Plant Growth-Prompting Bacteria Influenced Metabolites of <i>Zea mays var. amylacea </i>and <i>Pennisetum americanum p. </i>in a Species-Specific Manner

Poor soil is one of the agricultural world’s principal challenges, inciting the use of chemical fertilizer’s to improve overall soil quality. However, the use of chemical fertilizer has significant and cascading environmental consequences. Therefore, the use of beneficial microbes’ inoculation in treating poor soil is a considerably ecofriendly sustainable solution. In the current study, we supplemented nutrient-deprived soil with plant growth promoting bacteria (PGPB), Pseudomonas fluorescens. The bacterial inoculations of Pseudomonas fluorescenswere added to the poor soil following two days post-sowing of Zea mays var. amylacea and Pennisetumamericanum p. seedlings. Metabolite analyses were conducted two months after treatment for both shoots and roots using nuclear magnetic resonance method (NMR). The data indicated significant changes in 19 metabolites relative to control in both plants shoot and roots. Among these metabolites, 7 were upregulated in roots of Zea mays var. amylacea, and 9 metabolites were upregulated in roots of Pennisetum americanum p. The PGPB enhanced sugars (fructose, glucose, sucrose) and amino acids (glutamate, alanine and succinate) in roots, while down regulating in shoots of Pennisetum americanum p. The Pseudomonas fluorescens induced, predominantly,Aminoacyl-tRNA related metabolite, and Alanine, aspartate and glutamate metabolite biosynthesis in Zea mays var. amylacea), whereas PGPB induced metabolites in Pennisetum americanum p., dominated by up regulated carbohydrate related (starch and sucrose) metabolites. The difference in some metabolic response between the two plants indicated that PGPB influence has a species-specific manner.

85例成人闭合性脑挫伤组织病理学研究

目的研究大脑及脑干脑挫伤后组织病理学变化及与损伤时间的关系,并比较大脑与脑干损伤之间有无差异。方法对85例闭合性颅脑损伤死亡的脑挫伤组织进行病理学GFAP、HSP70、NF200免疫组织化学染色。结果脑损伤早期出现的胞浆嗜酸性肿胀胶质细胞(ECSG)是脑损伤后一种早期应激性反应,挫伤部位淀粉样小体(CA)的增多及噬神经细胞现象也可在脑挫伤后早期形成,而脑干挫伤ECSG发生率高于大脑。中性粒细胞浸润、泡沫细胞及胶质瘢痕形成有一定时序性。结论ECSG在判断生前脑挫伤及推断脑挫伤时间中有重要的参考价值。在ECSG较多的部位,泡沫细胞及CA也较多,三者之间可能存在联系,并提出CA是脑挫伤后反应性产物之一,可用于推断脑损伤时间。

BPH患者中淀粉样变发生情况及临床特征

目的：探讨BPH患者中淀粉样变的发生情况及临床特征，揭示淀粉样变在BPH发生、发展过程中所起的可能作用。方法：采用刚果红染色方法，检测2009-2011年136例在我院行TURP切除标本中淀粉样变的发生情况，分析其与年龄、前列腺体积及其他前列腺疾病的相关性。结果：BPH患者中淀粉样变总阳性表达率为58．8％（80／136）。随着年龄增长，前列腺淀粉样变阳性率显著增加（P〈0．05），前列腺淀粉样变阳性率与前列腺体积无相关性（P〉0．05）；淀粉样变阳性患者中无伴发前列腺癌（PCa）者，而淀粉样变阴性患者中8．9％（5／56）伴发PCa，二者之间差异有统计学意义（P〈0．05）。淀粉样变阳性患者中5．0％（4／80）伴发组织学前列腺炎，阴性患者中5．4％（3／56）伴发组织学前列腺炎，二者之间无统计学意义（P〉0．05）。结论：BPH中淀粉样变发生率随着年龄的增长而增加，但与前列腺体积无关，伴有淀粉样变的BPH·中未发现恶性肿瘤，BPH中淀粉样变的发生与是否伴发前列腺炎无相关性。

家族性淀粉样变多发性神经性损害患者腺体组织存在淀粉样小体

目的报道家族性淀粉样变多发性神经性损害(familial amyloidotic polyneuropathy,FAP)患者腺体组织存在淀粉样小体,用于探讨淀粉样变形成机制。方法 1临床收集转甲状腺素蛋白(transthyretin,TTR)相关淀粉样变患者,利用免疫亲和-质量分析装置法(Matrix assisted laser desorption ionisation time-of-flight mass spectrometry,MALDI-TOF/MS)法测定TTR基因突变类型。2刚果红(Congo red,CR)染色结合偏振光显微镜检查鉴定心脏组织是否发生淀粉样变沉积。3免疫组织化学染色鉴定淀粉样变沉积蛋白类型。4对FAP患者腺体组织进行形态分析以确定淀粉样小体的存在。结果1MALDI-TOF/MS分析结果确定TTR基因突变类型为ATTRVal30Met。2患者心脏组织经CR染色结合偏振光显微镜观察,确定淀粉样变沉积阳性,且沉积于小血管壁和心肌组织间隙。3免疫组织化学染色结果可见TTR免疫反应阳性,提示淀粉样变沉积前蛋白为TTR。4对胰腺组织进行分析发现腺体腔内可见CR染色阳性和绿色荧光淀粉样小体,未见其与TTR抗体存在免疫原性反应。结论 FAP患者胰腺组织发现淀粉样小体,且未发现其与TTR抗体之间存在免疫原性反应。