Work on the production and quality evaluation ofE. anomala spiced chocolate drinks was reported. This is done in order to establish the best concentration of the spice to be adopted in the chocolate drinks production....Work on the production and quality evaluation ofE. anomala spiced chocolate drinks was reported. This is done in order to establish the best concentration of the spice to be adopted in the chocolate drinks production. E. anomala seed powder was added into chocolate formulation in various concentrations [1%, 2%, 3%, 4%, as samples A, B, C, D with sample E as the control （no spice added）]. These samples were analyzed for difference quality attributes like： the proximate composition, minerals and chemical analysis, microbiological analysis, as well as the sensory evaluation using standard methods. The proximate composition showed the percentage moisture content ranging between 85.1 ±0.11 and 85.5± 0.10, ash content between 0.4 ± 0.00 and 0.5 ± 0.10, protein content between 1.0± 0.01 and 1.1 ± 0.11, fat content between 0.4 ± 0.1 land 0.5 ± 0.00 and carbohydrate content between 12.5 ±0.10 and 12.7 ±0.10. The minerals： 0.05 ±0.10 mg/kg, zinc： 0.01 4- 0.11 mg/kg, lead： 0.1 ± 0.10 mg/kg, and copper 0.1±0.1/l mg/kg, Mangeulese were obtained while cadmium was not detected at all. The chemical analysis gave between 5.03 ± 0. l t to 5.35 ±0.10, and pH 3.17 ± 0.10 to 3.67± 0.10, and total titratable acidity between 32.47 ± 0.10 to 32.50 ±0.10 tbr Brix. ttowever, sample C （3%） E. anomala spiced chocolate drink has the highest nutrients composition than all the other samples. All the samples have low microbial count, less than 3.5 ×10^3 load which is below the safe level of 1.0 ×10^3 but on the overall, sample C has the least value. Conclusively, smnple C, （chocolate spiced at 3% concentration） is established as the best sample nutritionally, microbiologically and on the overall acceptability.展开更多
野古草(Arundinella anomala var. depauperata Keng)在三峡库区长江及其支流江(河)岸有广泛分布,对水淹有很好的耐受能力。有研究表明许多植物在水淹时通气组织发生增强,通气组织的产生改善了植株通气状况,提高了植物对水淹的抵御能力...野古草(Arundinella anomala var. depauperata Keng)在三峡库区长江及其支流江(河)岸有广泛分布,对水淹有很好的耐受能力。有研究表明许多植物在水淹时通气组织发生增强,通气组织的产生改善了植株通气状况,提高了植物对水淹的抵御能力。为了研究水淹是否会影响野古草的通气组织发生以及野古草通气组织发生对水淹的反应,考察了不同水淹深度、不同水淹时间和不同水淹方式处理时野古草茎中通气组织的发生情况。实验中共设置3个水淹深度:不进行水淹(对照)、植株地下部分淹没、植株完全淹没于水下2m深处;5个淹没时间:植株被淹没的时间长度分别为5、10、20、30d和60d;2种水淹方式:连续水淹和间歇水淹。实验结果表明:(1)在无水淹情况下野古草茎中可以产生通气组织,通气组织产生随植株的生长而增强;水淹加快了野古草通气组织发生的进程,促进了野古草通气组织的提前发生。(2)野古草茎中通气组织并不会因为水淹的时间越长而产生越多,植株通气组织的大小达到一定程度后不再因水淹时间的增长而继续增大。(3)淹没深度对通气组织发生有一定影响,总的看来,地下部分淹没野古草植株的通气组织发生要强于完全淹没植株。(4)不同水淹方式对野古草通气组织发生的影响因水淹深度不同而有差异。在完全淹没情况下,连续水淹植株的通气组织比间歇水淹植株的通气组织发达;在地下部分淹没情况下,除水淹初期外,随水淹时间的延长,连续水淹植株通气组织发生与间歇水淹植株没有差异。展开更多
The scarab beetle, Anomala corpulenta Motschulsky（Coleoptera： Scarabaeoidea）, is a widespread and destructive pest in China. Vision is one of the most important means of acquiring information about the external env...The scarab beetle, Anomala corpulenta Motschulsky（Coleoptera： Scarabaeoidea）, is a widespread and destructive pest in China. Vision is one of the most important means of acquiring information about the external environment. In order to contribute to the understanding of the perception of visual stimuli in this species, the light sensitivity and spectral responses of the scarab beetle, A. corpulenta, were measured by using an electroretinogram（ERG） technique. In total, 14 monochromatic light intensities, between 340 and 605 nm, were applied to the compound eyes of A. corpulenta under varying levels of adaptation to dark and light conditions. The results showed that all light stimuli induced an ERG response, with varied amplitudes. The spectral sensitivity curve of dark-adapted eyes showed one major peak（~400 nm; near-ultraviolet）, a secondary peak（from 498 to 562 nm; yellow-green） and the third peakat 460 nm. By contrast, in light-adapted eyes, only a near-UV peak was observed. From these results, we conclude that the compound eye of A. corpulenta is likely to have at least three spectral types of photoreceptor. Significance of differences were also recorded in the responses of male and female compound eyes, as well as diurnally and nocturnally. The amplitude of ERG in response to white-light stimuli varied with the light intensity： The stronger the luminance, the higher the ERG value. This suggests that the compound eye of A. corpulenta adapts quickly to changing light conditions, enabling A. corpulenta to maintain nocturnal activities.展开更多
Anomala semicastanea species group consists of five species found in China: A. semicastanea Fairmaire, A. rufithorax Ohaus, A. shimenensis sp. nov., A. insulicola sp. nov. and A. atrovirens sp. nov.. Anomal...Anomala semicastanea species group consists of five species found in China: A. semicastanea Fairmaire, A. rufithorax Ohaus, A. shimenensis sp. nov., A. insulicola sp. nov. and A. atrovirens sp. nov.. Anomala amoena Frey, 1971 is designated as a syn. nov. to A. rufithorax Ohaus, 1933. All the type specimences are deposited in the Guangdong Entomological Institute.展开更多
We studied the roles of different concentrations of gibberellin A_(3)(GA_(3))in breaking hypocotyl dormancy of Paeonia anomala subsp.veitchii,aiming to provide a theoretical basis for the efficient elimination of seed...We studied the roles of different concentrations of gibberellin A_(3)(GA_(3))in breaking hypocotyl dormancy of Paeonia anomala subsp.veitchii,aiming to provide a theoretical basis for the efficient elimination of seed dormancy.The seeds of P.anomala subsp.veitchii were treated with different concentrations of GA_(3)(0,100,200,300,and 400 mg/L)under dark conditions at 16℃,and then the rooting indicators including rooting rate,rooting vigor,rooting index and mold rot rate were determined.The treatment with 400 mg/L GA_(3) for 4 h showed the best performance in breaking the hypocotyl dormancy.Specifically,the treatment shortened the rooting time to 12 d,increased the rooting rate,rooting vigor,and rooting index to 84.00%±5.29%,80.67%±5.03%,and 2.25±0.16 respectively,and decreased the mold rot rate to 0.67%±1.16%.To sum up,there were germination inhibitors in the seeds of P.anomala subsp.veitchii,while the addition of exogenous hormone GA_(3) can release the inhibitory effect on the hypocotyl,which indicated that GA_(3) can promote the breaking of hypocotyl dormancy.展开更多
Objective: To investigate the free radical scavenging, antidiabetic, kinetics and cytotoxic potentials of flavonoids extract of Dicoma anomala root by using standard methods. Methods:Antioxidant activity of the flavon...Objective: To investigate the free radical scavenging, antidiabetic, kinetics and cytotoxic potentials of flavonoids extract of Dicoma anomala root by using standard methods. Methods:Antioxidant activity of the flavonoids was investigated at scavenging free radicals such as 1,1-diphenyl-2-picrylhydrazyl, nitric oxide, hydroxyl radical, reducing capabilities, 2,2-azinobis(3-ethylbenzothiazoline-6) sulfonic acid as well as metal chelating capability at different concentrations(0.125-1.000 mg/mL) while the antidiabetic activity was evaluated via the inhibition and kinetics of carbohydrate digestive enzymes including, alpha glucosidase, sucrase and maltase. Brine shrimp lethality assay was also employed to examine the cytotoxic effects of the extract by using different range of concentrations(0.125-2.000 mg/mL). Results: The study revealed the best antioxidant activity of the extract in 1,1-diphenyl-2-picrylhydrazyl,2,2-azino-bis(3-ethylbenzothiazoline-6) sulfonic acid and nitric oxide having IC_(50) values of(386.90±4.91),(736.00±38.12),(629.30±9.62) μg/mL respectively compared with quercetin(standard) with IC_(50) [(522.20±12.38),(1 021.00±15.61) and(1 075.00±29.35) μg/mL]respective values while it was insignificantly(P>0.05) at par with quercetin for reducing power. Similarly, the extract exhibited a moderate inhibition of alpha glucosidase(43.1%),sucrase(33.4%) and maltase(29.9%) activities which were significantly(P<0.05) better than acarbose(18.4%, 12.7% and 24.9% respectively) although acarbose(46.1%) inhibited the higher activity of alpha amylase than the extract(13.7%). The kinetics of mode of inhibition of alpha amylase, alpha glucosidase, sucrase and maltase by flavonoids extract of Dicoma anomala revealed an uncompetitive, non-competitive, competitive and non-competitive inhibition respectively. The result of the lethality assay showed a potent cytotoxic effect of the flavonoids with LC_(50) value 0.510 mg/mL. Conclusions: The results obtained from this study are suggestive of the antioxidative, antidiabetic and cytotoxic potentials of flavonoids root extract of Dicoma anomala.展开更多
Hexavalent chromium （Cr（VI）） pollution has become one of the most serious environmental problems today. One removal strategy comprises the microbial reduction of Cr（VI）, is regarded as a cost-effective biotechno...Hexavalent chromium （Cr（VI）） pollution has become one of the most serious environmental problems today. One removal strategy comprises the microbial reduction of Cr（VI）, is regarded as a cost-effective biotechnology for the treatment of high volume and low concentration complex wastewaters. In this work a yeast strain that exhibits high Cr（VI） resistance was isolated from soil sediment. The isolated yeast was identified as Pichia anomala by sequencing analysis. The yeast showed a remarkable capacity to completely reduce 25 and 50 mg/L of Cr（VI） in 48 h under aerobic conditions. The increase of initial Cr（VI） concentration influenced reduction, growth and specific growth rate. This strain also exhibited multiple heavy metal tolerance. The presence of anions and cations in the medium had a great influence on chromium reduction. Fractionation of the cells showed that the mechanism of Cr（VI） removal by this strain is ＂adsorption-coupled reduction＂ and the hexavalent chromate reductase activity was expressed constitutively. FTIR analysis of the biomass exposed to chromium showed that the binding process of the chromium ions involves the active participation of functional groups present in the external surface of biomass. High Cr（VI） concentration resistance and high Cr（VI） reducing ability of this strain make it a suitable candidate for bioremediation.展开更多
文摘Work on the production and quality evaluation ofE. anomala spiced chocolate drinks was reported. This is done in order to establish the best concentration of the spice to be adopted in the chocolate drinks production. E. anomala seed powder was added into chocolate formulation in various concentrations [1%, 2%, 3%, 4%, as samples A, B, C, D with sample E as the control （no spice added）]. These samples were analyzed for difference quality attributes like： the proximate composition, minerals and chemical analysis, microbiological analysis, as well as the sensory evaluation using standard methods. The proximate composition showed the percentage moisture content ranging between 85.1 ±0.11 and 85.5± 0.10, ash content between 0.4 ± 0.00 and 0.5 ± 0.10, protein content between 1.0± 0.01 and 1.1 ± 0.11, fat content between 0.4 ± 0.1 land 0.5 ± 0.00 and carbohydrate content between 12.5 ±0.10 and 12.7 ±0.10. The minerals： 0.05 ±0.10 mg/kg, zinc： 0.01 4- 0.11 mg/kg, lead： 0.1 ± 0.10 mg/kg, and copper 0.1±0.1/l mg/kg, Mangeulese were obtained while cadmium was not detected at all. The chemical analysis gave between 5.03 ± 0. l t to 5.35 ±0.10, and pH 3.17 ± 0.10 to 3.67± 0.10, and total titratable acidity between 32.47 ± 0.10 to 32.50 ±0.10 tbr Brix. ttowever, sample C （3%） E. anomala spiced chocolate drink has the highest nutrients composition than all the other samples. All the samples have low microbial count, less than 3.5 ×10^3 load which is below the safe level of 1.0 ×10^3 but on the overall, sample C has the least value. Conclusively, smnple C, （chocolate spiced at 3% concentration） is established as the best sample nutritionally, microbiologically and on the overall acceptability.
文摘野古草(Arundinella anomala var. depauperata Keng)在三峡库区长江及其支流江(河)岸有广泛分布,对水淹有很好的耐受能力。有研究表明许多植物在水淹时通气组织发生增强,通气组织的产生改善了植株通气状况,提高了植物对水淹的抵御能力。为了研究水淹是否会影响野古草的通气组织发生以及野古草通气组织发生对水淹的反应,考察了不同水淹深度、不同水淹时间和不同水淹方式处理时野古草茎中通气组织的发生情况。实验中共设置3个水淹深度:不进行水淹(对照)、植株地下部分淹没、植株完全淹没于水下2m深处;5个淹没时间:植株被淹没的时间长度分别为5、10、20、30d和60d;2种水淹方式:连续水淹和间歇水淹。实验结果表明:(1)在无水淹情况下野古草茎中可以产生通气组织,通气组织产生随植株的生长而增强;水淹加快了野古草通气组织发生的进程,促进了野古草通气组织的提前发生。(2)野古草茎中通气组织并不会因为水淹的时间越长而产生越多,植株通气组织的大小达到一定程度后不再因水淹时间的增长而继续增大。(3)淹没深度对通气组织发生有一定影响,总的看来,地下部分淹没野古草植株的通气组织发生要强于完全淹没植株。(4)不同水淹方式对野古草通气组织发生的影响因水淹深度不同而有差异。在完全淹没情况下,连续水淹植株的通气组织比间歇水淹植株的通气组织发达;在地下部分淹没情况下,除水淹初期外,随水淹时间的延长,连续水淹植株通气组织发生与间歇水淹植株没有差异。
基金supported by the China Agricultural Research Stem (CARS-03)the Special Fund for Agro-Scientific Research in Public Interest,China (201003025)
文摘The scarab beetle, Anomala corpulenta Motschulsky（Coleoptera： Scarabaeoidea）, is a widespread and destructive pest in China. Vision is one of the most important means of acquiring information about the external environment. In order to contribute to the understanding of the perception of visual stimuli in this species, the light sensitivity and spectral responses of the scarab beetle, A. corpulenta, were measured by using an electroretinogram（ERG） technique. In total, 14 monochromatic light intensities, between 340 and 605 nm, were applied to the compound eyes of A. corpulenta under varying levels of adaptation to dark and light conditions. The results showed that all light stimuli induced an ERG response, with varied amplitudes. The spectral sensitivity curve of dark-adapted eyes showed one major peak（~400 nm; near-ultraviolet）, a secondary peak（from 498 to 562 nm; yellow-green） and the third peakat 460 nm. By contrast, in light-adapted eyes, only a near-UV peak was observed. From these results, we conclude that the compound eye of A. corpulenta is likely to have at least three spectral types of photoreceptor. Significance of differences were also recorded in the responses of male and female compound eyes, as well as diurnally and nocturnally. The amplitude of ERG in response to white-light stimuli varied with the light intensity： The stronger the luminance, the higher the ERG value. This suggests that the compound eye of A. corpulenta adapts quickly to changing light conditions, enabling A. corpulenta to maintain nocturnal activities.
文摘Anomala semicastanea species group consists of five species found in China: A. semicastanea Fairmaire, A. rufithorax Ohaus, A. shimenensis sp. nov., A. insulicola sp. nov. and A. atrovirens sp. nov.. Anomala amoena Frey, 1971 is designated as a syn. nov. to A. rufithorax Ohaus, 1933. All the type specimences are deposited in the Guangdong Entomological Institute.
文摘We studied the roles of different concentrations of gibberellin A_(3)(GA_(3))in breaking hypocotyl dormancy of Paeonia anomala subsp.veitchii,aiming to provide a theoretical basis for the efficient elimination of seed dormancy.The seeds of P.anomala subsp.veitchii were treated with different concentrations of GA_(3)(0,100,200,300,and 400 mg/L)under dark conditions at 16℃,and then the rooting indicators including rooting rate,rooting vigor,rooting index and mold rot rate were determined.The treatment with 400 mg/L GA_(3) for 4 h showed the best performance in breaking the hypocotyl dormancy.Specifically,the treatment shortened the rooting time to 12 d,increased the rooting rate,rooting vigor,and rooting index to 84.00%±5.29%,80.67%±5.03%,and 2.25±0.16 respectively,and decreased the mold rot rate to 0.67%±1.16%.To sum up,there were germination inhibitors in the seeds of P.anomala subsp.veitchii,while the addition of exogenous hormone GA_(3) can release the inhibitory effect on the hypocotyl,which indicated that GA_(3) can promote the breaking of hypocotyl dormancy.
基金supported by the Postdoctoral Research Fellowship(2-459-B3425)of Directorate Research and Development,University of Free State,South Africa
文摘Objective: To investigate the free radical scavenging, antidiabetic, kinetics and cytotoxic potentials of flavonoids extract of Dicoma anomala root by using standard methods. Methods:Antioxidant activity of the flavonoids was investigated at scavenging free radicals such as 1,1-diphenyl-2-picrylhydrazyl, nitric oxide, hydroxyl radical, reducing capabilities, 2,2-azinobis(3-ethylbenzothiazoline-6) sulfonic acid as well as metal chelating capability at different concentrations(0.125-1.000 mg/mL) while the antidiabetic activity was evaluated via the inhibition and kinetics of carbohydrate digestive enzymes including, alpha glucosidase, sucrase and maltase. Brine shrimp lethality assay was also employed to examine the cytotoxic effects of the extract by using different range of concentrations(0.125-2.000 mg/mL). Results: The study revealed the best antioxidant activity of the extract in 1,1-diphenyl-2-picrylhydrazyl,2,2-azino-bis(3-ethylbenzothiazoline-6) sulfonic acid and nitric oxide having IC_(50) values of(386.90±4.91),(736.00±38.12),(629.30±9.62) μg/mL respectively compared with quercetin(standard) with IC_(50) [(522.20±12.38),(1 021.00±15.61) and(1 075.00±29.35) μg/mL]respective values while it was insignificantly(P>0.05) at par with quercetin for reducing power. Similarly, the extract exhibited a moderate inhibition of alpha glucosidase(43.1%),sucrase(33.4%) and maltase(29.9%) activities which were significantly(P<0.05) better than acarbose(18.4%, 12.7% and 24.9% respectively) although acarbose(46.1%) inhibited the higher activity of alpha amylase than the extract(13.7%). The kinetics of mode of inhibition of alpha amylase, alpha glucosidase, sucrase and maltase by flavonoids extract of Dicoma anomala revealed an uncompetitive, non-competitive, competitive and non-competitive inhibition respectively. The result of the lethality assay showed a potent cytotoxic effect of the flavonoids with LC_(50) value 0.510 mg/mL. Conclusions: The results obtained from this study are suggestive of the antioxidative, antidiabetic and cytotoxic potentials of flavonoids root extract of Dicoma anomala.
文摘Hexavalent chromium （Cr（VI）） pollution has become one of the most serious environmental problems today. One removal strategy comprises the microbial reduction of Cr（VI）, is regarded as a cost-effective biotechnology for the treatment of high volume and low concentration complex wastewaters. In this work a yeast strain that exhibits high Cr（VI） resistance was isolated from soil sediment. The isolated yeast was identified as Pichia anomala by sequencing analysis. The yeast showed a remarkable capacity to completely reduce 25 and 50 mg/L of Cr（VI） in 48 h under aerobic conditions. The increase of initial Cr（VI） concentration influenced reduction, growth and specific growth rate. This strain also exhibited multiple heavy metal tolerance. The presence of anions and cations in the medium had a great influence on chromium reduction. Fractionation of the cells showed that the mechanism of Cr（VI） removal by this strain is ＂adsorption-coupled reduction＂ and the hexavalent chromate reductase activity was expressed constitutively. FTIR analysis of the biomass exposed to chromium showed that the binding process of the chromium ions involves the active participation of functional groups present in the external surface of biomass. High Cr（VI） concentration resistance and high Cr（VI） reducing ability of this strain make it a suitable candidate for bioremediation.
