用免疫磁性阴极选择法从正常人外周血单个核细胞中择取 T 细胞,经 CD28单克隆抗体和(或)乙酰肉豆蔻佛波酯刺激,Western 印渍免疫沉淀反应,白细胞介素-2和白细胞介素-2受体等检测,结果表明 CD28单抗和乙酰肉豆蔻佛波酯复合刺激具有诱导 c...用免疫磁性阴极选择法从正常人外周血单个核细胞中择取 T 细胞,经 CD28单克隆抗体和(或)乙酰肉豆蔻佛波酯刺激,Western 印渍免疫沉淀反应,白细胞介素-2和白细胞介素-2受体等检测,结果表明 CD28单抗和乙酰肉豆蔻佛波酯复合刺激具有诱导 c-Rel 核内移位和磷酸化、增强白细胞介素-2受体表达和白细胞介素-2分泌的作用,证明 CD28信号途径有诱导环孢素 A 抵抗-非钙依赖和环孢素A敏感-钙依赖的传导通路,对 T 细胞活化有重要意义。展开更多
Objective:The aim of this study was to costimulate the CD3-AK cells with anti-CD28 monoclonal antibody (mAb), observe the effect of cell proliferation and cytotoxicity and explore the regulatory role of CD28 mAb on th...Objective:The aim of this study was to costimulate the CD3-AK cells with anti-CD28 monoclonal antibody (mAb), observe the effect of cell proliferation and cytotoxicity and explore the regulatory role of CD28 mAb on the CD3-AK tumoricidal activity. Methods:To prepare CD3-AK cells, observe the number and morphology of the activated T cells with the inverted microscope and detect the inhibitory rate of the myeloma cells by methylthiazolyldiphenyl-tetrazolium bromide (MTT). Results:Adding the anti-CD28 mAb to the CD3-AK cells, the number of cells increased significantly (P < 0.05) and the cytotoxic activity of the cells was significantly higher (P < 0.05). Conclusion:The addition of anti-CD28 mAb has a strong synergistic effect, which can effectively enhance the tumoricidal activity of CD3-AK.展开更多
文摘用免疫磁性阴极选择法从正常人外周血单个核细胞中择取 T 细胞,经 CD28单克隆抗体和(或)乙酰肉豆蔻佛波酯刺激,Western 印渍免疫沉淀反应,白细胞介素-2和白细胞介素-2受体等检测,结果表明 CD28单抗和乙酰肉豆蔻佛波酯复合刺激具有诱导 c-Rel 核内移位和磷酸化、增强白细胞介素-2受体表达和白细胞介素-2分泌的作用,证明 CD28信号途径有诱导环孢素 A 抵抗-非钙依赖和环孢素A敏感-钙依赖的传导通路,对 T 细胞活化有重要意义。
文摘Objective:The aim of this study was to costimulate the CD3-AK cells with anti-CD28 monoclonal antibody (mAb), observe the effect of cell proliferation and cytotoxicity and explore the regulatory role of CD28 mAb on the CD3-AK tumoricidal activity. Methods:To prepare CD3-AK cells, observe the number and morphology of the activated T cells with the inverted microscope and detect the inhibitory rate of the myeloma cells by methylthiazolyldiphenyl-tetrazolium bromide (MTT). Results:Adding the anti-CD28 mAb to the CD3-AK cells, the number of cells increased significantly (P < 0.05) and the cytotoxic activity of the cells was significantly higher (P < 0.05). Conclusion:The addition of anti-CD28 mAb has a strong synergistic effect, which can effectively enhance the tumoricidal activity of CD3-AK.
