Chromatographic finger print analysis of anti-inflammatory active extract fractions of aerial parts of Tribulus terrestris by HPTLC technique

Objective:To develop HPTLC fingerprint profile of anti-inflammatory active extract fractions of Tribulus terrestris(family Zygophyllaceae).Methods:The anti-inflammatory activity was tested for the methanol and its fractions(chloroform,ethyl acetate,n-butanol and aqueous)and chloroform extract of Tribulus terrestris(aerial parts)by injecting different groups of rats(6 each)with carrageenan in hind paw and measuring the edema volume before and 1,2 and 3 h after carrageenan injection.Control group received saline i.p.The extracts treatment was injected i.p.in doses of 200 mg/kg 1 h before carrageenan administration.Indomethacin(30 mg/kg)was used as standard.HPTLC studies were carried out using CAMAG HPTLC system equipped with Linomat IV applicator.TLC scanner 3.Reprostar 3,CAMAG ADC 2 and WIN CATS-4 software for the active fractions of chloroform fraction of methanol extract.Results:The methanol extract showed good antiedematous effect with percentage of inhibition more than 72%,indicating its ability to inhibit the inflammatory mediators.The methanol extract was re-dissolved in 100 mL of distilled water and fractionated with chloroform,ethyl acetate and n-butanol.The four fractions(chloroform,ethyl acetate,n-butanol and aqueous)were subjected to anti-inflammatory activity.Chloroform fraction showed good anti-inflammatory activity at dose of 200 mg/kg.Chloroform fraction was then subjected to normal phase silica gel column chromatography and eluted with petroleum ether-chloroform,chloroform-ethyl acetate mixtures of increasing polarity which produced 15 fractions(F1-F15).Only fractions F1,F2,F4,F5,F7,F9,F11 and F14 were found to be active,hence these were analyzed with HPTLC to develop their finger print profile.These fractions showed different spots with different R_f values.Conclusions:The different chloroform fractions F1,F2,F4,F5,F7,F9,F11 and F14 revealed 4,7,7,8,9,7,7 and 6 major spots,respectively.The results obtained in this experiment strongly support and validate the traditional uses of this Sudanese medicinal plant.

Anti-inflammatory activity and qualitative analysis of different extracts of Maytenus obscura(A.Rich.) Cuf.by high performance thin layer chromatography method

Objective:To perform aqueous elhanol soluble fraction(AESK) and dichloromethane extract of aerial parts of Maytenus obscura(A.Rich.) Cuf.using high performance thin layer chromatography(HPTLC) and to test anti-inflammatory activity of these extracts.Methods:HPTLC studies were carried out using CAM At;HPTLC system pquipped with Linomat IV applicator,TLC scanner 3.Reprostar 3.CAMAG ADC 2 and WIN CAT5-4 software were used.The anti-inflammatory activity was tested by injecting different groups of rats(6 each) with formalin in hind paw and measuring the edema volume before and 1h later formalin injection.Control group received saline i.p.The extracts treatment was injected i.p.in doses of 100 and 200 mg/kg 1h before formalin administration.Indomethaein(30 mg/kg) was used as standard.Results:The results of preliminary phytochemical studies confirmed the presence of protein,lipid,carbohydrate,phenol,flavonoid.saponin,triterpenoid,alkaloid and anthraquinonc in both extracts.Chromatography was performed on glass-backed silica gel 60 F254 HPTLC plates with the green solvents toluene:ethyacetate:glacial acetic acid(5:3:0.2,v/v/v) as mobile phase.HPTLC.finger printing of AESF revealed major eight peaks with R_f values in the range of 0.28 to 0.80 and the dichloromethane revealed major 11 peaks with R_f values in the range of 0.12 to 0.76.The purity of sample was confirmed by comparing the absorption spectra at start,middle and end position of the hand.Treatment of rats(i.p.) with AESF and dirhloromethane in doses of 100 and 200 mg/kg inhibited singnificantly(P<0.05.n=6) formalin-induced inflammation by 50%.55.9%,45.5%,and51.4%.respectively.Conclusions:HPTLC finger printing of AESF and dichloromethane of Maytenus obscura revealed eight major spots for alcoholic extracts and nine major spots for dichloromethane extracts.These HPTLC profiles may be of great usefulness in the quality control of herbal products containing these extracts.The anti-inflammatory activity of both extracts also revealed the medicinal importance of these extracts.The plant can be further explored for the isolation of phyloconstiluents having anti-inflammatory activity.

无铬型耐指纹热浸镀锌钢板耐指纹膜对产品属性的影响

通过试验研究了在一定的生产工艺下,热浸镀锌产品表面无铬耐指纹处理的不同膜重对该产品的属性,包括导电性、耐摩擦性、耐指纹性、耐热性、涂装性及耐蚀性的影响,并提供相关的技术参考。结果表明,耐指纹膜重是影响产品属性的重要因素,将其控制在1.0～1.3 g/m2范围内,可得到属性优良的耐指纹热浸镀锌产品。

经典名方桃核承气汤抗炎作用的谱效关系研究

目的建立桃核承气汤的HPLC指纹图谱,并研究其与抗炎药效的谱效关系。方法采用HPLC建立15批桃核承气汤的指纹图谱,并进行药效试验,以TNF-α的抑制率和IL-10的促进率为抗炎作用的药效指标,使用灰色关联度分析法构建谱效关系。结果建立了桃核承气HPLC指纹图谱,确定了22共有峰并指认了其中9个色谱峰。药效试验结果显示,各批次桃核承气汤均具有一定的抗炎作用。经灰色关联度分析可知,除2号峰外各共有峰与2个抗炎药效指标的关联度均>0.6,其中4、10(大黄酸-8-O-β-D葡萄糖苷)、16、21(甘草酸)号峰与2个抗炎药效指标的关联度均>0.75。结论通过谱效关系研究明确了桃核承气汤抗炎药效作用是多种成分共同作用的结果。

热轧板锌铝镁机组的设计要点

论述了环境用锌铝镁的工艺过程,以及2种热轧板锌铝镁的生产工艺,同时讨论了感应加热的必要性,其核心设备是镀后上行冷却器,严格满足镀层形核和出花的不同阶段对冷却速度的要求。

毛橘红与光橘红的HPLC药效指纹图谱比较研究

目的:建立能代表毛橘红与光橘红止咳、祛痰、抗炎药效的HPLC指纹图谱,并比较两者的差异。方法:采用小鼠氨水引咳法、酚红排泄法和二甲苯致耳肿胀法对毛橘红和光橘红进行止咳、祛痰和抗炎作用研究。采用HPLC法建立指纹图谱。色谱柱为Shiseido Capcell Pak C18色谱柱(4.6 mm×250 mm,5μm);流动相为甲醇(A)-0.5%醋酸水溶液(B),二元梯度洗脱(0 min,10%A→20%A;10 min,20%A→40%A;30 min,40%A→49%A;85 min,49%A→90%A);检测波长为320 nm;柱温为30℃;流速为1.0 mL·min-1。分别采用"中药色谱指纹图谱相似度评价系统(2012.1版本)"和SPSS 17.0对谱图进行相似度分析和聚类分析。结果:毛橘红与光橘红70%乙醇提取物均具有止咳、祛痰和抗炎作用,毛橘红优于光橘红。毛橘红与光橘红的指纹图谱分别有18和17个共有峰,从对照指纹图谱和聚类分析结果看,两者存在明显差异。结论:建立了基于药效的毛橘红与光橘红70%乙醇提取物的HPLC指纹图谱,该分析方法专属、灵敏、准确,为毛橘红与光橘红的鉴别及该药品有效性质量控制提供参考。毛橘红与光橘红药效成分指纹图谱差异显著,质量标准中可以分别建立毛橘红和光橘红的指纹图谱的检测。