基于3,5-二溴水杨醛席夫碱镍配合物-氧化石墨烯电化学免疫传感器检测Anti-IgG含量的研究

应用物理吸附法将羊抗人IgG抗原直接固定于3,5-二溴水杨醛席夫碱镍配合物-氧化石墨烯修饰的金电极表面,制备电化学免疫传感器。采用循环伏安法和交流阻抗法对传感器进行表征,结果表明该传感器适合检测Anti-IgG浓度。同时探讨了缓冲液pH值、扫描速度、免疫反应温度、抗原与抗体配比对循环伏安峰电流的影响,结果表明在5-100mV/s扫速范围内,峰电流与扫速呈线性。孵育最优条件为25℃,h-IgG与Anti-IgG配比为1∶1。循环伏安法研究还表明Anti-IgG浓度在0.01-260μg/L范围内,线性关系良好,相关系数r^2=0.993,检出限（S/N=3）为0.006μg/L,据此建立了检测Anti-IgG浓度的新方法。

信号分子IRAK1/4在antiβ-2GPI/β2GPI复合物诱导THP-1细胞表达TF中的作用探讨

目的:探讨IRAK1和IRAK4在antiβ-2GPI/β2GPI复合物诱导单核细胞株THP-1表达组织因子(TF)中的作用。方法:利用荧光定量PCR(Real-time PCR)、TF活性试剂盒分别检测THP-1细胞表达TF mRNA及TF活性;Western blot检测anti-β2GPI/β2GPI复合物诱导THP-1细胞表达IRAK1、磷酸化-IRAK1(p-IRAK1)、IRAK4情况;观察IRAK1/4抑制物是否干预anti-β2GPI/β2GPI复合物诱导THP-1表达TF。结果:Antiβ-2GPI/β2GPI复合物(100μg/ml)诱导THP-1细胞表达TF显著增加(P<0.05 vs control);Antiβ-2GPI/β2GPI复合物(100μg/ml)刺激THP-1细胞表达IRAK1、p-IRAK1、IRAK4(蛋白)显著升高(P<0.05 vscontrol);IRAK1/4抑制物(50μmol/L)能够阻断antiβ-2GPI/β2GPI复合物(100μg/ml)诱导THP-1表达TF及IRAK1磷酸化的效应。结论:antiβ-2GPI/β2GPI复合物诱导THP-1细胞表达TF过程中,信号分子IRAK1/4被激活进而发挥重要作用。

三维Anti de Sitter空间中Lorentzian曲面的S_t^1×S_s^1-值光锥Gauss映射的奇点分类

利用Arnol'd的Legendrian理论,对三维Anti de Sitter空间中Lorentzian曲面进行了研究.引入光维高度函数概念研究了三维Anti de Sitter空间Lorentzian曲面的S1t×S1s-值、光锥Gauss映射的奇点,进行了奇点分类,揭示了类光Causs-kronecker曲率之间的关系;并研究了Lorentzian曲面的一些基本几何性质.

On the Construction of Deepening Anti - corruption Mechanism

It needs the foundation of system and the guarantee of organizational system for anti-corruption,but it is more necessary to build and form an effective anti-corruption mechanism,so that the anti-corruption can be really put into practice. Anti-corruption mechanism refers to a organic operation system of the interaction,interconnection and constraint between the constituent elements( parts) and elements of national anti-corruption,and as a system,anti-corruption mechanism should have the characteristics of system aticness,comprehensiveness,transparency,legalization,public participation,scientific dynam ic,and internationalism. The construction of deepening anti-corruption mechanism is the need for reconstructing the ruling legitimacy of the party and the governm ent. Adhering to the principle of treating both root causes and symptoms is necessary in the construction of anti-corruption m echanism,com bating and punishing corruption is an important part of anti-corruption,and the prevention and control of corruption is the basic project of anti-corruption. Therefore,the construction of prevention and control mechanism in the anti-corruption mechanism has a more far-reaching significance.

Highly Efficient Rinse-Off/Leave-On Scalp Care Treatments to Reduce Moderate to Severe Dandruff

Background/Aim: Dandruff is a common scalp problem associated with flaky and inflamed skin. In this study, we investigated the efficacy of a shampoo containing piroctone olamine and climbazole and the combination of this shampoo with a scalp tonic containing piroctone olamine and licochalcone A, derived from Glycyrrhiza inflata root extract, to reduce dandruff. Study Design/Methods: After conditioning, 102 subjects (♂ 56 and 46 ♀) with moderate to very strong dandruff affection underwent a randomized half head study for 4 weeks. The volunteers’ heads were washed regularly either with a placebo or the anti-dandruff shampoo, followed by the anti-dandruff tonic application or no treatment. In a 2-week post-treatment phase, subjects only applied placebo shampoo. Every two weeks, trained experts assessed dandruff affection based on a scale of 1 to 6. At study start and after every 2 weeks, cytokine concentrations and anti-fungal activity of test products were determined in scalp wash-ups by enzyme-linked immunosorbant assays or a Malassezia colony-forming assay, respectively. Results: Results of expert grading and anti-fungal activity revealed a significant reduction in dandruff affection and Malassezia colony-forming units after treatment with the anti-dandruff shampoo or its combination with the tonic. Dandruff affection even remained reduced in the post-treatment phase when levels of Malassezia colony-forming units had returned to baseline. Cytokine analyses proved a significant decrease in pro-inflammatory dandruff markers after treatment with both anti-dandruff products. For the shampoo/tonic combination, a superior reduction of one scalp inflammatory marker was determined even after the post-treatment phase. Conclusion: Both the rinse-off shampoo as well as its combination with the leave-on tonic excellently alleviated dandruff and its associated micro-inflammation. Both treatments showed anti-fungal activity. The superior benefit, exerted by the combination, is primarily based on the known anti-inflammatory effect of licochalcone A and the improved scalp substantivity of the leave-on application.

高效液相色谱法测定洗发护发产品中18种防腐剂和去屑剂

建立高效液相色谱法(HPLC)同时测定不同基质化妆品中18种防腐剂和去屑剂组分的含量,并对50批市售洗发护发类化妆品进行质量分析。不同基质样品经前处理提取后,采用HPLC法分析,外标法定量。选取C18色谱柱(4.6 mm×250 mm,5μm),甲醇和磷酸二氢钠水溶液(pH调节至3.8)作为流动相,进行梯度洗脱,流速为1.0 mL/min,柱温30℃,选取二极管阵列检测器多波长分析。18种组分在色谱图中分离度良好,峰面积与质量浓度在0.4~600μg/mL范围内呈良好的线性关系(r>0.9983),检出浓度在0.4~10 mg/kg之间。多种组分平均回收率(n=6)为83.1%~110.4%,RSD为0.12%~7.6%,回收率良好;多种组分在24 h内保持稳定,RSD在0.15%~3.8%之间,稳定性良好。50批样品中检出9种成分,吡硫鎓锌、吡罗克酮乙醇胺盐、对氯间甲酚、氯咪巴唑、三氯生、水杨酸的含量分别为2100~6800,39~2900,130~370,24~1600,0.14~0.19,28~1900 mg/kg。苄氯酚、邻氨基苯酚、邻伞花烃-5-醇的含量检出但是含量小于定量限,无法准确定量。该方法经方法学验证,可同时测定化妆品中18种防腐剂和去屑剂组分的含量。

Anti-Bacterial and Flame Retardant for Cotton Fabric by One-Bath Finishing

A research on the process of cotton fabric flame-re-tarding,anti-bacterial finishing and one-bath finish-ing of anti-bacterial and flame-retarding is discussed.The flame retardant agent was phosphorous-contained,and the bacteriostatic finishing agent named SFR-1 wassynthesized.The flame retardancy of the fabric finishedcan meet the DOC FF3-71 Children Sleepwear Stan-dard.Its bacterial inhibiting capacity can meet and ex-ceed the requirements of similar products

Testing of Anti-stripping Property of Friction Spun Core Yarn

Friction spun core yarn has two components: filament core and staple fiber sheath. Under axial rubbing action, the failure mode of the core yarn is the stripping of the sheath from the core. This paper introduces a method to test the anti - stripping property of the core yarn. With a modified Universal Testing Machine, the stripping resistance of friction spun core yarn can be continuously measured. Some factors Influencing the measurements are discussed in detail. The testing results are compared with those from a Y731 Yarn Abrasion Tester and fur - ther confirmed by weaving practice.

酵母菌/锌发酵产物在头皮护理中的功效研究

从人体安全性、控油去屑功效、毛囊养护等方面初步认识酵母菌/锌发酵产物在头皮护理方面的功效。通过人体斑贴试验测试酵母菌/锌发酵产物的人体安全性;使用体外SZ95细胞模型研究酵母菌/锌发酵产物的控油功效,并使用qRT-PCR法验证油脂分泌相关基因SREBP-1c,PPARG和PI3K的表达;采用分光光度法测试酵母菌/锌发酵产物对5α-还原酶的抑制率;通过人体功效测试和离体毛囊模型分别研究酵母菌/锌发酵产物对头皮油脂的抑制效果和对毛囊的养护作用。结果表明:5%含量的酵母菌/锌发酵产物对人体安全隐患较小;0.8%~1.5%含量的酵母菌/锌发酵产物均可显著抑制5α-还原酶的活性;0.5%~2.0%含量的酵母菌/锌发酵产物均可显著抑制体外SZ95细胞油脂的分泌,并显著下调与油脂分泌相关基因SREBP-1c和PPARG的表达,1.0%、2.0%含量的酵母菌/锌发酵产物可显著抑制PI3K的表达;5%含量的酵母菌/锌发酵可显著降低头皮油脂分泌量,并具有改善毛囊周期和促进毛干增长的作用。因此,酵母菌/锌发酵产物具有一定头皮护理功效,在控油、改善毛囊健康、促进毛干生长等方面具有积极作用。

化妆品中去屑剂的研究进展

病理性头皮屑是一种慢性、易复发的头皮疾病,已经成为现代社会困扰人们的常见问题。阐述了微生态失衡、头皮脂质异常分泌和头皮屏障功能受损的三种头皮屑产生机制,并分析了三者之间可能的相互作用关系。同时根据活性成分,对去屑剂进行了分类,并简述了各自的去屑原理。此外,对比分析了各国及地区关于去屑成分的管理要求。最后,介绍了常用去屑剂的功效和安全性,并展望了未来去屑剂的发展趋势,以期为更加科学有效的去屑剂研发提供思路。

生姜6-姜辣素的提取及姜汁去屑洗发水的研制

优选生姜6-姜辣素的提取方法,研究生姜提取液在去屑洗发水中的应用。采用HPLC法,以6-姜辣素为考察指标,分别对榨汁法、加热回流提取法、微波提取法、超声提取法和超声-微波协同萃取法的生姜提取液进行含量测定;通过抑菌实验,考察不同添加量的生姜提取液对洗发香波性能影响,确定生姜提取液的最佳添加量,最小抑菌浓度。结果显示,榨汁法为生姜的最佳提取方法,姜汁的最佳添加量是15%,最小抑菌浓度是:糠秕马拉色菌MIC为18.75 mg/mL,金黄色葡萄球菌、大肠埃希菌和白色念珠菌MIC均为9.375 mg/mL。

2018-2019年国产去屑洗发类化妆品中去屑剂监测结果分析

为了解国产化妆品市场中去屑剂的使用情况,依据《化妆品安全技术规范》(2015版)对2018—2019年生产及流通环节监督抽检的国产去屑洗发类化妆品中水杨酸等五种去屑剂含量进行监测分析。结果发现,在89批次产品中水杨酸、吡硫鎓锌、酮康唑、氯咪巴唑和吡罗克酮乙醇胺盐的检出率分别为12.4%、58.4%、0.0%、11.2%和25.8%,检出质量分数范围分别为0.18%~0.78%、0.066%~1.2%、0%、0.02%~0.4%和0.012%~0.53%。18批产品存在标签标识成分吡硫鎓锌、氯咪巴唑、吡罗克酮乙醇胺盐与检出结果不一致情况。可见,国产去屑洗发类化妆品中五种去屑剂的含量符合国家卫生标准限值要求,标签标识与检出成分不一致情况成为高风险点,应持续加强监督管理。为了提高去屑洗发类化妆品的出口质量,应参考各国化妆品监管标准,适时更新国内化妆品相关规范性技术文件。

HPLC法测定姜汁去屑洗发水中6-姜辣素的含量

建立了高效液相法测定姜汁去屑洗发水中6-姜辣素含量的方法。结果显示,6-姜辣素在0.26滋g～1.58滋g范围内与峰面积积分值呈良好线性关系r(=0.999 9),平均加样回收率为99.15%,相对标准偏差(RSD)为1.50%。

复合喷剂敷料治疗头皮脂溢性皮炎的有效性及安全性研究

目的研究以抗菌肽为主成分的复合喷剂敷料域发去屑洗剂治疗头皮脂溢性皮炎的效果和安全性。方法入组30例轻度至中度脂溢性皮炎患者,入组后第1~7天,每天使用试验产品治疗1次;在第8~28天,每2天使用试验产品治疗1次,分别在治疗的第0天(接受治疗前)、第14天、第28天随访,拍摄临床及皮肤镜照片,通过黏着性头屑10级评分(ASFS)、脂溢性皮炎头皮皮损临床量表、瘙痒评分量表分别评估患者头屑、红斑、丘疹及瘙痒的严重程度,同时要求患者完成头皮自觉症状评分,并填写皮肤病生活质量指数评分(DLQI)问卷。询问并记录研究过程中患者所有不良反应。结果受试者在使用域发去屑洗剂后,头屑明显减少,平均ASFS评分由(11.00±8.094)分降至(2.57±2.22)分(P<0.001),有效率达90%;红斑、丘疹缓解,皮损量表评分由(1.67±0.61)分降至(0.57±0.68)分(P<0.001),有效率达76.7%;瘙痒症状缓解,量表评分由(1.83±0.46)分降至(0.73±0.69)分(P<0.001),有效率达76.7%;患者头皮刺痛、烧灼、油腻的自觉症状亦有不同程度改善,且DLQI显著改善,由(10.47±4.87)分降至(3.53±3.50)分(P<0.05);仅3例患者入眼有轻微刺痛感,可自行好转。结论域发去屑洗剂可有效改善脂溢性皮炎患者头屑、红斑、丘疹及瘙痒症状,安全性良好。

<i>In Vivo</i>and <i>in Vitro</i>Evaluation of Anti Diabetic and Insulin Secretagogue Activities of <i>Capparis zeylanica</i>

Since ancient times, traditional medicines have been in the usage for the treatment of Diabetes mellitus. An edible fruit from traditional medicinal plant Capparis zeylanica (CZ) was studied for its anti diabetic, insulin secretagogue activities and mechanisms involved in it. In Streptozotocin induced diabetes rats, oral administration of Capparis zeylanica methanolic extract (CZME) (200 mg/kg body weight) for 28 days showed a significant reduction in blood glucose levels by 35.53% and enhanced circulating insulin levels by 81.82% than the diabetic control rats. The insulin secretagogue activity mechanisms of the extract were evaluated by using mouse insulinoma beta cell line (MIN6-β). The extract stimulated insulin release in dependent manner of glucose concentration (3 - 16.7 mM) and extract dose (5 - 500 μg/mL). The insulin releasing effect of the extract was significantly enhanced by 3-isobutyl-1-methyl xanthine, glibenclamide, elevated extracellular calcium and K+ depolarized media. This insulin release was significantly reduced in calcium blocking conditions (by nifedipine and EGTA), in the presence of potassium channel opener (diazoxide). Hence, anti diabetic activity of CZME might be a result of its stimulatory effect on insulin release from pancreatic beta cells via KATP channel dependent and independent ways. These results indicate that CZ fruits have the potential to use in diabetes therapy.

Exact Traveling Wave Solutions of Nano-Ionic Solitons and Nano-Ionic Current of MTs Using the exp(-φ (ξ ))-Expansion Method

In this work, the exp(-φ (ξ )) -expansion method is used for the first time to investigate the exact traveling wave solutions involving parameters of nonlinear evolution equations. When these parameters are taken to be special values, the solitary wave solutions are derived from the exact traveling wave solutions. The validity and reliability of the method are tested by its applications to Nano-ionic solitons wave’s propagation along microtubules in living cells and Nano-ionic currents of MTs which play an important role in biology.

黏附性聚多巴胺@己脒定二羟乙基磺酸盐微胶囊的制备及其性能研究

为提高去屑剂己脒定二羟乙基磺酸盐(HD)在湿润环境下的黏附性能,以W_(1)/O/W_(2)双重乳液为模板,合成具有黏附性的聚多巴胺@己脒定二羟乙基磺酸盐微胶囊(PDA@HD微胶囊),并对其形貌结构、载药性能、缓释性能、黏附性能及斑马鱼胚胎急性毒性进行研究。结果表明:PDA@HD微胶囊外观呈球状,有明显核壳结构,平均粒径为353 nm;通过提高双重乳液W_(1)相中HD的质量浓度,可以显著提升PDA@HD微胶囊中HD的搭载率,当HD质量浓度为18 mg/mL,其搭载率为17.1%;HD在PDA@HD微胶囊中的释放表现出明显的pH依赖性,偏酸性环境更有利于HD的释放;PDA@HD微胶囊的黏附力提高明显,其平均黏附力(8.42 nN)约为HD(2.89 nN)的3倍;PDA@HD微胶囊的斑马鱼胚胎急性毒性有所降低,当PDA@HD微胶囊内的HD质量浓度为0.5 mg/mL时,斑马鱼胚胎发育至96 hpf仍无死亡。

抗菌肽在化妆品中的应用研究

从抗菌机理出发,阐述抗菌肽在化妆品中的应用,尤其在祛痘、去屑和口腔产品中的应用。围绕抗菌肽的抗菌效果,从安全性和配伍性等方面进行了系统的阐述。

去屑洗发露中羟吡啶酮的高效液相色谱法检测及质谱确证

该文建立了去屑洗发露中羟吡啶酮的高效液相色谱法(HPLC)测定及其质谱确证方法。样品采用乙腈涡旋超声提取,以950 mL乙腈+50 mL 400 mmol/L草酸溶液作为流动相,经Waters Atlantis Hilic(4.6 mm×250 mm×5μm)色谱柱分离,用二极管阵列检测器(DAD)进行检测。采用硫酸二甲酯对羟吡啶酮衍生化,以正离子多反应监测模式(MRM)进行质谱确证。结果表明,羟吡啶酮在1.0~100.0 mg/L范围内线性关系良好,相关系数(r)为0.999,方法检出限和定量下限分别为8.0μg/g和20.0μg/g,加标回收率为91.8%~96.7%,相对标准偏差(RSD)为1.3%~3.7%。该方法快速、高效、准确,适用于洗发露及吡硫鎓锌原料中羟吡啶酮的测定。使用液相色谱及傅里叶变换红外光谱对羟吡啶酮及其互变异构体2-羟基吡啶-N-氧化物进行了比较,并阐明了洗发露中禁用原料羟吡啶酮的可能来源。

悬液定量法评价去屑剂对马拉色菌抑菌效果的研究

通过建立悬液定量法,利用体外评价法评估去屑剂吡硫鎓锌(ZPT)、氯咪巴唑(甘宝素)、吡罗克酮乙醇胺盐(OCT)的抑菌效果。实验结果表明,不同去屑剂对马拉色菌的抑制速度及效果有显著差异。通过对马拉色菌的抑制率研究发现,不同去屑剂在不同洗护产品中对马拉色菌的抑制效果有剂量与时间的依赖性,而短时间内均没有使马拉色菌大幅度减少,由此推测去屑剂需要通过沉积在头皮上,或者反复沉积,才能长时间作用起到去屑功效。