Objective:To study the effects of angiotensin converting enzyme inhibitor(ACEI)on cognitive function,apoptosis and oxidative stress in brain tissue of rats with cerebral infarction.Methods:Adult male SD rats were rand...Objective:To study the effects of angiotensin converting enzyme inhibitor(ACEI)on cognitive function,apoptosis and oxidative stress in brain tissue of rats with cerebral infarction.Methods:Adult male SD rats were randomly divided into control group,cerebral infarction group and ACEI group.The latter two groups were used to establish cerebral infarction model by thread embolism.The ACEI group was given oral administration of fosinopril 10mg/kg,and the other two groups were given oral administration of saline.The differences of Morris water maze cognitive function,apoptotic genes and oxidative stress indexes were compared among the three groups.Results:The escape latency of rats in cerebral infarction group was significantly longer than that in control group,the number of times of platform crossing was significantly less than that in control group,the duration of platform stay was significantly shorter than that in control group,the mRNA expression levels of Bcl-2 associated X protein(Bax),factor associated suicide(Fas),Fas ligand(FasL)and Caspase-3,the protein expression levels of phosphorylated Janus kinase(p-JAK2)and phosphorylated signal transducer and activator of transcription(p-STAT3)as well as the contents of reactive oxygen species(ROS)and malonaldehyde(MDA)in brain tissue were significantly higher than those in control group,and the mRNA expression level of B-cell lymphoma-2(Bcl-2)as well as the contents of catalase(CAT)and superoxide dismutase(SOD)in brain tissue was significantly lower than those in control group.The escape latency of rats in ACEI group was significantly shorter than that in cerebral infarction group,the number of times of platform crossing was significantly more than that in cerebral infarction group,the duration of platform stay was significantly longer than that in cerebral infarction group,the mRNA expression levels of Bax,Fas,FasL and Caspase-3,the protein expression levels of p-JAK2 and p-STAT3 as well as the contents of ROS and MDA in brain tissue were significantly lower than those in cerebral infarction group,and the mRNA expression level of Bcl-2 as well as the contents of CAT and SOD in brain tissue was significantly higher than those in cerebral infarction group.Conclusions:ACEI can improve the cognitive function of rats with cerebral infarction and inhibit the apoptosis and oxidative stress in ischemic brain tissue.展开更多
Objective: To observe the therapeutic effect of electroacupuncture (EA) on plasma angiotensin (Ang Ⅱ), aldosterone (ALD) and atrial natriuretic polypeptide (ANP) contents in experimental cerebral infarction rabbits f...Objective: To observe the therapeutic effect of electroacupuncture (EA) on plasma angiotensin (Ang Ⅱ), aldosterone (ALD) and atrial natriuretic polypeptide (ANP) contents in experimental cerebral infarction rabbits for analyzing the underlying mechanism of acupuncture in ameliorating blood supply of the brain tissue. Methods: A total of 80 rabbits were randomized into control (n = 8), pseudo-operation (n = 24), model (n = 24) and EA (n = 24) groups. Cerebral infarction model was established by infusion of self-thrombus into the carotid artery. EA (1 mA, 2 Hz) was applied to 'Baihui'(百会 GV 20) and 'Shuigou'(水沟 GV 26) for 30 min, once every 12 hours. Plasma Ang Ⅱ, ALD and ANP contents were detected with radioimmunoassay method. In the later 3 groups, blood samples were taken at 6 h, 24 h and 48 h after cerebral ischemia. Results: Compared with control and pseudo-operation groups, Ang Ⅱ and ALD contents of model group at 6 h, 24 h and 48 h after cerebral ischemia increased significantly while plasma ANP of the 3 time-courses of model group decreased considerably (P<0.01). In comparison with model group, results showed that Ang-Ⅱ and ALD contents of EA group decreased significantly whereas ANP level of EA group increased strikingly (P<0.01). Conclusion: Electroacupuncture has the effects of raising plasma ANP level and lowering plasma Ang-Ⅱ and ALD in cerebral infarction rabbits.展开更多
Objective:To investigate the effect of low-frequency rTMS combined with fasudil on neurological function in patients with acute cerebral infarction and its mechanism.Method:120 patients with acute cerebral infarction ...Objective:To investigate the effect of low-frequency rTMS combined with fasudil on neurological function in patients with acute cerebral infarction and its mechanism.Method:120 patients with acute cerebral infarction admitted from January 2016 to January 2019 were randomly divided into four groups:control group(n=30),fasudil group(n=30),rTMS group(n=30),fasudil+rTMS group(n=30).The changes of NIHSS score,ADL score,serum GAP-43,serum RhoA and serum ROCK-Ⅱwere observed and recorded before and after treatment.Results:The ADL scores and serum GAP-43 levels of the four groups after treatment were higher than those before treatment(P<0.05);the NIHSS scores,serum RhoA and serum ROCK-Ⅱlevels of the four groups after treatment were lower than those before treatment(P<0.05);After treatment,ADL score and serum GAP-43 level in fasudil+rTMS group were higher than those in other three groups(P<0.05);After treatment,NIHSS score,serum RhoA and serum ROCK-Ⅱlevels in fasudil+rTMS group were lower than those in other three groups(P<0.05).After treatment,NIHSS score was positively correlated with RhoA and ROCK-Ⅱcontent,negatively correlated with GAP-43 content and ADL score.Conclusions:Low-frequency rTMS combined with fasudil in the treatment of acute cerebral infarction can significantly improve neurological deficits,improve daily living ability,promote axonal remodeling and regeneration.It may be one of its mechanisms by increasing the expression of GAP-43 in serum and reducing the expression levels of RhoA and ROCK-Ⅱin serum.It has high clinical value.展开更多
Changes of hypothalamus angiotensin Ⅱ (A Ⅱ) and atrial natriuretic factor (ANF) levels and infarct volume were measured in hypertension and normotension rats at different time after focal cerebral ischemia. The resu...Changes of hypothalamus angiotensin Ⅱ (A Ⅱ) and atrial natriuretic factor (ANF) levels and infarct volume were measured in hypertension and normotension rats at different time after focal cerebral ischemia. The results showed that the hypothalamus ANF lev展开更多
基金Research Project of Yangpu District Science and Technology Commission,Yangpu District Health and Family Planning Commission.(No:YP18M15).
文摘Objective:To study the effects of angiotensin converting enzyme inhibitor(ACEI)on cognitive function,apoptosis and oxidative stress in brain tissue of rats with cerebral infarction.Methods:Adult male SD rats were randomly divided into control group,cerebral infarction group and ACEI group.The latter two groups were used to establish cerebral infarction model by thread embolism.The ACEI group was given oral administration of fosinopril 10mg/kg,and the other two groups were given oral administration of saline.The differences of Morris water maze cognitive function,apoptotic genes and oxidative stress indexes were compared among the three groups.Results:The escape latency of rats in cerebral infarction group was significantly longer than that in control group,the number of times of platform crossing was significantly less than that in control group,the duration of platform stay was significantly shorter than that in control group,the mRNA expression levels of Bcl-2 associated X protein(Bax),factor associated suicide(Fas),Fas ligand(FasL)and Caspase-3,the protein expression levels of phosphorylated Janus kinase(p-JAK2)and phosphorylated signal transducer and activator of transcription(p-STAT3)as well as the contents of reactive oxygen species(ROS)and malonaldehyde(MDA)in brain tissue were significantly higher than those in control group,and the mRNA expression level of B-cell lymphoma-2(Bcl-2)as well as the contents of catalase(CAT)and superoxide dismutase(SOD)in brain tissue was significantly lower than those in control group.The escape latency of rats in ACEI group was significantly shorter than that in cerebral infarction group,the number of times of platform crossing was significantly more than that in cerebral infarction group,the duration of platform stay was significantly longer than that in cerebral infarction group,the mRNA expression levels of Bax,Fas,FasL and Caspase-3,the protein expression levels of p-JAK2 and p-STAT3 as well as the contents of ROS and MDA in brain tissue were significantly lower than those in cerebral infarction group,and the mRNA expression level of Bcl-2 as well as the contents of CAT and SOD in brain tissue was significantly higher than those in cerebral infarction group.Conclusions:ACEI can improve the cognitive function of rats with cerebral infarction and inhibit the apoptosis and oxidative stress in ischemic brain tissue.
文摘Objective: To observe the therapeutic effect of electroacupuncture (EA) on plasma angiotensin (Ang Ⅱ), aldosterone (ALD) and atrial natriuretic polypeptide (ANP) contents in experimental cerebral infarction rabbits for analyzing the underlying mechanism of acupuncture in ameliorating blood supply of the brain tissue. Methods: A total of 80 rabbits were randomized into control (n = 8), pseudo-operation (n = 24), model (n = 24) and EA (n = 24) groups. Cerebral infarction model was established by infusion of self-thrombus into the carotid artery. EA (1 mA, 2 Hz) was applied to 'Baihui'(百会 GV 20) and 'Shuigou'(水沟 GV 26) for 30 min, once every 12 hours. Plasma Ang Ⅱ, ALD and ANP contents were detected with radioimmunoassay method. In the later 3 groups, blood samples were taken at 6 h, 24 h and 48 h after cerebral ischemia. Results: Compared with control and pseudo-operation groups, Ang Ⅱ and ALD contents of model group at 6 h, 24 h and 48 h after cerebral ischemia increased significantly while plasma ANP of the 3 time-courses of model group decreased considerably (P<0.01). In comparison with model group, results showed that Ang-Ⅱ and ALD contents of EA group decreased significantly whereas ANP level of EA group increased strikingly (P<0.01). Conclusion: Electroacupuncture has the effects of raising plasma ANP level and lowering plasma Ang-Ⅱ and ALD in cerebral infarction rabbits.
基金Scientific research and development plan project of North Sichuan Medical College(No.CBY13-A-QN17、CBY17-B-YB24).
文摘Objective:To investigate the effect of low-frequency rTMS combined with fasudil on neurological function in patients with acute cerebral infarction and its mechanism.Method:120 patients with acute cerebral infarction admitted from January 2016 to January 2019 were randomly divided into four groups:control group(n=30),fasudil group(n=30),rTMS group(n=30),fasudil+rTMS group(n=30).The changes of NIHSS score,ADL score,serum GAP-43,serum RhoA and serum ROCK-Ⅱwere observed and recorded before and after treatment.Results:The ADL scores and serum GAP-43 levels of the four groups after treatment were higher than those before treatment(P<0.05);the NIHSS scores,serum RhoA and serum ROCK-Ⅱlevels of the four groups after treatment were lower than those before treatment(P<0.05);After treatment,ADL score and serum GAP-43 level in fasudil+rTMS group were higher than those in other three groups(P<0.05);After treatment,NIHSS score,serum RhoA and serum ROCK-Ⅱlevels in fasudil+rTMS group were lower than those in other three groups(P<0.05).After treatment,NIHSS score was positively correlated with RhoA and ROCK-Ⅱcontent,negatively correlated with GAP-43 content and ADL score.Conclusions:Low-frequency rTMS combined with fasudil in the treatment of acute cerebral infarction can significantly improve neurological deficits,improve daily living ability,promote axonal remodeling and regeneration.It may be one of its mechanisms by increasing the expression of GAP-43 in serum and reducing the expression levels of RhoA and ROCK-Ⅱin serum.It has high clinical value.
文摘Changes of hypothalamus angiotensin Ⅱ (A Ⅱ) and atrial natriuretic factor (ANF) levels and infarct volume were measured in hypertension and normotension rats at different time after focal cerebral ischemia. The results showed that the hypothalamus ANF lev
