The Use of Repeated Measures Analysis of Variance to Study the Effect of Phlegm-Heat Syndrome on Neurological Deficits in Patients with Stroke~

Objective： To explore the effect of phlegm-heat syndrome on the degree of neurological deficit and provide some data support for the correct recognition of the relationship between phlegm-heat syndrome and neurological deficits in stroke patients. Methods： Clinical information were collected on 294 patients with acute ischemic stroke （AIS） whose syndrome and National Institute of Health stroke scale （NIHSS） score were checked at baseline （within the first 3-day admission） and at 7, 14, 28, and 90 days after admission to our clinical research centre. We explored the effect of phlegm-heat syndrome on the degree of neurological deficit following stroke by applying a repeated measures analysis of variance. Results： Stroke patients with phlegm-heat syndrome had higher NIHSS score than patients without the syndrome （P〈0.01）, and there appeared to be a decrease in NIHSS score over time in all patients （P〈0.01）. Conduslons： Phlegm-heat syndrome in patients who have suffered stroke has an effect on the degree of neurological deficiency. Disappearance of phlegm-heat syndrome may improve the degree of neurological deficit observed in stroke patients.

Influence of acupuncture on neural movement function in rats with middle cerebral artery occlusion-A randomized controlled trial

OBJECTIVES:To observe recovery in movement function in rats with middle cerebral artery occlusion(MCAO) after acupuncture treatment.METHODS:According to the randomized and controlled principle 1384 rats were divided into the ba-sic control group(including the normal,sham,model control,model without intervention,Nimodipine,and para-Renzhong groups) and the acupuncture group(including the Neiguan(PC 6),Weizhong(BL 40),Sanyinjiao(SP 6),Chize(LU 5),Renzhong(GV 6) and non-acupoint groups).MCAO was modeled by Zea-longa's thread ligation and rats with scores of 1-3,as assessed by Zausinger's six-point method,were used in this study.Moreover,in the acupuncture group each acupoint was set with 12 different parameters by the orthogonal intersection method,resulting in 78 groups with 18 rats per group.The rats were treated by acupuncture once every 12 h for a total of six sessions and neurobehavioral scores were measured after each session.The neurobehavioral scores were compared by one-way ANOVA using the statistical software SPSS 17.0.RESULTS:After acupuncture therapy the mean neurobehavioral scores in MCAO rats increased gradually at each time point with a significant difference among the six scores,but with no significant differences between the fourth(48 h) and the fifth score(60 h),and between the fifth(60 h) and the sixth(72 h) score(P > 0.05).CONCLUSIONS:MCAO rats gradually recovered movement function over multiple acupuncture sessions.After the fouth acupuncture session(48 h),the neurobehavioral scores of rats with cerebral infarction remained stable.Acupuncture treatment had a reliable curative effect on movement function in cerebral infarction rats.