Although the enhancing effect of vasopressin on learning and memory has been re-ported by quite a lot of authors, its mechanisms still remain unclear at present. Somereports showed that the central sites of its enhanc...Although the enhancing effect of vasopressin on learning and memory has been re-ported by quite a lot of authors, its mechanisms still remain unclear at present. Somereports showed that the central sites of its enhancing effect are mainly located inlimbic-midbrain structures, inculding hippocampus, septal nucleus, amygdala and raphe nu-cleus, and through these structures AVP exerts its modulatory effect on learning and mem-ory processes. In order to clarify the mechanisms of enhancing effect of 8-arginine-vasopressin (AVP) on discrimination learning in rats, especially the role of endogenousAVP in it, the method of intracerebral microcijection of anti-AVP serum was used in thepresent work.展开更多
文摘Although the enhancing effect of vasopressin on learning and memory has been re-ported by quite a lot of authors, its mechanisms still remain unclear at present. Somereports showed that the central sites of its enhancing effect are mainly located inlimbic-midbrain structures, inculding hippocampus, septal nucleus, amygdala and raphe nu-cleus, and through these structures AVP exerts its modulatory effect on learning and mem-ory processes. In order to clarify the mechanisms of enhancing effect of 8-arginine-vasopressin (AVP) on discrimination learning in rats, especially the role of endogenousAVP in it, the method of intracerebral microcijection of anti-AVP serum was used in thepresent work.
文摘目的:研究芦荟多糖的提取、纯化工艺及体外抗肿瘤活性,为芦荟的产业化开发提供实验依据。方法:采用水提醇沉,三氯乙酸法除蛋白,DEAE-52离子交换色谱分离。用Sephacryl S-400凝胶柱层析纯化并确定芦荟多糖的相对分子量,HPLC法检测纯度,GC法鉴别单糖组成及分子摩尔比。以MTT法观察其体外抗肿瘤活性等。结果:从中华芦荟(Aloe vera L.var. chinesis(Haw.)Berger.)凝胶冻干粉中提取分离得到纯多糖AVP,经HPLC检测为单一对称峰,相对分子量为86000,由甘露糖和葡萄糖组成,摩尔比为10.3∶1.0。AVP对人肝癌细胞(SMMC-7721)、人胃癌细胞(BGC-823)及人肺癌细胞(A549)的增殖均有抑制作用。结论:该法分离的芦荟多糖AVP纯度好,收率高达3.6%,可用于进一步的研究和开发,提高芦荟的资源利用率。
