This study aimed to explore the effect of Bifidobacterium animalis F1-7 on the improvement of atherosclerotic inflammation.Arteriosclerosis model ApoE^(-/-)mice were orally administered with B.animalis F1-7 for 12 wee...This study aimed to explore the effect of Bifidobacterium animalis F1-7 on the improvement of atherosclerotic inflammation.Arteriosclerosis model ApoE^(-/-)mice were orally administered with B.animalis F1-7 for 12 weeks.The probiotic intervention reduced the plaque areas in aorta and the accumulation of macrophages,and downregulated the expression of toll-like receptor 4(TLR4)/nuclear factorκB(NF-κB)pathway to reduce the levels of inflammatory factors.The widely-targeted metabolomics analysis showed that acetyl-L-carnitine(ALC)in the intestine of atherosclerotic mice was significantly increased after B.animalis F1-7 intervention.Correlation analysis proved that ALC was associated with atherosclerotic inflammatory response.By using oxidized low density lipoprotein induced macrophage foam cells,we further verified that ALC could reduce lipid accumulation and inflammatory response in foam cells by downregulating the TLR4/NF-κB pathway.Finally,our results revealed that B.animalis F1-7 upregulated the metabolite ALC to downregulate the inflammatory responses,leading to the reduction of plaque accumulation of atherosclerosis.展开更多
The influence of relative humidity (RH) on quasistatic current-voltage (I-V) characteristics of Bifidobacterium animalis subsp. lactis BB-12 thin layers was studied for the first time. The value of electrical conducti...The influence of relative humidity (RH) on quasistatic current-voltage (I-V) characteristics of Bifidobacterium animalis subsp. lactis BB-12 thin layers was studied for the first time. The value of electrical conductivity in 75% RH was found to be in the order of 10-7 (ohm·cm)-1, which was 106 orders of magnitude higher than that observed in dry atmosphere. It was concluded that RH played a key role in hysteresis behavior of the measured (I-V) characteristics. FTIR measurements showed that under water moisture environment, the associated bonds between amine and carboxyl group were greatly strengthened that was the source of free charge carries after ionization. The surface charge of Bifidobacterium animalis subsp. lactis BB-12 was found to be negative by zeta potential measurements, claiming that electrons were the charge carriers.展开更多
With the stated advantages of finer adsorbent of high surface area, why then do manufacturers design unspecified active carbons of granular size even for the removal of medium size particles? This research attempts to...With the stated advantages of finer adsorbent of high surface area, why then do manufacturers design unspecified active carbons of granular size even for the removal of medium size particles? This research attempts to provide one of such answers. A batch equilibrium adsorption study was carried out to assess the adsorption capacity and intensities of methyl red dye onto “Received” Granular Activated Carbon (GAC) and “formulated” Powdered Activated Carbon (PAC) .The equilibrium data obtained were modeled using the Langmuir and Freundlich Isotherms. The data fitted best with the Langmuir model which was predicted by the highest R2 value (0.981). The experiment carried out demonstrated that GAC had a higher adsorption efficiency range of 33.4% to 93.55% and a high adsorption capacity of 1.176 mg/g. Generally, the research ascertained the reason why the manufacturer supplied the chosen adsorbent as “granulated particulate” instead of “powdered” as formulated for the purpose of this research.展开更多
A vegetable blend slurry consisting of aloe vera (Aloe barbadensis miller),garlic (Allium sativum),ginger (Zingiber officinale),liquorice (Glycyrrhiza glabra) and pumpkin seeds (Cucurbita pepo L.) was used as a probio...A vegetable blend slurry consisting of aloe vera (Aloe barbadensis miller),garlic (Allium sativum),ginger (Zingiber officinale),liquorice (Glycyrrhiza glabra) and pumpkin seeds (Cucurbita pepo L.) was used as a probiotic medium to evaluate the impact of probiotic fermentation on its chemical composition and flavour compound changes.Two commercial probiotic bacteria,Bifidobacterium animalis subsp.lactis HN019 and Lactobacillus acidophilus La-14,were used for monoculture and coculture fermentation over 72 h.After fermentation,sugars,organic acids and amino acids changed differently due to differences in metabolic pathways.In addition,there was a significant decrease in organosulfur compounds (from 10.56 ± 0.98 mg/L to 6.14 ± 0.83 mg/L,7.80 ± 1.09 mg/L and 6.02 ± 0.61 mg/L for B.lactis HN019 monoculture,L.acidophilus La-14 monoculture,and coculture fermentation,respectively) and total phenolic contents (TPC,from 24.61 ± 1.23 mg gallic acid equivalent (GAE)/g dry extract (DE) to 16.29 ± 1.22 and 15.66 ± 1.03 mg GAE/g DE for L.acidophilus La-14 monoculture and coculture fermentation,respectively).Interestingly,no significant changes in oxygen radical absorbance capacity (ORAC),total dietary fibre content and fat content after fermentation.Coculture fermentation did not show any significant synergistic or antagonistic effect.Our results suggest that the vegetable blend and the bacterial strains used have potential to create a novel probiotic product.展开更多
基金supported by Shandong Taishan industry leading talent project(LJNY202101)the National Key R&D of China(2018YFC0311201)。
文摘This study aimed to explore the effect of Bifidobacterium animalis F1-7 on the improvement of atherosclerotic inflammation.Arteriosclerosis model ApoE^(-/-)mice were orally administered with B.animalis F1-7 for 12 weeks.The probiotic intervention reduced the plaque areas in aorta and the accumulation of macrophages,and downregulated the expression of toll-like receptor 4(TLR4)/nuclear factorκB(NF-κB)pathway to reduce the levels of inflammatory factors.The widely-targeted metabolomics analysis showed that acetyl-L-carnitine(ALC)in the intestine of atherosclerotic mice was significantly increased after B.animalis F1-7 intervention.Correlation analysis proved that ALC was associated with atherosclerotic inflammatory response.By using oxidized low density lipoprotein induced macrophage foam cells,we further verified that ALC could reduce lipid accumulation and inflammatory response in foam cells by downregulating the TLR4/NF-κB pathway.Finally,our results revealed that B.animalis F1-7 upregulated the metabolite ALC to downregulate the inflammatory responses,leading to the reduction of plaque accumulation of atherosclerosis.
文摘The influence of relative humidity (RH) on quasistatic current-voltage (I-V) characteristics of Bifidobacterium animalis subsp. lactis BB-12 thin layers was studied for the first time. The value of electrical conductivity in 75% RH was found to be in the order of 10-7 (ohm·cm)-1, which was 106 orders of magnitude higher than that observed in dry atmosphere. It was concluded that RH played a key role in hysteresis behavior of the measured (I-V) characteristics. FTIR measurements showed that under water moisture environment, the associated bonds between amine and carboxyl group were greatly strengthened that was the source of free charge carries after ionization. The surface charge of Bifidobacterium animalis subsp. lactis BB-12 was found to be negative by zeta potential measurements, claiming that electrons were the charge carriers.
文摘With the stated advantages of finer adsorbent of high surface area, why then do manufacturers design unspecified active carbons of granular size even for the removal of medium size particles? This research attempts to provide one of such answers. A batch equilibrium adsorption study was carried out to assess the adsorption capacity and intensities of methyl red dye onto “Received” Granular Activated Carbon (GAC) and “formulated” Powdered Activated Carbon (PAC) .The equilibrium data obtained were modeled using the Langmuir and Freundlich Isotherms. The data fitted best with the Langmuir model which was predicted by the highest R2 value (0.981). The experiment carried out demonstrated that GAC had a higher adsorption efficiency range of 33.4% to 93.55% and a high adsorption capacity of 1.176 mg/g. Generally, the research ascertained the reason why the manufacturer supplied the chosen adsorbent as “granulated particulate” instead of “powdered” as formulated for the purpose of this research.
文摘A vegetable blend slurry consisting of aloe vera (Aloe barbadensis miller),garlic (Allium sativum),ginger (Zingiber officinale),liquorice (Glycyrrhiza glabra) and pumpkin seeds (Cucurbita pepo L.) was used as a probiotic medium to evaluate the impact of probiotic fermentation on its chemical composition and flavour compound changes.Two commercial probiotic bacteria,Bifidobacterium animalis subsp.lactis HN019 and Lactobacillus acidophilus La-14,were used for monoculture and coculture fermentation over 72 h.After fermentation,sugars,organic acids and amino acids changed differently due to differences in metabolic pathways.In addition,there was a significant decrease in organosulfur compounds (from 10.56 ± 0.98 mg/L to 6.14 ± 0.83 mg/L,7.80 ± 1.09 mg/L and 6.02 ± 0.61 mg/L for B.lactis HN019 monoculture,L.acidophilus La-14 monoculture,and coculture fermentation,respectively) and total phenolic contents (TPC,from 24.61 ± 1.23 mg gallic acid equivalent (GAE)/g dry extract (DE) to 16.29 ± 1.22 and 15.66 ± 1.03 mg GAE/g DE for L.acidophilus La-14 monoculture and coculture fermentation,respectively).Interestingly,no significant changes in oxygen radical absorbance capacity (ORAC),total dietary fibre content and fat content after fermentation.Coculture fermentation did not show any significant synergistic or antagonistic effect.Our results suggest that the vegetable blend and the bacterial strains used have potential to create a novel probiotic product.
