基于3,5-二溴水杨醛席夫碱镍配合物-氧化石墨烯电化学免疫传感器检测Anti-IgG含量的研究

应用物理吸附法将羊抗人IgG抗原直接固定于3,5-二溴水杨醛席夫碱镍配合物-氧化石墨烯修饰的金电极表面,制备电化学免疫传感器。采用循环伏安法和交流阻抗法对传感器进行表征,结果表明该传感器适合检测Anti-IgG浓度。同时探讨了缓冲液pH值、扫描速度、免疫反应温度、抗原与抗体配比对循环伏安峰电流的影响,结果表明在5-100mV/s扫速范围内,峰电流与扫速呈线性。孵育最优条件为25℃,h-IgG与Anti-IgG配比为1∶1。循环伏安法研究还表明Anti-IgG浓度在0.01-260μg/L范围内,线性关系良好,相关系数r^2=0.993,检出限（S/N=3）为0.006μg/L,据此建立了检测Anti-IgG浓度的新方法。

信号分子IRAK1/4在antiβ-2GPI/β2GPI复合物诱导THP-1细胞表达TF中的作用探讨

目的:探讨IRAK1和IRAK4在antiβ-2GPI/β2GPI复合物诱导单核细胞株THP-1表达组织因子(TF)中的作用。方法:利用荧光定量PCR(Real-time PCR)、TF活性试剂盒分别检测THP-1细胞表达TF mRNA及TF活性;Western blot检测anti-β2GPI/β2GPI复合物诱导THP-1细胞表达IRAK1、磷酸化-IRAK1(p-IRAK1)、IRAK4情况;观察IRAK1/4抑制物是否干预anti-β2GPI/β2GPI复合物诱导THP-1表达TF。结果:Antiβ-2GPI/β2GPI复合物(100μg/ml)诱导THP-1细胞表达TF显著增加(P<0.05 vs control);Antiβ-2GPI/β2GPI复合物(100μg/ml)刺激THP-1细胞表达IRAK1、p-IRAK1、IRAK4(蛋白)显著升高(P<0.05 vscontrol);IRAK1/4抑制物(50μmol/L)能够阻断antiβ-2GPI/β2GPI复合物(100μg/ml)诱导THP-1表达TF及IRAK1磷酸化的效应。结论:antiβ-2GPI/β2GPI复合物诱导THP-1细胞表达TF过程中,信号分子IRAK1/4被激活进而发挥重要作用。

三维Anti de Sitter空间中Lorentzian曲面的S_t^1×S_s^1-值光锥Gauss映射的奇点分类

利用Arnol'd的Legendrian理论,对三维Anti de Sitter空间中Lorentzian曲面进行了研究.引入光维高度函数概念研究了三维Anti de Sitter空间Lorentzian曲面的S1t×S1s-值、光锥Gauss映射的奇点,进行了奇点分类,揭示了类光Causs-kronecker曲率之间的关系;并研究了Lorentzian曲面的一些基本几何性质.

Reverse Solution and Parametric Design of the Conjugate Cam Weft Insertion Mechanism Based on VB.NET and UG

In order to realize the parametric design of the conjugate cam weft insertion mechanism,according to the necessary parameters of weft insertion process,ideal kinematic curves of the weft insertion mechanism were given,and the mathematical model of reverse solution for this mechanism was established.The parameters of this mechanism were obtained by reverse solution on the basis of the given ideal kinematic curves and kinematic requirements.The parametric design platform which was integrated with the functions of parametric reverse solution,motion simulation,three-dimensional modeling and virtual assembly was developed based on VB.NET and Unigraphics(UG) NX.After entering the technological parameters of weft insertion process and essential structural parameters by users,three-dimensional drawing of the main parts such as the conjugate cam and shaft,can be obtained by this platform,also the processing data of the cam can be calculated.This platform provides a rapid approach for parametric design of weft insertion mechanism.

On the Construction of Deepening Anti - corruption Mechanism

It needs the foundation of system and the guarantee of organizational system for anti-corruption,but it is more necessary to build and form an effective anti-corruption mechanism,so that the anti-corruption can be really put into practice. Anti-corruption mechanism refers to a organic operation system of the interaction,interconnection and constraint between the constituent elements( parts) and elements of national anti-corruption,and as a system,anti-corruption mechanism should have the characteristics of system aticness,comprehensiveness,transparency,legalization,public participation,scientific dynam ic,and internationalism. The construction of deepening anti-corruption mechanism is the need for reconstructing the ruling legitimacy of the party and the governm ent. Adhering to the principle of treating both root causes and symptoms is necessary in the construction of anti-corruption m echanism,com bating and punishing corruption is an important part of anti-corruption,and the prevention and control of corruption is the basic project of anti-corruption. Therefore,the construction of prevention and control mechanism in the anti-corruption mechanism has a more far-reaching significance.

Anti-Bacterial and Flame Retardant for Cotton Fabric by One-Bath Finishing

A research on the process of cotton fabric flame-re-tarding,anti-bacterial finishing and one-bath finish-ing of anti-bacterial and flame-retarding is discussed.The flame retardant agent was phosphorous-contained,and the bacteriostatic finishing agent named SFR-1 wassynthesized.The flame retardancy of the fabric finishedcan meet the DOC FF3-71 Children Sleepwear Stan-dard.Its bacterial inhibiting capacity can meet and ex-ceed the requirements of similar products

Testing of Anti-stripping Property of Friction Spun Core Yarn

Friction spun core yarn has two components: filament core and staple fiber sheath. Under axial rubbing action, the failure mode of the core yarn is the stripping of the sheath from the core. This paper introduces a method to test the anti - stripping property of the core yarn. With a modified Universal Testing Machine, the stripping resistance of friction spun core yarn can be continuously measured. Some factors Influencing the measurements are discussed in detail. The testing results are compared with those from a Y731 Yarn Abrasion Tester and fur - ther confirmed by weaving practice.

抗肿瘤药说明书安全性信息上市后修订的考虑要点

目的介绍抗肿瘤药说明书安全性信息上市后修订的考虑要点,为药品上市许可持有人提供参考。方法基于国家药品监督管理局药品评价中心(简称“评价中心”)近年的修订实例,阐述修订目的、启动原因、决策证据以及修订原则。结果与结论说明书安全性信息修订是一项系统工程。评价中心将持续致力于完善包括抗肿瘤药在内的各种药品说明书安全性信息。药品上市许可持有人应充分发挥主体责任,及时、主动完善说明书,保障人民群众用药安全。

Influence of Thermal Load on Mechanical Property of Cemented Carbide Material and Heavy Cemented Carbide Inserts Life

A large amount of cutting heat is produced during the heavy cutting process,and insert life is restricted by the effect of thermal load.The thermal load experiment of cemented carbide SCS,WF and YT15 is carried out,and the results show that the bending strength and fracture toughness of cemented carbide material decrease obviously under cyclic thermal load,while in the cooling process,the material mechanical property changes worse suddenly.The high-temperature mechanical property of SCS is the most stable,and that of YT15 is the worst.Further,a relation model among cutting temperature,cutting parameters and insert life is established.Finally,the measures to improve heavy cemented carbide inserts life are summarized from the aspects of cutting parameters selection,insert optimization design and TiCN,Al2O3,TiN complex insert coating.The research results are expected to provide support and reference for heavy cutting technology and insert technology.

<i>In Vivo</i>and <i>in Vitro</i>Evaluation of Anti Diabetic and Insulin Secretagogue Activities of <i>Capparis zeylanica</i>

Since ancient times, traditional medicines have been in the usage for the treatment of Diabetes mellitus. An edible fruit from traditional medicinal plant Capparis zeylanica (CZ) was studied for its anti diabetic, insulin secretagogue activities and mechanisms involved in it. In Streptozotocin induced diabetes rats, oral administration of Capparis zeylanica methanolic extract (CZME) (200 mg/kg body weight) for 28 days showed a significant reduction in blood glucose levels by 35.53% and enhanced circulating insulin levels by 81.82% than the diabetic control rats. The insulin secretagogue activity mechanisms of the extract were evaluated by using mouse insulinoma beta cell line (MIN6-β). The extract stimulated insulin release in dependent manner of glucose concentration (3 - 16.7 mM) and extract dose (5 - 500 μg/mL). The insulin releasing effect of the extract was significantly enhanced by 3-isobutyl-1-methyl xanthine, glibenclamide, elevated extracellular calcium and K+ depolarized media. This insulin release was significantly reduced in calcium blocking conditions (by nifedipine and EGTA), in the presence of potassium channel opener (diazoxide). Hence, anti diabetic activity of CZME might be a result of its stimulatory effect on insulin release from pancreatic beta cells via KATP channel dependent and independent ways. These results indicate that CZ fruits have the potential to use in diabetes therapy.

Exact Traveling Wave Solutions of Nano-Ionic Solitons and Nano-Ionic Current of MTs Using the exp(-φ (ξ ))-Expansion Method

In this work, the exp(-φ (ξ )) -expansion method is used for the first time to investigate the exact traveling wave solutions involving parameters of nonlinear evolution equations. When these parameters are taken to be special values, the solitary wave solutions are derived from the exact traveling wave solutions. The validity and reliability of the method are tested by its applications to Nano-ionic solitons wave’s propagation along microtubules in living cells and Nano-ionic currents of MTs which play an important role in biology.

抗干扰跳频滤波器的小型化仿真设计

为了提升装备在复杂电磁环境下的生存能力,解决手持式通信终端在强电磁干扰下通信质量差、体积笨重等问题,该文提出一种抗电磁干扰的跳频滤波器的仿真和设计方案。该模型基于双调谐LC滤波单元和变容二极管工作原理,通过采用等效电路变换和等效电路吸收等手段,简化电路模型和优化电路布板,设计了频率范围在225~525 MHz的微型跳频滤波器,并使用先进设计系统(ADS)进行了仿真验证,在满足插入损耗、频率选择性、带外抑制等关键指标下实现了抗干扰跳频滤波器尺寸的小型化设计。

高线小规格防倒插的方法研究

针对高线小规格硬线钢在斯太尔摩风冷线上倒插问题的产生,导致生产线作业率和成材率下降等问题,通过调整吐丝机设备安装及参数,合理分配散冷辊道参数及设备,确保高线小规格硬线钢在斯太尔摩风冷线上的稳定性,进而提高了成品质量。

考虑安全系数的坑中坑开挖基坑失稳模式分析

坑中坑基坑是工程中较为普遍的一种基坑形式,基坑开挖后再次开挖引起的应力释放,对基坑变形及整体稳定性造成不同影响。文章以南京某快速路坑中坑基坑为工程背景,结合Plaxis有限元计算,采用Hardening-Soil土体本构模型,考虑基坑抗隆起安全系数,分析外墙插入比λ与坑趾系数α对基坑稳定系数的影响,揭示坑中坑基坑两种失稳破坏模式,结果表明,当λ>0.25或α<1.5时,内外基坑失稳时形成贯通的圆弧滑动面,且安全稳定系数随λ增大和α的减小而增大,但当α<1.0时,外坑台宽的减小会使滑动圆弧面缩小,承受抗滑土体的被动土范围减少,安全系数减小;λ<0.25或α>1.5时,基坑隆起破坏点出现在内外坑不同位置,内外基坑可视为两个独立基坑进行稳定验算。研究成果可为坑中坑基坑稳定性验算与支护提供参考。

换热器防垢除垢实验研究

考察了二甲基甲酰胺中的盐在换热器换热冷却过程中的结垢行为,分别采用内置弹簧和液固流态化以及两者结合的方法对换热器在线防垢除垢进行研究。采用测定总传热系数法研究污垢热阻随时间的变化规律,并用污垢称重法测定不同条件的总污垢量。研究了流体流速、传热温差、弹簧结构、惰性固体颗粒数量和大小等诸因素对结垢的影响,结果表明:直径与管内径比为0.8,螺距和管内径比为1.5的弹簧在0.6～1.0m·s-1的流速范围内防垢除垢效果最佳,随着颗粒数量的增加和粒径的增大,污垢生成速率降低,结垢量减少,相比同条件下单纯颗粒流态化除垢,内置弹簧和颗粒流态化结合法可以使污垢量减少20%～50%。实验研究结果对实际的工业过程具有一定的参考意义和指导价值。

换热器内置弹簧脉冲流动防垢除垢实验研究

以二甲基甲酰胺生产中易于结垢的换热器冷却过程为研究对象,采用换热管内置弹簧并利用泵的开、停措施使流体产生脉冲流动的方法对换热器在线防垢除垢进行研究。采用测定换热管外冷却水的吸热量随时间的变化关系以及试验结束后管内污垢称重法研究内置弹簧脉冲流动法对换热器在线防垢除垢效果。研究了泵的开、停时间,内置弹簧长度等因素对防垢除垢的影响。结果表明:在试验装置上,内置弹簧长度175～198cm、泵运行时间5～10s、泵停止时间5s时,具有显著的防垢除垢效果,在试验装置运行4h除垢率高达92.9%。同时在此条件下装置运行4～12h内,污垢增长量较少,说明此法除垢效果稳定。实验研究结果对实际工业换热器的改造具有一定的参考意义和指导价值。

抗冲击金刚石圆锯片结构的有限元分析

采用ANSYS有限元分析软件 ,对节块前方加焊不同负倾角护齿插件的金刚石圆锯片进行变形和应力计算 ,得出最佳的护齿插件锯片结构 ,并与普通圆锯片比较 ,可知在相同载荷作用下 ,合理设置插件倾角 ,可使最大变形、应力值大大减小 ,应力均衡 ,应力集中区明显改善 ,表明护齿插件提高了锯片基体的刚度和节块的抗冲击能力 。

插层改性累托石对水性环氧涂料性能的影响

根据累托石可进行阳离子交换的矿物学特征,先将天然累托石钠基化,再用氟碳表面活性剂和复合季铵盐对其进行插层改性,使有机铵阳离子进入累托石层间,并使层间距由2.41nm撑开至4.75nm,将插层改性后的累托石用作水性环氧涂料的防沉剂和流变助剂。结果表明:当插层改性累托石用量为颜填料质量的2%时,可以显著改善涂料的流平性、抗流挂性和颜填料的分散性。

移动插入式灌水器的结构研究与抗堵塞试验分析

通过对现有滴头、滴箭等产品结构的研究、改进与优化,设计开发出一种抗堵塞功能较强的插入式地下灌水器产品,整体上具有快速装拆、移动灵活、滴流量偏差小、水力性能稳定等特点。为检验其抗堵塞功能的优劣,分别进行了自由出流和插地出流条件的滴水试验。结果表明:移动插入式灌水器出流均匀,具有较强的抗堵塞性能,自由出流时的平均滴水量与多次间歇式插地出流时的平均滴水量相当,其波动范围在5%以内,较适合于在移动式地下滴灌系统中应用。

Boost电感反相绕组法的共模噪声抑制特性分析与设计

反相绕组法是抑制Boost变换器传导共模噪声的有效方法,但影响反相绕组法噪声抑制能力的因素很多,机理复杂,目前尚缺乏明确的反相绕组法优化设计的方法。针对大匝比反相绕组法,通过对共模噪声抑制机理的深入分析,建立大匝比反相绕组法的插入损耗数学模型。基于插入损耗数学模型,明确补偿电容、漏感、反相绕组匝数是影响大匝比反相绕组法噪声抑制能力的关键因素。在此基础上,提出基于低频段共模噪声抑制效果最优原则的大匝比反相绕组法的设计方法。通过优选补偿电容、减小漏感、增大反相绕组等效匝比与漏感比值的方法,可扩大有效频段带宽,极大改善大匝比反相绕组法共模噪声抑制能力。实验结果表明,提出的大匝比反相绕组法可有效抑制Boost电路传导共模噪声。