High efficient removal of antibiotics during nutriments recovery for biomass production poses a major technical challenge for photosynthetic microbial biofilm-based wastewater treatment since antibiotics are always co...High efficient removal of antibiotics during nutriments recovery for biomass production poses a major technical challenge for photosynthetic microbial biofilm-based wastewater treatment since antibiotics are always co-exist with nutriments in wastewater and resist biodegradation due to their strong biotoxicity and recalcitrance.In this study,we make a first attempt to enhance metronidazole(MNZ)removal from wastewater using electrochemistry-activated binary-species photosynthetic biofilm of Rhodopseudomonas Palustris(R.Palustris)and Chlorella vulgaris(C.vulgaris)by cultivating them under different applied potentials.The results showed that application of external potentials of-0.3,0 and 0.2 V led to 11,33 and 26-fold acceleration in MNZ removal,respectively,as compared to that of potential free.The extent of enhancement in MNZ removal was positively correlated to the intensities of photosynthetic current produced under different externally applied potentials.The binary-species photoelectrogenic biofilm exhibited 18 and 6-fold higher MNZ removal rate than that of single-species of C.vulgaris and R.Palustris,respectively,due to the enhanced metabolic interaction between them.Application of an external potential of 0V significantly promoted the accumulation of tryptophan and tyrosine-like compounds as well as humic acid in ex-tracellular polymeric substance,whose concentrations were 7.4,7.1 and 2.0-fold higher than those produced at potential free,contributing to accelerated adsorption and reductive and photosensitive degradation of MNZ.展开更多
【目的】菌株MIM37为具有两种光能利用途径的光合异养细菌,分析其基因组和光照对生长的影响,为理解光能利用途径、光营养生物多样性以及光合作用的进化和功能等提供线索。【方法】采用平板涂布划线法分离菌株,结合形态观察及16S r RNA...【目的】菌株MIM37为具有两种光能利用途径的光合异养细菌,分析其基因组和光照对生长的影响,为理解光能利用途径、光营养生物多样性以及光合作用的进化和功能等提供线索。【方法】采用平板涂布划线法分离菌株,结合形态观察及16S r RNA基因和光合基因序列同源性与系统发育分析进行初步分类鉴定;以分光光度法和荧光显微观察法测定光照和黑暗培养下培养液细胞浓度和单细胞体积;构建片段长度为300-500 bp的Illumina PE文库,以Illumina Hiseq2000进行基因组测序,以SOAPdenovo和Gap Closer组装序列,以RAST在线软件注释基因组。【结果】从内蒙古腾格里沙漠天鹅湖表层水中分离获得一株细菌MIM37,经16S r RNA基因、puf M和视紫质基因同源性和系统发育分析均显示其与Sphingomonas属亲缘关系最为密切;相对黑暗培养,光照刺激下的最大细胞浓度和单细胞体积大小分别提高了1.2和5.6倍;基因组注释显示MIM37代谢途径多样,含典型好氧菌的呼吸电子传递链,具有完整的好氧不产氧细菌的光合基因簇及Xanthorhodopsin-like视紫质蛋白基因,合成铁载体,还原重金属,降解微囊藻毒素和多环芳烃类等。【结论】MIM37属于Sphingomonas属,具有两种光能利用途径,光照可明显促进其生长,多样的代谢模式可能使其在自然环境中极具竞争力、分布广泛并具有应用于修复环境污染的潜力。展开更多
基金The authors thank the financial support provided by the National Natural Science Foundation of China(No.51108186)the Tip-top Scientific and Technical Innovative Youth Talents of Guangdong Special Support Program,China(No.2015TQ01Z039)the Natural Science Research Project of Higher Education Institutions of Guangdong Province(No.2015KTSCX025).
文摘High efficient removal of antibiotics during nutriments recovery for biomass production poses a major technical challenge for photosynthetic microbial biofilm-based wastewater treatment since antibiotics are always co-exist with nutriments in wastewater and resist biodegradation due to their strong biotoxicity and recalcitrance.In this study,we make a first attempt to enhance metronidazole(MNZ)removal from wastewater using electrochemistry-activated binary-species photosynthetic biofilm of Rhodopseudomonas Palustris(R.Palustris)and Chlorella vulgaris(C.vulgaris)by cultivating them under different applied potentials.The results showed that application of external potentials of-0.3,0 and 0.2 V led to 11,33 and 26-fold acceleration in MNZ removal,respectively,as compared to that of potential free.The extent of enhancement in MNZ removal was positively correlated to the intensities of photosynthetic current produced under different externally applied potentials.The binary-species photoelectrogenic biofilm exhibited 18 and 6-fold higher MNZ removal rate than that of single-species of C.vulgaris and R.Palustris,respectively,due to the enhanced metabolic interaction between them.Application of an external potential of 0V significantly promoted the accumulation of tryptophan and tyrosine-like compounds as well as humic acid in ex-tracellular polymeric substance,whose concentrations were 7.4,7.1 and 2.0-fold higher than those produced at potential free,contributing to accelerated adsorption and reductive and photosensitive degradation of MNZ.
文摘【目的】菌株MIM37为具有两种光能利用途径的光合异养细菌,分析其基因组和光照对生长的影响,为理解光能利用途径、光营养生物多样性以及光合作用的进化和功能等提供线索。【方法】采用平板涂布划线法分离菌株,结合形态观察及16S r RNA基因和光合基因序列同源性与系统发育分析进行初步分类鉴定;以分光光度法和荧光显微观察法测定光照和黑暗培养下培养液细胞浓度和单细胞体积;构建片段长度为300-500 bp的Illumina PE文库,以Illumina Hiseq2000进行基因组测序,以SOAPdenovo和Gap Closer组装序列,以RAST在线软件注释基因组。【结果】从内蒙古腾格里沙漠天鹅湖表层水中分离获得一株细菌MIM37,经16S r RNA基因、puf M和视紫质基因同源性和系统发育分析均显示其与Sphingomonas属亲缘关系最为密切;相对黑暗培养,光照刺激下的最大细胞浓度和单细胞体积大小分别提高了1.2和5.6倍;基因组注释显示MIM37代谢途径多样,含典型好氧菌的呼吸电子传递链,具有完整的好氧不产氧细菌的光合基因簇及Xanthorhodopsin-like视紫质蛋白基因,合成铁载体,还原重金属,降解微囊藻毒素和多环芳烃类等。【结论】MIM37属于Sphingomonas属,具有两种光能利用途径,光照可明显促进其生长,多样的代谢模式可能使其在自然环境中极具竞争力、分布广泛并具有应用于修复环境污染的潜力。
