期刊文献+
共找到3,416篇文章
< 1 2 171 >
每页显示 20 50 100
Angiotensin Ⅱ vaccine promising for patients with chronic heart failure
1
作者 CHEN Yang-xin YAO You-fie NIE Ru-qiong ZHOU Shu-xian WANG Jing-feng 《Chinese Medical Journal》 SCIE CAS CSCD 2009年第1期106-109,共4页
Chronic heart failure (CHF), as the end-stage presentation of all kinds of heart diseases, is a major public health problem as well issue. There are more than 5 as a pressing public policy million patients diagnosed... Chronic heart failure (CHF), as the end-stage presentation of all kinds of heart diseases, is a major public health problem as well issue. There are more than 5 as a pressing public policy million patients diagnosed with CHF in USA alone and approximately 550 000 new cases appear per year. About 0.4%-2% of the European population is affected by symptomatic heart failure. Hence heart failure is the leading cause of hospitalization especially in older people around the world. 展开更多
关键词 angiotensin vaccine chronic heart failure
原文传递
血清α1-抗胰蛋白酶、血管紧张素-Ⅱ与获得性免疫缺陷综合征患者预后的关系
2
作者 黄左宇 朱晓红 +2 位作者 陆雪峰 邹美银 曹力 《国际检验医学杂志》 CAS 2024年第5期549-553,共5页
目的探讨血清α1-抗胰蛋白酶(α1-AT)、血管紧张素-Ⅱ(Ang-Ⅱ)与获得性免疫缺陷综合征(AIDS)患者预后的关系。方法将该院2019年5月至2022年5月收治的97例首诊AIDS患者纳入研究作为研究组,另选取同期于该院进行体检的健康者97例作为对照... 目的探讨血清α1-抗胰蛋白酶(α1-AT)、血管紧张素-Ⅱ(Ang-Ⅱ)与获得性免疫缺陷综合征(AIDS)患者预后的关系。方法将该院2019年5月至2022年5月收治的97例首诊AIDS患者纳入研究作为研究组,另选取同期于该院进行体检的健康者97例作为对照组。根据病历收集患者临床资料。对纳入研究者进行α1-AT、Ang-Ⅱ水平检测,并进行分组比较。对纳入研究的AIDS患者进行为期1年的随访,观察患者预后情况,并比较不同预后患者的α1-AT、Ang-Ⅱ水平。采用单因素及多因素Logistic回归分析影响AIDS患者预后的因素。用受试者工作特征(ROC)曲线分析α1-AT、Ang-Ⅱ水平对患者预后的预测效能。结果研究组血清α1-AT和Ang-Ⅱ水平高于对照组(P<0.05)。AIDS患者1年内的预后不良发生率为23.71%(23/97)。预后不良患者血清α1-AT和Ang-Ⅱ水平高于预后良好患者(P<0.05)。单因素分析显示,预后不良患者C反应蛋白(CRP)水平、淋巴细胞计数水平、合并淋巴瘤者所占比例均高于预后良好患者,清蛋白(ALB)水平低于预后良好患者(P<0.05)。多因素Logistic回归分析显示,合并淋巴瘤(OR=2.087)、高α1-AT水平(OR=2.611)、高Ang-Ⅱ水平(OR=2.138)是影响患者预后的独立危险因素(P<0.05)。ROC曲线分析显示,α1-AT预测AIDS患者预后的曲线下面积(AUC)为0.778,Ang-Ⅱ预测的AUC为0.798,α1-AT联合Ang-Ⅱ预测的AUC为0.918。结论α1-AT和Ang-Ⅱ在AIDS患者血清中水平异常升高,而且与患者预后有关,是影响患者预后的独立危险因素。α1-AT和Ang-Ⅱ联合检测可有效预测患者预后。 展开更多
关键词 获得性免疫缺陷综合征 Α1-抗胰蛋白酶 血管紧张素- 预后评估 预测价值
下载PDF
鲤疱疹病毒二型(CyHV-Ⅱ)疫苗研究进展
3
作者 吴琳娇 孙丽芳 +4 位作者 朱春华 张红 董盼盼 陈磊清 吴允昆 《中国农业科技导报》 CAS CSCD 北大核心 2024年第2期137-144,共8页
鲤疱疹病毒Ⅱ型(Cyprinid herpesvirus Ⅱ,CyHV-Ⅱ)对任何发育时期的金鱼、鲫鱼及其杂交品种均具有感染能力,能够引起金鱼疱疹病(goldfish haematopoietic necrosis, GFHN),是一种传染性强且死亡率极高的疫病,给我国金鱼和鲫鱼养殖业造... 鲤疱疹病毒Ⅱ型(Cyprinid herpesvirus Ⅱ,CyHV-Ⅱ)对任何发育时期的金鱼、鲫鱼及其杂交品种均具有感染能力,能够引起金鱼疱疹病(goldfish haematopoietic necrosis, GFHN),是一种传染性强且死亡率极高的疫病,给我国金鱼和鲫鱼养殖业造成巨大经济损失,鱼用疫苗的研发是预防和治疗该疫病最有效的办法。综述了CyHV-Ⅱ灭活疫苗、亚单位疫苗、核酸疫苗、活载体疫苗和纳米递送疫苗的研究进展,并对鱼用疫苗纳米材料的应用前景进行了探讨,以期为金鱼疱疹病害防治和鱼用疫苗研究提供新技术方法和新思路策略。 展开更多
关键词 鲤疱疹病毒二型(CyHV-) 免疫途径 纳米材料 疫苗类型
下载PDF
基于转录组学及表观遗传组学筛选与血管紧张素-Ⅱ相关的胸主动脉瘤/夹层诊断标志物
4
作者 刘冰清 董鉴筠 +3 位作者 沙梦瑶 侯月 刘丹伶奕 刘畅 《北华大学学报(自然科学版)》 CAS 2024年第6期763-769,共7页
目的探讨基于转录组学及表观遗传组学筛选与血管紧张素-Ⅱ(Ang-Ⅱ)作用相关的胸主动脉瘤/夹层(Thoracic aortic aneurysms/dissection,TAAD)诊断标志物。方法利用数据集GSE35627筛选Ang-Ⅱ作用于大鼠原代主动脉平滑肌细胞的差异表达基因... 目的探讨基于转录组学及表观遗传组学筛选与血管紧张素-Ⅱ(Ang-Ⅱ)作用相关的胸主动脉瘤/夹层(Thoracic aortic aneurysms/dissection,TAAD)诊断标志物。方法利用数据集GSE35627筛选Ang-Ⅱ作用于大鼠原代主动脉平滑肌细胞的差异表达基因(DEGs)。取与Ang-Ⅱ相关数据集DEGs的交集,使用GeneMANIA数据库对交集基因进行功能分析和DO分析。使用R软件ChAMP包分析正常人和主动脉夹层(AD)患者升主动脉组织的表观基因组数据集GSE84274的差异甲基化水平,分析关键DEGs甲基化水平。使用人胸主动脉瘤(TAA)外周血样本全基因组基因表达谱数据集GSE9106 testing组绘制受试者工作特征曲线(ROC),预测诊断的有效性。结果在细胞水平、动物模型和TAAD临床病例的基础上,从GSE35627、GSE64613和GSE52093数据集得到6个交集基因:SLC7A8、WISP1、IL1R1、BCAT1、NID2及ATOH8。表观遗传组学分析发现,WISP1和NID2分别有5个和3个差异甲基化位点(DMPs)的甲基化水平存在明显差异。ROC曲线验证结果显示,WISP1的AUC为0.7,其特异度和敏感度较强,具有较高的准确性。结论通过转录组学及表观遗传组学分析可知,Ang-Ⅱ相关基因WISP1和NID2有助于TAAD的早期诊断。 展开更多
关键词 胸主动脉瘤/夹层 转录组学 表观遗传学 血管紧张素- 诊断标志物
下载PDF
妊娠高血压患者血管紧张素Ⅱ及AT1R、AT2R的表达及意义
5
作者 董在婷 熊琼英 《中国社区医师》 2024年第16期98-100,共3页
目的:探讨妊娠高血压(HDCP)患者血管紧张素Ⅱ(AngⅡ)及AngⅡ受体-1(AT1R)和AngⅡ受体-2(AT2R)的表达及意义。方法:选取2021年1月—2022月年9月孝感市中心医院收治的90例HDCP患者作为观察组,并将观察组根据病情程度分为HDCP组、轻度子痫... 目的:探讨妊娠高血压(HDCP)患者血管紧张素Ⅱ(AngⅡ)及AngⅡ受体-1(AT1R)和AngⅡ受体-2(AT2R)的表达及意义。方法:选取2021年1月—2022月年9月孝感市中心医院收治的90例HDCP患者作为观察组,并将观察组根据病情程度分为HDCP组、轻度子痫前期组和重度子痫前期组3个亚组,将同期产检的90例健康孕妇作为对照组。检测并比较观察组与对照组、观察组不同亚组AngⅡ水平、AT1R和AT2R阳性表达情况。结果:观察组产前母血、产后脐血AngⅡ水平低于对照组,产后母血AngⅡ水平、AT1R、AT2R总阳性率高于对照组,差异有统计学意义(P<0.05)。不同病情程度HDCP患者产前母血、产后脐血AngⅡ水平比较,HDCP组>轻度子痫前期组>重度子痫前期组;不同病情程度HDCP患者产后母血AngⅡ水平比较,HDCP组<轻度子痫前期组<重度子痫前期组;不同病情程度HDCP患者AT1R、AT2R阳性情况比较,HDCP组<轻度子痫前期组<重度子痫前期组,差异有统计学意义(P<0.05)。结论:HDCP患者母血、脐血AngⅡ存在异常表达,其AT1R、AT2R阳性率随病情加重而升高,检测上述指标有助于为HDCP发病机制、早期诊断与治疗提供参考。 展开更多
关键词 妊娠高血压 血管紧张素 血管紧张素受体-1 血管紧张素受体-2
下载PDF
羟基红花黄色素A抑制血管紧张素Ⅱ介导的心脏成纤维细胞肌化
6
作者 蒋洁 王立群 +3 位作者 刘雪茹 杨艳 谭晓秋 陈唐葶 《西南医科大学学报》 2024年第4期306-310,共5页
目的研究羟基红花黄色素A对血管紧张素Ⅱ介导的心脏成纤维细胞向肌成纤维细胞转化的影响。方法实验细胞随机分为正常对照组、Ang-Ⅱ组、Ang-Ⅱ+HSYA(5μM)组、Ang-Ⅱ+HSYA(25μM)组、Ang-Ⅱ+HSYA(50μM)组和Ang-Ⅱ+HSYA(100μM)组。使... 目的研究羟基红花黄色素A对血管紧张素Ⅱ介导的心脏成纤维细胞向肌成纤维细胞转化的影响。方法实验细胞随机分为正常对照组、Ang-Ⅱ组、Ang-Ⅱ+HSYA(5μM)组、Ang-Ⅱ+HSYA(25μM)组、Ang-Ⅱ+HSYA(50μM)组和Ang-Ⅱ+HSYA(100μM)组。使用划痕实验和Transwell小室侵袭实验检测细胞迁移侵袭情况,DCFH-DA荧光探针检测细胞内活性氧(reactive oxygenspecies,ROS)水平,Western blot检测α-SMA、CollagenⅠ、CollagenⅢ及转化生长因子β1(transforming growthfactor-β1,TGF-β1)的蛋白表达水平。结果Ang-Ⅱ促进心脏成纤维细胞迁移、增加ROS产生;而HSYA抑制了Ang-Ⅱ介导的心脏成纤维细胞迁移以及ROS产生;Western blot发现HSYA抑制了Ang-Ⅱ介导的心脏成纤维细胞α-SMA、CollagenⅠ、CollagenⅢ及TGF-β1的蛋白表达,差异具有统计学意义(P<0.05)。结论HSYA通过减少ROS的产生和下调TGF-β1信号通路抑制Ang-Ⅱ诱导心肌成纤维细胞向肌成纤维细胞分化。 展开更多
关键词 心肌纤维化 羟基红花黄色素A 血管紧张素 活性氧 转化生长因子Β1
下载PDF
Protective effects of icariin on human umbilical vein endothelial cell injured by angiotensin Ⅱ 被引量:3
7
作者 王秋娟 潘志伟 +3 位作者 王玉 杨涓 贾莹 孔令义 《Journal of Chinese Pharmaceutical Sciences》 CAS 2008年第1期16-21,共6页
To investigate the effects of icariin (ICA) on angiotensin Ⅱ(Ang Ⅱ)-induced injury in human umbilical vein endothelial cells line (ECV-304). The ECV-304 cells were cultured in vitro. After 24 h incubating with... To investigate the effects of icariin (ICA) on angiotensin Ⅱ(Ang Ⅱ)-induced injury in human umbilical vein endothelial cells line (ECV-304). The ECV-304 cells were cultured in vitro. After 24 h incubating with icariin, the model of AngⅡ-induced injury in ECV-304 was established. The cell viability (MTT method), Lactate dehydrogenase (LDH) release and Nitric oxide (NO) production in the medium, the capacity of scavenging superoxide anion radicals (O2^-) and hydroxyl radicals (.OH) were measured. The activities of superoxide dismutase (SOD), total nitric oxide synthase (T-NOS), inducible nitric oxide synthase (iNOS) and constitutive nitric oxide synthase (cNOS) in the cells were determined. Compared with the Ang Ⅱ-treated group, ICA can significantly raise the viability of EC, increase the activities of SOD, T-NOS and cNOS, increase the production of NO, enhance the capacity of scavenging superoxide anion radicals ( O2^- ) and hydroxyl radicals(.OH), and lower LDH leakage and iNOS activity. The results suggest that ICA can protect endothelial cells (ECV-304) from Ang II-induced injury. 展开更多
关键词 ICARIIN angiotensin Human umbilical vein endothelial cells line Nitric oxide
下载PDF
木犀草素抑制AngiotensinⅡ诱导的心肌细胞肥大 被引量:2
8
作者 杜小燕 侯颖 +2 位作者 覃华 韩艳 张琰 《科学技术与工程》 2010年第32期7890-7893,共4页
研究木犀草素对血管紧张素Ⅱ诱导乳鼠心肌细胞肥大的作用。用(1—3)d新生大鼠分离心肌细胞;用徕卡倒置显微镜抓获心肌细胞图像以测量细胞直径;用BCA蛋白检测法测定心肌细胞蛋白质含量;以[3H]苯丙氨酸掺入法测定心肌细胞的蛋白质合成率。... 研究木犀草素对血管紧张素Ⅱ诱导乳鼠心肌细胞肥大的作用。用(1—3)d新生大鼠分离心肌细胞;用徕卡倒置显微镜抓获心肌细胞图像以测量细胞直径;用BCA蛋白检测法测定心肌细胞蛋白质含量;以[3H]苯丙氨酸掺入法测定心肌细胞的蛋白质合成率。0.1μmol血管紧张素Ⅱ引起了心肌细胞直径、蛋白含量和心肌细胞蛋白质合成速率的显着增加。木犀草素预处理可剂量依赖性地减小由AngII刺激而引起的乳鼠心肌细胞直径、蛋白质含量和蛋白质的合成速率增加。结果表明,木犀草素可有效抑制血管紧张素Ⅱ诱导的心肌细胞肥大。 展开更多
关键词 血管紧张素 心肌细胞 肥大 木犀草素
下载PDF
急性心肌梗死患者血清IL-10、Ang-Ⅱ与冠脉病变严重程度的关系 被引量:2
9
作者 王宏娟 《河南医学研究》 CAS 2023年第6期1029-1033,共5页
目的分析血清白细胞介素(IL)-10、血管紧张素(Ang)-Ⅱ与急性心肌梗死(AMI)患者冠状动脉狭窄程度的关系。方法本研究为前瞻性研究,以2020年4月至2022年3月急诊入院拟行血管内介入诊治的120例AMI患者作为研究对象,根据Gensini冠状动脉评... 目的分析血清白细胞介素(IL)-10、血管紧张素(Ang)-Ⅱ与急性心肌梗死(AMI)患者冠状动脉狭窄程度的关系。方法本研究为前瞻性研究,以2020年4月至2022年3月急诊入院拟行血管内介入诊治的120例AMI患者作为研究对象,根据Gensini冠状动脉评分评估患者冠状动脉狭窄程度,分为轻度、中度、重度。在介入治疗前即对患者进行相关检查,检查项目包括血清IL-10、Ang-Ⅱ、肌钙蛋白I(cTnI),心肌酶[血清肌酸激酶(CK)、肌酸激酶同工酶(CK-MB)、乳酸脱氢酶(LDH)],血浆D-二聚体(D-D)等,分析血清IL-10、Ang-Ⅱ与AMI患者冠状动脉狭窄程度的关系及对冠状动脉狭窄程度的影响。结果研究中120例AMI患者,经剔除后以116例为研究对象,116例患者Gensini评分为22~76分,轻度狭窄28例,中度狭窄55例,重度狭窄33例。不同狭窄程度患者Killip分级,血清IL-10、Ang-Ⅱ、cTnI、CK、CK-MB、LDH,血浆D-D比较,差异有统计学意义(P<0.05);但不同狭窄程度患者Killip分级和血浆D-D两两比较,差异无统计学意义(P>0.05)。使用Kendall’s tau-b相关系数检验,血清IL-10、Ang-Ⅱ、cTnI、CK、CK-MB、LDH均与AMI患者冠状动脉狭窄程度呈正相关(r>0,P<0.05)。构建多元logistic回归模型提示,血清IL-10、Ang-Ⅱ高表达均能够加重AMI患者冠状动脉狭窄程度(P<0.05)。结论血清IL-10、Ang-Ⅱ均与AMI患者冠状动脉狭窄程度呈正相关,且能够促进冠状动脉狭窄进展,加重狭窄程度。 展开更多
关键词 急性心肌梗死 冠状动脉狭窄 白细胞介素-10 血管紧张素-
下载PDF
糖尿病合并颈动脉狭窄患者中AngⅡ调节T细胞活性的相关研究
10
作者 王凯 金凤 《内蒙古医科大学学报》 2023年第1期74-77,82,共5页
目的 本研究主要探究在2型糖尿病合并颈动脉狭窄患者中T细胞活性与AngⅡ水平的相关性。方法选择我院200例患者和30例健康体检者作为研究对象,分为CA组,100例2型糖尿病合并颈动脉狭窄患者;T2DM组,100例2型糖尿病患者;对照组,30例健康体... 目的 本研究主要探究在2型糖尿病合并颈动脉狭窄患者中T细胞活性与AngⅡ水平的相关性。方法选择我院200例患者和30例健康体检者作为研究对象,分为CA组,100例2型糖尿病合并颈动脉狭窄患者;T2DM组,100例2型糖尿病患者;对照组,30例健康体检者。收集病例资料并整理记录,检测T细胞比例,检测其转录因子T-bet、GATA3和RORγt以及其特征因子IFN-γ、IL-4和IL-17的变化;检测健康体检者外周血单核细胞中T细胞的变化。加用AngⅡ刺激剂和AngⅡ拮抗剂后观察T细胞的变化。结果 Th1和Th17阳性细胞数,其转录因子以及其表达因子在CA组明显高于T2DM组和对照组,差异有统计学意义(P <0.05)。健康体检者的外周血细胞在AngⅡ刺激剂作用下,效应性T细胞活性和T细胞的特征因子以及转录因子的表达明显增多,差异有统计学意义(P <0.05),而在AngⅡ拮抗剂作用下效应性T细胞活性和相关的表达因子表达明显减少,差异有统计学意义(P <0.05)。结论 通过调整T细胞活性和其表达因子,可能促进2型糖尿病的颈动脉狭窄形成和发展。AngⅡ可以调节T细活性及相关因子的表达,会导致合并高AngⅡ水平的T2DM患者发生颈动脉粥硬化。 展开更多
关键词 2型糖尿病 颈动脉粥样硬化 T细胞活性 angiotensin
下载PDF
PecsⅡ多模式镇痛在乳腺癌切除术中的应用效果及对术后疼痛-应激因子的影响
11
作者 杨广宇 祁富伟 +3 位作者 郑重 李珂 费凡 王敏 《临床误诊误治》 CAS 2023年第12期110-115,共6页
目的探究Ⅱ型胸神经阻滞(PecsⅡ)多模式镇痛在乳腺癌切除术中的应用效果及对术后疼痛-应激因子的影响。方法选取2020年4月—2022年3月行乳腺切除手术的成年女性乳腺癌90例,采用随机数字表法分为全身麻醉联合PecsⅡ多模式镇痛组(PGN组)... 目的探究Ⅱ型胸神经阻滞(PecsⅡ)多模式镇痛在乳腺癌切除术中的应用效果及对术后疼痛-应激因子的影响。方法选取2020年4月—2022年3月行乳腺切除手术的成年女性乳腺癌90例,采用随机数字表法分为全身麻醉联合PecsⅡ多模式镇痛组(PGN组)、全身麻醉联合PecsⅡ组(GN组)、单纯全身麻醉组(G组),每组30例。比较3组围术期指标、手术前后疼痛-应激因子[神经肽Y(NPY)、前列腺素E 2(PGE 2)、血管紧张素Ⅰ(AngⅠ)、皮质醇(Cor)]、疼痛程度[视觉模拟评分法(VAS)评分]、镇静程度(Ramsay评分)、生活质量[癌症患者生活质量量表(EORTC QLQ-C30)评分]、功能状态(KPS评分)及不良反应发生率;统计3组乳腺癌术后疼痛综合征(PMPS)发生情况。结果3组手术时间、术中出血量、麻醉时间比较差异无统计学意义(P>0.05);PGN组术后48 h阿片类药物用量少于GN组、G组,住院时间短于GN组、G组(P<0.01)。PGN组术后12、24、48 h血清NPY、PGE 2、AngⅠ、Cor水平低于GN组、G组(P<0.01);PGN组术后2、8、12、24、48 h VAS评分、Ramsay评分低于GN组、G组(P<0.01);PGN组术后3、6个月EORTC QLQ-C30评分、KPS评分高于GN组、G组(P<0.01)。术后48 h,3组不良反应发生率比较差异无统计学意义(P>0.05)。术后6个月,PGN组PMPS发生率低于GN组、G组(P<0.05)。结论PecsⅡ多模式镇痛能提高乳腺癌切除术后镇痛、镇静效果,有效抑制疼痛-应激因子,降低PMPS发生率,有助于改善患者生活质量。 展开更多
关键词 乳腺肿瘤 乳腺切除手术 型胸神经阻滞 神经肽Y 血管紧张素Ⅰ 皮质醇 镇静程度 疼痛综合征
下载PDF
内源性H_2S抑制angiotensin Ⅱ引起的神经元活性氧水平的升高 被引量:1
12
作者 曹冬青 刘小妮 +5 位作者 徐海艳 陶然 黄莺 金惠铭 王睿 卢宁 《中国病理生理杂志》 CAS CSCD 北大核心 2014年第5期837-841,共5页
目的:探讨内源性硫化氢(H2S)对血管紧张素Ⅱ(Ang Ⅱ)引起的延髓神经元活性氧(ROS)水平升高的作用及其可能机制。方法:首先培养原代延髓神经元;免疫荧光双标法鉴定神经元及内源性H2S生成酶胱硫醚β-合成酶(CBS)在神经元的表达;同时或单... 目的:探讨内源性硫化氢(H2S)对血管紧张素Ⅱ(Ang Ⅱ)引起的延髓神经元活性氧(ROS)水平升高的作用及其可能机制。方法:首先培养原代延髓神经元;免疫荧光双标法鉴定神经元及内源性H2S生成酶胱硫醚β-合成酶(CBS)在神经元的表达;同时或单独给予Ang Ⅱ(1μmol/L)和丁酸钠(NaBu,一种CBS激动剂;100μmol/L、250μmol/L和500μmol/L),二氢乙啶荧光探针法测定ROS水平;采用总超氧化物歧化酶(SOD)活性检测试剂盒观察总SOD的活性;real-time PCR观察CBS mRNA的表达。结果:(1)原代培养的90%以上的细胞为神经元,Ang Ⅱ(1μmol/L)升高延髓神经元ROS水平;(2)Ang Ⅱ抑制神经元总SOD的活性;(3)荧光双标显示CBS在延髓神经元有表达,Ang Ⅱ可降低CBS mRNA的表达;(4)NaBu(250μmol/L和500μmol/L)显著抑制Ang Ⅱ引起的ROS水平的升高,且呈剂量依赖性效应。而NaBu单独对延髓神经元ROS水平作用不明显。结论:Ang Ⅱ引起的延髓神经元ROS水平升高至少部分是通过降低总SOD的活性和CBS mRNA的表达而实现的;而内源性H2S可能通过相反的作用抑制这一过程。 展开更多
关键词 内源性硫化氢 神经元 血管紧张素 活性氧簇
下载PDF
AngiotensinⅡ通过氧化应激引起巨噬细胞AMPK/SIRT1能量信号紊乱 被引量:1
13
作者 肖珊 马郁文 +4 位作者 李婧 张彦红 何泓 方春香 王万铭 《南方医科大学学报》 CAS CSCD 北大核心 2021年第3期384-390,共7页
目的探讨Angiotensin Ⅱ诱导巨噬细胞氧化应激反应与AMPK/SIRT1通路活化关系的机制。方法 RAW264.7细胞正常培养后给予不同浓度的Angiotensin Ⅱ(0、0.5、1、3、10、20μmol/L)处理24 h后,Western blot检测AMPK,p-AMPK和SIRT1的表达水... 目的探讨Angiotensin Ⅱ诱导巨噬细胞氧化应激反应与AMPK/SIRT1通路活化关系的机制。方法 RAW264.7细胞正常培养后给予不同浓度的Angiotensin Ⅱ(0、0.5、1、3、10、20μmol/L)处理24 h后,Western blot检测AMPK,p-AMPK和SIRT1的表达水平变化,和用DCFH探针检测ROS水平的变化,试剂盒检测细胞上清液中SOD活性和MDA表达量;同时采用基因编辑技术将Angiotensin Ⅱ的受体AT1R成功沉默后给予Angiotensin Ⅱ刺激,检测对AMPK,p-AMPK和SIRT1蛋白水平的影响以及使用ROS的抑制剂来观察细胞AMPK和SIRT1的变化情况。结果 20μmol/L的Angiotensin Ⅱ的刺激能显著抑制蛋白AMPK的磷酸化(P<0.05),抑制SIRT1的表达;同时增加了细胞ROS的释放(P<0.05)。在检测SOD活性和MDA表达量时,0.5~10μmol/L的Angiotensin Ⅱ对细胞无明显改变(P>0.05),20μmol/L的Angiotensin Ⅱ明显抑制SOD活性(P<0.05),能显著增加MDA的产生。沉默了AT1R后,Angiotensin Ⅱ不能抑制AMPK蛋白磷酸化以及对SIRT1的表达无明显下调作用;使用ROS抑制剂后,Angiotensin Ⅱ处理无法降低细胞磷酸化AMPK和SIRT1的表达。结论 Angiotensin Ⅱ通过诱导巨噬细胞发生氧化应激反应从而引起AMPK/SIRT1信号通路的紊乱。 展开更多
关键词 血管紧张素2 AMPK/SIRT1 RAW264.7 氧化应激
下载PDF
Changes of c-fos and c-jun mRNA Expression in Angiotensin Ⅱ-induced Cardiomyocyte Hypertrophy and Effects of Sodium Tanshinone ⅡA Sulfonate 被引量:9
14
作者 周代星 梁黔生 +1 位作者 何雪心 占成业 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2008年第5期531-534,共4页
The changes of proto-oncogene c-fos and c-jun mRNA expression in angiotensin Ⅱ (AngⅡ)-induced hypertrophy and effects of sodium tanshinone ⅡA sulfonate (STS) in the primary culture of neonatal rat cardiomyocyte... The changes of proto-oncogene c-fos and c-jun mRNA expression in angiotensin Ⅱ (AngⅡ)-induced hypertrophy and effects of sodium tanshinone ⅡA sulfonate (STS) in the primary culture of neonatal rat cardiomyocytes were investigated. Twelve neonatal clean grade Wistar rats were selected. The cardiomyocytes were isolated, cultured and divided according to different treatments in the medium. The cardiomyocyte size was determined by phase contrast microscope, and the rate of protein synthesis was measured by [3H]-Leucine incorporation. The c-fos and c-jun mRNA expression in cardiomyocytes was detected by reverse transcription polymerase chain reaction (RT-PCR). It was found after cardiomyocytes were treated with AngⅡ for 30 min, the c-fos and c-jun mRNA expression in cardiomyocytes was increased significantly (P〈0.01). After treatment with AngⅡ for 24 h, the rate of protein synthesis in AngⅡ group was significantly increased as compared with control group (P〈0.01). After treatment with AngⅡ for 7 days, the size of cardiomyocytes in AngⅡ group was increased obviously as compared with control group (P〈0.05). After pretreatment with STS or Valsartan before AngⅡ treatment, both of them could inhibit the above effects of AngⅡ (P〈0.05 or P〈0.01). It was suggested that STS could ameliorate AngⅡ-induced cardiomyocyte hy- pertrophy by inhibiting c-fos and c-jun mRNA expression and reducing protein synthesis rate of cardiomyocytes. 展开更多
关键词 sodium tanshinone A sulfonate angiotensin cardiomyocyte hypertrophy C-LOS C-JUN
下载PDF
N-Glycosylated type Ⅱ collagen peptides as therapeutic saccharide vaccines for rheumatoid arthritis
15
作者 Dake Liu Shuyan Liu +2 位作者 Fanlei Hu Zhongtang Li Zhongjun Li 《Chinese Chemical Letters》 SCIE CAS CSCD 2024年第5期197-200,共4页
The interaction among type Ⅱ collagen(CⅡ),human DR4 major histocompatibility complex type Ⅱ molecule(MHC Ⅱ)and T-cell receptor(TCR)is associated with the development of rheumatoid arthritis(RA).The activation of T... The interaction among type Ⅱ collagen(CⅡ),human DR4 major histocompatibility complex type Ⅱ molecule(MHC Ⅱ)and T-cell receptor(TCR)is associated with the development of rheumatoid arthritis(RA).The activation of T cells can be reduced through exposure to modified CⅡ(263-272)glycopeptide fragment via competitive inhibition with self-antigen.In this work,30 peptides based on the sequence of CⅡ(263-272)were prepared and evaluated for their binding to DR4 protein by surface plasmon resonance(SPR)assay.The effect on the secretion of pro-inflammatory factors by the spleen cells in collagen induced rheumatoid arthritis(CIA)mouse was also investigated.Two N-glycosylated CⅡ peptides were identified to have strong binding to the human recombinant DR4 protein and weak proinflammatory effect.These glycopeptides could be developed as therapeutic saccharide vaccines for the treatment of rheumatoid arthritis(RA). 展开更多
关键词 Rheumatoid arthritis Typecollegan GLYCOSYLATION N-Glycosylated altered peptides Saccharide vaccines
原文传递
Association of Polymorphisms in Angiotensin-converting Enzyme and Type 1 Angiotensin Ⅱ Receptor Genes with Coronary Heart Disease and the Severity of Coronary Artery Stenosis 被引量:5
16
作者 邱春光 韩战营 +1 位作者 卢文杰 张存泰 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2007年第6期660-663,共4页
To explore the relation of angiotensin-converting enzyme (ACE) and angiotensin Ⅱ type 1 receptor (AT1R) gene polymorphism with coronary heart disease (CHD) and the severity of coronary artery stenosis, 130 CHD ... To explore the relation of angiotensin-converting enzyme (ACE) and angiotensin Ⅱ type 1 receptor (AT1R) gene polymorphism with coronary heart disease (CHD) and the severity of coronary artery stenosis, 130 CHD patients who underwent coronary angiography were examined for the number of affected coronary vessels (≥75% stenosis) and coronary Jeopardy score. The insertion/deletion of ACE gene polymorphism and AT1R gene polymorphism (an A→C transversion at nucleotide position 1166) were detected by using polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) in CHD patients and 90 healthy serving as controls. The resuits showed that DD genotype and of ACE were more frequent in CHD patients than that in control group (38.5% vs 14.4%, P〈0.001). The frequency of the ATIR A/C genotypes did not differ between the patients and the controls (10% vs 13.1%, P〉0.05). The relative risk associated with the ACE-DD was increased by AT1R-AC genotype. Neither the number of affected coronary vessels nor the coronary score differed among the ACE I/D genotypes (P〉0.05). But the number of affected coronary vessels and the coronary score were significantly greater in the patients with the AT1R-AC genotype than in those with the AA genotype (P〈0.05). In conclusion, DD genotype may be risk factor for CHD and MI in Chinese people, and is not responsible for the development of the coronary artery stenosis. The AT1R-C allele may increase the relative risk associated with the ACE-DD genotype, and may be involved in the development of the stenosis of coronary artery. 展开更多
关键词 angiotensin Ⅰ-converting enzyme angiotensin RECEPTOR gene polymorphism coronary angiography
下载PDF
Relationship between angiotensin-(1-7) and angiotensin Ⅱ correlates with hemodynamic changes in human liver cirrhosis 被引量:11
17
作者 Walkíria Wingester Vilas-Boas Antnio Ribeiro-Oliveira Jr +5 位作者 Regina Maria Pereira Renata da Cunha Ribeiro Jerusa Almeida Ana Paula Nadu Ana Cristina Simoes e Silva Robson Augusto Souza dos Santos 《World Journal of Gastroenterology》 SCIE CAS CSCD 2009年第20期2512-2519,共8页
AIM: To measure circulating angiotensins at different stages of human cirrhosis and to further evaluate a possible relationship between renin angiotensin system (RAS) components and hemodynamic changes. METHODS: P... AIM: To measure circulating angiotensins at different stages of human cirrhosis and to further evaluate a possible relationship between renin angiotensin system (RAS) components and hemodynamic changes. METHODS: Patients were allocated into 4 groups: mild-to-moderate liver disease (MLD), advanced liver disease (ALD), patients undergoing liver transplantation, and healthy controls. Blood was collected to determine plasma renin activity (PRA), angiotensin (Ang) Ⅰ, Ang Ⅱ, and Ang-(1-7) levels using radioimmunoassays. During liver transplantation, hemodynamic parameters were determined and blood was simultaneously obtained from the portal vein and radial artery in order to measure RAS components. RESULTS: PRA and angiotensins were elevated in ALD when compared to MLD and controls (P 〈 0.05). In contrast, Ang Ⅱ was significantly reduced in MLD. Ang-(1-7)/Ang Ⅱ ratios were increased in MLD when compared to controls and ALD. During transplantation, Ang Ⅱ levels were lower and Ang-(1-7)/Ang Ⅱ ratios were higher in the splanchnic circulation than in the peripheral circulation (0.52 ± 0.08 vs 0.38 ±0.04, P 〈 0.02), whereas the peripheral circulating Ang Ⅱ/Ang Ⅰ ratio was elevated in comparison to splanchnic levels (0.18 ±0.02 vs 0.13 ±0.02, P 〈 0.04). Ang-(1-7)/ Ang Ⅱ ratios positively correlated with cardiac output (r = 0.66) and negatively correlated with systemic vascular resistance (r = -0.70). CONCLUSION: Our findings suggest that the relationship between Ang-(1-7) and Ang Ⅱ may play a role in the hemodynamic changes of human cirrhosis. 展开更多
关键词 Renin-angiotensin system Liver cirrhosis angiotensin-(1-7) angiotensin Splanchnic circulation angiotensin converting enzyme 2
下载PDF
Construction of shRNA Targeted to the Rat Angiotensin Ⅱ Type 1 Receptors and Its RNAi in Cytoplasma 被引量:4
18
作者 肖传实 邱龄 曾秋棠 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2006年第1期4-8,共5页
The expression vector of shRNA targeted to the rat angiotensin Ⅱ receptor gene was constructed and the efficacy of siRNAs to modulate the expression of target gene in the in vitro cultured mammalian cells was investi... The expression vector of shRNA targeted to the rat angiotensin Ⅱ receptor gene was constructed and the efficacy of siRNAs to modulate the expression of target gene in the in vitro cultured mammalian cells was investigated for antihypertensive therapy in spontaneous hypertensive rat (SHR) at post-transcriptional level. The sense and antisense RNA oligonucleotides strands targeting angiotensin Ⅱ receptor mRNA were synthesized individually according to the sequence of the rat angiotensin Ⅱ receptor. For preparation of duplexes, sense- and antisense-stranded oligonucleotides were mixed and annealed, and the annealed duplexes were cloned into the pGenesil-1 vector. The rat glioma cells were transfected with constructed pGenesil-1-shRNA plasmid and scrambled plasmid. The cultured cells were collected at different phases. RT-PCR and Western blot were performed. The AT1 mRNA and protein levels behaved ultimately same. Compared to control after 48 h, AT1 mRNA levels were decreased to 35.5%±3.0 %, and the levels reached their lowest point after 72 h (20.7% ±4 % of control). At 24 and 48 h, AT1 protein was reduced to 46.9%±4.2% and 36.98%±3.7% respectively compared to control and a maximum reduction was observed after 72 h of incubation (28.1%± 4% compared to controls). Plasmid-based shRNA expression systems targeted against the rat angiotensin Ⅱ receptor gene were generated successfully. The shRNAs with a 22-nt stem and a short loop were cleaved into small interfering dsRNA (siRNA) by the Dicer. The in vitro transcribed siRNA enables the effective silencing of gene expression to the target mRNA and leads to effective inhibition of translation of proteins and will be lay the foundation of application of gene silencing technology to hypertensive rats. 展开更多
关键词 RNA interference HYPERTENSION angiotensin receptor vector
下载PDF
Expression of Angiotensin Ⅱ Receptors in Aldosterone-producing Adenoma of the Adrenal Gland and Their Clinical Significance 被引量:4
19
作者 吴准 倪栋 +7 位作者 闫永吉 李俊 王保军 欧阳金枝 张国玺 马鑫 李宏召 张旭 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2010年第4期486-489,共4页
The expression of angiotensin Ⅱ type 1 receptor (AT1R) and angiotensin Ⅱ type 2 receptor (AT2R) in aldosterone-producing adenoma (APA) of the adrenal gland was detected, and their relationship with clinical indexes ... The expression of angiotensin Ⅱ type 1 receptor (AT1R) and angiotensin Ⅱ type 2 receptor (AT2R) in aldosterone-producing adenoma (APA) of the adrenal gland was detected, and their relationship with clinical indexes of APA was analyzed. The mRNA expression of AT1R and AT2R in 50 cases of APA and tissues adjacent to tumors and 12 cases of normal adrenal tissues was detected by using reverse transcriptase polymerase chain reaction (RT-PCR). The expression of AT1R and AT2R proteins in paraffin-embedded slices of tissue was detected by immunohistochemistry. The expression of AT1R in adenoma, tissues adjacent to tumor, and normal tissues of the adrenal gland showed no significant differences. The expression of AT2R in APA tissue was lower than that in normal adrenal gland tissues (P<0.05). Correlation analysis of the mRNA expression level of AT2R and clinical data from patients demonstrated that AT2R expression was negatively related to plasma aldosterone concentration (PAC) (r=-0.467, P<0.05), but positively related with plasma renin activity (PRA) (r=0.604, P<0.05). It is concluded that down-regulation of the AT2R expression is possibly related with the tumorigenesis of APA. 展开更多
关键词 adrenal gland ALDOSTERONE ADENOMA angiotensin receptor
下载PDF
Valsartan Inhibits Angiotensin Ⅱ-induced Proliferation of Vascular Smooth Muscle Cells via Regulating the Expression of Mitofusin 2 被引量:4
20
作者 廖华 龚俊荣 +1 位作者 张文娟 郭小梅 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2012年第1期31-35,共5页
Angiotensin Ⅱ (ANGⅡ) plays an important role in the pathogenesis of atherosclerosis by inducing proliferation of vascular smooth muscle cells (VSMCs).In our study,we observed the effects of valsartan on proliferatio... Angiotensin Ⅱ (ANGⅡ) plays an important role in the pathogenesis of atherosclerosis by inducing proliferation of vascular smooth muscle cells (VSMCs).In our study,we observed the effects of valsartan on proliferation of cultured VSMCs treated with or without ANGⅡ by cell counting and methyl thiazolyl tetrazolium (MTT) assay,and detected the expression of mitofusin 2 (Mfn2),a newly discovered cell proliferation inhibitor and a related cell proliferation signaling pathway pro-tein by Western blotting.ANGⅡ at a concentration of 10-6 mol/L significantly stimulated VSMCs proliferation,down-regulated the expression of Mfn2 and upregulated the expression of Raf and ERK1/2.Valsartan inhibited such effects of ANGⅡ at concentrations of 10-5 and 10-6 mol/L,but not at 10-7 mol/L.Valsartan had no significant effect on the proliferation of untreated VSMCs.These results suggest that valsartan inhibits ANGⅡ-induced proliferation of VSMCs in vitro via Mfn2-Ras-Raf-ERK/MAPK signaling pathway. 展开更多
关键词 VALSARTAN angiotensin vascular smooth muscle cells PROLIFERATION mitofusin 2
下载PDF
上一页 1 2 171 下一页 到第
使用帮助 返回顶部