Mechanism of Simiao Yongan decoction in the treatment of arteriosclerosis obliterans of lower extremity through JAK/STAT signaling pathway

Objective:To predict the effective components,potential targets and pathways of Simiao Yong’an Decoction in the treatment of lower extremity arteriosclerosis obliterans(ASO)based on the method of network pharmacology,and to explore the mechanism of Simiao Yong’an Decoction in treating ASO combined with cell experiment.Methods:TCMSP database was used to screen the active components and targets of Simiao Yong’an decoction,and Genecards and OMIM databases were used to obtain ASO related proteins;PPI network of drug disease target proteins was constructed by string platform;go function and KEGG pathway enrichment of Simiao Yong’an Decoction ASO target were analyzed by David database.The drug target pathway with high correlation with ASO was selected.The model of vascular smooth muscle cell injury(VSMCs)induced by ox LDL was used,and Simiao Yong’an decoction containing pharmaceutical Qing was given to verify the therapeutic effect of Simiao Yong’an Decoction on ox LDL induced VSMCs and its regulation on highly correlated target pathway.Results:A total of 126 active components of Simiao Yong'an Decoction were screened,40 targets of Simiao Yong’an Decoction ASO were selected,99 go biological processes and 48 related signal pathways were related to ASO;the experimental results showed that with the passage of time,Simiao Yongan decoction could significantly inhibit the proliferation of VSMCs(P<0.05);compared with the model group,the percentage of BrdU positive cells in each dose group of Simiao Yong’an decoction was significantly higher than that in the model group In addition,Simiao Yong’an decoction could significantly inhibit the proliferation of VSMCs(P<0.05);in addition,Simiao Yongan decoction could inhibit the levels of IL-6 and IL-1β(P<0.05);significantly inhibit the expression of PCNA,JAK2 and STAT3 mRNA(P<0.05);Conclusions:Simiao Yong’an decoction has the effect of"multi-component,multi-target and multi-channel"in the treatment of ASO.It can inhibit the activation of JAK/STAT signaling pathway,play an anti-inflammatory role and inhibit the proliferation of vascular smooth muscle cells.

Clinical Observation of Zhuang Medicine Crystal Sugar Ye'An Decoction in Treating Senile Skin Pruritus and Its Effects on Serum lgE

Objective:To discuss the specific application of Zhuang Medicine Crystal Sugar Ye'An Decoction in senile skin pruritus and its effects on serum IgE detection level.Methods:90 patients with senile skin pruritus admitted to our hospital from February 2019 to April 2020 were randomly grouped.The general treatment effect,itching,secondary skin lesion severity,sernum IL-6,IgE level and adverse reactions between 45 patients treated with Chlorcyclizine Hydrochloride Tablets(The control group)and 45 patients treated with Crystal Sugar Ye'An Decoction were compared.Results:The total effective rate of the treatment in the observation group was 93.33%,much higher than that in the control group,75.56%.The difference was verified and statistically significant(P<0.05).Before the treatment,the itching area,frequency,duration and severity of secondary skin lesions were at the same level(P>0.05).After the treatment,the above data were all reduced,and the reduction of the observation group was more significant than that of the control group,indicating the difference was statistically significant(P<0.05).Before the treatment,there was no difference in serum IL-6 and IgE test data between the two groups(P>0.05).After the treatment,the tendency all declined,and that of the observation group was more obvious,indicating the difference was statistically significant(P<0.05).During the experiment,no obvious adverse reactions were observed in both groups.Conclusion:In the treatment of patients with senile pruritus,the application of Zhuang Medicine Crystal Sugar Ye’An Decoction is not only safe and reliable,but can promote the improvement of patients'symptoms,adjust the level of inflammatory factors,and enhance clinical efficacy.