Blood glucose changes surrounding initiation of tumor-necrosis factor inhibitors and conventional disease-modifying anti-rheumatic drugs in veterans with rheumatoid arthritis

AIM To determine the scope of acute hypoglycemic effects for certain anti-rheumatic medications in a large retrospective observational study. METHODS Patients enrolled in the Veterans Affairs Rheumatoid Arthritis (VARA) registry were selected who, during follow-up, initiated treatment with tumor necrosis factor inhibitors (TNFi's, including etanercept, adalimumab, infliximab, golimumab, or certolizumab), prednisone, or conventional disease-modifying anti-rheumatic drugs(DMARDs), and for whom proximate random blood glucose (RBG) measurements were available within a window 2-wk prior to, and 6 mo following, medication initiation. Similar data were obtained for patients with proximate values available for glycosylated hemoglobin A1C values within a window 2 mo preceding, and 12 mo following, medication initiation. RBG and A1C measurements were compared before and after initiation events using paired t-tests, and multivariate regression analysis was performed including established comorbidities and demographics.RESULTS Two thousands one hundred and eleven patients contributed at least one proximate measurement surrounding the initiation of any examined medication. A significant decrease in RBG was noted surrounding 653 individual hydroxychloroquine-initiation events(-3.68 mg/dL, P = 0.04), while an increase was noted for RBG surrounding 665 prednisone-initiation events(+5.85 mg/d L, P < 0.01). A statistically significant decrease in A1C was noted for sulfasalazine initiation, as measured by 49 individual initiation events(-0.70%, P < 0.01). Multivariate regression analyses, using methotrexate as the referent, suggest sulfasalazine (β =-0.58, P = 0.01) and hydroxychloroquine(β =-5.78, P = 0.01) use as predictors of lower post-medicationinitiation RBG and A1C values, respectively. Analysis by drug class suggested prednisone (or glucocorticoids) as predictive of higher medication-initiation event RBG among all start events as compared to DMARDs, while this analysis did not show any drug class-level effect for TNFi. A diagnosis of congestive heart failure(β = 4.69, P = 0.03) was predictive for higher post-initiation RBG values among all medication-initiation events.CONCLUSION No statistically significant hypoglycemic effects surrounding TNFi initiation were observed in this large cohort. Sulfasalazine and hydroxychloroquine may have epidemiologically significant acute hypoglycemic effects.

基于3,5-二溴水杨醛席夫碱镍配合物-氧化石墨烯电化学免疫传感器检测Anti-IgG含量的研究

应用物理吸附法将羊抗人IgG抗原直接固定于3,5-二溴水杨醛席夫碱镍配合物-氧化石墨烯修饰的金电极表面,制备电化学免疫传感器。采用循环伏安法和交流阻抗法对传感器进行表征,结果表明该传感器适合检测Anti-IgG浓度。同时探讨了缓冲液pH值、扫描速度、免疫反应温度、抗原与抗体配比对循环伏安峰电流的影响,结果表明在5-100mV/s扫速范围内,峰电流与扫速呈线性。孵育最优条件为25℃,h-IgG与Anti-IgG配比为1∶1。循环伏安法研究还表明Anti-IgG浓度在0.01-260μg/L范围内,线性关系良好,相关系数r^2=0.993,检出限（S/N=3）为0.006μg/L,据此建立了检测Anti-IgG浓度的新方法。

血清CRP、Anti-CCP及RF检测在类风湿关节炎诊断中的应用

目的：探讨C反应蛋白、抗环瓜氨酸肽抗体及类风湿因子在类风湿关节炎诊断中的应用。方法：选择2011-09～2013-09来我院就诊的类风湿关节炎患者100例,关节疼痛的非类风湿关节炎患者100例,分别用乳胶增强免疫比浊法测定C反应蛋白和类风湿因子,用酶联免疫吸附试验测定抗环瓜氨酸肽抗体,分析两组患者中C反应蛋白、抗环瓜氨酸肽抗体及类风湿因子的相关性。结果：C反应蛋白对类风湿关节炎的敏感性为67%,特异性为83%,抗环瓜氨酸肽抗体对类风湿关节炎的敏感性为63%,特异性为97%,类风湿因子对类风湿关节炎的敏感性为86%,特异性为75%,C反应蛋白、抗环瓜氨酸肽抗体及类风湿因子三者联合检测的敏感性为63%,特异性为100%。结论：在类风湿关节炎的诊断中,C反应蛋白的敏感性高于类风湿因子和抗环瓜氨酸肽抗体,但特异性低于类风湿因子和抗环瓜氨酸肽抗体,三者联合检测有助于类风湿关节炎的早期诊断和病情控制。

信号分子IRAK1/4在antiβ-2GPI/β2GPI复合物诱导THP-1细胞表达TF中的作用探讨

目的:探讨IRAK1和IRAK4在antiβ-2GPI/β2GPI复合物诱导单核细胞株THP-1表达组织因子(TF)中的作用。方法:利用荧光定量PCR(Real-time PCR)、TF活性试剂盒分别检测THP-1细胞表达TF mRNA及TF活性;Western blot检测anti-β2GPI/β2GPI复合物诱导THP-1细胞表达IRAK1、磷酸化-IRAK1(p-IRAK1)、IRAK4情况;观察IRAK1/4抑制物是否干预anti-β2GPI/β2GPI复合物诱导THP-1表达TF。结果:Antiβ-2GPI/β2GPI复合物(100μg/ml)诱导THP-1细胞表达TF显著增加(P<0.05 vs control);Antiβ-2GPI/β2GPI复合物(100μg/ml)刺激THP-1细胞表达IRAK1、p-IRAK1、IRAK4(蛋白)显著升高(P<0.05 vscontrol);IRAK1/4抑制物(50μmol/L)能够阻断antiβ-2GPI/β2GPI复合物(100μg/ml)诱导THP-1表达TF及IRAK1磷酸化的效应。结论:antiβ-2GPI/β2GPI复合物诱导THP-1细胞表达TF过程中,信号分子IRAK1/4被激活进而发挥重要作用。

三维Anti de Sitter空间中Lorentzian曲面的S_t^1×S_s^1-值光锥Gauss映射的奇点分类

利用Arnol'd的Legendrian理论,对三维Anti de Sitter空间中Lorentzian曲面进行了研究.引入光维高度函数概念研究了三维Anti de Sitter空间Lorentzian曲面的S1t×S1s-值、光锥Gauss映射的奇点,进行了奇点分类,揭示了类光Causs-kronecker曲率之间的关系;并研究了Lorentzian曲面的一些基本几何性质.

On the Construction of Deepening Anti - corruption Mechanism

It needs the foundation of system and the guarantee of organizational system for anti-corruption,but it is more necessary to build and form an effective anti-corruption mechanism,so that the anti-corruption can be really put into practice. Anti-corruption mechanism refers to a organic operation system of the interaction,interconnection and constraint between the constituent elements( parts) and elements of national anti-corruption,and as a system,anti-corruption mechanism should have the characteristics of system aticness,comprehensiveness,transparency,legalization,public participation,scientific dynam ic,and internationalism. The construction of deepening anti-corruption mechanism is the need for reconstructing the ruling legitimacy of the party and the governm ent. Adhering to the principle of treating both root causes and symptoms is necessary in the construction of anti-corruption m echanism,com bating and punishing corruption is an important part of anti-corruption,and the prevention and control of corruption is the basic project of anti-corruption. Therefore,the construction of prevention and control mechanism in the anti-corruption mechanism has a more far-reaching significance.

Contribution to the Valorization of Plants Used in the Management of Rheumatic Diseases in Burkina Faso

Joint pain is a real public health problem. According to recent studies, it is increasingly common in sub-Saharan Africa and represents a cause of greater loss of working days than accidents, heart disease and infections. The aim of this study was to study the pharmacochemical and therapeutic evidence of ten (10) plants used in the management of joint pain by traditional health practitioners in Ouagadougou. We conducted a meta-analysis of the data from December 2018 to September 2019. The anti-inflammatory activity of the ten (10) plants retained the maximum consensus internationally. Trichilia emetica Vahl (35.25%), Guiera senegalensis J.F.G.mel. (31%), Calotropis procera (Ait) Ait.F. (28.29%) and Crescientia cujete L. (25.12%) had more than 25% internationally reliability for their effects on joint pain. The structural analogy between conventional drugs and the molecules found in these plants has been confirmed. Additional studies should be carried out in order to improve and secure the use of these plants, which represent a prominent option in the therapeutic offer.

Anti-Bacterial and Flame Retardant for Cotton Fabric by One-Bath Finishing

A research on the process of cotton fabric flame-re-tarding,anti-bacterial finishing and one-bath finish-ing of anti-bacterial and flame-retarding is discussed.The flame retardant agent was phosphorous-contained,and the bacteriostatic finishing agent named SFR-1 wassynthesized.The flame retardancy of the fabric finishedcan meet the DOC FF3-71 Children Sleepwear Stan-dard.Its bacterial inhibiting capacity can meet and ex-ceed the requirements of similar products

Testing of Anti-stripping Property of Friction Spun Core Yarn

Friction spun core yarn has two components: filament core and staple fiber sheath. Under axial rubbing action, the failure mode of the core yarn is the stripping of the sheath from the core. This paper introduces a method to test the anti - stripping property of the core yarn. With a modified Universal Testing Machine, the stripping resistance of friction spun core yarn can be continuously measured. Some factors Influencing the measurements are discussed in detail. The testing results are compared with those from a Y731 Yarn Abrasion Tester and fur - ther confirmed by weaving practice.

抗风湿类中成药和保健食品中非法添加化学成分的高液相色谱-质谱/质谱法检测

目的建立一种快速检测抗风湿类中成药和保健食品中30种非法添加化学成分的高液相色谱-质谱/质谱法。方法色谱条件：C18（4.6 mm×250 mm,5μm）色谱柱,以甲醇-0.1%乙酸溶液为流动相,梯度洗脱,质谱条件：电喷雾离子化正离子检测（ESI＋）,全扫描一级质谱、选择离子二级扫描质谱检测方式,扫描范围50-800 aum,对抗风湿类中成药和保健食品中非法添加的化学成分进行快速定性分析。结果根据检测到的化合物的色谱保留时间及一级、二级质谱信息,并与对照品比较,可作为分析抗风湿类中成药和保健食品中非法添加吗啡、甲氧苄啶、对乙酰氨基酚、罗通定、氨基比林、龙胆苦苷、磺胺甲恶唑、安替比林、阿司匹林、非那西丁、盐酸美沙酮、泼尼松、可的松、异丙安替比林、吡罗昔康、氢化可的松、金诺芬、地塞米松、甲基泼尼松龙、醋酸曲安奈德、酮洛芬、舒林酸、萘普生、地西泮、醋酸地塞米松、保泰松、奥沙普秦、双氯芬酸钠、吲哚美辛和丙酸倍氯米松等30种化学成分的有效手段。在50批供试品中,检测到有10种供试品中含有非法添加的化学成分。结论本方法专属性强、灵敏度高,可作为抗风湿类中成药和保健食品中非法添加化学成分的快速检测方法。

超高效液相色谱-四极杆飞行时间质谱测定抗风湿类中药非法添加的化学药物成分

目的:建立快速、准确、高灵敏度的测定抗风湿类中药制剂中非法添加醋酸泼尼松、萘普生、醋酸地塞米松、双氯芬酸钠、吲哚美辛和布洛芬分析方法。方法:采用Waters ACQUITY BEH C18色谱柱(2.1 mm×50mm,1.7μm),以甲醇溶液为流动相A,0.1%醋酸的水溶液为流动相B,梯度洗脱。流速0.4 mL(min-1,Waters液相色谱-四级杆/飞行时间质谱,电喷雾离子源(ESI),毛细管电压为-2500 V,检测方式为负离子模式,一级、二级同时扫描。通过比较样品峰与对照品峰的保留时间、一级质谱和二级质谱碎片离子,确定样品中是否掺杂化学抗风湿药物。结果:建立的超高效液相色谱-串联质谱方法检测上述化学药物成分不受药物辅料干扰,各色谱峰分离良好,17批受试制剂中未检测到掺有上述药物。结论:此方法灵敏准确,可作为抗风湿类中药制剂中非法添加醋酸泼尼松、萘普生、醋酸地塞米松、双氯芬酸钠、吲哚美辛和布洛芬的有效检测方法。

238例血清抗Ro-52抗体阳性分析

目的探讨抗Ro-52抗体在不同疾病中的临床应用价值及意义。方法采用欧蒙免疫印迹法检测患者血清抗Ro-52抗体,回顾性分析238例阳性结果构成比及与其他自身抗体的关系。结果 238例阳性结果中,构成比较高分别为系统性红斑狼疮23.5%,类风湿性关节炎16.8%和干燥综合征16.0%。抗Ro-52常伴随抗SSA、抗SSB及抗RNP出现,主要见于SLE、SS及RA。结论自身免疫性疾病的起始和发病机制尚不清楚,其诊断和鉴别诊断也较为复杂。抗Ro-52抗体可存在多种疾病,仅能作为患者存在自身免疫疾病可能的提示,并不具备高特异性。但作为高阳性率自身抗体,在联合检测中具有一定的辅助诊断及鉴别诊断价值。

斑点印迹法检测抗-Jo-1抗体的临床研究

目的建立简便可靠的检测抗-Jo-1抗体的方法。方法用丙酮沉淀兔胸腺中的总蛋白制成兔胸腺丙酮粉,从兔胸腺丙酮粉中提取可提取性核抗原(ENA),ENA经亲和色谱纯化出Jo-1抗原,将纯化的Jo-1抗原制备成斑点印迹(DB)试剂盒,用该试剂盒检测多种风湿免疫性疾病和正常血清中的抗-Jo-1抗体,探讨各种血清中抗-Jo-1抗体的阳性率。结果 DB试剂盒共检测92份血清中的抗-Jo-1抗体。各种风湿性疾病血清69份,其中多发性肌炎(DM)20份,阳性6份(30%);其他风湿性疾病49份,无阳性。健康对照23份,无阳性。该试剂盒对诊断DM的敏感度为30%,特异度为100%。结论 DB试剂盒检测抗-Jo-1抗体方法简便,结果可靠,具有较大的临床开发应用价值。

过氧化物酶-抗过氧化物酶复合物(PAP)检测血清抗核抗体

目的 探讨过氧化物酶 -抗过氧化物酶复合物 (PAP)检测血清抗核抗体 (ANA)的实用性。方法 以小鼠肝细胞为基质 ,对 PAP方法测定血清 ANA的滴度、核型进行了方法学检测 ,并同时与间接免疫荧光法相比较 ,测定了 2 0 3例正常人及 46例风湿病患者的血清 ANA。结果 PAP法敏感性高于 IIF法 ,滴度是 IIF法的 1 0～ 6 4 0倍。PAP可提高血清 ANA的阳性检出率 ,还可提高核型的清晰度。结论 PAP法适用于缺乏荧光显微镜的基层医院开展 ANA的检测。

液相色谱-离子阱质谱联用法检测抗风湿类中药制剂中非法掺入的萘普生、吲哚美辛

目的:建市检测抗风湿类中成药中非法掺入萘普生、吲哚美辛的专属性方法。方法:采用液相色谱-电喷雾质谱联用法。液相色谱:色谱柱为 Agilent C_(18)柱(150 mm×4.6 mm,5 μm),流动相为0.02 moL·L^(-1)醋酸铵-1%醋酸溶液-甲醇(32:3:65),流速0.4 mL·min^(-1);柱温30℃。质谱:电喷雾电离源(ESI)负离子检测;质荷比范围 m/z 100～400,干燥气温度350℃;干燥气流速10 L·min^(-1);干燥气压力276 kPa;源电压为5 kV。通过相对分子质量、一级及二级质谱碎片信息、液相色谱保留时间和紫外光潜四方面的信息,对抗风湿类中成药的提取液中非法掺入的萘普生、吲哚美辛进行定性鉴别。结果:6批抗风湿类中药,3批检出掺有萘普牛,其中2批检出同时掺有吲哚美辛。结论:该方法选择性强,灵敏度高,可作为分析检测抗风湿类中成药中萘普生、吲哚美辛的有效方法,可用于分析鉴定中成药非法添加西药成分的打假工作。

UPLC-MS/MS法快速筛查抗风湿类中药贴剂中非法添加的17种化学药物成分

目的:建立快速筛查抗风湿类中药贴剂中非法添加的9种糖皮质激素和8种非甾体抗炎药物成分的方法。方法:采用超高效液相色谱-串联质谱法(UPLC-MS/MS)。色谱条件:色谱柱Acquity UPLC~?BEN C_(18),流动相为甲醇-水(90∶10,V/V),流速为0.4 m L/min,柱温为30℃,进样量为1μL;质谱条件:离子源为电喷雾离子源,喷雾针电压为5 500 V,离子源喷雾气GS1、GS2分别为50、50 psi,离子源温度为500℃,扫描范围为m/z 50~550,检测方式为正负离子模式,多反应监测(MRM)联合相关信息扫描(IDA)和增强离子扫描(EPI)模式。结果:17种化学药物成分检测限均≤0.400μg/m L。精密度、重复性、稳定性试验的RSD分别≤4.93%、7.69%、9.57%。实测样品中可检出双氯芬酸钠。结论:该方法专属性强、灵敏度高,适用于抗风湿类中药贴剂中非法添加化学药物成分的定性分析。

蒙药复方森登-4研究概述

蒙药森登-4是蒙医临床上常用的抗风湿药,用于治疗关节炎、水肿等病证。从化学成分、质量控制、工艺研究、药理作用、安全性、适应证候等方面归纳总结森登-4味汤的研究现状,为进一步研究蒙药森登-4味汤提供参考依据。

UPLC-MS/MS法检测抗风湿类中药制剂中非法添加的21种糖皮质激素类化学药物

目的建立快速、准确、灵敏检测抗风湿类中药制剂中非法添加的21种糖皮质激素类化学药物的分析方法。方法采用Eclipse Plus-C_(18)(2.1mm×100mm,1.8μm)色谱柱,流动相为0.1%乙酸溶液-乙腈,线性梯度洗脱,流速0.3mL·min^(-1),柱温20℃,进样量5μL,离子源为ESI源,正离子扫描模式,多反应监测测定。结果各化合物在10~500ng·mL^(-1)与峰面积线性关系良好(r>0.999),其平均回收率为93.0%~99.6%,RSD为1.2%~2.9%,检出限为0.08~0.50ng·mL^(-1)。结论该方法能满足检测抗风湿类中药制剂中21种糖皮质激素类化学药物的需要。

超高效液相色谱-串联质谱法检测茶制品中非法添加13种抗风湿类药物

目的建立超高效液相色谱-串联质谱法检测茶制品中非法添加13种抗风湿类药物(氨基比林、对乙酰氨基酚、甲氧苄啶、氢化可的松、吡罗昔康、醋酸泼尼松、地塞米松、双氯芬酸钠、吲哚美辛、保泰松、罗通定、安替比林、非那西丁)的分析方法。方法样品甲醇超声提取后,以0.1%的甲酸溶液-乙腈为流动相梯度洗脱,经InertsilODS-3(2.1mm×75mm,2μm)色谱柱分离,目标化合物在ESI源正离子模式下电离,采用多反应监测模式进行定性、定量分析。结果13种抗风湿的方法检出限(S/N=3)为0.3~1.6μg/kg,定量限(S/N=10)为1.4~6.5μg/kg。在低、中、高3个加标水平下的平均回收率为80.20%~110.31%。对25批次市售抗风湿类茶制品进行检测,均未发现上述13种抗风湿类药物。结论该方法灵敏高、专属性强,适用于茶制品中抗风湿类非法添加的定量及确证分析。

<i>In Vivo</i>and <i>in Vitro</i>Evaluation of Anti Diabetic and Insulin Secretagogue Activities of <i>Capparis zeylanica</i>

Since ancient times, traditional medicines have been in the usage for the treatment of Diabetes mellitus. An edible fruit from traditional medicinal plant Capparis zeylanica (CZ) was studied for its anti diabetic, insulin secretagogue activities and mechanisms involved in it. In Streptozotocin induced diabetes rats, oral administration of Capparis zeylanica methanolic extract (CZME) (200 mg/kg body weight) for 28 days showed a significant reduction in blood glucose levels by 35.53% and enhanced circulating insulin levels by 81.82% than the diabetic control rats. The insulin secretagogue activity mechanisms of the extract were evaluated by using mouse insulinoma beta cell line (MIN6-β). The extract stimulated insulin release in dependent manner of glucose concentration (3 - 16.7 mM) and extract dose (5 - 500 μg/mL). The insulin releasing effect of the extract was significantly enhanced by 3-isobutyl-1-methyl xanthine, glibenclamide, elevated extracellular calcium and K+ depolarized media. This insulin release was significantly reduced in calcium blocking conditions (by nifedipine and EGTA), in the presence of potassium channel opener (diazoxide). Hence, anti diabetic activity of CZME might be a result of its stimulatory effect on insulin release from pancreatic beta cells via KATP channel dependent and independent ways. These results indicate that CZ fruits have the potential to use in diabetes therapy.