stem cells,MSCs)具有广阔的临床应用前景,但由于其体外增殖和定向分化等问题,制约了其进一步应用。本研究拟探讨1950MHz射频电磁场(Radio-frequency electromagnetic fields,RF-EMF)对人脐带间充质干细... 目的:间充质干细胞(Mesenchymal stem cells,MSCs)具有广阔的临床应用前景,但由于其体外增殖和定向分化等问题,制约了其进一步应用。本研究拟探讨1950MHz射频电磁场(Radio-frequency electromagnetic fields,RF-EMF)对人脐带间充质干细胞(Human umbilical cord mesenchymal stem cells,hUC-MSCs)增殖和成骨方向分化的影响,以期为MSCs的体外增殖和定向分化提供一条新途径。方法:华通氏胶组织块法分离培养人脐带间充质干细胞,流式细胞仪检测间充质干细胞特异性标志物。选择鉴定后的第3至第6代(P3-P6)hUC-MSCs用于实验。将hUC-MSCs细胞暴露或假暴露于频率为1950 MHz,比吸收率(Specific absorption rate,SAR)分别为0.5,1.0和2.0 W/kg的RF-EMF中,每天暴露1 h(5 min开,10 min关),连续暴露7 d。暴露结束后,流式细胞仪检测细胞周期,免疫荧光检测增殖相关蛋白Ki67表达,连续6天用CCK-8方法检测细胞数。在成骨分化研究中,将P3代的hUC-MSCs随机分为假暴露(sham)组,射频辐射暴露(RF)组,成骨诱导培养基组(Induction medium,OM)和成骨诱导培养基联合射频辐射暴露(OM+RF)组,暴露SAR值为2.0 W/kg,其它参数不变。暴露结束后立即检测细胞的碱性磷酸酶(Alkaline phosphatase,ALP)活性。结果:原代培养的细胞具有MSC典型外观,且表达MSCs特异性表面抗原。与sham组相比,不同SAR值RF暴露后,hUC-MSCs的增殖能力无明显变化,S期细胞比例及Ki67蛋白水平也无显著改变。此外,hUC-MSCs经SAR值为2.0W/kg的RF暴露7 d,与sham组相比其ALP活性无显著变化。与OM组相比,OM+RF组的ALP活性亦无显著改变。结论:华通氏胶组织块法能够培养出纯度较高的间充质干细胞,本实验条件下的1950 MHz射频电磁场对hUC-MSCs的增殖和成骨分化均无显著影响。 展开更多
关键词 射频电磁场 间充质干细胞 细胞增殖 成骨分化
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孕鼠暴露于射频电磁场对子鼠海马超微结构及神经生长因子的影响
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作者 张盛庆宇 杜盼盼 +4 位作者 舒逍 武慧欣 木云珍 吴锡南 张媛 《中华劳动卫生职业病杂志》 CAS CSCD 北大核心 2022年第9期656-660,共5页
目的探究孕鼠暴露于射频电磁场对子代大鼠海马超微结构和肥胖相关蛋白(FTO)及神经生长因子(NGF)水平的影响。方法于2019年9月,选择36只健康7周龄Wistar大鼠,其中雌鼠24只(150~200 g),雄鼠12只(200~250 g)。雌雄鼠以2∶1比例于每晚18∶0... 目的探究孕鼠暴露于射频电磁场对子代大鼠海马超微结构和肥胖相关蛋白(FTO)及神经生长因子(NGF)水平的影响。方法于2019年9月,选择36只健康7周龄Wistar大鼠,其中雌鼠24只(150~200 g),雄鼠12只(200~250 g)。雌雄鼠以2∶1比例于每晚18∶00合笼交配,涂片结果精子阳性为交配成功,当日作为孕0 d。将孕鼠随机分成3组实验组和3组对照组,每组4只。实验组分别给予1800 MHz暴露、Wi-Fi暴露和1800 MHz+Wi-Fi暴露,3个对照组给予虚拟暴露。每天12 h,连续21 d,分三批进行。暴露结束后,各组子鼠饲养到7周,透射电镜观察大脑海马超微结构变化,Western Blot法测定海马内FTO水平,ELISA法测定脑组织中的NGF水平。结果透射电镜观察发现,1800 MHz暴露组子代雌、雄大鼠海马组织胞核轻微收缩,胞质轻微水肿,雄鼠胞核明显不规则。Wi-Fi暴露组子代雌、雄大鼠海马组织胞核收缩较严重,胞膜不规则,胞质出现明显水肿。1800 MHz+Wi-Fi暴露组子代雌、雄大鼠海马组织胞核均严重收缩,核膜不规则,胞质严重水肿。各组间比较,子代雌、雄大鼠FTO水平差异均无统计学意义(P>0.05)。与其他各组比较,1800 MHz+Wi-Fi暴露组子代大鼠海马内的NGF含量明显升高(P<0.05)。结论射频电磁场孕期暴露可能会对子鼠海马组织的超微结构造成影响,且1800 MHz+Wi-Fi叠加暴露可刺激海马内的NGF表达上调。 展开更多
关键词 射频电磁场 孕期 子代大鼠 海马 超微结构 神经生长因子
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