Translation is an activity aimed at transferring the source language to the target language,related to many aspects involving culture,society,history and so on.The translator plays a critical role in translation pract...Translation is an activity aimed at transferring the source language to the target language,related to many aspects involving culture,society,history and so on.The translator plays a critical role in translation practice.Xu Jun considers"translator’s subjectivity should be reflected to be artistic personal consciousness in translated works."Translator’s differences in age,gender,personal traits,educational level and other aspects make translation artistry completely divergent.Therefore,translator’s subjectivity shoulders great significances on the success of literary translation,which isn’t arbitrary,out of control,must be conducted in line with essence of the original work.This paper focuses on the gender difference of translator’s subjectivity in literary translation,hoping to find a way of eliminating the influence of it in the translation work.展开更多
Autophagy is an evolutionarily well conserved process in which the cellular components including damaged subcellular organelles are engulfed in autophagosome and eventually delivered to lysosome for degradation.It has...Autophagy is an evolutionarily well conserved process in which the cellular components including damaged subcellular organelles are engulfed in autophagosome and eventually delivered to lysosome for degradation.It has been well studied that autophagy is closely implicated in many diseases such as cancer and neurodegenerative diseases.Therefore,the autophagy-lysosome pathway becomes an attractive target in developing novel therapeutic approaches.In the past several years,we have studied the effects of several natural products on the autophagy-lysosome pathway:(i)Andrographolide(Andro),a diterpenoid lactone isolated from an herbal plant Andrographispaniculata,is capable of suppressing autophagy and sensitizing cisplatin-mediated apoptosis in human cancer cells,via blockage of autophagosome-lysosome fusion;(ii)(-)-Epigallocatechin-3-gallate(EGCG),an important green tea polyphenol,induces lysosomal membrane permeabilization(LMP)and eventually leads to lysosome-associated cell death;and(iii)Artesunate(ART),an analog of artemisinin,an anti-malaria drug,is able to kill cancer cells via enhancing lysosomal function and induction of lysosomal degradation of ferritin.Collectively,our findings reveal novel insights into the molecular mechanisms underlying the anti-cancer properties of those natural compounds and demonstrate that targeting the autophagy-lysosome pathway could serve as a new strategy in developing anti-cancer therapeutic agents.展开更多
Most drugs exert pharmacological effects through interaction with their target proteins.Therefore,drug target identification is a crucial step towards the understanding of the mechanism of drug action.It is also imper...Most drugs exert pharmacological effects through interaction with their target proteins.Therefore,drug target identification is a crucial step towards the understanding of the mechanism of drug action.It is also imperative to study the pharmacodynamics of a known drug,with an aim to discover the potentially unrevealed actions and thus refine its future clinical applications.Currently,drug-target identification is either through in vitro affinity chromatography-based approaches or in vivo activity-based protein profiling(ABPP)approaches.However,these approaches generally face difficulties discriminating specific drug targets from non-specific ones.To address this issue,we have come up with a strategy by coupling iTRAQTM(isobaric tags for relative and absolute quantitation)quantitative proteomics approach with clickable ABPP,to specifically and compre hensively identify drug targets in live cells.Using this approach,we identified the protein targets of andrographolide,a natural product with known anti-inflammation and anti-cancer effects,in live cancer cells.The identified target list not only confirmed the known functions of the drug but also revealed its potential novel application as a tumor metastasis inhibitor.We have also used this strategy,combining with a cleavable probe to identify the protein targets of aspirin and its binding sites.Our results revealed the roles of aspirin ininhibition of protein synthesis and induction of autophagy,which have been functionally validated.Our strategy is widely applicable to the identification of protein targets of covalent drugs.展开更多
文摘Translation is an activity aimed at transferring the source language to the target language,related to many aspects involving culture,society,history and so on.The translator plays a critical role in translation practice.Xu Jun considers"translator’s subjectivity should be reflected to be artistic personal consciousness in translated works."Translator’s differences in age,gender,personal traits,educational level and other aspects make translation artistry completely divergent.Therefore,translator’s subjectivity shoulders great significances on the success of literary translation,which isn’t arbitrary,out of control,must be conducted in line with essence of the original work.This paper focuses on the gender difference of translator’s subjectivity in literary translation,hoping to find a way of eliminating the influence of it in the translation work.
文摘Autophagy is an evolutionarily well conserved process in which the cellular components including damaged subcellular organelles are engulfed in autophagosome and eventually delivered to lysosome for degradation.It has been well studied that autophagy is closely implicated in many diseases such as cancer and neurodegenerative diseases.Therefore,the autophagy-lysosome pathway becomes an attractive target in developing novel therapeutic approaches.In the past several years,we have studied the effects of several natural products on the autophagy-lysosome pathway:(i)Andrographolide(Andro),a diterpenoid lactone isolated from an herbal plant Andrographispaniculata,is capable of suppressing autophagy and sensitizing cisplatin-mediated apoptosis in human cancer cells,via blockage of autophagosome-lysosome fusion;(ii)(-)-Epigallocatechin-3-gallate(EGCG),an important green tea polyphenol,induces lysosomal membrane permeabilization(LMP)and eventually leads to lysosome-associated cell death;and(iii)Artesunate(ART),an analog of artemisinin,an anti-malaria drug,is able to kill cancer cells via enhancing lysosomal function and induction of lysosomal degradation of ferritin.Collectively,our findings reveal novel insights into the molecular mechanisms underlying the anti-cancer properties of those natural compounds and demonstrate that targeting the autophagy-lysosome pathway could serve as a new strategy in developing anti-cancer therapeutic agents.
文摘Most drugs exert pharmacological effects through interaction with their target proteins.Therefore,drug target identification is a crucial step towards the understanding of the mechanism of drug action.It is also imperative to study the pharmacodynamics of a known drug,with an aim to discover the potentially unrevealed actions and thus refine its future clinical applications.Currently,drug-target identification is either through in vitro affinity chromatography-based approaches or in vivo activity-based protein profiling(ABPP)approaches.However,these approaches generally face difficulties discriminating specific drug targets from non-specific ones.To address this issue,we have come up with a strategy by coupling iTRAQTM(isobaric tags for relative and absolute quantitation)quantitative proteomics approach with clickable ABPP,to specifically and compre hensively identify drug targets in live cells.Using this approach,we identified the protein targets of andrographolide,a natural product with known anti-inflammation and anti-cancer effects,in live cancer cells.The identified target list not only confirmed the known functions of the drug but also revealed its potential novel application as a tumor metastasis inhibitor.We have also used this strategy,combining with a cleavable probe to identify the protein targets of aspirin and its binding sites.Our results revealed the roles of aspirin ininhibition of protein synthesis and induction of autophagy,which have been functionally validated.Our strategy is widely applicable to the identification of protein targets of covalent drugs.
