The objective of this study was to verify the supposition that efflux might be involved in the drug resistance of Riemerella anatipestifer isolates. Two broad-spectrum effiux pump inhibitors, carbonyl cyanide 3-chloro...The objective of this study was to verify the supposition that efflux might be involved in the drug resistance of Riemerella anatipestifer isolates. Two broad-spectrum effiux pump inhibitors, carbonyl cyanide 3-chlorophenylhydrazone （CCCP） and Phe-Arg-β-naphthylamide （PAL3N）, on the contribution of minimum inhibitory concentrations of amikacin, streptomycin, chloramphenicol, tetracycline, ceftdaxone, ceftazidime, nalidixic acid, levofloxacin, enrofloxacin, as well as ciprofloxacin against 69 clinical R. anatipestifer isolates were investigated. We first reported that the two efflux pump inhibitors could restore the antimicrobial susceptibility of R. anatipestiferisolates. It is suggested that active efflux system is possible to be linked with the development of resistance in R. anatipestifer isolates.展开更多
It is a very important significance to explore the bacterial typing methods rapidly, accurately and understand the genetic relation between isolates for controlling effectively the disease and preventing the dissemina...It is a very important significance to explore the bacterial typing methods rapidly, accurately and understand the genetic relation between isolates for controlling effectively the disease and preventing the dissemination and diffusion of the pathogen. The changes in the genetic material nucleotide sequences result in variation and evolution of bacteria, the development of molecular biology and genomics make typing of bacteria from phenotype to molecular typing. Riemerella anatipestifer is the causative agent of polyserositis of ducks and geese. We studied 54 isolates of Riemerella anatipestifer from Guangdong in China by multicolor sequence typing (MLST). The result showed that 54 isolates of Riemerella anatipestifer were divided into 14 STs, and among E3, b12xiao and Cb1 three isolates have with independent of type STs. It was found that there was high homology of RA and low genetic variability in the same area. The synonymous mutation and non synonymous mutation rate: dN/dS in seven housekeeping genes was lower than 0.25. Cluster analysis showed that the 54 isolates were clustered into 5 groups. eBRUST analysis showed that all isolates were clustered into one Group. It also proved that the genetic relationship was very closed in duck plague pathogen in this area;the source may be the same ancestors.展开更多
基金supported by the Program for Changjiang Scholars and Innovative Research Team in University of Ministry of Education of China (IRT13063)the National Natural Science Foundation of China (31072169)
文摘The objective of this study was to verify the supposition that efflux might be involved in the drug resistance of Riemerella anatipestifer isolates. Two broad-spectrum effiux pump inhibitors, carbonyl cyanide 3-chlorophenylhydrazone （CCCP） and Phe-Arg-β-naphthylamide （PAL3N）, on the contribution of minimum inhibitory concentrations of amikacin, streptomycin, chloramphenicol, tetracycline, ceftdaxone, ceftazidime, nalidixic acid, levofloxacin, enrofloxacin, as well as ciprofloxacin against 69 clinical R. anatipestifer isolates were investigated. We first reported that the two efflux pump inhibitors could restore the antimicrobial susceptibility of R. anatipestiferisolates. It is suggested that active efflux system is possible to be linked with the development of resistance in R. anatipestifer isolates.
文摘It is a very important significance to explore the bacterial typing methods rapidly, accurately and understand the genetic relation between isolates for controlling effectively the disease and preventing the dissemination and diffusion of the pathogen. The changes in the genetic material nucleotide sequences result in variation and evolution of bacteria, the development of molecular biology and genomics make typing of bacteria from phenotype to molecular typing. Riemerella anatipestifer is the causative agent of polyserositis of ducks and geese. We studied 54 isolates of Riemerella anatipestifer from Guangdong in China by multicolor sequence typing (MLST). The result showed that 54 isolates of Riemerella anatipestifer were divided into 14 STs, and among E3, b12xiao and Cb1 three isolates have with independent of type STs. It was found that there was high homology of RA and low genetic variability in the same area. The synonymous mutation and non synonymous mutation rate: dN/dS in seven housekeeping genes was lower than 0.25. Cluster analysis showed that the 54 isolates were clustered into 5 groups. eBRUST analysis showed that all isolates were clustered into one Group. It also proved that the genetic relationship was very closed in duck plague pathogen in this area;the source may be the same ancestors.
