In order to enhance phosphorus removal in traditional step-feed anoxic/oxic nitrogen removal process,a modified pilot-scale step-feed anaerobic/anoxic/oxic(SFA 2/O) system was developed,which combined a reactor simila...In order to enhance phosphorus removal in traditional step-feed anoxic/oxic nitrogen removal process,a modified pilot-scale step-feed anaerobic/anoxic/oxic(SFA 2/O) system was developed,which combined a reactor similar to UCT-type configuration and two-stage anoxic/oxic process.The simultaneous nitrogen and phosphorus removal capacities and the potential of denitrifying phosphorus removal,in particular,were investigated with four different feeding patterns using real municipal wastewater.The results showed that the feeding ratios(Q1)in the first stage determined the nutrient removal performance in the SFA 2/O system.The average phosphorus removal efficiency increased from 19.17% to 96.25% as Q1 was gradually increased from run 1 to run 4,but the nitrogen removal efficiency exhibited a different tendency,which attained a maximum 73.61%in run 3 and then decreased to 59.62%in run 4.As a compromise between nitrogen and phosphorus removal,run 3 (Q1=0.45Qtotal) was identified as the optimal and stable case with the maximum anoxic phosphorus uptake rate of 1.58 mg·(g MLSS)-1 ·h-1.The results of batch tests showed that ratio of the anoxic phosphate uptake capacity to the aerobic phosphate uptake capacity increased from 11.96% to 36.85% with the optimal influent feeding ratio to the system in run 3,which demonstrated that the denitrifying polyP accumulating organisms could be accumulated and contributed more to the total phosphorus removal by optimizing the inflow ratio distribution.However,the nitrate recirculation to anoxic zone and influent feeding ratios should be carefully controlled for carbon source saving.展开更多
Transformations of di-n-butyl phthalate(DBP) and di(2-ethylhexyl) phthalate(DEHP) have been investigated in anaerobic/anoxic/oxic(A/A/O) leachate treatment processes. Although the DBP removal processes are different w...Transformations of di-n-butyl phthalate(DBP) and di(2-ethylhexyl) phthalate(DEHP) have been investigated in anaerobic/anoxic/oxic(A/A/O) leachate treatment processes. Although the DBP removal processes are different when the DBP initial concentration is different, the overall system DBP removal efficiencies are high(N 94%).DEHP is much more difficult to remove than DBP. The removal efficiency of DEHP is approximately 75%–78%.The results of mass balance calculations indicate that approximately 33.7%–50.7% of the DBP is degraded by the activated sludge, 48.9%–64.9% accumulates in the system, and 0.4%–1.4% is contained in the final effluent. Approximately 15.0%–19.0% of the DEHP is degraded by activated microcosms, 75.8%–79.0% accumulates in the system, and 5.2%–6.0% is contained in the final effluent. Biodegradation and adsorption to the activated sludge are the main mechanisms for DBP removal and adsorption to the activated sludge is the main mechanism for DEHP removal. The different removal mechanisms of the two PAEs may be related to their different molecular structures. However, PAEs are not really removed when they adsorb onto the sludge. Therefore, methods for decreasing PAEs adsorption and increasing the biodegradation efficiencies of the leachate treatment processes should be further investigated.展开更多
Microbial fuel cell (MFC) embedded in anaerobic-anoxic-oxic (A2/O) process has positive effects on wastewater treatment, which can enhance the efficiencies of pollutants’ removal, along with electricity production. B...Microbial fuel cell (MFC) embedded in anaerobic-anoxic-oxic (A2/O) process has positive effects on wastewater treatment, which can enhance the efficiencies of pollutants’ removal, along with electricity production. But the electricity generation performance and its optimization of MFC embedded in A2O process still needs to be further investigated. In this study, in order to optimize the contaminants removal and electricity production of the MFC-A2/O reactor, a lab-scale corridor-style MFC-A2/O reactor, which could simulate the practical A2/O biological reactor better, was designed and operated. The removal efficiencies of chemical oxygen demand, total nitrogen and total phosphorus were continuously monitored so as the electricity generation. In addition, the influences of the structural parameters’ changes of MFC on the output voltage, including electrode material, the directly connected area and the distance between electrodes, were also studied. The results elucidated that the effluent quality of A2/O reactor could be improved when MFC was embedded, and all the investigated structural factors were closely related to the electricity generation performance of MFC to some extent.展开更多
The anaerobic/anoxic/oxic（A2O） process is globally one of the widely used biological sewage treatment processes. This is the first report of a metagenomic analysis using Illumina sequencing of full-scale A2O sludge ...The anaerobic/anoxic/oxic（A2O） process is globally one of the widely used biological sewage treatment processes. This is the first report of a metagenomic analysis using Illumina sequencing of full-scale A2O sludge from a municipal sewage treatment plant.With more than 530,000 clean reads from different taxa and metabolic categories, the metagenome results allow us to gain insight into the functioning of the biological community of the A2O sludge. There are 51 phyla and nearly 900 genera identified from the A2O activated sludge ecosystem. Proteobacteria, Bacteroidetes, Nitrospirae and Chloroflexi are predominant phyla in the activated sludge, suggesting that these organisms play key roles in the biodegradation processes in the A2O sewage treatment system.Nitrospira, Thauera, Dechloromonas and Ignavibacterium, which have abilities to metabolize nitrogen and aromatic compounds, are most prevalent genera. The percent of nitrogen and phosphorus metabolism in the A2O sludge is 2.72% and 1.48%, respectively. In the current A2O sludge, the proportion of Candidatus Accumulibacter is 1.37%, which is several times more than that reported in a recent study of A2O sludge. Among the four processes of nitrogen metabolism, denitrification related genes had the highest number of sequences（76.74%）, followed by ammonification（15.77%）, nitrogen fixation（3.88%） and nitrification（3.61%）. In phylum Planctomycetes, four genera（Planctomyces, Pirellula, Gemmata and Singulisphaera） are included in the top 30 abundant genera, suggesting the key role of ANAMMOX in nitrogen metabolism in the A2O sludge.展开更多
In this study,an anaerobic/anoxic/oxic(A^(2)O)wastewater treatment process was implemented to treat domestic wastewater with short-term atrazine addition.The results provided an evaluation on the effects of an acciden...In this study,an anaerobic/anoxic/oxic(A^(2)O)wastewater treatment process was implemented to treat domestic wastewater with short-term atrazine addition.The results provided an evaluation on the effects of an accidental pollution on the operation of a wastewater treatment plant(WWTP)in relation to Chemical Oxygen Demand(COD)and biological nutrient removal.Domestic wastewater with atrazine addition in 3 continuous days was treated when steady biological nutrient removal was achieved in the A^(2)O process.The concentrations of atrazine were 15,10,and 5 mg%L–1 on days 1,2 and 3,respectively.The results showed that atrazine addition did not affect the removal of COD.The specific NH4þoxidation rate and NO3–reduction rate decreased slightly due to the short-term atrazine addition.However,it did not affect the nitrogen removal due to the high nitrification and denitrification capacity of the system.Total nitrogen(TN)removal was steady,and more than 70%was removed during the period studied.The phosphorus removal rate was not affected by the short-term addition of atrazine under the applied experimental conditions.However,more poly-hydroxy-alkanoate(PHA)was generated and utilized during atrazine addition.The results of the oxygen uptake rate(OUR)showed that the respiration of nitrifiers decreased significantly,while the activity of carbon utilizers had no obvious change with the atrazine addition.Atrazine was not removed with the A^(2)O process,even via absorption by the activated sludge in the process of the short-term addition of atrazine.展开更多
基金Supported by the Project of Beijing Science and Technology Committee (D07050601500000)the National Key Science and Technology Special Projects (2008ZX07317-007-105)
文摘In order to enhance phosphorus removal in traditional step-feed anoxic/oxic nitrogen removal process,a modified pilot-scale step-feed anaerobic/anoxic/oxic(SFA 2/O) system was developed,which combined a reactor similar to UCT-type configuration and two-stage anoxic/oxic process.The simultaneous nitrogen and phosphorus removal capacities and the potential of denitrifying phosphorus removal,in particular,were investigated with four different feeding patterns using real municipal wastewater.The results showed that the feeding ratios(Q1)in the first stage determined the nutrient removal performance in the SFA 2/O system.The average phosphorus removal efficiency increased from 19.17% to 96.25% as Q1 was gradually increased from run 1 to run 4,but the nitrogen removal efficiency exhibited a different tendency,which attained a maximum 73.61%in run 3 and then decreased to 59.62%in run 4.As a compromise between nitrogen and phosphorus removal,run 3 (Q1=0.45Qtotal) was identified as the optimal and stable case with the maximum anoxic phosphorus uptake rate of 1.58 mg·(g MLSS)-1 ·h-1.The results of batch tests showed that ratio of the anoxic phosphate uptake capacity to the aerobic phosphate uptake capacity increased from 11.96% to 36.85% with the optimal influent feeding ratio to the system in run 3,which demonstrated that the denitrifying polyP accumulating organisms could be accumulated and contributed more to the total phosphorus removal by optimizing the inflow ratio distribution.However,the nitrate recirculation to anoxic zone and influent feeding ratios should be carefully controlled for carbon source saving.
基金funded by the National Natural Science Foundation of China(51678531,51878617).
文摘Transformations of di-n-butyl phthalate(DBP) and di(2-ethylhexyl) phthalate(DEHP) have been investigated in anaerobic/anoxic/oxic(A/A/O) leachate treatment processes. Although the DBP removal processes are different when the DBP initial concentration is different, the overall system DBP removal efficiencies are high(N 94%).DEHP is much more difficult to remove than DBP. The removal efficiency of DEHP is approximately 75%–78%.The results of mass balance calculations indicate that approximately 33.7%–50.7% of the DBP is degraded by the activated sludge, 48.9%–64.9% accumulates in the system, and 0.4%–1.4% is contained in the final effluent. Approximately 15.0%–19.0% of the DEHP is degraded by activated microcosms, 75.8%–79.0% accumulates in the system, and 5.2%–6.0% is contained in the final effluent. Biodegradation and adsorption to the activated sludge are the main mechanisms for DBP removal and adsorption to the activated sludge is the main mechanism for DEHP removal. The different removal mechanisms of the two PAEs may be related to their different molecular structures. However, PAEs are not really removed when they adsorb onto the sludge. Therefore, methods for decreasing PAEs adsorption and increasing the biodegradation efficiencies of the leachate treatment processes should be further investigated.
文摘Microbial fuel cell (MFC) embedded in anaerobic-anoxic-oxic (A2/O) process has positive effects on wastewater treatment, which can enhance the efficiencies of pollutants’ removal, along with electricity production. But the electricity generation performance and its optimization of MFC embedded in A2O process still needs to be further investigated. In this study, in order to optimize the contaminants removal and electricity production of the MFC-A2/O reactor, a lab-scale corridor-style MFC-A2/O reactor, which could simulate the practical A2/O biological reactor better, was designed and operated. The removal efficiencies of chemical oxygen demand, total nitrogen and total phosphorus were continuously monitored so as the electricity generation. In addition, the influences of the structural parameters’ changes of MFC on the output voltage, including electrode material, the directly connected area and the distance between electrodes, were also studied. The results elucidated that the effluent quality of A2/O reactor could be improved when MFC was embedded, and all the investigated structural factors were closely related to the electricity generation performance of MFC to some extent.
基金supported by the National Natural Science Foundation of China (No. 41430643)
文摘The anaerobic/anoxic/oxic（A2O） process is globally one of the widely used biological sewage treatment processes. This is the first report of a metagenomic analysis using Illumina sequencing of full-scale A2O sludge from a municipal sewage treatment plant.With more than 530,000 clean reads from different taxa and metabolic categories, the metagenome results allow us to gain insight into the functioning of the biological community of the A2O sludge. There are 51 phyla and nearly 900 genera identified from the A2O activated sludge ecosystem. Proteobacteria, Bacteroidetes, Nitrospirae and Chloroflexi are predominant phyla in the activated sludge, suggesting that these organisms play key roles in the biodegradation processes in the A2O sewage treatment system.Nitrospira, Thauera, Dechloromonas and Ignavibacterium, which have abilities to metabolize nitrogen and aromatic compounds, are most prevalent genera. The percent of nitrogen and phosphorus metabolism in the A2O sludge is 2.72% and 1.48%, respectively. In the current A2O sludge, the proportion of Candidatus Accumulibacter is 1.37%, which is several times more than that reported in a recent study of A2O sludge. Among the four processes of nitrogen metabolism, denitrification related genes had the highest number of sequences（76.74%）, followed by ammonification（15.77%）, nitrogen fixation（3.88%） and nitrification（3.61%）. In phylum Planctomycetes, four genera（Planctomyces, Pirellula, Gemmata and Singulisphaera） are included in the top 30 abundant genera, suggesting the key role of ANAMMOX in nitrogen metabolism in the A2O sludge.
基金the National Key Science and Technology Special Projects(No.2008ZX07209-003)Funding Project for Academic Human Resources Development in Institutions of Higher Learning under the Jurisdiction of Beijing Municipality(No.PHR20090502).
文摘In this study,an anaerobic/anoxic/oxic(A^(2)O)wastewater treatment process was implemented to treat domestic wastewater with short-term atrazine addition.The results provided an evaluation on the effects of an accidental pollution on the operation of a wastewater treatment plant(WWTP)in relation to Chemical Oxygen Demand(COD)and biological nutrient removal.Domestic wastewater with atrazine addition in 3 continuous days was treated when steady biological nutrient removal was achieved in the A^(2)O process.The concentrations of atrazine were 15,10,and 5 mg%L–1 on days 1,2 and 3,respectively.The results showed that atrazine addition did not affect the removal of COD.The specific NH4þoxidation rate and NO3–reduction rate decreased slightly due to the short-term atrazine addition.However,it did not affect the nitrogen removal due to the high nitrification and denitrification capacity of the system.Total nitrogen(TN)removal was steady,and more than 70%was removed during the period studied.The phosphorus removal rate was not affected by the short-term addition of atrazine under the applied experimental conditions.However,more poly-hydroxy-alkanoate(PHA)was generated and utilized during atrazine addition.The results of the oxygen uptake rate(OUR)showed that the respiration of nitrifiers decreased significantly,while the activity of carbon utilizers had no obvious change with the atrazine addition.Atrazine was not removed with the A^(2)O process,even via absorption by the activated sludge in the process of the short-term addition of atrazine.
