类风湿性关节炎滑膜病变高频超声表现与Anti-ccp、ESR及CRP的相关性

目的分析类风湿性关节炎(RA)滑膜病变高频超声表现与抗环瓜氨酸肽抗体(Anti-CCP)、红细胞沉降率(ESR)和C反应蛋白(CRP)的相关性,以期为该疾病早期诊断及治疗方案的制定提供更为准确和科学的依据。方法选取2020年1月至2023年3月于大庆龙南医院风湿免疫科住院的治疗前RA患者65例(RA组),另选取同期于本院体检中心行体检的健康者55名(对照组)。比较两组高频超声表现、髌上囊液体厚度、滑膜厚度、滑膜动脉RI及实验室指标(Anti-ccp、ESR及CRP水平),RA组根据Anti-ccp、ESR及CRP阳性标准分为阳性、阴性组,比较Anti-ccp、ESR及CRP阳性及阴性组的髌上囊液体厚度、滑膜厚度、滑膜动脉RI。采用Pearson分析滑膜病变高频超声表现各指标与Anti-ccp、ESR及CRP的相关性。结果对照组膝积液少<4 mm;RA组80%膝积液>4 mm,多对称,尤见于内外侧隐窝。对照组滑膜难辨,RA组滑膜增厚、低回声、分界清、表面糙、见绒毛。彩超示RA组81.53%膝滑膜有明显血流信号,多为Ⅱ、Ⅲ级。RA组髌上囊液体厚度、滑膜厚度、Anti-ccp、ESR及CRP水平均高于对照组,滑膜动脉RI则低于对照组,差异有统计学意义(P<0.05);Anti-ccp、ESR及CRP阳性组髌上囊液体厚度、滑膜厚度水平均显著高于阴性组,滑膜动脉RI低于阴性组,差异有统计学意义(P<0.05)。髌上囊液体厚度、滑膜厚度与Anti-ccp、ESR及CRP水平均呈正相关(P<0.05);滑膜动脉RI与Anti-ccp、ESR及CRP水平均呈负相关(P<0.05)。结论高频超声可清晰观察RA患者的滑膜病变情况,且与Anti-ccp、ESR及CRP等实验室指标存在显著关联性,可为临床诊断RA滑膜病变患者提供有力的参考依据,利于制定更具针对性的治疗措施。

Particle agglomeration and inhibition method in the fluidized pyrolysis reaction of waste resin

This work investigated the pyrolysis reaction of waste resin in a fluidized bed reactor.It was found that the pyrolysis-generated ash would adhere to the surface of ceramic particles,causing particle agglomeration and defluidization.Adding kaolin could effectively inhibit the particle agglomeration during the fluidized pyrolysis reaction through physical isolation and chemical reaction.On the one hand,kaolin could form a coating layer on the surface of ceramic particles to prevent the adhesion of organic ash generated by the pyrolysis of resin.On the other hand,when a sufficient amount of kaolin(-0.2%(mass))was added,the activated kaolin could fully contact with the Na+ ions generated by the pyrolysis of resin and react to form a high-melting aluminosilicate mineral(nepheline),which could reduce the formation of low-melting-point sodium sulfate and thereby avoid the agglomeration of ceramic particles.

Agglomeration of coal and polyethylene mixtures during fixed‑bed co‑gasification

The article presents the results of experimental studies on the gasification of mixtures of brown coal and polyethylene(up to 20 wt%fraction)in a laboratory reactor.The work aims to study the agglomeration process during the heating and oxidation of the mixtures.The measurement results(gas composition,pressure drop)provide indirect information on the dynamics of thermal decomposition and structural changes in the fuel bed.We have shown that the interaction between polyethylene and a coal surface leads to the formation of dense agglomerates,in which the molten polymer acts as a binder.Clinkers form as a result of interfacial interactions between components and filtration flow rearranging.The hydrogen/carbon ratio in the solid residue of coal-polyethylene co-gasification increases from 0.07–0.2 to 1.11,indicating the formation of stable hydrocarbon compounds on the carbon surface.The conducted research makes it possible to identify possible interactions between chemical reactions and transfer processes that lead to agglomeration in mixtures of coal with polyethylene.

类风湿关节炎合并骨关节炎病人血清anti-Sa、anti-CCP水平与骨关节损伤、全身炎症反应的相关性分析

目的:分析类风湿关节炎(RA)病人血清anti-Sa、anti-CCP抗体水平与其骨关节损伤、全身炎症反应的相关性。方法:选择RA合并骨关节损伤病人108例,根据血清anti-Sa、anti-CCP抗体水平分别列入高anti-Sa组和低anti-Sa组、高anti-CCP组和低anti-CCP组。比较各组病人WOMAC骨性关节炎指数、lysholms膝关节功能评分值,血清白细胞介素(IL)-1β、IL-6、IL-27、肿瘤坏死因子α(TNF-α)水平。采用Pearson检验评估有关指标的相关性。结果:高anti-Sa组、高anti-CCP组病人的WOMAC骨性关节炎指数明显高于低anti-Sa组(P<0.01),lysholms膝关节功能评分明显低于低anti-Sa组(P<0.01);血清中IL-1β、IL-6、IL-27、TNF-α的水平明显高于低anti-CCP组(P<0.01)。RA病人血清anti-Sa、anti-CCP抗体水平与骨关节损伤、全身炎症反应严重程度呈正相关关系(P<0.05~P<0.01),与lysholms膝关节功能评分呈负相关关系(P<0.01)。结论:RA病人血清中anti-Sa、anti-CCP抗体水平显著升高与关节损伤及全身炎症反应有一定的相关性,可作为临床判断病情活动性与骨关节损伤的参考指标。

血清anti-β2GPI、FSTL1、PD-1对系统性红斑狼疮免疫功能、疾病活动度的影响

目的探究血清抗β2糖蛋白I抗体(anti-β2GPI)、卵泡抑素样蛋白(FSTL1)、程序性死亡受体1(PD-1)对系统性红斑狼疮(SLE)患者免疫功能、疾病活动度的影响。方法选取我院2019年1月~2022年6月SLE患者113例作为观察组,另选取同期健康体检者105例作为对照组。比较两组、不同疾病活动度患者血清anti-β2GPI、FSTL1、PD-1水平,评价各血清指标与疾病活动度的关系,并根据观察组血清表达水平分为高表达者、低表达者,对比两者免疫功能(CD4+、CD8+、CD4+/CD8+),分析各血清指标与免疫功能相关性。结果观察组血清anti-β2GPI、FSTL1、PD-1高于对照组(P<0.05);血清anti-β2GPI、FSTL1、PD-1高表达患者CD4+、CD4+/CD8+低于低表达患者,CD8+高于低表达患者(P<0.05);血清anti-β2GPI、FSTL1、PD-1与CD4+、CD4+/CD8+呈负相关,与CD8+呈正相关(P<0.05);血清anti-β2GPI、FSTL1、PD-1水平随疾病活动度增加呈升高趋势,且重度活动患者>中度活动患者>轻度活动患者>病情稳定患者(P<0.05);血清anti-β2GPI、FSTL1、PD-1与疾病活动度显著相关(P<0.05)。结论血清anti-β2GPI、FSTL1、PD-1高表达可能参与SLE患者细胞免疫功能紊乱、疾病活动度加重的发生过程。

Evaluation and Influencing Factors on Particle Agglomeration in RAP

Asphalt extraction test and scanning electron microscopy(SEM) were used for analysis of agglomerations of reclaimed asphalt pavement(RAP) particles. In order to quantify the agglomeration degree of RAP, the fineness modulus ratio(FMR) and the percentage loss index(PLI) were proposed. In addition, grey correlation analysis was conducted to discuss the relationship between particle agglomerations and RAP size,asphalt content(AC), and surface area. Two indexes indicate that the agglomeration degree increases in general as the RAP size reduces. This can be attributed to that particles are prone to agglomeration in the case of higher AC. Based on the SEM images and the material composition of RAP, the particle agglomeration in RAP can be classified into weak agglomeration and strong agglomeration. Grey correlation analysis shows that AC is the crucial factor affecting the agglomeration degree and RAP variability. In order to produce consistent and stable reclaimed mixtures, disposal measures of RAP are suggested to lower the AC of RAP.

Assessment of Liver Fibrosis in HBsAg-Negative and Anti HBc Positive Patients

Background: Surface antigen (HBsAg) is the mean marker of hepatitis B virus infection. During the course of the infection, some patients lose the HBsAg and only the presence of anti-HBc antibody indicates previous contact with the virus. Among these patients, some have detectable viral load (occult infection) but most without viral replication. There is no guideline regarding these patients. The aim of this study was to assess hepatic fibrosis in patients with only the hepatitis B virus contact marker “total anti-HBc”. Patients and methods: it was a descriptive and analytical cross-sectional study, conducted in three private hospitals from January to August 2022. Were included HBsAg-negative and HBc-positive patients, consulting in Gastroenterology departments. Noninvasive methods (APRI, FIB-4 and FIBROSCAN) were used to evaluate liver stiffness because of their easy accessibility and low-cost. The hepatic fibrosis was considered significant when the score determined by APRI, FIB-4 and FIBROSCAN® tests was respectively greater than 1.5;2.67 and 8 kPa corresponding to fibrosis level 2 (F2). Results: A total of 63 HBsAg-negative/total HBcAg-positive patients were included. The mean age was 49.9 ± 13.4 years. The male/female sex ratio was 1.78. Of the 63 patients, 19 had significant liver fibrosis (30.1%) among which 9 patients had HCC. The FIB-4 score outperformed the APRI score in assessing liver fibrosis, with a sensitivity of 84.2%, a specificity of 100% and a negative predictive value of 93.6%. In univariate analysis, there was a significant association between the occurrence of significant liver fibrosis and age over 40 years, dyslipidaemia, obesity, alcohol consumption, smoking, herbal medicine, negative anti-HBs immunological status and detectable viral load. Conclusion: Our study revealed a high prevalence of significant to severe hepatic fibrosis in anti-HBc positive patients. In most of the cases, the fibrosis was severe. Progression to HCC has also been possible. There is no consensus on the follow-up strategy for those patients. However, screening for hepatic fibrosis using noninvasive methods should be recommended for patients aged over 40 years, alcohol or herbal medicine users, patients with metabolic syndrome or occult hepatitis B. In HBsAg-negative/anti-HBc-positive patients, liver stiffness should be evaluated and if it is greater than F2, HCC screening should be started.

Particle agglomeration and interphase interactions affect Young’s modulus of nanobiocomposites:eyes at mathematical modeling

Computational modeling is a new approach to optimize Young’s modulus of scaffolds by performing a minimal number of experiments.However,presenting a modeling algorithm to predict Young’s modulus and characterize the governing parameters is a challenging task.Here,a novel modeling approach has been proposed to estimate Young’s modulus of scaffolds,considering particle agglomeration and interphase interactions.Employing the characteristic parameters of these two phenomena,we modified the Maxwell model using a simple three-step algorithm to determine the optimal value of these parameters and predict Young’s modulus.Interestingly,the model provides a precision of more than 95%for all the studied cases and presents a remarkably better performance compared to the two other models.For instance,the proposed model has reduced the average absolute relative error of Young’s modulus of poly(3-hydroxybutyrate)-keratin/hydroxyapatite nanorods from 25.1%to 0.08%,demonstrating the high efficiency of this model in predicting Young’s modulus of scaffolds.The results of this study could lighten the way of fabricating nanobiocomposites with optimal mechanical properties,spending lower cost and energy.

A new fluorocarbon adhesive:Inhibiting agglomeration during combustion of propellant via efficient F-Al_(2)O_(3) preignition reaction

Inhibiting the agglomeration of molten aluminum particles packed in the binder network is a promising scheme to achieve efficient combustion of solid propellants.In this investigation,the hydroxyl-terminated structured fluorinated alcohol compound(PFD)was introduced to modify the traditional polyethylene glycol/polytetrahydrofuran block copolymerization(HTPE)binder;that is,a unique fluorinated polyether(FTPE)binder was synthesized by embedding fluorinated organic segments into the HTPE binder via crosslinking curing.The FTPE was applied in aluminum-based propellants for the first time.Due to the complete release of fluorinated organic active segments in the range of 300℃to 400℃,the burning rate of FTPEbased propellant increased from 4.07(0%PFD)to 6.36 mm/s(5%PFD),increased by 56.27%under 1 MPa.The reaction heat of FTPE propellants increased from 5.95(0%PFD)to 7.18 MJ/kg(5%PFD)under 3.0 MPa,indicating that HTPE binder modified with PFD would be conducive to inhibiting the D90 of condensed combustion products(CCPs)dropped by 81.84%from 75.46(0%PFD)to 13.71μm(5%PFD)under 3.0 MPa,in consistent with the significant reduction of aluminum agglomerates observed on the quenched burning surface of the propellants.Those results demonstrated that a novel FTPE binder with PFD can release fluorinated organic active segments,which motivate preignition reaction with the alumina shell in the early stage of aluminum combustion,and then enhance the melting diffusion effect of aluminum to inhibit the agglomeration.

Anti-GM1影响裸鼠NK细胞分化和杀伤活性研究

目的研究抗单唾液酸神经节苷脂抗体(Anti-GM1)通过调节自然杀伤(NK)细胞分化,抑制裸鼠NK细胞的自然杀伤活性。方法多重免疫荧光染色鉴定人肾癌组织中NK细胞的分型特点。选择雄性BALB/c小鼠6只,GM1皮下注射每周1次,一共三周,以此来免疫小鼠制备Anti-GM1。选取6只BALB/c-Nude裸鼠,采用随机数字表法将裸鼠分为对照组(CON组)及实验组(Anti-GM1组),CON组用IgG连续腹腔注射7 d;Anti-GM1组用Anti-GM1连续腹腔注射7 d。7 d后经颈椎脱臼法处死裸鼠,取裸鼠脾脏并制成单细胞悬液,将脾细胞与YAC-1细胞共培养4 h。用LDH法检测NK细胞的杀伤活力。使用流式细胞术进行检测,根据CD27和CD11b表达来界定NK细胞亚群,同时检测CD107a的表达情况。ELISA检测NK细胞的γ干扰素(IFN-γ)及颗粒酶B(GzmB)分泌水平。结果人肾癌组织标本中有明显的NK细胞浸润现象,并且GzmB也呈现阳性表达。Anti-GM1可以显著抑制NK细胞的杀伤活力。与CON组相比,Anti-GM1组可以促进NK细胞向成熟亚群进行分化。Anti-GM1组NK细胞的CD107a水平显著下降。Anti-GM1组NK细胞的IFN-γ及GzmB分泌水平较CON组下降。结论NK细胞可以浸润在人肾癌组织中,并且能够分泌GzmB,发生自然杀伤作用。Anti-GM1可以促进NK细胞的成熟,但抑制NK细胞的杀伤活力,与其抑制NK细胞CD107a表达和抑制NK细胞的IFN-γ及GzmB分泌有关。

Evolution and Determinants of Population Agglomeration in Less Developed Metropolitan Areas:A Case Study of the Taiyuan Metropolitan Area,China

It is of importance to enhance the urban areas'capacity for population aggregation in underdeveloped regions,aiming to rectify the imbalanced and insufficient pattern of economic development in China.Taking the Taiyuan Metropolitan Area(TMA)in central China as a case study,this paper examines the evolutionary process and characteristics of population agglomeration from 2000 to 2020,and identifies factors associated with agglomeration and their spatial effects.The findings indicated that:1)against the background of sustained population shrinkage in the provincial area,the TMA showed a demographic trend of steady increase,albeit with a decelerated growth rate.In the metropolitan area,urban population size continued to grow rapidly,whereas the rural areas endured sustained losses.Disparities in city size continued to widen,and the polarization of concentrated population in the core cities kept increasing.2)Agglomerations in both secondary and service industries had significant positive effects on local population agglomeration,with the former effect being stronger.Regional economic development,government fiscal expenditure,and financial advancement all contributed to facilitating local population clustering.From a spatial spillover perspective,service agglomeration and financial development promoted population agglomeration in surrounding areas.Conversely,fiscal expenditure inhibited such agglomeration.As for industrial agglomeration and regional economic development,their spatial spillover effects were non-significant.The results obtained reveal several policy implications aimed at enhancing the population agglomeration capacity of the metropolitan area in underdeveloped regions during the new era.

Dynamic Development Characteristics and Driving Factors of High Quality Development Level in China’s Five Major Urban Agglomerations

High-quality development is the primary task of comprehensively building a socialist,modern country,as well as the primary task of building urban agglomerations in China.Based on the five development concepts,this paper used the entropy method to measure the High Quality Development Index(HQDI)of the five major urban agglomerations.The results showed that the HQDI of the five major urban agglomerations shows a fluctuating upward trend.First,using the Dagum Gini coefficient to explore the sources of HQDI development differences in urban agglomerations,we found that the main source of HQDI differences in urban agglomerations was inter-regional differences,while intra-regional differences were not important.Second,kernel density estimation was used to test the dynamic evolution trend of HQDI within urban agglomerations.There was a polarisation phenomenon in the HQDI of urban agglomerations,such as the Pearl River Delta urban agglomeration and the Chengdu-Chongqing urban agglomeration.But overall,the degree of imbalance had decreased.Third,using geographic detectors to examine the driving factors of HQDI in urban agglomerations,we found that the main driving forces for improving HQDI in urban agglomerations were economic growth,artificial intelligence technology and fiscal decentralisation.All the interaction factors had greater explanatory power for the spatial differentiation of HQDI,which can be divided into two types:two-factor improvement and non-linear improvement.This study is conducive to improving and enriching the theoretical system for evaluating the high quality development of urban agglomerations,and provides policy references for promoting the high quality development of urban agglomerations.

Analysis of Urban Agglomeration Network Structure Based on Baidu Migration Data: A Case Study of the Guangdong-Hong Kong-Macao Greater Bay Urban Agglomeration

The inter-city linkage heat data provided by Baidu Migration is employed as a characterization of inter-city linkages in order to facilitate the study of the network linkage characteristics and hierarchical structure of urban agglomeration in the Greater Bay Area through the use of social network analysis method.This is the inaugural application of big data based on location services in the study of urban agglomeration network structure,which represents a novel research perspective on this topic.The study reveals that the density of network linkages in the Greater Bay Area urban agglomeration has reached 100%,indicating a mature network-like spatial structure.This structure has given rise to three distinct communities:Shenzhen-Dongguan-Huizhou,Guangzhou-Foshan-Zhaoqing,and Zhuhai-Zhongshan-Jiangmen.Additionally,cities within the Greater Bay Area urban agglomeration play different roles,suggesting that varying development strategies may be necessary to achieve staggered development.The study demonstrates that large datasets represented by LBS can offer novel insights and methodologies for the examination of urban agglomeration network structures,contingent on the appropriate mining and processing of the data.

Spatio-temporal evolution of the coordinated development of high-tech industry agglomeration and resource-environmental carrying capacity in the Yellow River Basin

Under the background of new infrastructure,the Yellow River Basin’s superior growth cannot be separated originating with the synergistic effect of scientific and technological inventiveness and ecological civilization construction.In light of the coupling coordination analysis of the coordination effect of provincial high-tech industry agglomeration and resource carrying capacity in the Yellow River Basin from 2009 to 2021,The evolution of the geographical and temporal pattern of development was investigated using the Moran index and kernel density estimation.The results show that the agglomeration of high-tech industries in the Yellow River Basin presents a development trend of seek improvement in stability,and there is a good coupling and coordination throughout the progression of scientific and technological innovation and the loading capacity of the resource,from the viewpoint of a time series.From the perspective of spatial pattern distribution,the whole basin aims at the lower reaches,accelerates the optimization of digital industry and promotes Yellow River Basin development of superior quality through innovation support and increase of input,and based on policy guidance.

Tumor-infiltrating T-Lymphocyte immunity-related immune tolerance and anti–programmed cell death protein 1/ligand of programmed cell death protein 1 therapy for advanced hepatocellular carcinoma

Systemic therapy has become the standard treatment for patients with advanced hepatocellular carcinoma(HCC)whose treatment options are limited.However,the long-term patient response to drugs and the survival outcomes remain a concern.With increasing exploration of the HCC microenvironment,particularly in terms of T lymphocyte immunity,a new era of immunomolecular targeted therapy,based on molecular signaling,has arrived for advanced HCC.In the study of immune tolerance of the intrinsic HCC microenvironment,we found that multiple immunosuppressive mechanisms and immune checkpoint inhibitors,such as anti–programmed cell death protein 1/ligand of programmed cell death protein 1 therapy,have improved clinical outcomes in some patients with advanced HCC.Furthermore,various combination therapies have been investigated,and HCC types have been categorized into different types based on anti–programmed cell death protein 1(PD-1)/ligand of programmed cell death protein 1(PD-L1)treatment.In this paper,we first discuss the tumor-infiltrating T lymphocyte immunity and immune tolerance of HCC.We then clarify the basic mechanism of anti–PD-1/PD-L1 therapy and discuss the types of HCC based on anti–PD-1/PD-L1 therapy.Thereafter,we explain the relevant studies and mechanisms of combination therapy of anti–PD-1/PD-L1 with antiangiogenesis drugs or multikinase kinase inhibitors,anti–T lymphocyte–related signaling pathways in HCC,and other anti-CD8+T cell immune checkpoints.In this way,this review offers a deeper understanding of anti–PD-1/PD-L1 immunotherapy for advanced HCC,in order to provide better individualized treatments for patients with advanced HCC.

Land Use Dynamics and Ecosystem Service Value Changes in the Yangtze River Delta Urban Agglomeration Under Different Scenarios

Urban agglomerations,serving as pivotal centers of human activity,undergo swift alterations in ecosystem services prompted by shifts in land utilization.Strengthening the monitoring of ecosystem services in present and future urban agglomerations contributes to the rational planning of these areas and enhances the well-being of their inhabitants.Here,we analyzed land use conversion in the Yangtze River Delta(YRD)urban agglomeration during 1990-2020 and discussed the spatiotemporal response and main drivers of changes in ecosystem service value(ESV).By considering the different development strategic directions described in land use planning policies,we predicted land use conversion and its impact on ESV using the Future Land Use Simulation(FLUS)model in three scenari-os in 2025 and 2030.Results show that:1)from 1990 to 2020,land use change is mainly manifested as the continuous expansion of con-struction land to cultivated land.Among the reduced cultivated land,82.2%were occupied by construction land.2)The land use types conversion caused a loss of 21.85 billion yuan(RMB)in ESV during 1990-2020.Moreover,the large reduction of cultivated land area led to the continuous decline of food production value,accounting for 13%of the total ESV loss.3)From 2020 to 2030,land use change will mainly focus on Yangzhou and Zhenjiang in central Jiangsu Province and Taizhou in southern Zhejiang Province.Under the BAU(natural development)and ED(cultivated land protection)scenarios,construction land expansion remains dominant.In contrast,under the EP(ecological protection)scenario,the areas of water bodies and forest land increase significantly.Among the different scenarios,ESV is highest in the EP scenario,making it the optimal solution for sustainable land use.It can be seen that the space use conflict among urban,agriculture and ecology is a key factor leading to ESV change in the urban agglomeration of Yangtze River Delta.There-fore,it is crucial to maintain spatial land use coordination.Our findings provide suggestions for scientific and rational land use planning for the urban agglomeration.

Antibody Pattern: Correlation of Age and Gender in ANA and Anti-ds-DNA Prevalence

The study was designed to find the prevalence of ANA antibodies and anti-dsDNA antibodies in samples tested at AFIP Rawalpindi and their correlation with age and gender and to find positive and negative predictive values of ANA antibodies.For this purpose,twelve thousand nine hundred sixty-seven(12,967)patients were analyzed for ANA with four hundred sixty-eight(468)healthy samples tested as control and four thousand seven hundred three(4,703)patients tested for ds-DNA antibodies.Retrospective data of all samples tested by indirect immunofluorescence(IF)for ANA antibodies and dsDNA antibodies was collected.To address positive and negative predictive values another control group(autoimmunity not suspected)of serum samples was taken from the healthy population.For the first group,age,gender,ANA antibodies and ds-DNA antibodies results(both tests performed by IIF)data was collected from a computer record cell;for the second control group,ANA antibodies were performed by IIF.12,967 and 4,703 samples(Group 1)were tested for ANA antibodies and dsDNA antibodies,respectively,during this period.1,119(9%)and 99(2%)were found positive for ANA antibodies and ds DNA antibodies.Among these positive samples,850(76%)and 73(74%)were females respectively.Gender predisposition towards autoimmunity(ANA)was found significant with a P value of(P=0.001).Relation of age was also found significant with anti-ANA antibodies with a P value of(P=0.001).This study shows a negative correlation between age(P=0.025)and gender(P=0.001)with anti-dsDNA which is also significant.High prevalence was found below the mean age of 38 years(SD±16.635)for ANA antibodies and the mean age of 35 years(SD±15.066)for ds-DNA antibodies.The age of ANA antibodies and dsDNA antibodies positive patients ranged from 1 year old to 98 years old and 2 years old to 95 years old respectively.In the second(autoimmunity-free)control group,a total of 468 samples were tested for ANA antibodies and 9(2%)were found positive.Positive predictive value(PPV)was 8.6%and negative predictive value(NPV)was 98%.ANA is a sensitive test for autoimmunity and it is significantly related to female gender and increasing age.The low prevalence of ANA antibodies among clinically suspected cases suggests that rationalization of test prescriptions is needed.Anti-ds-DNA is also a sensitive test for diagnosis of SLE and it is significantly related to female gender and increasing age.

基于特征信息融合预测Anti-CRISPR蛋白

CRISPR-Cas系统是一种存在于细菌和古细菌中的获得性免疫系统,作为基因编辑工具在癌症治疗等方面被广泛研究,但CRISPR-Cas基因编辑技术存在基因脱靶效应。研究发现,Anti-CRISPR蛋白是一种可以调节CRISPR-Cas系统功能的蛋白质,在不破坏靶向基因编辑的情况下,可以减少脱靶效应等不良影响,从而提高基因编辑技术的效率和安全性。因此,研究Anti-CRISPR蛋白对于理解CRISPR-Cas系统的功能和细菌-病毒相互作用具有重要意义。本文构建了Anti-CRISPR蛋白数据集,提取氨基酸组分、氨基酸二肽组分、g-gap二肽组成、蛋白质二级结构、平均化学位移和蛋白质骨架6种特征参数,利用支持向量机对Anti-CRISPRs蛋白进行预测,在Jackknife检验下,单特征参数最高预测成功率为93.50%;对维度过高的氨基酸二肽组分和g-gap二肽组成分别进行降维处理,得到g-gap二肽组成在g=3、维数是121维时预测成功率最高,为95.10%,进一步研究发现有16种g-gap二肽组合对应11种氨基酸与Anti-CRISPR蛋白预测相关度较大;最后对特征参数进行融合,融合后最高预测成功率为96.07%。

基于YOLOv5s-AntiUAV的反无人机目标检测算法研究

随着无人机的应用领域不断拓展,无人机的“黑飞”给公共安全造成严重损害。为解决侵入式无人机小目标在复杂飞行环境下的错检和漏检问题,提出基于YOLOv5s-AntiUAV的反无人机目标检测算法。首先,引入结合深度超参数卷积的Slim-Neck范式,增强算法特征提取能力并保持计算效率。其次,在骨干和颈部网络引入SPD-Conv模块,提高在低分辨率图像中小目标的检测性能。最后,用Alpha-CIoU替换YOLOv5s算法中的CIoU,增强算法泛用性。YOLOv5s-AntiUAV算法与YOLOv5s、SSD和Faster R-CNN算法在数据集Anti-UAV上的对比实验结果表明,改进算法的mAP@0.5值分别增长了1.1、12.1和4.9个百分点,凸显其实用性。由在VisDrone2019数据集上进行的迁移实验显示,相较于YOLOv5s算法,改进算法mAP@0.5值提升了4.5个百分点,表明其相较于原算法具有更强的鲁棒性。

Study on the Design Scheme of Vehicle Flow Lines in Unilateral Agglomerative Open Service Area

In recent years,with the introduction of the strategy to strengthen the country’s transportation infrastructure and the continuous implementation of new economic development models such as integrating transportation with tourism,various open service areas integrating highway service areas with local tourism have emerged nationwide.Examples include the Yangcheng Lake service area in Jiangsu and the Lengshui service area in Chongqing.This paper focuses on the design example of the Dazu Stone Carving service area on the Chongqing section of the Yurong Expressway,comprehensively considering factors such as construction scale,terrain conditions,and local urban planning.It analyzes and studies the traffic flow design of unilateral agglomerative open service areas around two vehicle traffic conversion links:between the main expressway and the service area,and between the service area and local roads.The aim is to provide a case study reference for similar projects.