Objective:To investigate the phenotype distribution of five antigens of Rh blood group system and the specificity of Rh blood group irregular antibodies in pregnant women with second child.To analyze the relationship ...Objective:To investigate the phenotype distribution of five antigens of Rh blood group system and the specificity of Rh blood group irregular antibodies in pregnant women with second child.To analyze the relationship between Rh blood group antibody and hemolytic disease of the newborn(HDN)in second-child pregnant women,and to provide laboratory basis for the diagnosis and treatment of hemolytic disease of the newborn(Rh-HDN).Methods:500 pregnant women with second child were collected as the study group and 500 pregnant women with first pregnancy as the control group(all pregnant women underwent obstetric examination in the integrated obsteric clinic of our hospital from January 2020 to January 2021).To detectethe Rh blood group antigens(D,C,c,E,e)of the two groups of samples,screene the irregular antibodies,identify the specificity of irregular antibodies,determine the titer and record the hemolytic disease of the newborn of pregnant women with positive Rh blood group antibodies.Results:There were 11 Rh phenotypes in the pregnant women with second child in the study group:CCDee(152cases,30.4%),CcDEe(136cases,27.2%)CcDee(84cases,16.8%),ccDEE(30cases,6%),ccDee(31cases,6.2%),CCDEe(14cases,2.8%),ccDEe(9cases,1.8%),cc dee(18cases,3.6%),CCDEE(2cases,0.4%),CcdEe(12cases,2.4%),Ccdee(6cases,1.2%),CCd ee(6cases,1.2%).A total of 42 cases(8.4%)in the pregnant women with second child were negative for RhD.There were 10 Rh phenotypes in the pregnant women with first pregnancy in the control group:CCDee(144cases,28.8%),CcDEe(138cases,27.6%),CcDee(90cases,18%),ccDEE(42cases,8.4%),ccDee(28cases,5.6%),CCDEe(10cases,2%),ccDEe(8cases,1.6%),cc dee(19cases,3.8%),CCDEE(1cases,0.2%),CcdEe(11cases,2.2%),Ccdee(9cases,1.8%).A total of 39 cases(7.8%)in the pregnant women with first pregnancy were negative for RhD.In the pregnant women with second child in the study group,the positive rate of irregular antibody screening was 4.0%(20/500),and the specificity of Rh blood group antibodies was found as follows:anti-E 1.8%(9/500),anti-D 1.4%(7/500),anti-C 0.4%(2/500)and anti-Ec 0.4%(2/500).The positive rate of irregular antibody screening in the pregnant women with first pregnancy in the control group was 0,and the difference between the two groups was statistically significant(P<0.05).Rh-HDN was found in 10 newborns(2%)of the 20 women with positive irregular antibodies in the pregnant women with second child,and the antibody titer during pregnancy was more than 32.No Rh-HDN occurred in newborns in the pregnant women with first pregnancy,and the difference between the two groups was statistically significant(P<0.05).Conclusion:Pregnancy stimulation can increase the probability of irregular antibodies in pregnant women,and irregular antibodies in Rh blood group can easily cause Rh-HDN,so attention should be paid to routine detection of five antigens of Rh blood group and irregular antibody screening during prenatal examination.It is helpful for the early detection of Rh-blood irregular antibodies and the assessment of fetal or neonatal risk of Rh-HDN.展开更多
A double antibodies additivity ELISA test was employed to identify the epltopes which can be recognized by monoclonal antibodies (McAbs) against IgM from B chronic lymphocyte leukemia (B-CLL). The computer grouping pr...A double antibodies additivity ELISA test was employed to identify the epltopes which can be recognized by monoclonal antibodies (McAbs) against IgM from B chronic lymphocyte leukemia (B-CLL). The computer grouping programme analysis showed that 4 and- isotypic MaAbs could be divided into two groups and 10 anti- idiotype McAbs could be divided into four groups. The result was consistent with that of the indirect sandwich ELISA and inhibition ELISA test. It suggested that there were at least 6 distinct IgM epitopes which can react specifically with 14 McAbs. Our study indicated that the combination of the additivity ELISA test and the computer grouping programme analysis is of help in studying the relationship of the structure and function of antigen.展开更多
AIM： To examine the effects of anti-high mobility group box 1 （HIGB1） neutralizing antibody in experimental severe acute pancreatitis （SAP）. METHODS： SAP was induced by creating closed duodenal loop inC3H/HeN mi...AIM： To examine the effects of anti-high mobility group box 1 （HIGB1） neutralizing antibody in experimental severe acute pancreatitis （SAP）. METHODS： SAP was induced by creating closed duodenal loop inC3H/HeN mice. SAP was induced immediately after intrapedtoneal injection of anti-HMGB1 neutralizing antibody （200 pg）. Sevedty of pancreatitis, organ injury （liver, kidney and lung）, and bacterial translocation to pancreas was examined 12 h after induction of SAP. RESULTS： Anti-HHGB1 neutralizing antibody significantly improved the elevation of the serum amylase level and the histological alterations of pancreas and lung in SAR Anti-HHGB1 antibody also significantly ameliorated the elevations of serum alanine aminotransferase and creatinine in SAR However, anti-HHGB1 antibody worsened the bacterial translocation to pancreas. CONCLUSION： Blockade of HHGB1 attenuated the development of SAP and associated organ dysfunction, suggesting that HHGB1 may act as a key mediator for inflammatory response and organ injury in SAR展开更多
目的对某院住院患者血型意外抗体与疾病特征进行分析,为优化精准输血方案和提高临床输血安全提供参考。方法收集2012年1月~2021年12月西部战区总医院患者意外抗体筛查和鉴定资料,患者年龄、性别、输血史、妊娠史和疾病诊断,分析意外抗...目的对某院住院患者血型意外抗体与疾病特征进行分析,为优化精准输血方案和提高临床输血安全提供参考。方法收集2012年1月~2021年12月西部战区总医院患者意外抗体筛查和鉴定资料,患者年龄、性别、输血史、妊娠史和疾病诊断,分析意外抗体阳性率、构成比及疾病特征。结果患者意外抗体筛查阳性率0.55%(1736/315456),女性高于男性(0.69%vs 0.44%,χ^(2)=90.107,P<0.05),有输血史或(和)妊娠史患者高于无输血史或(和)妊娠史者(75.69%vs 22.81%,χ^(2)=971.098,P<0.05),40~80岁患者占72.93%(1266/1736)。意外抗体阳性患者主要为消化系统疾病、血液及造血器官免疫疾病、肿瘤、泌尿生殖系统疾病、循环系统疾病、肌肉骨骼系统和结缔组织病,占80.41%(1396/1736)。91.88%(1595/1736)抗筛阳性患者做了抗体鉴定,意外抗体以Rh血型系统41.57%(663/1595),Lewis血型系统11.22%(179/1595)和MNS血型系统6.90%(110/1595)为主,抗体特异性以抗-E[32.41%(517/1595)]、抗-Le^(a)[10.47%(167/1595)]和抗-M[6.08%(97/1595)]为主,其他抗体[35.8%(571/1595)]以未检出特异性抗体为主。结论患者血型意外抗体筛查结果与疾病类型分析对输血安全有重要意义,输血科应针对长期输血患者、女性、有妊娠史或输血史患者进行多抗原(Rh Cc DEe,Le^(a),M)匹配的精准输血,降低意外抗体发生率,提高临床输血安全。展开更多
目的调查广西长期反复输血的地中海贫血患者同种异体免疫发生率,并鉴定同种异体抗体特异性,为临床完善输血策略、提高患者生存质量提供依据。方法利用地中海贫血管理系统,收集2017-01/2022-06月在作者医院进行输血治疗的1115例患者的资...目的调查广西长期反复输血的地中海贫血患者同种异体免疫发生率,并鉴定同种异体抗体特异性,为临床完善输血策略、提高患者生存质量提供依据。方法利用地中海贫血管理系统,收集2017-01/2022-06月在作者医院进行输血治疗的1115例患者的资料,统计同种异体抗体阳性发生率,比较不同类型地中海贫血、民族、性别、年龄段以及同种异体抗体阳性发生率,比较不同类型地中海贫血抗体阳性和阴性患者的年龄、Hb水平,对地中海贫血抗体阳性患者抗体进行特异性鉴定。结果1115例地中海贫血患者中有92例(8.25%)抗体筛查阳性。对比重型β地中海贫血(beta-thalassemia major,β-TM)、血红蛋白H病(hemoglobin H disease,HbH),中间型β地中海贫血(beta-thalassemia intermedia,β-TI)的抗体阳性发生率最高;女性抗体阳性发生率高于男性;年龄>20岁患者的抗体阳性发生率最高。β-TM、β-TI、HbH患者中抗体阳性患者的年龄均明显高于抗体阴性患者(P<0.05)。β-TM患者中抗体阳性患者Hb水平低于抗体阴性患者(P<0.05)。地中海贫血抗体阳性患者Rh血型系统抗体及其合并其他抗体占73.92%(68/92),抗-Mur抗体及其合并其他抗体占8.70%(8/92),抗-JK~a、抗-JK~b抗体及其合并其他抗体占7.61%(7/92)。结论反复输血的广西地中海贫血患者,有较高的同种异体抗体阳性发生率,Rh血型系统抗体阳性最多。β-TI患者、汉族患者、女性患者和年龄大于20岁患者更易发生同种异体免疫反应。展开更多
基金Youth Fund Program of Hainan Provincial Natural Science Fundation of China(No.820QN410)。
文摘Objective:To investigate the phenotype distribution of five antigens of Rh blood group system and the specificity of Rh blood group irregular antibodies in pregnant women with second child.To analyze the relationship between Rh blood group antibody and hemolytic disease of the newborn(HDN)in second-child pregnant women,and to provide laboratory basis for the diagnosis and treatment of hemolytic disease of the newborn(Rh-HDN).Methods:500 pregnant women with second child were collected as the study group and 500 pregnant women with first pregnancy as the control group(all pregnant women underwent obstetric examination in the integrated obsteric clinic of our hospital from January 2020 to January 2021).To detectethe Rh blood group antigens(D,C,c,E,e)of the two groups of samples,screene the irregular antibodies,identify the specificity of irregular antibodies,determine the titer and record the hemolytic disease of the newborn of pregnant women with positive Rh blood group antibodies.Results:There were 11 Rh phenotypes in the pregnant women with second child in the study group:CCDee(152cases,30.4%),CcDEe(136cases,27.2%)CcDee(84cases,16.8%),ccDEE(30cases,6%),ccDee(31cases,6.2%),CCDEe(14cases,2.8%),ccDEe(9cases,1.8%),cc dee(18cases,3.6%),CCDEE(2cases,0.4%),CcdEe(12cases,2.4%),Ccdee(6cases,1.2%),CCd ee(6cases,1.2%).A total of 42 cases(8.4%)in the pregnant women with second child were negative for RhD.There were 10 Rh phenotypes in the pregnant women with first pregnancy in the control group:CCDee(144cases,28.8%),CcDEe(138cases,27.6%),CcDee(90cases,18%),ccDEE(42cases,8.4%),ccDee(28cases,5.6%),CCDEe(10cases,2%),ccDEe(8cases,1.6%),cc dee(19cases,3.8%),CCDEE(1cases,0.2%),CcdEe(11cases,2.2%),Ccdee(9cases,1.8%).A total of 39 cases(7.8%)in the pregnant women with first pregnancy were negative for RhD.In the pregnant women with second child in the study group,the positive rate of irregular antibody screening was 4.0%(20/500),and the specificity of Rh blood group antibodies was found as follows:anti-E 1.8%(9/500),anti-D 1.4%(7/500),anti-C 0.4%(2/500)and anti-Ec 0.4%(2/500).The positive rate of irregular antibody screening in the pregnant women with first pregnancy in the control group was 0,and the difference between the two groups was statistically significant(P<0.05).Rh-HDN was found in 10 newborns(2%)of the 20 women with positive irregular antibodies in the pregnant women with second child,and the antibody titer during pregnancy was more than 32.No Rh-HDN occurred in newborns in the pregnant women with first pregnancy,and the difference between the two groups was statistically significant(P<0.05).Conclusion:Pregnancy stimulation can increase the probability of irregular antibodies in pregnant women,and irregular antibodies in Rh blood group can easily cause Rh-HDN,so attention should be paid to routine detection of five antigens of Rh blood group and irregular antibody screening during prenatal examination.It is helpful for the early detection of Rh-blood irregular antibodies and the assessment of fetal or neonatal risk of Rh-HDN.
文摘A double antibodies additivity ELISA test was employed to identify the epltopes which can be recognized by monoclonal antibodies (McAbs) against IgM from B chronic lymphocyte leukemia (B-CLL). The computer grouping programme analysis showed that 4 and- isotypic MaAbs could be divided into two groups and 10 anti- idiotype McAbs could be divided into four groups. The result was consistent with that of the indirect sandwich ELISA and inhibition ELISA test. It suggested that there were at least 6 distinct IgM epitopes which can react specifically with 14 McAbs. Our study indicated that the combination of the additivity ELISA test and the computer grouping programme analysis is of help in studying the relationship of the structure and function of antigen.
基金Supported by Grants-in-Aid for Scientific Research from the Ministry of Education, Science, Sports and Culture of Japan and from the Ministry of Health, Labor and Welfare of Japan
文摘AIM： To examine the effects of anti-high mobility group box 1 （HIGB1） neutralizing antibody in experimental severe acute pancreatitis （SAP）. METHODS： SAP was induced by creating closed duodenal loop inC3H/HeN mice. SAP was induced immediately after intrapedtoneal injection of anti-HMGB1 neutralizing antibody （200 pg）. Sevedty of pancreatitis, organ injury （liver, kidney and lung）, and bacterial translocation to pancreas was examined 12 h after induction of SAP. RESULTS： Anti-HHGB1 neutralizing antibody significantly improved the elevation of the serum amylase level and the histological alterations of pancreas and lung in SAR Anti-HHGB1 antibody also significantly ameliorated the elevations of serum alanine aminotransferase and creatinine in SAR However, anti-HHGB1 antibody worsened the bacterial translocation to pancreas. CONCLUSION： Blockade of HHGB1 attenuated the development of SAP and associated organ dysfunction, suggesting that HHGB1 may act as a key mediator for inflammatory response and organ injury in SAR
文摘目的对某院住院患者血型意外抗体与疾病特征进行分析,为优化精准输血方案和提高临床输血安全提供参考。方法收集2012年1月~2021年12月西部战区总医院患者意外抗体筛查和鉴定资料,患者年龄、性别、输血史、妊娠史和疾病诊断,分析意外抗体阳性率、构成比及疾病特征。结果患者意外抗体筛查阳性率0.55%(1736/315456),女性高于男性(0.69%vs 0.44%,χ^(2)=90.107,P<0.05),有输血史或(和)妊娠史患者高于无输血史或(和)妊娠史者(75.69%vs 22.81%,χ^(2)=971.098,P<0.05),40~80岁患者占72.93%(1266/1736)。意外抗体阳性患者主要为消化系统疾病、血液及造血器官免疫疾病、肿瘤、泌尿生殖系统疾病、循环系统疾病、肌肉骨骼系统和结缔组织病,占80.41%(1396/1736)。91.88%(1595/1736)抗筛阳性患者做了抗体鉴定,意外抗体以Rh血型系统41.57%(663/1595),Lewis血型系统11.22%(179/1595)和MNS血型系统6.90%(110/1595)为主,抗体特异性以抗-E[32.41%(517/1595)]、抗-Le^(a)[10.47%(167/1595)]和抗-M[6.08%(97/1595)]为主,其他抗体[35.8%(571/1595)]以未检出特异性抗体为主。结论患者血型意外抗体筛查结果与疾病类型分析对输血安全有重要意义,输血科应针对长期输血患者、女性、有妊娠史或输血史患者进行多抗原(Rh Cc DEe,Le^(a),M)匹配的精准输血,降低意外抗体发生率,提高临床输血安全。
文摘目的调查广西长期反复输血的地中海贫血患者同种异体免疫发生率,并鉴定同种异体抗体特异性,为临床完善输血策略、提高患者生存质量提供依据。方法利用地中海贫血管理系统,收集2017-01/2022-06月在作者医院进行输血治疗的1115例患者的资料,统计同种异体抗体阳性发生率,比较不同类型地中海贫血、民族、性别、年龄段以及同种异体抗体阳性发生率,比较不同类型地中海贫血抗体阳性和阴性患者的年龄、Hb水平,对地中海贫血抗体阳性患者抗体进行特异性鉴定。结果1115例地中海贫血患者中有92例(8.25%)抗体筛查阳性。对比重型β地中海贫血(beta-thalassemia major,β-TM)、血红蛋白H病(hemoglobin H disease,HbH),中间型β地中海贫血(beta-thalassemia intermedia,β-TI)的抗体阳性发生率最高;女性抗体阳性发生率高于男性;年龄>20岁患者的抗体阳性发生率最高。β-TM、β-TI、HbH患者中抗体阳性患者的年龄均明显高于抗体阴性患者(P<0.05)。β-TM患者中抗体阳性患者Hb水平低于抗体阴性患者(P<0.05)。地中海贫血抗体阳性患者Rh血型系统抗体及其合并其他抗体占73.92%(68/92),抗-Mur抗体及其合并其他抗体占8.70%(8/92),抗-JK~a、抗-JK~b抗体及其合并其他抗体占7.61%(7/92)。结论反复输血的广西地中海贫血患者,有较高的同种异体抗体阳性发生率,Rh血型系统抗体阳性最多。β-TI患者、汉族患者、女性患者和年龄大于20岁患者更易发生同种异体免疫反应。
