目的:调查南京地区中孕孕妇戊型肝炎病毒(hepatitis E virus,HEV)的感染状况。方法:收集临床妊娠监测的中孕孕妇的血清,用酶联免疫吸附试验(ELISA)试剂检测戊型肝炎病毒的抗原、IgG抗体和IgM抗体。结果:在155例中孕孕妇血样中,HEV抗原...目的:调查南京地区中孕孕妇戊型肝炎病毒(hepatitis E virus,HEV)的感染状况。方法:收集临床妊娠监测的中孕孕妇的血清,用酶联免疫吸附试验(ELISA)试剂检测戊型肝炎病毒的抗原、IgG抗体和IgM抗体。结果:在155例中孕孕妇血样中,HEV抗原均呈阴性,1例HEV-IgM抗体阳性,3例HEV-IgG抗体阳性同时其HEV-IgM抗体滴度处于可疑区间,36例为单纯HEV-IgG抗体阳性,115例抗体均阴性。HEV-IgG抗体总的阳性率为25.16%(39/155),与对照组22.22%(20/90)相比,不存在统计学上的差异(P>0.05)。各年龄组之间,随着年龄增长,HEV-IgG抗体阳性率有升高的趋势,无统计学差异(P>0.05)。结论:孕妇戊型肝炎病毒感染现状不容忽视,为保证孕妇和新生儿的安全,建议在妊娠常规监测中增加戊型肝炎血清标志物的检测。展开更多
目的比较两种免疫酶试剂盒recomWell HEV IgG(swine)ELISA(recom Well )和recomLine HEV IgG(swine)(recom Line )检测猪抗E型肝炎病毒(hepatitis E virus,HEV)IgG的准确性。方法分别采用基因3型和基因4型人HEV(human HEV,hHEV)人...目的比较两种免疫酶试剂盒recomWell HEV IgG(swine)ELISA(recom Well )和recomLine HEV IgG(swine)(recom Line )检测猪抗E型肝炎病毒(hepatitis E virus,HEV)IgG的准确性。方法分别采用基因3型和基因4型人HEV(human HEV,hHEV)人工感染无菌猪各2头,采用上述试剂盒检测猪感染后不同天数的血清样品中抗HEV IgG,同时对两头接种PBS液的无菌猪血清样品18份和4头未感染猪的18份血清进行了检测。结果 2头猪人工感染基因3型hHEV后,recomWell检测均于感染后21d出现阳性,并持续56d;recomLine检测,其中1头猪于感染后14d呈阳性,而另1头于感染后21d呈阳性,且均持续56d。2头猪人工感染基因4型hHEV后,两种试剂盒检测,其中1头猪于感染后21d呈阳性,另1头35d呈阳性,并均持续56d仍为阳性。18份对照血清两种试剂盒检测均为阴性。18份未人工感染猪血清中,只有1份两种试剂盒检测均为阳性。两种试剂盒同时检测的共72份样品中,recomWell检出阳性样品23份,阳性率约为32.0%(23/72),recomLine检出阳性样品24份,阳性率约为33.30%(24/72),检出阳性率两者差异不显著(P>0.05);在recomWell检出的23份阳性样品中,recomLine检测均为阳性,两者阳性检出符合率为100%(23/23);recomWell的漏检率约为4.1%(1/24)。结论 recomWellR和recomLine均可用于猪抗HEVIgG的检测,recomLine检测灵敏度略高于recomWell,且操作更简便,不需要特殊昂贵的检测设备,特别适合用于基层检测猪抗HEV IgG。展开更多
To identify the etiology of non-A,non-B,non-C hepatitis,23 such patients succes-sively admitted in our hospital during 1988～1992 were investigated using an enzyme immunoas-say kit with two recombinant hepatitis E vir...To identify the etiology of non-A,non-B,non-C hepatitis,23 such patients succes-sively admitted in our hospital during 1988～1992 were investigated using an enzyme immunoas-say kit with two recombinant hepatitis E virus(HEV)antigens from Genelabs(Redwood City,CA.USA).Anti-HEV-IgG was detected in 18 cases of them.The disease was diagnosed as hep-atitis E.All 18 patients were Guangzhou residents.This is one of the first reports on the pres-ence of sporadic hepatitis E in a metropolis beyond the endemic areas.The present observationshowed that sporadic hepatitis E occurred out of season and was unlike the epidemic form in Xin-jiang.Fifteen of 18 patients aged from 20 to 50 years,suggesting that it usually prevailed amongthe young-to-middle-aged adults.The ratio of male to female was 14 to 4.The ratio of icterictype to anicteric type was 16 to 2.The jaundice of 16 icteric patients disappeared in 24.6 days(range,14～60 days).The average alanine aminotransferase level was 806 IU/L(range,74～1676 IU/L),which declined to normal in 30.4 days(range,19～77 days).All patients recov-ered completely in 4.7 weeks.This report also indicated that the course of sporadic hepatitis E,as of epidemic form,is self-limiting and has no chronic sequelae.展开更多
文摘目的:调查南京地区中孕孕妇戊型肝炎病毒(hepatitis E virus,HEV)的感染状况。方法:收集临床妊娠监测的中孕孕妇的血清,用酶联免疫吸附试验(ELISA)试剂检测戊型肝炎病毒的抗原、IgG抗体和IgM抗体。结果:在155例中孕孕妇血样中,HEV抗原均呈阴性,1例HEV-IgM抗体阳性,3例HEV-IgG抗体阳性同时其HEV-IgM抗体滴度处于可疑区间,36例为单纯HEV-IgG抗体阳性,115例抗体均阴性。HEV-IgG抗体总的阳性率为25.16%(39/155),与对照组22.22%(20/90)相比,不存在统计学上的差异(P>0.05)。各年龄组之间,随着年龄增长,HEV-IgG抗体阳性率有升高的趋势,无统计学差异(P>0.05)。结论:孕妇戊型肝炎病毒感染现状不容忽视,为保证孕妇和新生儿的安全,建议在妊娠常规监测中增加戊型肝炎血清标志物的检测。
文摘目的比较两种免疫酶试剂盒recomWell HEV IgG(swine)ELISA(recom Well )和recomLine HEV IgG(swine)(recom Line )检测猪抗E型肝炎病毒(hepatitis E virus,HEV)IgG的准确性。方法分别采用基因3型和基因4型人HEV(human HEV,hHEV)人工感染无菌猪各2头,采用上述试剂盒检测猪感染后不同天数的血清样品中抗HEV IgG,同时对两头接种PBS液的无菌猪血清样品18份和4头未感染猪的18份血清进行了检测。结果 2头猪人工感染基因3型hHEV后,recomWell检测均于感染后21d出现阳性,并持续56d;recomLine检测,其中1头猪于感染后14d呈阳性,而另1头于感染后21d呈阳性,且均持续56d。2头猪人工感染基因4型hHEV后,两种试剂盒检测,其中1头猪于感染后21d呈阳性,另1头35d呈阳性,并均持续56d仍为阳性。18份对照血清两种试剂盒检测均为阴性。18份未人工感染猪血清中,只有1份两种试剂盒检测均为阳性。两种试剂盒同时检测的共72份样品中,recomWell检出阳性样品23份,阳性率约为32.0%(23/72),recomLine检出阳性样品24份,阳性率约为33.30%(24/72),检出阳性率两者差异不显著(P>0.05);在recomWell检出的23份阳性样品中,recomLine检测均为阳性,两者阳性检出符合率为100%(23/23);recomWell的漏检率约为4.1%(1/24)。结论 recomWellR和recomLine均可用于猪抗HEVIgG的检测,recomLine检测灵敏度略高于recomWell,且操作更简便,不需要特殊昂贵的检测设备,特别适合用于基层检测猪抗HEV IgG。
文摘To identify the etiology of non-A,non-B,non-C hepatitis,23 such patients succes-sively admitted in our hospital during 1988～1992 were investigated using an enzyme immunoas-say kit with two recombinant hepatitis E virus(HEV)antigens from Genelabs(Redwood City,CA.USA).Anti-HEV-IgG was detected in 18 cases of them.The disease was diagnosed as hep-atitis E.All 18 patients were Guangzhou residents.This is one of the first reports on the pres-ence of sporadic hepatitis E in a metropolis beyond the endemic areas.The present observationshowed that sporadic hepatitis E occurred out of season and was unlike the epidemic form in Xin-jiang.Fifteen of 18 patients aged from 20 to 50 years,suggesting that it usually prevailed amongthe young-to-middle-aged adults.The ratio of male to female was 14 to 4.The ratio of icterictype to anicteric type was 16 to 2.The jaundice of 16 icteric patients disappeared in 24.6 days(range,14～60 days).The average alanine aminotransferase level was 806 IU/L(range,74～1676 IU/L),which declined to normal in 30.4 days(range,19～77 days).All patients recov-ered completely in 4.7 weeks.This report also indicated that the course of sporadic hepatitis E,as of epidemic form,is self-limiting and has no chronic sequelae.
